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The map label for this gene is tuf [H]

Identifier: 15920775

GI number: 15920775

Start: 549587

End: 551164

Strand: Direct

Name: tuf [H]

Synonym: ST0557

Alternate gene names: 15920775

Gene position: 549587-551164 (Clockwise)

Preceding gene: 15920773

Following gene: 15920778

Centisome position: 20.39

GC content: 32.89

Gene sequence:

>1578_bases
ATGAACTATCCTAGAGAAAGTGAAGATGGTAAGATTGAGTATAAACTTATTCTTACCAGTAAGGATGAAAGTAGATTACA
AGAGTTAGCGTCTCAAATGAAGTATAGATTAGAAGAAGGAGGAGGAGAGGCTTTCTATATAATTGGTGTTAGCGATAATG
GTGAAGTATTAGGATTAACAGAGAACGAGTTGAATAAGAGCTTAGAAATTCTTTCAAAAATATCATCCATTATTAATGCA
AAGATAGTTCATGTAAGGAAAGTAGAAGCAAAAAAAGGAAAATATATTGCTGAAGTTTTAGTTAGAATTCATAGAGACAA
AATACCGGTACAAGTAAATATTGCAGTCATGGGTCATGTGAATGCTGGCAAAAGCACTTTAACTGGGGCTCTTATTTTTG
GAAAATTAGACGATGGCAATGGTACTTTGAGAGCAATGGTAGCTAAACACGTCCATGAAGTAATATCAGGTAGAACTTCT
TCCATAACACTAAGAGTTCTTGGCTTAAAAGATAATGGGGAAGTAGTAAATTGGAACTTACGAGACCCGTTAGACGAAGC
TGATGTAACACTAAATAGTTCTAAAATTATACGTTTAGTAGATTTAGGTGGTCATGAGAGATATCTAAGAACTACGCTTA
AAGGTTTGTTAGGTTACGAGATAGATTATGTTATGCTCGTTGTAGGATCAGATGACGGTTTATCAGCTATGGGGAAGGAA
CATTTAGCAGTAGCATCTATTTTACATTTTCCAATTTTTATTGTTATAACTAAAATTGATAAATATCCGAAAGAAAGAAT
TAATGAGATAATAACTGATATAAGAAATGTTCTAAAAATTCCTGGAATAAATAGACTTGTAATTGAGGTAGAGAGTGAAG
AAGATTTGTTAAATGCAATTCTTGCTATGAGAACTGGAAGAGTAGTTCCAATATTTAAAGTCTCTAATGTAACTGGAGAA
GGGCTATCTTTGCTTATAAAGTTTCTAAATATGTTACCTCCTAAAAATAAAGTAGAGAAAGTAAGTATTTCGCAACCTCC
CTTAGTTTATATTGATGAGATATATAACGTAACTGGCGTTGGAACTGTTATTTTGGGCTCTGTTATACGCGGGAGTGTAA
ATACGAACGACATTTATTATATTGGTCCTACAAAGTTTGGTGAATTTAAACCAGTCAAAGTAAAGTCTATACAGCTAAAT
AGAATTTTTGTCGATACTGTTTATTCCGGCAGTATTGCGACTTTTGCAATACAAGGAGTTGAAAAAGATACGTTGAGAAA
AGGCATGGTGCTAGTCAAGAATGATCCTAAAGCTCATAAGAAATTTAAGGCAAAAGTAATAGTACTTCATCATCCAACAA
CAATAAGAGAAGGTTATGTAGCAACATTACACCTTTACACTATAAGACAAGCTGTAAAATTTGAAAAAATAACTAAGAAA
TATTTACGTACTGGTGATTCCTCAGAAGTTGTTCTATCTTTTCTTTACAGGCCAGAATACATAGAGAAAGATCAAATATT
TGTGTTTAGAGAAGGAAGAACAAGAGGTCTAGGTATAATAACTGAATTACTAGATTAG

Upstream 100 bases:

>100_bases
AAATGTGTTTTCCTTCATAGAGTAATTTAGGCTCACTCATTTATCTTCAATTATAAAATTTAAACCCTAGTACAAATTAT
TTATTGATAAAAGTGGTGAG

Downstream 100 bases:

>100_bases
CTTACTGCTTGTGCCGTACACTCTAAAATTTGTTTAGTTAATTGTGAGACGTGTTTTTCAACAAAGTTTTTTAATGCGGG
GATTTGAACATCAACTTCGT

Product: GTP-binding protein

Products: GDP; phosphate

Alternate protein names: EF-1-alpha; Elongation factor Tu; EF-Tu [H]

Number of amino acids: Translated: 525; Mature: 525

Protein sequence:

>525_residues
MNYPRESEDGKIEYKLILTSKDESRLQELASQMKYRLEEGGGEAFYIIGVSDNGEVLGLTENELNKSLEILSKISSIINA
KIVHVRKVEAKKGKYIAEVLVRIHRDKIPVQVNIAVMGHVNAGKSTLTGALIFGKLDDGNGTLRAMVAKHVHEVISGRTS
SITLRVLGLKDNGEVVNWNLRDPLDEADVTLNSSKIIRLVDLGGHERYLRTTLKGLLGYEIDYVMLVVGSDDGLSAMGKE
HLAVASILHFPIFIVITKIDKYPKERINEIITDIRNVLKIPGINRLVIEVESEEDLLNAILAMRTGRVVPIFKVSNVTGE
GLSLLIKFLNMLPPKNKVEKVSISQPPLVYIDEIYNVTGVGTVILGSVIRGSVNTNDIYYIGPTKFGEFKPVKVKSIQLN
RIFVDTVYSGSIATFAIQGVEKDTLRKGMVLVKNDPKAHKKFKAKVIVLHHPTTIREGYVATLHLYTIRQAVKFEKITKK
YLRTGDSSEVVLSFLYRPEYIEKDQIFVFREGRTRGLGIITELLD

Sequences:

>Translated_525_residues
MNYPRESEDGKIEYKLILTSKDESRLQELASQMKYRLEEGGGEAFYIIGVSDNGEVLGLTENELNKSLEILSKISSIINA
KIVHVRKVEAKKGKYIAEVLVRIHRDKIPVQVNIAVMGHVNAGKSTLTGALIFGKLDDGNGTLRAMVAKHVHEVISGRTS
SITLRVLGLKDNGEVVNWNLRDPLDEADVTLNSSKIIRLVDLGGHERYLRTTLKGLLGYEIDYVMLVVGSDDGLSAMGKE
HLAVASILHFPIFIVITKIDKYPKERINEIITDIRNVLKIPGINRLVIEVESEEDLLNAILAMRTGRVVPIFKVSNVTGE
GLSLLIKFLNMLPPKNKVEKVSISQPPLVYIDEIYNVTGVGTVILGSVIRGSVNTNDIYYIGPTKFGEFKPVKVKSIQLN
RIFVDTVYSGSIATFAIQGVEKDTLRKGMVLVKNDPKAHKKFKAKVIVLHHPTTIREGYVATLHLYTIRQAVKFEKITKK
YLRTGDSSEVVLSFLYRPEYIEKDQIFVFREGRTRGLGIITELLD
>Mature_525_residues
MNYPRESEDGKIEYKLILTSKDESRLQELASQMKYRLEEGGGEAFYIIGVSDNGEVLGLTENELNKSLEILSKISSIINA
KIVHVRKVEAKKGKYIAEVLVRIHRDKIPVQVNIAVMGHVNAGKSTLTGALIFGKLDDGNGTLRAMVAKHVHEVISGRTS
SITLRVLGLKDNGEVVNWNLRDPLDEADVTLNSSKIIRLVDLGGHERYLRTTLKGLLGYEIDYVMLVVGSDDGLSAMGKE
HLAVASILHFPIFIVITKIDKYPKERINEIITDIRNVLKIPGINRLVIEVESEEDLLNAILAMRTGRVVPIFKVSNVTGE
GLSLLIKFLNMLPPKNKVEKVSISQPPLVYIDEIYNVTGVGTVILGSVIRGSVNTNDIYYIGPTKFGEFKPVKVKSIQLN
RIFVDTVYSGSIATFAIQGVEKDTLRKGMVLVKNDPKAHKKFKAKVIVLHHPTTIREGYVATLHLYTIRQAVKFEKITKK
YLRTGDSSEVVLSFLYRPEYIEKDQIFVFREGRTRGLGIITELLD

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG5258

COG function: function code R; GTPase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI82546879, Length=532, Percent_Identity=39.0977443609023, Blast_Score=378, Evalue=1e-105,
Organism=Homo sapiens, GI45593140, Length=535, Percent_Identity=40, Blast_Score=373, Evalue=1e-103,
Organism=Homo sapiens, GI4503471, Length=432, Percent_Identity=25.2314814814815, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI4503475, Length=414, Percent_Identity=24.1545893719807, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI34147630, Length=450, Percent_Identity=24.8888888888889, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI5729864, Length=383, Percent_Identity=23.7597911227154, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI223555963, Length=383, Percent_Identity=23.7597911227154, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI2367247, Length=240, Percent_Identity=25.8333333333333, Blast_Score=88, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17565438, Length=538, Percent_Identity=37.1747211895911, Blast_Score=336, Evalue=2e-92,
Organism=Caenorhabditis elegans, GI17563834, Length=531, Percent_Identity=35.5932203389831, Blast_Score=305, Evalue=5e-83,
Organism=Caenorhabditis elegans, GI17552884, Length=412, Percent_Identity=24.2718446601942, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17569207, Length=412, Percent_Identity=24.2718446601942, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17556456, Length=439, Percent_Identity=24.6013667425968, Blast_Score=88, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI32566303, Length=402, Percent_Identity=23.134328358209, Blast_Score=82, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI32566629, Length=394, Percent_Identity=24.3654822335025, Blast_Score=80, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI25141371, Length=438, Percent_Identity=23.972602739726, Blast_Score=76, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6324761, Length=437, Percent_Identity=24.2562929061785, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI19922512, Length=516, Percent_Identity=39.1472868217054, Blast_Score=368, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24654852, Length=516, Percent_Identity=39.1472868217054, Blast_Score=368, Evalue=1e-102,
Organism=Drosophila melanogaster, GI28573213, Length=534, Percent_Identity=36.8913857677903, Blast_Score=333, Evalue=2e-91,
Organism=Drosophila melanogaster, GI221378008, Length=534, Percent_Identity=36.8913857677903, Blast_Score=333, Evalue=2e-91,
Organism=Drosophila melanogaster, GI24644462, Length=534, Percent_Identity=36.8913857677903, Blast_Score=333, Evalue=2e-91,
Organism=Drosophila melanogaster, GI24644464, Length=534, Percent_Identity=36.8913857677903, Blast_Score=333, Evalue=2e-91,
Organism=Drosophila melanogaster, GI24644466, Length=534, Percent_Identity=36.8913857677903, Blast_Score=333, Evalue=2e-91,
Organism=Drosophila melanogaster, GI45553807, Length=412, Percent_Identity=25.2427184466019, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI45553816, Length=412, Percent_Identity=25.2427184466019, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24651721, Length=412, Percent_Identity=25.2427184466019, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI17864154, Length=412, Percent_Identity=25.2427184466019, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24652838, Length=417, Percent_Identity=24.4604316546763, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI17137572, Length=417, Percent_Identity=24.4604316546763, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI45550900, Length=411, Percent_Identity=24.8175182481752, Blast_Score=83, Evalue=4e-16,
Organism=Drosophila melanogaster, GI19922690, Length=326, Percent_Identity=26.6871165644172, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI281363316, Length=436, Percent_Identity=23.1651376146789, Blast_Score=80, Evalue=5e-15,
Organism=Drosophila melanogaster, GI17864358, Length=436, Percent_Identity=23.1651376146789, Blast_Score=80, Evalue=5e-15,
Organism=Drosophila melanogaster, GI19921738, Length=455, Percent_Identity=21.978021978022, Blast_Score=74, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004539
- InterPro:   IPR004161
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 58648; Mature: 58648

Theoretical pI: Translated: 9.72; Mature: 9.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYPRESEDGKIEYKLILTSKDESRLQELASQMKYRLEEGGGEAFYIIGVSDNGEVLGLT
CCCCCCCCCCEEEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCEEEEC
ENELNKSLEILSKISSIINAKIVHVRKVEAKKGKYIAEVLVRIHRDKIPVQVNIAVMGHV
HHHHHHHHHHHHHHHHHHCEEEEEEEEECCCCCHHHHHHHHHHHCCCCCEEEEEEEEEEC
NAGKSTLTGALIFGKLDDGNGTLRAMVAKHVHEVISGRTSSITLRVLGLKDNGEVVNWNL
CCCCCCEEEEEEEEEEECCCCHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCEEEEEC
RDPLDEADVTLNSSKIIRLVDLGGHERYLRTTLKGLLGYEIDYVMLVVGSDDGLSAMGKE
CCCCCCCCEEECCCCEEEEEECCCCHHHHHHHHHHHHCCCEEEEEEEEECCCCCHHHCHH
HLAVASILHFPIFIVITKIDKYPKERINEIITDIRNVLKIPGINRLVIEVESEEDLLNAI
HHHHHHHHHHHHEEEEEHHCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHH
LAMRTGRVVPIFKVSNVTGEGLSLLIKFLNMLPPKNKVEKVSISQPPLVYIDEIYNVTGV
HHHHCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCCCEEEECCCCCEEEEEHHCCCCCH
GTVILGSVIRGSVNTNDIYYIGPTKFGEFKPVKVKSIQLNRIFVDTVYSGSIATFAIQGV
HHHHHHHHHCCCCCCCEEEEECCCCCCCCCCEEEEEEEEEEEEEEEEECCCEEEEEEECC
EKDTLRKGMVLVKNDPKAHKKFKAKVIVLHHPTTIREGYVATLHLYTIRQAVKFEKITKK
CHHHHHCCEEEEECCCHHHHCEEEEEEEEECCCCCCCCEEEEEEHHHHHHHHHHHHHHHH
YLRTGDSSEVVLSFLYRPEYIEKDQIFVFREGRTRGLGIITELLD
HHHCCCCHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHCC
>Mature Secondary Structure
MNYPRESEDGKIEYKLILTSKDESRLQELASQMKYRLEEGGGEAFYIIGVSDNGEVLGLT
CCCCCCCCCCEEEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCEEEEC
ENELNKSLEILSKISSIINAKIVHVRKVEAKKGKYIAEVLVRIHRDKIPVQVNIAVMGHV
HHHHHHHHHHHHHHHHHHCEEEEEEEEECCCCCHHHHHHHHHHHCCCCCEEEEEEEEEEC
NAGKSTLTGALIFGKLDDGNGTLRAMVAKHVHEVISGRTSSITLRVLGLKDNGEVVNWNL
CCCCCCEEEEEEEEEEECCCCHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCEEEEEC
RDPLDEADVTLNSSKIIRLVDLGGHERYLRTTLKGLLGYEIDYVMLVVGSDDGLSAMGKE
CCCCCCCCEEECCCCEEEEEECCCCHHHHHHHHHHHHCCCEEEEEEEEECCCCCHHHCHH
HLAVASILHFPIFIVITKIDKYPKERINEIITDIRNVLKIPGINRLVIEVESEEDLLNAI
HHHHHHHHHHHHEEEEEHHCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHH
LAMRTGRVVPIFKVSNVTGEGLSLLIKFLNMLPPKNKVEKVSISQPPLVYIDEIYNVTGV
HHHHCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCCCEEEECCCCCEEEEEHHCCCCCH
GTVILGSVIRGSVNTNDIYYIGPTKFGEFKPVKVKSIQLNRIFVDTVYSGSIATFAIQGV
HHHHHHHHHCCCCCCCEEEEECCCCCCCCCCEEEEEEEEEEEEEEEEECCCEEEEEEECC
EKDTLRKGMVLVKNDPKAHKKFKAKVIVLHHPTTIREGYVATLHLYTIRQAVKFEKITKK
CHHHHHCCEEEEECCCHHHHCEEEEEEEEECCCCCCCCEEEEEEHHHHHHHHHHHHHHHH
YLRTGDSSEVVLSFLYRPEYIEKDQIFVFREGRTRGLGIITELLD
HHHCCCCHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11930014 [H]