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The map label for this gene is bhbA [H]

Identifier: 15920771

GI number: 15920771

Start: 546284

End: 547972

Strand: Reverse

Name: bhbA [H]

Synonym: ST0552

Alternate gene names: 15920771

Gene position: 547972-546284 (Counterclockwise)

Preceding gene: 15920774

Following gene: 15920768

Centisome position: 20.33

GC content: 36.47

Gene sequence:

>1689_bases
ATGCGGGATTTAGATTTGGATACTGAGGAGAAAATTAAAGAAAGTTACCAGCAGTGGGAGGAAAAAGTTCTAAAGCCATG
GTTGAGCAAAAGACCTGAAAGAAAACAATCATTCTCTACTCCCTCGGGAATTGAAGTTAAGAGATTATACACACCATTAG
ACCTTAAAGGTTCTTATGCCGAAAAAATAGGTTTTCCAGGTGAATATCCCTTTACCAGAGGAATTTACCCTACAATGTAT
AGGGGAAGATTATGGACAATTAGACAATATGCAGGGTTTGGATCCGCTGAAGACACAAATGAAAGATTTAGAAAATTACT
ACAAGCAGGACAAACTGGTTTAAGTATGGCATTTGATTTACCAACACAGTTAGGATTAGACCCTGATCATATCTTAGCGT
TTACAGAAGTTGGTGTTGTTGGAGTTTCAATGTTCCATTGGAAAGAAATGGATATAGTGATGAGTGGCATACCTTTAGAT
AAGGTAACTACGTCAATGACAATCAATGCCACAGCAATAGAATTGCTTTCAATGTATGTAGCTACAGCTGAAGCAAGAGG
AATTGACAAGAAAGTTTTAGATGGAACAGTTCAGAATGATATTCTAAAAGAATATATAGCGAGGAAAAACTTTATATATC
CGCCAGAACCGTCTATGAGATATGCAATAGATTTAATAGAATATTCAGCTAAAAACATACCAAAGTGGTATCCAATAAGT
ATTAGTGGATATCACATTAGAGAAGCTGGTGCTGATGCAATACTTGAGGTAGCATTTACACTAGCAGATGGAATTGAGTA
TGTAAGAAAGACAATGGAAAGAGGAATTCCAGTAGATGAATTTGCGCCTAAACTCTCATTCTTCTTCGCTGGATATACTA
ACATTTTTGAAGAAGTGGCAAAGTTTAGGGCGGCAAGGAGAATGTGGGCCAAAATAATGAAAGAATGGTTTGGTGCTAAA
AAACCAGAATCAATGATGTTAAGATTCCACACACAAACTGGCGGTGCCGAGTTAACAGCACAACAGCCAGAAATAAATAT
CATTAGAACTACTTTACAAGCTTTAGCTGCTGTACTAGGAGGAACTCAAAGCTTACACGTAAATTCCTATGATGAAGCCT
TAGCTTTGCCAAGTGAGAAAGCGGCTAAGATTGCTATAAGAGTTCAGCAAATTATTGCGTATGAGAGTGGTGCTGCTGAC
ACAATAGACCCATTAGCTGGATCTTATTACATTGAAGCACTAACAGATGAAATTGAGGAGAAAGCATGGAAAATAATTGA
AAAAATTGAGAGTATGGGGGGCATGATGAAGGCTATTGAAAAAGGTTATCCACAAGCAGAAATTGCTGAAAGTGCTTATA
GGATTCAAAAGAAAATTGAAAGTGGAGAAATGGTTAAAGTAGGTGTAAATATGTTTTATGAACCAGATTGGATTGGAACT
ACTGAAGTATTTAGGGTTAATCCAGAAGTGAGAAATAGAGTTCTAGAGAGATTAAAGAAATATAGAAGCGAGAGGGATGA
AATAAAGTGGAGAGATTCACTAAATATGTTAAGGAAAGCTGCAGAAAAAGAAAATGAAAACTTATTCCCATACATTTTAA
ACGCTATAAAAGCTGGTGCAACAGTAGGAGAGATTAGCGGAACTTTAAGAGAAATATGGGGAGAATATAGGGAACCATCT
ATTTTCTAA

Upstream 100 bases:

>100_bases
TATGATCTATATTTTGTGTCTCCATCAATTGTATTTTTACATTTTTGACTTAAAAGTTTGTTTAAAAAAGCAAAAGTTTT
AAGTGTTTAAACATATATAA

Downstream 100 bases:

>100_bases
GTTTTCTCAAAATATCAAAATATTTTTCCTTTTTATCTATTGGTGCATTTGTGTTTTCTCTTACTACTTCCCATTTATCC
CATGGAAAAATGATCCATTT

Product: coenzyme B12-dependent isobutyryl-CoA mutase

Products: NA

Alternate protein names: MCM [H]

Number of amino acids: Translated: 562; Mature: 562

Protein sequence:

>562_residues
MRDLDLDTEEKIKESYQQWEEKVLKPWLSKRPERKQSFSTPSGIEVKRLYTPLDLKGSYAEKIGFPGEYPFTRGIYPTMY
RGRLWTIRQYAGFGSAEDTNERFRKLLQAGQTGLSMAFDLPTQLGLDPDHILAFTEVGVVGVSMFHWKEMDIVMSGIPLD
KVTTSMTINATAIELLSMYVATAEARGIDKKVLDGTVQNDILKEYIARKNFIYPPEPSMRYAIDLIEYSAKNIPKWYPIS
ISGYHIREAGADAILEVAFTLADGIEYVRKTMERGIPVDEFAPKLSFFFAGYTNIFEEVAKFRAARRMWAKIMKEWFGAK
KPESMMLRFHTQTGGAELTAQQPEINIIRTTLQALAAVLGGTQSLHVNSYDEALALPSEKAAKIAIRVQQIIAYESGAAD
TIDPLAGSYYIEALTDEIEEKAWKIIEKIESMGGMMKAIEKGYPQAEIAESAYRIQKKIESGEMVKVGVNMFYEPDWIGT
TEVFRVNPEVRNRVLERLKKYRSERDEIKWRDSLNMLRKAAEKENENLFPYILNAIKAGATVGEISGTLREIWGEYREPS
IF

Sequences:

>Translated_562_residues
MRDLDLDTEEKIKESYQQWEEKVLKPWLSKRPERKQSFSTPSGIEVKRLYTPLDLKGSYAEKIGFPGEYPFTRGIYPTMY
RGRLWTIRQYAGFGSAEDTNERFRKLLQAGQTGLSMAFDLPTQLGLDPDHILAFTEVGVVGVSMFHWKEMDIVMSGIPLD
KVTTSMTINATAIELLSMYVATAEARGIDKKVLDGTVQNDILKEYIARKNFIYPPEPSMRYAIDLIEYSAKNIPKWYPIS
ISGYHIREAGADAILEVAFTLADGIEYVRKTMERGIPVDEFAPKLSFFFAGYTNIFEEVAKFRAARRMWAKIMKEWFGAK
KPESMMLRFHTQTGGAELTAQQPEINIIRTTLQALAAVLGGTQSLHVNSYDEALALPSEKAAKIAIRVQQIIAYESGAAD
TIDPLAGSYYIEALTDEIEEKAWKIIEKIESMGGMMKAIEKGYPQAEIAESAYRIQKKIESGEMVKVGVNMFYEPDWIGT
TEVFRVNPEVRNRVLERLKKYRSERDEIKWRDSLNMLRKAAEKENENLFPYILNAIKAGATVGEISGTLREIWGEYREPS
IF
>Mature_562_residues
MRDLDLDTEEKIKESYQQWEEKVLKPWLSKRPERKQSFSTPSGIEVKRLYTPLDLKGSYAEKIGFPGEYPFTRGIYPTMY
RGRLWTIRQYAGFGSAEDTNERFRKLLQAGQTGLSMAFDLPTQLGLDPDHILAFTEVGVVGVSMFHWKEMDIVMSGIPLD
KVTTSMTINATAIELLSMYVATAEARGIDKKVLDGTVQNDILKEYIARKNFIYPPEPSMRYAIDLIEYSAKNIPKWYPIS
ISGYHIREAGADAILEVAFTLADGIEYVRKTMERGIPVDEFAPKLSFFFAGYTNIFEEVAKFRAARRMWAKIMKEWFGAK
KPESMMLRFHTQTGGAELTAQQPEINIIRTTLQALAAVLGGTQSLHVNSYDEALALPSEKAAKIAIRVQQIIAYESGAAD
TIDPLAGSYYIEALTDEIEEKAWKIIEKIESMGGMMKAIEKGYPQAEIAESAYRIQKKIESGEMVKVGVNMFYEPDWIGT
TEVFRVNPEVRNRVLERLKKYRSERDEIKWRDSLNMLRKAAEKENENLFPYILNAIKAGATVGEISGTLREIWGEYREPS
IF

Specific function: Catalyzes the interconversion of succinyl-CoA and methylmalonyl-CoA during the polyhydroxyalkanoate degradation pathway [H]

COG id: COG1884

COG function: function code I; Methylmalonyl-CoA mutase, N-terminal domain/subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 B12-binding domain [H]

Homologues:

Organism=Homo sapiens, GI156105689, Length=543, Percent_Identity=46.2246777163904, Blast_Score=488, Evalue=1e-138,
Organism=Escherichia coli, GI1789284, Length=550, Percent_Identity=46.3636363636364, Blast_Score=457, Evalue=1e-129,
Organism=Caenorhabditis elegans, GI25144500, Length=541, Percent_Identity=45.6561922365989, Blast_Score=486, Evalue=1e-137,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006159
- InterPro:   IPR016176
- InterPro:   IPR014348
- InterPro:   IPR006158
- InterPro:   IPR006099
- InterPro:   IPR006098 [H]

Pfam domain/function: PF02310 B12-binding; PF01642 MM_CoA_mutase [H]

EC number: =5.4.99.2 [H]

Molecular weight: Translated: 63975; Mature: 63975

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: PS00228 TUBULIN_B_AUTOREG

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRDLDLDTEEKIKESYQQWEEKVLKPWLSKRPERKQSFSTPSGIEVKRLYTPLDLKGSYA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCEEEEEECCCCCCCCHH
EKIGFPGEYPFTRGIYPTMYRGRLWTIRQYAGFGSAEDTNERFRKLLQAGQTGLSMAFDL
HHCCCCCCCCCCCCCCCHHHCCCEEEEHHHHCCCCCCCHHHHHHHHHHCCCCCCEEEECC
PTQLGLDPDHILAFTEVGVVGVSMFHWKEMDIVMSGIPLDKVTTSMTINATAIELLSMYV
CHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCEEEHHHHHHHHHHHH
ATAEARGIDKKVLDGTVQNDILKEYIARKNFIYPPEPSMRYAIDLIEYSAKNIPKWYPIS
HHHHHCCCCHHHHCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEE
ISGYHIREAGADAILEVAFTLADGIEYVRKTMERGIPVDEFAPKLSFFFAGYTNIFEEVA
ECCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
KFRAARRMWAKIMKEWFGAKKPESMMLRFHTQTGGAELTAQQPEINIIRTTLQALAAVLG
HHHHHHHHHHHHHHHHCCCCCCHHHEEEEEECCCCCEEEECCCCHHHHHHHHHHHHHHHC
GTQSLHVNSYDEALALPSEKAAKIAIRVQQIIAYESGAADTIDPLAGSYYIEALTDEIEE
CCCEEECCCHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
KAWKIIEKIESMGGMMKAIEKGYPQAEIAESAYRIQKKIESGEMVKVGVNMFYEPDWIGT
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEECCCEEECCCCCCC
TEVFRVNPEVRNRVLERLKKYRSERDEIKWRDSLNMLRKAAEKENENLFPYILNAIKAGA
CCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCC
TVGEISGTLREIWGEYREPSIF
CHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MRDLDLDTEEKIKESYQQWEEKVLKPWLSKRPERKQSFSTPSGIEVKRLYTPLDLKGSYA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCEEEEEECCCCCCCCHH
EKIGFPGEYPFTRGIYPTMYRGRLWTIRQYAGFGSAEDTNERFRKLLQAGQTGLSMAFDL
HHCCCCCCCCCCCCCCCHHHCCCEEEEHHHHCCCCCCCHHHHHHHHHHCCCCCCEEEECC
PTQLGLDPDHILAFTEVGVVGVSMFHWKEMDIVMSGIPLDKVTTSMTINATAIELLSMYV
CHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCEEEHHHHHHHHHHHH
ATAEARGIDKKVLDGTVQNDILKEYIARKNFIYPPEPSMRYAIDLIEYSAKNIPKWYPIS
HHHHHCCCCHHHHCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEE
ISGYHIREAGADAILEVAFTLADGIEYVRKTMERGIPVDEFAPKLSFFFAGYTNIFEEVA
ECCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
KFRAARRMWAKIMKEWFGAKKPESMMLRFHTQTGGAELTAQQPEINIIRTTLQALAAVLG
HHHHHHHHHHHHHHHHCCCCCCHHHEEEEEECCCCCEEEECCCCHHHHHHHHHHHHHHHC
GTQSLHVNSYDEALALPSEKAAKIAIRVQQIIAYESGAADTIDPLAGSYYIEALTDEIEE
CCCEEECCCHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
KAWKIIEKIESMGGMMKAIEKGYPQAEIAESAYRIQKKIESGEMVKVGVNMFYEPDWIGT
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEECCCEEECCCCCCC
TEVFRVNPEVRNRVLERLKKYRSERDEIKWRDSLNMLRKAAEKENENLFPYILNAIKAGA
CCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCC
TVGEISGTLREIWGEYREPSIF
CHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9889346; 11481431 [H]