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The map label for this gene is panB

Identifier: 15920749

GI number: 15920749

Start: 528743

End: 529546

Strand: Reverse

Name: panB

Synonym: ST0533

Alternate gene names: 15920749

Gene position: 529546-528743 (Counterclockwise)

Preceding gene: 15920750

Following gene: 15920746

Centisome position: 19.65

GC content: 35.2

Gene sequence:

>804_bases
ATGGAAAAGGTTACAATAAGGGATTTCTTGAAAAAGAAGGAAAAGAAAGAGAAAATAATAATGTTAACAGCCTACGATTA
TCCCTCAGCGAAGATAATATCTCAGACGAATCTTGATGGTATCCTAGTTGGAGACTCCCTTGGAATGGTAGTTTTAGGAA
AAGAGAACACACTTAAAGTGACTATGAGAGATATGTTAATTCATTTAGACGCAGTAGTAAAAGCTAAGCCTCCACAGTTA
ATAGTTGCTGATATGCCATTCTTAAGCTATGAAACGTCAATCAAAGATGCTGTAAAAAATGCCGGATTACTTGCTAGACA
CGGTGCTGATGCAGTAAAATTAGAAGGTGGAGAGGAAATGAGAGATGTTGTTAGGGCTATAGTAAGGGCTGGAATACCTG
TTATGGGTCATATAGGATTAACCCCACAAAGATTCTTAAGAATAGGTGGATATAGAATTTTGGGGAAAAGAGAAAAAGAA
GAGGAACAATTACTTAAAGATGCAAAAACACTAGAAGAAGCTGGGGCTTTTTCAATAGTAATAGAAAACACTTACTCAGA
TGTGGCTAAAAAGATAACTGAAAGCATTAGTATTCCAACGATATGTATAGGAGCAGGACCATATTGTGACGGACAAATTT
TAGTTATTCATGACTTACTTGGTCTTTCAGATTTTACTCCTTATTTTGCAAAAAAATATATAGATTTACGGGGACTAATA
AGAAGAGCAATAGAGGATTATATTTCAGAGGTAAAAGAAGGGAAATTCCCAGGAAAAGAACATTATAAATCTCGAGATAG
CTGA

Upstream 100 bases:

>100_bases
GAAATTTATGAGGAAGCTTCACAATATGAGGAAACATTACCAATTGCTGAAAGAATAAAGAGAATATTAACTTTAGCATC
AATTATTGCTAGGAAAATTT

Downstream 100 bases:

>100_bases
AGAAGGTAGTTACTTATAAATCTAAGACTTTCAGCTAATATTTCCTTGTTATTATAATTTTTCACTATTTGTTCTAATTC
CTCTTTACTTTTGTTTCTTA

Product: 3-methyl-2-oxobutanoate hydroxymethyltransferase

Products: NA

Alternate protein names: Ketopantoate hydroxymethyltransferase; KPHMT

Number of amino acids: Translated: 267; Mature: 267

Protein sequence:

>267_residues
MEKVTIRDFLKKKEKKEKIIMLTAYDYPSAKIISQTNLDGILVGDSLGMVVLGKENTLKVTMRDMLIHLDAVVKAKPPQL
IVADMPFLSYETSIKDAVKNAGLLARHGADAVKLEGGEEMRDVVRAIVRAGIPVMGHIGLTPQRFLRIGGYRILGKREKE
EEQLLKDAKTLEEAGAFSIVIENTYSDVAKKITESISIPTICIGAGPYCDGQILVIHDLLGLSDFTPYFAKKYIDLRGLI
RRAIEDYISEVKEGKFPGKEHYKSRDS

Sequences:

>Translated_267_residues
MEKVTIRDFLKKKEKKEKIIMLTAYDYPSAKIISQTNLDGILVGDSLGMVVLGKENTLKVTMRDMLIHLDAVVKAKPPQL
IVADMPFLSYETSIKDAVKNAGLLARHGADAVKLEGGEEMRDVVRAIVRAGIPVMGHIGLTPQRFLRIGGYRILGKREKE
EEQLLKDAKTLEEAGAFSIVIENTYSDVAKKITESISIPTICIGAGPYCDGQILVIHDLLGLSDFTPYFAKKYIDLRGLI
RRAIEDYISEVKEGKFPGKEHYKSRDS
>Mature_267_residues
MEKVTIRDFLKKKEKKEKIIMLTAYDYPSAKIISQTNLDGILVGDSLGMVVLGKENTLKVTMRDMLIHLDAVVKAKPPQL
IVADMPFLSYETSIKDAVKNAGLLARHGADAVKLEGGEEMRDVVRAIVRAGIPVMGHIGLTPQRFLRIGGYRILGKREKE
EEQLLKDAKTLEEAGAFSIVIENTYSDVAKKITESISIPTICIGAGPYCDGQILVIHDLLGLSDFTPYFAKKYIDLRGLI
RRAIEDYISEVKEGKFPGKEHYKSRDS

Specific function: Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate

COG id: COG0413

COG function: function code H; Ketopantoate hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the panB family

Homologues:

Organism=Escherichia coli, GI1786326, Length=262, Percent_Identity=43.8931297709924, Blast_Score=223, Evalue=1e-59,
Organism=Saccharomyces cerevisiae, GI6319653, Length=273, Percent_Identity=35.1648351648352, Blast_Score=175, Evalue=8e-45,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PANB_SULTO (Q974Y0)

Other databases:

- EMBL:   BA000023
- RefSeq:   NP_376418.1
- ProteinModelPortal:   Q974Y0
- SMR:   Q974Y0
- GeneID:   1458477
- GenomeReviews:   BA000023_GR
- KEGG:   sto:ST0533
- NMPDR:   fig|273063.1.peg.586
- HOGENOM:   HBG299908
- OMA:   DMMIAHG
- ProtClustDB:   PRK00311
- BioCyc:   STOK273063:ST0533-MONOMER
- BRENDA:   2.1.2.11
- GO:   GO:0005737
- HAMAP:   MF_00156
- InterPro:   IPR003700
- InterPro:   IPR015813
- Gene3D:   G3DSA:3.20.20.60
- PANTHER:   PTHR20881
- PIRSF:   PIRSF000388
- TIGRFAMs:   TIGR00222

Pfam domain/function: PF02548 Pantoate_transf; SSF51621 Pyrv/PenolPyrv_Kinase_cat

EC number: =2.1.2.11

Molecular weight: Translated: 29724; Mature: 29724

Theoretical pI: Translated: 8.90; Mature: 8.90

Prosite motif: NA

Important sites: ACT_SITE 182-182 BINDING 84-84 BINDING 113-113

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKVTIRDFLKKKEKKEKIIMLTAYDYPSAKIISQTNLDGILVGDSLGMVVLGKENTLKV
CCCCCHHHHHHHHHCCCEEEEEEECCCCCCEEEEECCCCEEEEECCCCEEEECCCCEEEE
TMRDMLIHLDAVVKAKPPQLIVADMPFLSYETSIKDAVKNAGLLARHGADAVKLEGGEEM
EHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHCCCEEEECCCCEEEECCCHHH
RDVVRAIVRAGIPVMGHIGLTPQRFLRIGGYRILGKREKEEEQLLKDAKTLEEAGAFSIV
HHHHHHHHHCCCCEEECCCCCHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHHCCCEEEE
IENTYSDVAKKITESISIPTICIGAGPYCDGQILVIHDLLGLSDFTPYFAKKYIDLRGLI
EECHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEEHHHCCCCCCHHHHHHHHHHHHHH
RRAIEDYISEVKEGKFPGKEHYKSRDS
HHHHHHHHHHHHCCCCCCHHHCCCCCC
>Mature Secondary Structure
MEKVTIRDFLKKKEKKEKIIMLTAYDYPSAKIISQTNLDGILVGDSLGMVVLGKENTLKV
CCCCCHHHHHHHHHCCCEEEEEEECCCCCCEEEEECCCCEEEEECCCCEEEECCCCEEEE
TMRDMLIHLDAVVKAKPPQLIVADMPFLSYETSIKDAVKNAGLLARHGADAVKLEGGEEM
EHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHCCCEEEECCCCEEEECCCHHH
RDVVRAIVRAGIPVMGHIGLTPQRFLRIGGYRILGKREKEEEQLLKDAKTLEEAGAFSIV
HHHHHHHHHCCCCEEECCCCCHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHHCCCEEEE
IENTYSDVAKKITESISIPTICIGAGPYCDGQILVIHDLLGLSDFTPYFAKKYIDLRGLI
EECHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEEHHHCCCCCCHHHHHHHHHHHHHH
RRAIEDYISEVKEGKFPGKEHYKSRDS
HHHHHHHHHHHHCCCCCCHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11572479