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The map label for this gene is rnhB

Identifier: 15920736

GI number: 15920736

Start: 514734

End: 515360

Strand: Direct

Name: rnhB

Synonym: ST0519

Alternate gene names: 15920736

Gene position: 514734-515360 (Clockwise)

Preceding gene: 15920735

Following gene: 15920737

Centisome position: 19.1

GC content: 30.3

Gene sequence:

>627_bases
ATGATAGTAGTTGGAATTGATGAAGCTGGTAGAGGATCATTAATTGGTCCGATGATTGTTGCTGGAGTTGCTATTAAGAA
TGATAGGTTAAAGTTTTTAAAGAATATAGGTGTGAAAGATAGCAAACAATTAACGAGAAATAGAAGGGAAAAATTGTTTG
ATATTATATGTTCTTATTCCGAGGGATTTACAATAGTAAAAGTCTCACCAGAAGAGATTGATAGTGAAAATCTAAATGAG
TTAACATATAAAGCTATGATAAAAATAATCTATTCTCTAGTAGAATTTAAACCCATTAGAATAAGTATAGATAAAGTTGG
GAATACAAAAGTTGTTGAGCAAGAAATTATAAAACTTGGAATGGAACCTAACATTGTAACTAATGCAGATGTTTATTTTG
TAGAGGCAAGTGCTGCAAGTATTATTGCTAAGGTTATTAGAGATAATATAATTGATACGCTTAAGTCAAAATATGGAGAT
TTTGGTAGTGGTTATCCCTCAGATCCCAAAACAGTAAATTGGGTTAAGAACGTTTATAAGGAATATTTAACTCCGCCTCC
TATCATAAGAAGGAGTTGGAAAATTTTACAAGAGATAGCTCCTAATTATTATATTAGAAAATGGTAA

Upstream 100 bases:

>100_bases
TTACTTCAATGGTAAAAGACGTAAAAATTATGGAAAGTTATCAAGATGAAACAATCAGAAACGGTAAAAAATTTGCTAAA
TTAGTTATATCAGTTAGAGT

Downstream 100 bases:

>100_bases
CTAACTTACCAGCTGAGGCTAAGGCAAAATGGTTAAAATATATGGATGCTAAAACTACAGAGGAGAAAATCAAAGCTCTC
CAAGAATTTTTAAGTGCTGT

Product: ribonuclease HII

Products: NA

Alternate protein names: RNase HII

Number of amino acids: Translated: 208; Mature: 208

Protein sequence:

>208_residues
MIVVGIDEAGRGSLIGPMIVAGVAIKNDRLKFLKNIGVKDSKQLTRNRREKLFDIICSYSEGFTIVKVSPEEIDSENLNE
LTYKAMIKIIYSLVEFKPIRISIDKVGNTKVVEQEIIKLGMEPNIVTNADVYFVEASAASIIAKVIRDNIIDTLKSKYGD
FGSGYPSDPKTVNWVKNVYKEYLTPPPIIRRSWKILQEIAPNYYIRKW

Sequences:

>Translated_208_residues
MIVVGIDEAGRGSLIGPMIVAGVAIKNDRLKFLKNIGVKDSKQLTRNRREKLFDIICSYSEGFTIVKVSPEEIDSENLNE
LTYKAMIKIIYSLVEFKPIRISIDKVGNTKVVEQEIIKLGMEPNIVTNADVYFVEASAASIIAKVIRDNIIDTLKSKYGD
FGSGYPSDPKTVNWVKNVYKEYLTPPPIIRRSWKILQEIAPNYYIRKW
>Mature_208_residues
MIVVGIDEAGRGSLIGPMIVAGVAIKNDRLKFLKNIGVKDSKQLTRNRREKLFDIICSYSEGFTIVKVSPEEIDSENLNE
LTYKAMIKIIYSLVEFKPIRISIDKVGNTKVVEQEIIKLGMEPNIVTNADVYFVEASAASIIAKVIRDNIIDTLKSKYGD
FGSGYPSDPKTVNWVKNVYKEYLTPPPIIRRSWKILQEIAPNYYIRKW

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0164

COG function: function code L; Ribonuclease HII

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase HII family

Homologues:

Organism=Homo sapiens, GI38455391, Length=221, Percent_Identity=32.579185520362, Blast_Score=102, Evalue=3e-22,
Organism=Escherichia coli, GI1786380, Length=170, Percent_Identity=32.9411764705882, Blast_Score=73, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17536263, Length=221, Percent_Identity=31.6742081447964, Blast_Score=96, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6324257, Length=222, Percent_Identity=31.0810810810811, Blast_Score=80, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24580635, Length=218, Percent_Identity=27.9816513761468, Blast_Score=85, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH2_SULTO (Q974Z3)

Other databases:

- EMBL:   BA000023
- RefSeq:   NP_376405.1
- ProteinModelPortal:   Q974Z3
- SMR:   Q974Z3
- GeneID:   1458464
- GenomeReviews:   BA000023_GR
- KEGG:   sto:ST0519
- NMPDR:   fig|273063.1.peg.573
- HOGENOM:   HBG584843
- OMA:   AEHKADE
- ProtClustDB:   PRK00015
- BioCyc:   STOK273063:ST0519-MONOMER
- BRENDA:   3.1.26.4
- GO:   GO:0005737
- HAMAP:   MF_00052_A
- InterPro:   IPR004649
- InterPro:   IPR001352
- InterPro:   IPR020787
- InterPro:   IPR023160
- InterPro:   IPR012337
- Gene3D:   G3DSA:1.10.10.460
- PANTHER:   PTHR10954
- TIGRFAMs:   TIGR00729

Pfam domain/function: PF01351 RNase_HII; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 23619; Mature: 23619

Theoretical pI: Translated: 9.67; Mature: 9.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVVGIDEAGRGSLIGPMIVAGVAIKNDRLKFLKNIGVKDSKQLTRNRREKLFDIICSYS
CEEEECCCCCCCCCHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCC
EGFTIVKVSPEEIDSENLNELTYKAMIKIIYSLVEFKPIRISIDKVGNTKVVEQEIIKLG
CCEEEEEECHHHCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHC
MEPNIVTNADVYFVEASAASIIAKVIRDNIIDTLKSKYGDFGSGYPSDPKTVNWVKNVYK
CCCCEEECCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHH
EYLTPPPIIRRSWKILQEIAPNYYIRKW
HHCCCCHHHHHHHHHHHHHCCCCEEECC
>Mature Secondary Structure
MIVVGIDEAGRGSLIGPMIVAGVAIKNDRLKFLKNIGVKDSKQLTRNRREKLFDIICSYS
CEEEECCCCCCCCCHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCC
EGFTIVKVSPEEIDSENLNELTYKAMIKIIYSLVEFKPIRISIDKVGNTKVVEQEIIKLG
CCEEEEEECHHHCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHC
MEPNIVTNADVYFVEASAASIIAKVIRDNIIDTLKSKYGDFGSGYPSDPKTVNWVKNVYK
CCCCEEECCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHH
EYLTPPPIIRRSWKILQEIAPNYYIRKW
HHCCCCHHHHHHHHHHHHHCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11572479