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The map label for this gene is cobB

Identifier: 15920592

GI number: 15920592

Start: 402695

End: 404008

Strand: Direct

Name: cobB

Synonym: ST0389

Alternate gene names: 15920592

Gene position: 402695-404008 (Clockwise)

Preceding gene: 15920591

Following gene: 15920594

Centisome position: 14.94

GC content: 31.28

Gene sequence:

>1314_bases
ATGAGGTTTCCTCGTATAATCATTTCTTCAGATAGAAGTAACTCGGGCAAAACAATAATATCCTCTGCCCTAATGAGAGT
TTTATCTAGGAAAATGAAAGTGAGGGGCTTTAAGGCAGGACCAGATTTTATTGATCCAAAATATCATACACTAGCTGCTA
GAGTTCCTTCGATTAATTTAGACTTATGGTTAATGGGAATTGAGGGAGTAAAGAAGAGTTTAATAAGATATGGGAAAGGC
TATGATATTGGAATAATTGAGGGAGTAATGGGATTATATGATGGAATTAACGTGAATTATAGCACTTATGAACTTTCTGA
AGTTACTAAGACCCCGATAATTCTAGTAGTAAACTGTAGTAACGTTAGTAGTACTGTAGGAGCTATAGTAAAAGGATTAA
AAGATTATAGAAACGCAAGAATTAGAGGAGTAATTTTCAATCAAATAGGGTCTGAAACGCATTATAATTACTGTAAGAGT
TCTATAAAAGAAGTTCAAGTTTTAGGTTATATAAAATACGATAGAAATTTCTCTGTCCCTTCACGGCATTTAGGGCTTTT
TACAACTGAGGATTTCAAAGAGACTGAAAATGTTTTACAATCCGTATCTAAAGCTATAGAAGAGTCTGTTGACATAGATA
AAATTATTGAAATTGCTAACTCAGCAGAAGAATTACAGGAAGTAGATGAGGCTATATCTAATGATGAATTAGATACTAAA
AAAGGAATAGCTGCAATAGCATATGATTCAGCATTTAATTTTTATTATTCAGAAAATATAGATTTATTAAGATATAAATA
TCAAATAGAATTTTTCAGTCCTTTATTAAATGAAAAAATTGATAATCCAAGTTTAATTTATGTTGGTGGCGGCTATCCAG
AATTACATCTAAATGAACTTGAGAAATCTAGTAGTACCATAAGATGGATTAAAAAAGAAGCCGAAAAAGGTACAAAAATT
TTGGCAGAGTGTGGGGGTCTAATGTATTTATCGAAAGAAATTATAGCTGATAAAAGCTATAAAATGGTTAATTTGTTTGA
TATTAGCATTAAGGCTAAGGACAAACTCACGATTGGTTATACGGAGCTAGATGTTTTATCAGATAATATACTAGGAAGGA
AAGGAGAGGTTCTTAGAGGTCACGAATTTCATGTATCTAAAGCTATTAATTTAGGTAACGATGTAAAATTCTCTATGAAA
AATAGAATAGGGAAAGGAATATGGGAAAACAAAGATGGAGCCATTGTCTATAATACATTAGCGTCTTATTCTCATTTTCA
TTTTTCAAGTGCTAGAGGATTGTTGTCTTTTTAA

Upstream 100 bases:

>100_bases
AAAGCCTGAATAATAGCCTTCAGATCCCTTACGTCATCGTAAACTATATGCATTTACTATCCCATATTTATTTAATCTAA
TCAGTTTCTTATTTTTTCTT

Downstream 100 bases:

>100_bases
TTCAAGTTTCTTTACTCTATCTAGTACTTCTAATAACCTATCTATTTCTGTGGTGTATAGTCTAATTCTGTATGGCAATT
TAGATGTTTGAGAATGAATT

Product: cobyrinic acid a,c-diamide synthase

Products: ADP; phosphate; Hydrogenobyrinic acid a,c diamide; L-glutamate

Alternate protein names: NA

Number of amino acids: Translated: 437; Mature: 437

Protein sequence:

>437_residues
MRFPRIIISSDRSNSGKTIISSALMRVLSRKMKVRGFKAGPDFIDPKYHTLAARVPSINLDLWLMGIEGVKKSLIRYGKG
YDIGIIEGVMGLYDGINVNYSTYELSEVTKTPIILVVNCSNVSSTVGAIVKGLKDYRNARIRGVIFNQIGSETHYNYCKS
SIKEVQVLGYIKYDRNFSVPSRHLGLFTTEDFKETENVLQSVSKAIEESVDIDKIIEIANSAEELQEVDEAISNDELDTK
KGIAAIAYDSAFNFYYSENIDLLRYKYQIEFFSPLLNEKIDNPSLIYVGGGYPELHLNELEKSSSTIRWIKKEAEKGTKI
LAECGGLMYLSKEIIADKSYKMVNLFDISIKAKDKLTIGYTELDVLSDNILGRKGEVLRGHEFHVSKAINLGNDVKFSMK
NRIGKGIWENKDGAIVYNTLASYSHFHFSSARGLLSF

Sequences:

>Translated_437_residues
MRFPRIIISSDRSNSGKTIISSALMRVLSRKMKVRGFKAGPDFIDPKYHTLAARVPSINLDLWLMGIEGVKKSLIRYGKG
YDIGIIEGVMGLYDGINVNYSTYELSEVTKTPIILVVNCSNVSSTVGAIVKGLKDYRNARIRGVIFNQIGSETHYNYCKS
SIKEVQVLGYIKYDRNFSVPSRHLGLFTTEDFKETENVLQSVSKAIEESVDIDKIIEIANSAEELQEVDEAISNDELDTK
KGIAAIAYDSAFNFYYSENIDLLRYKYQIEFFSPLLNEKIDNPSLIYVGGGYPELHLNELEKSSSTIRWIKKEAEKGTKI
LAECGGLMYLSKEIIADKSYKMVNLFDISIKAKDKLTIGYTELDVLSDNILGRKGEVLRGHEFHVSKAINLGNDVKFSMK
NRIGKGIWENKDGAIVYNTLASYSHFHFSSARGLLSF
>Mature_437_residues
MRFPRIIISSDRSNSGKTIISSALMRVLSRKMKVRGFKAGPDFIDPKYHTLAARVPSINLDLWLMGIEGVKKSLIRYGKG
YDIGIIEGVMGLYDGINVNYSTYELSEVTKTPIILVVNCSNVSSTVGAIVKGLKDYRNARIRGVIFNQIGSETHYNYCKS
SIKEVQVLGYIKYDRNFSVPSRHLGLFTTEDFKETENVLQSVSKAIEESVDIDKIIEIANSAEELQEVDEAISNDELDTK
KGIAAIAYDSAFNFYYSENIDLLRYKYQIEFFSPLLNEKIDNPSLIYVGGGYPELHLNELEKSSSTIRWIKKEAEKGTKI
LAECGGLMYLSKEIIADKSYKMVNLFDISIKAKDKLTIGYTELDVLSDNILGRKGEVLRGHEFHVSKAINLGNDVKFSMK
NRIGKGIWENKDGAIVYNTLASYSHFHFSSARGLLSF

Specific function: Responsible for the amidation of carboxylic groups at position A and C of either cobyrinic acid or hydrogenobrynic acid. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation

COG id: COG1797

COG function: function code H; Cobyrinic acid a,c-diamide synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GATase cobBQ-type domain

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COBB_SULTO (Q975N0)

Other databases:

- EMBL:   BA000023
- RefSeq:   NP_376261.1
- ProteinModelPortal:   Q975N0
- GeneID:   1458314
- GenomeReviews:   BA000023_GR
- KEGG:   sto:ST0389
- NMPDR:   fig|273063.1.peg.429
- HOGENOM:   HBG634910
- ProtClustDB:   CLSK883637
- BioCyc:   STOK273063:ST0389-MONOMER
- HAMAP:   MF_00027
- InterPro:   IPR002586
- InterPro:   IPR004484
- InterPro:   IPR017929
- InterPro:   IPR011698
- TIGRFAMs:   TIGR00379

Pfam domain/function: PF01656 CbiA; PF07685 GATase_3

EC number: 6.3.5.9

Molecular weight: Translated: 49162; Mature: 49162

Theoretical pI: Translated: 8.34; Mature: 8.34

Prosite motif: PS51274 GATASE_COBBQ; PS00445 FGGY_KINASES_2

Important sites: ACT_SITE 324-324 ACT_SITE 425-425

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFPRIIISSDRSNSGKTIISSALMRVLSRKMKVRGFKAGPDFIDPKYHTLAARVPSINL
CCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCE
DLWLMGIEGVKKSLIRYGKGYDIGIIEGVMGLYDGINVNYSTYELSEVTKTPIILVVNCS
EEEEEEHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCEEEEEHHHHCCCCEEEEEECC
NVSSTVGAIVKGLKDYRNARIRGVIFNQIGSETHYNYCKSSIKEVQVLGYIKYDRNFSVP
CCHHHHHHHHHHHHHHHCCEEEEEEEHHCCCCHHHHHHHHHHHHEEEEEEEEECCCCCCC
SRHLGLFTTEDFKETENVLQSVSKAIEESVDIDKIIEIANSAEELQEVDEAISNDELDTK
CHHCCCEECHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHCCCCCCHH
KGIAAIAYDSAFNFYYSENIDLLRYKYQIEFFSPLLNEKIDNPSLIYVGGGYPELHLNEL
CCEEEEEECCEEEEEEECCCEEEEEEEEHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHH
EKSSSTIRWIKKEAEKGTKILAECGGLMYLSKEIIADKSYKMVNLFDISIKAKDKLTIGY
HHCCHHHHHHHHHHHHHHHHHHHCCCHHEEHHHHHCCCCCEEEEEEEEEEEECCEEEEEE
TELDVLSDNILGRKGEVLRGHEFHVSKAINLGNDVKFSMKNRIGKGIWENKDGAIVYNTL
EHHHHHHHHHCCCCCCEECCCEEEHHHHHCCCCCEEEHHHHHHCCCCCCCCCCCEEEEEH
ASYSHFHFSSARGLLSF
HCCCCEEHHCCCCCCCC
>Mature Secondary Structure
MRFPRIIISSDRSNSGKTIISSALMRVLSRKMKVRGFKAGPDFIDPKYHTLAARVPSINL
CCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCE
DLWLMGIEGVKKSLIRYGKGYDIGIIEGVMGLYDGINVNYSTYELSEVTKTPIILVVNCS
EEEEEEHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCEEEEEHHHHCCCCEEEEEECC
NVSSTVGAIVKGLKDYRNARIRGVIFNQIGSETHYNYCKSSIKEVQVLGYIKYDRNFSVP
CCHHHHHHHHHHHHHHHCCEEEEEEEHHCCCCHHHHHHHHHHHHEEEEEEEEECCCCCCC
SRHLGLFTTEDFKETENVLQSVSKAIEESVDIDKIIEIANSAEELQEVDEAISNDELDTK
CHHCCCEECHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHCCCCCCHH
KGIAAIAYDSAFNFYYSENIDLLRYKYQIEFFSPLLNEKIDNPSLIYVGGGYPELHLNEL
CCEEEEEECCEEEEEEECCCEEEEEEEEHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHH
EKSSSTIRWIKKEAEKGTKILAECGGLMYLSKEIIADKSYKMVNLFDISIKAKDKLTIGY
HHCCHHHHHHHHHHHHHHHHHHHCCCHHEEHHHHHCCCCCEEEEEEEEEEEECCEEEEEE
TELDVLSDNILGRKGEVLRGHEFHVSKAINLGNDVKFSMKNRIGKGIWENKDGAIVYNTL
EHHHHHHHHHCCCCCCEECCCEEEHHHHHCCCCCEEEHHHHHHCCCCCCCCCCCEEEEEH
ASYSHFHFSSARGLLSF
HCCCCEEHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; hydrogenobyrinic acid; L-glutamine; H2O

Specific reaction: 2 ATP + hydrogenobyrinic acid + 2 L-glutamine + 2 H2O = 2 ADP + 2 phosphate + hydrogenobyrinic acid a,c-diamide + 2 L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11572479