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The map label for this gene is tfb

Identifier: 15920551

GI number: 15920551

Start: 371631

End: 372557

Strand: Direct

Name: tfb

Synonym: ST0349

Alternate gene names: 15920551

Gene position: 371631-372557 (Clockwise)

Preceding gene: 15920548

Following gene: 161751101

Centisome position: 13.79

GC content: 36.03

Gene sequence:

>927_bases
ATGTCAGAAAGTAATAAATCTCAAGCTTCTGCTACTCCTTGTCCTCCAGATAAAATAGTTTTTGATGAGGAAAGGGGAGA
GTATATTTGTACGGAAACTGGGGAAGTGATAGAAGAAAGGGCAATTGATCAGGGGCCCGAATGGAGGGCTTTCACTCCAG
AAGAAAAAGAGAAAAGAAGTAGAGTTGGAGGTCCATTAAATCAGACTATACATGATATGGGTATATCTACTGTAATTGAT
TGGAAAGACAAAGACGCAATGGGTAGAACTTTAGATCCAAAGAGGAGATTAGAAGTATTAAGATGGAGAAAGTGGCAAAT
TAGAGCAAGAATACAATCTTCTATAGATAGAAACTTAGCACAAGCAATGAATGAGTTAGAGAGAATAGGAAACTTGCTTA
ATTTACCAAAGGCTGTAAAAGATGAGGCTGCTTTAATTTATAGAAAAGCTGTTGAGAAAGGATTAGTAAGAGGCAGAAGT
ATTGAAAGTGTTGTTGCAGCATCTATATATGCAGCTTGTAGAAGAATGAAGATGGCAAGGACATTAGATGAAATTGCTCA
ATTTACAAAGGCTAACAGAAAAGAAGTAGCTAGATGTTATAGATTAATTTTAAGAGAATTAGATATTAATGTCCCTGTTA
GTGATCCAAAGGATTATGTTACTAGAATTGGTAGTTTGTTAGGCTTAAGCGGTTCTACAATGAAGATGGCAATAGATATT
ATTGAGAAAGCTAAAGAGTCTGGTCTAACGGCTGGAAAAGATCCTGCTGGCCTGGCAGCTGCTGCAATTTATATAGCGGC
CTTACTTAATGATGAAAGAAGGACGCAGAAAGAGATTGCACAGATTGCAGGTGTTACAGAAGTAACTGTTAGAAATAGAT
ATAAGGAATTAACACAAGAGTTAAAAATTCAAATTCCAAATCAATAA

Upstream 100 bases:

>100_bases
ATTTCTTCTATATTCATTAAATACTAGAAAAGTTTATATATACGTATAAGCATAAAGTTTATAAGGGGTTTTAAAAAGTT
TCCCTCTTCTGGGTGGTTTT

Downstream 100 bases:

>100_bases
AATTTTAACTTTTTTCTTAGTTTAACTCTTTTTATTTTCTTTCTTTTTATTCTATTATTATGATGTTAGATTCTGGGTAA
CCCATTTTCATAAGAAGTTC

Product: transcription initiation factor IIB

Products: NA

Alternate protein names: TFIIB

Number of amino acids: Translated: 308; Mature: 307

Protein sequence:

>308_residues
MSESNKSQASATPCPPDKIVFDEERGEYICTETGEVIEERAIDQGPEWRAFTPEEKEKRSRVGGPLNQTIHDMGISTVID
WKDKDAMGRTLDPKRRLEVLRWRKWQIRARIQSSIDRNLAQAMNELERIGNLLNLPKAVKDEAALIYRKAVEKGLVRGRS
IESVVAASIYAACRRMKMARTLDEIAQFTKANRKEVARCYRLILRELDINVPVSDPKDYVTRIGSLLGLSGSTMKMAIDI
IEKAKESGLTAGKDPAGLAAAAIYIAALLNDERRTQKEIAQIAGVTEVTVRNRYKELTQELKIQIPNQ

Sequences:

>Translated_308_residues
MSESNKSQASATPCPPDKIVFDEERGEYICTETGEVIEERAIDQGPEWRAFTPEEKEKRSRVGGPLNQTIHDMGISTVID
WKDKDAMGRTLDPKRRLEVLRWRKWQIRARIQSSIDRNLAQAMNELERIGNLLNLPKAVKDEAALIYRKAVEKGLVRGRS
IESVVAASIYAACRRMKMARTLDEIAQFTKANRKEVARCYRLILRELDINVPVSDPKDYVTRIGSLLGLSGSTMKMAIDI
IEKAKESGLTAGKDPAGLAAAAIYIAALLNDERRTQKEIAQIAGVTEVTVRNRYKELTQELKIQIPNQ
>Mature_307_residues
SESNKSQASATPCPPDKIVFDEERGEYICTETGEVIEERAIDQGPEWRAFTPEEKEKRSRVGGPLNQTIHDMGISTVIDW
KDKDAMGRTLDPKRRLEVLRWRKWQIRARIQSSIDRNLAQAMNELERIGNLLNLPKAVKDEAALIYRKAVEKGLVRGRSI
ESVVAASIYAACRRMKMARTLDEIAQFTKANRKEVARCYRLILRELDINVPVSDPKDYVTRIGSLLGLSGSTMKMAIDII
EKAKESGLTAGKDPAGLAAAAIYIAALLNDERRTQKEIAQIAGVTEVTVRNRYKELTQELKIQIPNQ

Specific function: Stabilizes TBP binding to an archaeal box-A promoter. Also responsible for recruiting RNA polymerase II to the pre- initiation complex (DNA-TBP-TFIIB)

COG id: COG1405

COG function: function code K; Transcription initiation factor TFIIIB, Brf1 subunit/Transcription initiation factor TFIIB

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TFIIB family

Homologues:

Organism=Homo sapiens, GI4504193, Length=286, Percent_Identity=35.3146853146853, Blast_Score=171, Evalue=7e-43,
Organism=Homo sapiens, GI22035556, Length=287, Percent_Identity=27.5261324041812, Blast_Score=103, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17564892, Length=290, Percent_Identity=31.0344827586207, Blast_Score=158, Evalue=3e-39,
Organism=Caenorhabditis elegans, GI17531397, Length=289, Percent_Identity=23.8754325259516, Blast_Score=91, Evalue=7e-19,
Organism=Saccharomyces cerevisiae, GI6325343, Length=309, Percent_Identity=29.4498381877023, Blast_Score=105, Evalue=8e-24,
Organism=Saccharomyces cerevisiae, GI6321685, Length=183, Percent_Identity=26.2295081967213, Blast_Score=86, Evalue=9e-18,
Organism=Drosophila melanogaster, GI17136758, Length=294, Percent_Identity=34.6938775510204, Blast_Score=174, Evalue=7e-44,
Organism=Drosophila melanogaster, GI281361961, Length=162, Percent_Identity=29.6296296296296, Blast_Score=85, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24647658, Length=162, Percent_Identity=29.6296296296296, Blast_Score=85, Evalue=6e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TF2B_SULTO (Q975S1)

Other databases:

- EMBL:   BA000023
- RefSeq:   NP_376220.1
- ProteinModelPortal:   Q975S1
- GeneID:   1458273
- GenomeReviews:   BA000023_GR
- KEGG:   sto:ST0349
- NMPDR:   fig|273063.1.peg.388
- HOGENOM:   HBG646536
- OMA:   HDYERAE
- ProtClustDB:   PRK00423
- BioCyc:   STOK273063:ST0349-MONOMER
- HAMAP:   MF_00383
- InterPro:   IPR006670
- InterPro:   IPR011028
- InterPro:   IPR013763
- InterPro:   IPR000812
- InterPro:   IPR013150
- InterPro:   IPR013137
- Gene3D:   G3DSA:1.10.472.10
- PANTHER:   PTHR11618
- PRINTS:   PR00685
- SMART:   SM00385

Pfam domain/function: PF00382 TFIIB; PF08271 TFIIB_Zn_Ribbon; SSF47954 Cyclin_like

EC number: NA

Molecular weight: Translated: 34665; Mature: 34534

Theoretical pI: Translated: 9.49; Mature: 9.49

Prosite motif: PS00782 TFIIB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSESNKSQASATPCPPDKIVFDEERGEYICTETGEVIEERAIDQGPEWRAFTPEEKEKRS
CCCCCCCCCCCCCCCCCCEEEECCCCCEEECCHHHHHHHHHHCCCCCCCCCCCHHHHHHH
RVGGPLNQTIHDMGISTVIDWKDKDAMGRTLDPKRRLEVLRWRKWQIRARIQSSIDRNLA
HCCCCHHHHHHHCCCHHEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QAMNELERIGNLLNLPKAVKDEAALIYRKAVEKGLVRGRSIESVVAASIYAACRRMKMAR
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
TLDEIAQFTKANRKEVARCYRLILRELDINVPVSDPKDYVTRIGSLLGLSGSTMKMAIDI
HHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHH
IEKAKESGLTAGKDPAGLAAAAIYIAALLNDERRTQKEIAQIAGVTEVTVRNRYKELTQE
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
LKIQIPNQ
HHHCCCCC
>Mature Secondary Structure 
SESNKSQASATPCPPDKIVFDEERGEYICTETGEVIEERAIDQGPEWRAFTPEEKEKRS
CCCCCCCCCCCCCCCCCEEEECCCCCEEECCHHHHHHHHHHCCCCCCCCCCCHHHHHHH
RVGGPLNQTIHDMGISTVIDWKDKDAMGRTLDPKRRLEVLRWRKWQIRARIQSSIDRNLA
HCCCCHHHHHHHCCCHHEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QAMNELERIGNLLNLPKAVKDEAALIYRKAVEKGLVRGRSIESVVAASIYAACRRMKMAR
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
TLDEIAQFTKANRKEVARCYRLILRELDINVPVSDPKDYVTRIGSLLGLSGSTMKMAIDI
HHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHH
IEKAKESGLTAGKDPAGLAAAAIYIAALLNDERRTQKEIAQIAGVTEVTVRNRYKELTQE
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
LKIQIPNQ
HHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11572479