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The map label for this gene is aspC

Identifier: 15920386

GI number: 15920386

Start: 227743

End: 229032

Strand: Direct

Name: aspC

Synonym: ST0205

Alternate gene names: 15920386

Gene position: 227743-229032 (Clockwise)

Preceding gene: 15920385

Following gene: 15920390

Centisome position: 8.45

GC content: 34.03

Gene sequence:

>1290_bases
ATGTATAGAAGTCATTTCATTGCTGATGTAACTCCAGAATATGATGGAAAAGAAGTAATATGGGCTGGATGGGTTCATCT
TTTGCGCGACTTAGGTGGTAAGAAATTTATAATACTTAGAGATAAGACTGGTTTAGGTCAAGTAGTAGTAGACAAAAACT
CTAGTGCTTTTGGTATTTCGCAAGAACTTACACAAGAAAGTGTTATCCAAGTGAGAGGTATAGTAAAAGCTGATAAAAGA
GCTCCTAGAGGAATAGAACTCCATGCAGAAGAAATAACTTTACTAAGTAAAGCAAAAGCACCTTTGCCTTTAGATGTTTC
TGGTAAAGTAAAAGCTGATATAGATACTAGACTTAGAGAGAGAGTATTAGATTTAAGAAGACAAGAAATGCAAGCAGTAA
TAAAAATCCAATCTTTAGCACTAAAGGCTTTTAGAGAAACATTATATAAAGAAGGATTTATTGAAATTTTCACCCCAAAG
ATTATAGCATCAGCTACTGAGGGTGGTGCACAATTATTTCCCGTGATATACTTTGGTAAGGAGGCATTTTTAGCGCAAAG
CCCACAGCTTTATAAAGAGCTTATGGCTGGAGTAGTAGAAAGAGTTTTTGAAGTTGCTCCTGCTTGGAGAGCTGAAGAAT
CTGATACACCGTTTCATTTAGCAGAATTTATTAGTATGGATGTAGAAATGGCGTTTGCTGATTATAACGATGTAATGCAA
CTTTTAGAGAAAATATTACATAATATTGTAAAGACCATAAAAGAAGAAGGAAAAGAAGAGCTAAAAATTTTAAATTATGA
ACCACCAGAAGTAAAGATACCTATTAAAAGATTAAAGTACACTGAAGCAATAGAAATCTTAAGAAGTAAAGGATACAATA
TTAAATTTGGAGATGATATAGGAACACCCGAATTAAGAATACTTAATGAGGAACTAAAAGAGGATTTATATTTCATTGTT
GATTGGCCCTCAGATGCAAGACCATTCTATACTAAAAGTAAAAGTGAGAACCCAGAATTAAGCGAAAGTTTCGATTTAAT
CTATAAATTTTTAGAAATAGTATCAGGAAGCACAAGAAATCATAAGAGAGAAGTACTAGAAGAAGCATTAAAGAAAAAAG
GATTGAAACCAGAGAGCTTCGAGTTTTTCCTAAAATGGTTTGATTATGGAATGCCACCTCACGCCGGATTTGGAATGGGA
CTAGCAAGGCTAATGGTAATGTTAACGGGAATACAGAGCGTTAAGGAAATAGTTCCCTTCCCTAGAGATAAGAAAAGACT
AACACCCTAA

Upstream 100 bases:

>100_bases
CTAGAAATGAAAAGAGATTTCTACCAGCAGATGAAAATTCGTTAGAAACAGCACTATCAAAAATCCATGCTATCCCAATA
AAAATTAGGGTGGAGTAAAA

Downstream 100 bases:

>100_bases
GTAAAAAGTCTTGGATCAGTTCTCGCAATCTCTCTTATAACAATAGTTGCATAAATTCCACGATCTAAAGCAAATGTAAT
TTTTTTATCTTTTATGGTTA

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MYRSHFIADVTPEYDGKEVIWAGWVHLLRDLGGKKFIILRDKTGLGQVVVDKNSSAFGISQELTQESVIQVRGIVKADKR
APRGIELHAEEITLLSKAKAPLPLDVSGKVKADIDTRLRERVLDLRRQEMQAVIKIQSLALKAFRETLYKEGFIEIFTPK
IIASATEGGAQLFPVIYFGKEAFLAQSPQLYKELMAGVVERVFEVAPAWRAEESDTPFHLAEFISMDVEMAFADYNDVMQ
LLEKILHNIVKTIKEEGKEELKILNYEPPEVKIPIKRLKYTEAIEILRSKGYNIKFGDDIGTPELRILNEELKEDLYFIV
DWPSDARPFYTKSKSENPELSESFDLIYKFLEIVSGSTRNHKREVLEEALKKKGLKPESFEFFLKWFDYGMPPHAGFGMG
LARLMVMLTGIQSVKEIVPFPRDKKRLTP

Sequences:

>Translated_429_residues
MYRSHFIADVTPEYDGKEVIWAGWVHLLRDLGGKKFIILRDKTGLGQVVVDKNSSAFGISQELTQESVIQVRGIVKADKR
APRGIELHAEEITLLSKAKAPLPLDVSGKVKADIDTRLRERVLDLRRQEMQAVIKIQSLALKAFRETLYKEGFIEIFTPK
IIASATEGGAQLFPVIYFGKEAFLAQSPQLYKELMAGVVERVFEVAPAWRAEESDTPFHLAEFISMDVEMAFADYNDVMQ
LLEKILHNIVKTIKEEGKEELKILNYEPPEVKIPIKRLKYTEAIEILRSKGYNIKFGDDIGTPELRILNEELKEDLYFIV
DWPSDARPFYTKSKSENPELSESFDLIYKFLEIVSGSTRNHKREVLEEALKKKGLKPESFEFFLKWFDYGMPPHAGFGMG
LARLMVMLTGIQSVKEIVPFPRDKKRLTP
>Mature_429_residues
MYRSHFIADVTPEYDGKEVIWAGWVHLLRDLGGKKFIILRDKTGLGQVVVDKNSSAFGISQELTQESVIQVRGIVKADKR
APRGIELHAEEITLLSKAKAPLPLDVSGKVKADIDTRLRERVLDLRRQEMQAVIKIQSLALKAFRETLYKEGFIEIFTPK
IIASATEGGAQLFPVIYFGKEAFLAQSPQLYKELMAGVVERVFEVAPAWRAEESDTPFHLAEFISMDVEMAFADYNDVMQ
LLEKILHNIVKTIKEEGKEELKILNYEPPEVKIPIKRLKYTEAIEILRSKGYNIKFGDDIGTPELRILNEELKEDLYFIV
DWPSDARPFYTKSKSENPELSESFDLIYKFLEIVSGSTRNHKREVLEEALKKKGLKPESFEFFLKWFDYGMPPHAGFGMG
LARLMVMLTGIQSVKEIVPFPRDKKRLTP

Specific function: Unknown

COG id: COG0017

COG function: function code J; Aspartyl/asparaginyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI45439306, Length=444, Percent_Identity=37.1621621621622, Blast_Score=284, Evalue=9e-77,
Organism=Homo sapiens, GI4758762, Length=430, Percent_Identity=30.9302325581395, Blast_Score=216, Evalue=4e-56,
Organism=Homo sapiens, GI226491502, Length=437, Percent_Identity=27.0022883295195, Blast_Score=167, Evalue=2e-41,
Organism=Homo sapiens, GI40789249, Length=259, Percent_Identity=33.976833976834, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI5031815, Length=460, Percent_Identity=29.3478260869565, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI194272210, Length=460, Percent_Identity=29.3478260869565, Blast_Score=117, Evalue=2e-26,
Organism=Escherichia coli, GI1787161, Length=450, Percent_Identity=27.7777777777778, Blast_Score=171, Evalue=1e-43,
Organism=Escherichia coli, GI1789256, Length=468, Percent_Identity=26.0683760683761, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI1790571, Length=466, Percent_Identity=24.8927038626609, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI1788173, Length=326, Percent_Identity=28.8343558282209, Blast_Score=112, Evalue=6e-26,
Organism=Caenorhabditis elegans, GI17551876, Length=450, Percent_Identity=33.5555555555556, Blast_Score=251, Evalue=8e-67,
Organism=Caenorhabditis elegans, GI71984122, Length=422, Percent_Identity=30.3317535545024, Blast_Score=196, Evalue=2e-50,
Organism=Caenorhabditis elegans, GI17556372, Length=403, Percent_Identity=26.5508684863524, Blast_Score=121, Evalue=7e-28,
Organism=Caenorhabditis elegans, GI32566633, Length=330, Percent_Identity=29.3939393939394, Blast_Score=115, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI71994340, Length=459, Percent_Identity=28.1045751633987, Blast_Score=104, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI17535925, Length=459, Percent_Identity=28.1045751633987, Blast_Score=104, Evalue=8e-23,
Organism=Caenorhabditis elegans, GI17535927, Length=459, Percent_Identity=28.1045751633987, Blast_Score=104, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6323011, Length=452, Percent_Identity=34.2920353982301, Blast_Score=246, Evalue=7e-66,
Organism=Saccharomyces cerevisiae, GI6321807, Length=429, Percent_Identity=30.7692307692308, Blast_Score=199, Evalue=5e-52,
Organism=Saccharomyces cerevisiae, GI6319872, Length=344, Percent_Identity=28.4883720930233, Blast_Score=139, Evalue=6e-34,
Organism=Saccharomyces cerevisiae, GI6320242, Length=468, Percent_Identity=25.8547008547009, Blast_Score=97, Evalue=6e-21,
Organism=Saccharomyces cerevisiae, GI6325153, Length=311, Percent_Identity=28.2958199356913, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI17136276, Length=455, Percent_Identity=34.0659340659341, Blast_Score=257, Evalue=1e-68,
Organism=Drosophila melanogaster, GI19921528, Length=421, Percent_Identity=31.5914489311164, Blast_Score=189, Evalue=4e-48,
Organism=Drosophila melanogaster, GI19922416, Length=449, Percent_Identity=27.1714922048998, Blast_Score=163, Evalue=2e-40,
Organism=Drosophila melanogaster, GI24584738, Length=310, Percent_Identity=30.3225806451613, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24640851, Length=458, Percent_Identity=28.1659388646288, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24640849, Length=458, Percent_Identity=28.3842794759825, Blast_Score=116, Evalue=2e-26,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): SYD_SULTO (Q976I3)

Other databases:

- EMBL:   BA000023
- RefSeq:   NP_376055.1
- PDB:   1WYD
- PDBsum:   1WYD
- ProteinModelPortal:   Q976I3
- SMR:   Q976I3
- GeneID:   1458094
- GenomeReviews:   BA000023_GR
- KEGG:   sto:ST0205
- NMPDR:   fig|273063.1.peg.223
- HOGENOM:   HBG745843
- OMA:   ASAFPRD
- ProtClustDB:   PRK05159
- BioCyc:   STOK273063:ST0205-MONOMER
- BRENDA:   6.1.1.12
- GO:   GO:0005737
- HAMAP:   MF_00044_A
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020780
- InterPro:   IPR004523
- InterPro:   IPR002312
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR22594
- PRINTS:   PR01042
- TIGRFAMs:   TIGR00458

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =6.1.1.12

Molecular weight: Translated: 49075; Mature: 49075

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYRSHFIADVTPEYDGKEVIWAGWVHLLRDLGGKKFIILRDKTGLGQVVVDKNSSAFGIS
CCCCCEEEECCCCCCCCEEHHHHHHHHHHHCCCCEEEEEECCCCCCEEEEECCCCCCCCH
QELTQESVIQVRGIVKADKRAPRGIELHAEEITLLSKAKAPLPLDVSGKVKADIDTRLRE
HHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHCCCCCCCCCCCCEECCHHHHHHH
RVLDLRRQEMQAVIKIQSLALKAFRETLYKEGFIEIFTPKIIASATEGGAQLFPVIYFGK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECHHHHHHCCCCCCEEEEEEEECC
EAFLAQSPQLYKELMAGVVERVFEVAPAWRAEESDTPFHLAEFISMDVEMAFADYNDVMQ
HHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHH
LLEKILHNIVKTIKEEGKEELKILNYEPPEVKIPIKRLKYTEAIEILRSKGYNIKFGDDI
HHHHHHHHHHHHHHHCCHHHEEEECCCCCCEECCHHHHHHHHHHHHHHHCCCEEEECCCC
GTPELRILNEELKEDLYFIVDWPSDARPFYTKSKSENPELSESFDLIYKFLEIVSGSTRN
CCCHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCH
HKREVLEEALKKKGLKPESFEFFLKWFDYGMPPHAGFGMGLARLMVMLTGIQSVKEIVPF
HHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
PRDKKRLTP
CCCCCCCCC
>Mature Secondary Structure
MYRSHFIADVTPEYDGKEVIWAGWVHLLRDLGGKKFIILRDKTGLGQVVVDKNSSAFGIS
CCCCCEEEECCCCCCCCEEHHHHHHHHHHHCCCCEEEEEECCCCCCEEEEECCCCCCCCH
QELTQESVIQVRGIVKADKRAPRGIELHAEEITLLSKAKAPLPLDVSGKVKADIDTRLRE
HHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHCCCCCCCCCCCCEECCHHHHHHH
RVLDLRRQEMQAVIKIQSLALKAFRETLYKEGFIEIFTPKIIASATEGGAQLFPVIYFGK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECHHHHHHCCCCCCEEEEEEEECC
EAFLAQSPQLYKELMAGVVERVFEVAPAWRAEESDTPFHLAEFISMDVEMAFADYNDVMQ
HHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHH
LLEKILHNIVKTIKEEGKEELKILNYEPPEVKIPIKRLKYTEAIEILRSKGYNIKFGDDI
HHHHHHHHHHHHHHHCCHHHEEEECCCCCCEECCHHHHHHHHHHHHHHHCCCEEEECCCC
GTPELRILNEELKEDLYFIVDWPSDARPFYTKSKSENPELSESFDLIYKFLEIVSGSTRN
CCCHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCH
HKREVLEEALKKKGLKPESFEFFLKWFDYGMPPHAGFGMGLARLMVMLTGIQSVKEIVPF
HHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
PRDKKRLTP
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11572479