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The map label for this gene is pcm [H]

Identifier: 15920361

GI number: 15920361

Start: 206242

End: 206877

Strand: Direct

Name: pcm [H]

Synonym: ST0182

Alternate gene names: 15920361

Gene position: 206242-206877 (Clockwise)

Preceding gene: 15920360

Following gene: 15920364

Centisome position: 7.65

GC content: 29.72

Gene sequence:

>636_bases
ATGGCTGAAAAAGAAATAATTAATTATATAAGGACTCTAATAACAAGCGAAGAGCTAATAAGGGCATTTACGAAAGTAGA
TAGAAGGAAATTTCTTCCAGATAAATTAAAAGATAGAGCATATTCAATTAAACATATAGATGAACCATTGCAAATAACTA
AAAACTATACAACTACTGCCTTAAGCCTTGGATTGAAAATGCTTGATTTACTTGAGCTAAAAAAGGAAGATAAGGTGTTA
GAAATAGGCACTGGAATTGGTTATTATACAGCATTAATTGCTGAGATTGTTGGAGGAAAAAATGTTTATACAGTCGAGTA
TGATGAAGAGATGTTTAACATAGCAAAACAAAATCTCAAGGAATACAATGTTAACCTTATTTTTGGAGATGGAAGTATTG
GTTATGAAGAAGCAAAACCGTATGATAAAGCTATAATTTGGGCTGCATCGCCAACATTTCCTTGTCTAATTTACAATCAA
ATTAAAGATCAAGGAATAATTATAGTTCCAATAACAGATAAAAAGGATAGACAAGGATTATACAAAATCGTGAAAGTAAA
ATCACCAACAATGATTAGATATTTTGATGTTATCTTTTCTCCATTAAGAGGTATTTGTGGTTATTGGTTAAATTAA

Upstream 100 bases:

>100_bases
TCGATGTAGGACAATGGATGATAGTGAGTTTTAAGGCTGCGCTCAAAGAAGATATACCAGTTATATTTAAAGCTGAAAAT
AACATAGATAAGCTTAAAGA

Downstream 100 bases:

>100_bases
AAAGAATATTTGCTTAAGACTGTAACAACTTTACGTAACTTTTAATTCCTTTTAATCATTTCTCAATATGATTTTTATTA
TGATCATGTTTATATAAGTG

Product: L-isoaspartate O-methyltransferase

Products: NA

Alternate protein names: L-isoaspartyl protein carboxyl methyltransferase; Protein L-isoaspartyl methyltransferase; Protein-beta-aspartate methyltransferase; PIMT [H]

Number of amino acids: Translated: 211; Mature: 210

Protein sequence:

>211_residues
MAEKEIINYIRTLITSEELIRAFTKVDRRKFLPDKLKDRAYSIKHIDEPLQITKNYTTTALSLGLKMLDLLELKKEDKVL
EIGTGIGYYTALIAEIVGGKNVYTVEYDEEMFNIAKQNLKEYNVNLIFGDGSIGYEEAKPYDKAIIWAASPTFPCLIYNQ
IKDQGIIIVPITDKKDRQGLYKIVKVKSPTMIRYFDVIFSPLRGICGYWLN

Sequences:

>Translated_211_residues
MAEKEIINYIRTLITSEELIRAFTKVDRRKFLPDKLKDRAYSIKHIDEPLQITKNYTTTALSLGLKMLDLLELKKEDKVL
EIGTGIGYYTALIAEIVGGKNVYTVEYDEEMFNIAKQNLKEYNVNLIFGDGSIGYEEAKPYDKAIIWAASPTFPCLIYNQ
IKDQGIIIVPITDKKDRQGLYKIVKVKSPTMIRYFDVIFSPLRGICGYWLN
>Mature_210_residues
AEKEIINYIRTLITSEELIRAFTKVDRRKFLPDKLKDRAYSIKHIDEPLQITKNYTTTALSLGLKMLDLLELKKEDKVLE
IGTGIGYYTALIAEIVGGKNVYTVEYDEEMFNIAKQNLKEYNVNLIFGDGSIGYEEAKPYDKAIIWAASPTFPCLIYNQI
KDQGIIIVPITDKKDRQGLYKIVKVKSPTMIRYFDVIFSPLRGICGYWLN

Specific function: Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins [H]

COG id: COG2518

COG function: function code O; Protein-L-isoaspartate carboxylmethyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. L- isoaspartyl/D-aspartyl protein methyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI226530908, Length=217, Percent_Identity=28.5714285714286, Blast_Score=75, Evalue=4e-14,
Organism=Escherichia coli, GI1789100, Length=166, Percent_Identity=31.3253012048193, Blast_Score=91, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI71983477, Length=213, Percent_Identity=27.6995305164319, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI193207222, Length=212, Percent_Identity=27.8301886792453, Blast_Score=69, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000682 [H]

Pfam domain/function: PF01135 PCMT [H]

EC number: =2.1.1.77 [H]

Molecular weight: Translated: 24278; Mature: 24146

Theoretical pI: Translated: 8.54; Mature: 8.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEKEIINYIRTLITSEELIRAFTKVDRRKFLPDKLKDRAYSIKHIDEPLQITKNYTTTA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHCCCHHHH
LSLGLKMLDLLELKKEDKVLEIGTGIGYYTALIAEIVGGKNVYTVEYDEEMFNIAKQNLK
HHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHCCCEEEEEEECHHHHHHHHHHHH
EYNVNLIFGDGSIGYEEAKPYDKAIIWAASPTFPCLIYNQIKDQGIIIVPITDKKDRQGL
HCCCEEEECCCCCCCCCCCCCCCEEEEECCCCCCEEEEECCCCCCEEEEEECCCCCHHHH
YKIVKVKSPTMIRYFDVIFSPLRGICGYWLN
HHHEEECCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AEKEIINYIRTLITSEELIRAFTKVDRRKFLPDKLKDRAYSIKHIDEPLQITKNYTTTA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHCCCHHHH
LSLGLKMLDLLELKKEDKVLEIGTGIGYYTALIAEIVGGKNVYTVEYDEEMFNIAKQNLK
HHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHCCCEEEEEEECHHHHHHHHHHHH
EYNVNLIFGDGSIGYEEAKPYDKAIIWAASPTFPCLIYNQIKDQGIIIVPITDKKDRQGL
HCCCEEEECCCCCCCCCCCCCCCEEEEECCCCCCEEEEECCCCCCEEEEEECCCCCHHHH
YKIVKVKSPTMIRYFDVIFSPLRGICGYWLN
HHHEEECCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA