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The map label for this gene is yjjN [C]

Identifier: 15920254

GI number: 15920254

Start: 97632

End: 98567

Strand: Direct

Name: yjjN [C]

Synonym: ST0075

Alternate gene names: 15920254

Gene position: 97632-98567 (Clockwise)

Preceding gene: 15920253

Following gene: 15920255

Centisome position: 3.62

GC content: 34.72

Gene sequence:

>936_bases
ATGAAGGCGGCTGTTCTCTATAACTTTAATGAGAGTTTTAAAATAGAAGATTCACAACCCAGAGGTGTCGGTGTCAAAGT
AAACGTGGTAGCAACTGGTATATGCGGAAGGGATATTGTTATTTGGAAAGGCGGATTTAGAAACTTAAAAACACCCATAA
TTTTAGGTCATGAAATTGTTGGGTACTACAACGGTAAACCAGTAGCTGTATACCCAAACATATATTGTGGGAAATGTGAA
TATTGTAAAAAGGGAAAAGAAAATCTCTGTGATAATGCTATAATAATAGGGGAAAATCAGAACTACAGCGGGGGTTATGC
AGAAGAAGTTATAGTACCAGAGACTAATTTAATACCTCTTCCAGACGAGAAGTTTGAAAAATATGCAGCTGCACTAGATC
CGGTAGCTACAGCAATTCATGCTACAAAGCTAGTTGAACTAAACGACGAAAGTAAAGTTCTAGTTACTGGTGGTGGAGGA
GGAGTAGGTATTCATTTAATTCAATACCTAAAGTACTTAGGAGTAAAGGAGGTTTATGCGTTAACATCTAAAATAGATAA
AGTCAAAGAATTTACGGAGTATATAATAAATGATGTTAAGGGGCATAAATTTGATGTTGTGTTTGAGCTAGTTGGTGCAA
AGACTATAAATGATTCTTTGAGGGCTCTTAATAAAGAAGGAACTTTAGTACTAATAGGAAATGTTGAAGGAGAACCTATA
ACGTTAAGCAGACCGGCATTATCCATTATGAGACAACACAAGATTATAGGATCAGCTTCTTATACTAAGAAGGAATACGA
AGAAGCAGTTAAGTTAATTCATGAAAACAAGATAGTTCCAATATACAGAGAATATAGCCTTGATGATATAAATCAAGCTT
ACATGGATTTAGTAAATAGGAAGGTTTTTGGAAGAGCTGTACTAAAAATTAGATAA

Upstream 100 bases:

>100_bases
TGCCTACTAAACGTTAAAAGCCATTAGATATGCAACTACTACTGTGAGATTATCAACAGAGTAAGAACATTTTTATACGT
TTCTAAATATAATATAAGTT

Downstream 100 bases:

>100_bases
GATAAGAAAGTTTTTATTTATAAGCTGTTATATGTAATTATGGTCTTGCCTTTTAAGTCAGCAGAAGTCTTCTCTGTGGA
AGTTTCAGAAAAACATGAAT

Product: NAD-dependent alcohol dehydrogenase

Products: NA

Alternate protein names: Acryloyl-CoA reductase [H]

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MKAAVLYNFNESFKIEDSQPRGVGVKVNVVATGICGRDIVIWKGGFRNLKTPIILGHEIVGYYNGKPVAVYPNIYCGKCE
YCKKGKENLCDNAIIIGENQNYSGGYAEEVIVPETNLIPLPDEKFEKYAAALDPVATAIHATKLVELNDESKVLVTGGGG
GVGIHLIQYLKYLGVKEVYALTSKIDKVKEFTEYIINDVKGHKFDVVFELVGAKTINDSLRALNKEGTLVLIGNVEGEPI
TLSRPALSIMRQHKIIGSASYTKKEYEEAVKLIHENKIVPIYREYSLDDINQAYMDLVNRKVFGRAVLKIR

Sequences:

>Translated_311_residues
MKAAVLYNFNESFKIEDSQPRGVGVKVNVVATGICGRDIVIWKGGFRNLKTPIILGHEIVGYYNGKPVAVYPNIYCGKCE
YCKKGKENLCDNAIIIGENQNYSGGYAEEVIVPETNLIPLPDEKFEKYAAALDPVATAIHATKLVELNDESKVLVTGGGG
GVGIHLIQYLKYLGVKEVYALTSKIDKVKEFTEYIINDVKGHKFDVVFELVGAKTINDSLRALNKEGTLVLIGNVEGEPI
TLSRPALSIMRQHKIIGSASYTKKEYEEAVKLIHENKIVPIYREYSLDDINQAYMDLVNRKVFGRAVLKIR
>Mature_311_residues
MKAAVLYNFNESFKIEDSQPRGVGVKVNVVATGICGRDIVIWKGGFRNLKTPIILGHEIVGYYNGKPVAVYPNIYCGKCE
YCKKGKENLCDNAIIIGENQNYSGGYAEEVIVPETNLIPLPDEKFEKYAAALDPVATAIHATKLVELNDESKVLVTGGGG
GVGIHLIQYLKYLGVKEVYALTSKIDKVKEFTEYIINDVKGHKFDVVFELVGAKTINDSLRALNKEGTLVLIGNVEGEPI
TLSRPALSIMRQHKIIGSASYTKKEYEEAVKLIHENKIVPIYREYSLDDINQAYMDLVNRKVFGRAVLKIR

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the acryloyl- CoA dependent NADPH oxidation and formation of propionyl-CoA. Inactive towards 3-hydroxypropionyl-CoA, NADH and crotonyl-CoA [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Escherichia coli, GI226510992, Length=314, Percent_Identity=27.7070063694268, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1790718, Length=303, Percent_Identity=27.0627062706271, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1788073, Length=318, Percent_Identity=26.7295597484277, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1788407, Length=324, Percent_Identity=25.6172839506173, Blast_Score=88, Evalue=6e-19,
Organism=Escherichia coli, GI1790045, Length=238, Percent_Identity=28.5714285714286, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1787863, Length=199, Percent_Identity=30.6532663316583, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI87081918, Length=344, Percent_Identity=27.6162790697674, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1786825, Length=285, Percent_Identity=24.9122807017544, Blast_Score=61, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI71988145, Length=305, Percent_Identity=28.1967213114754, Blast_Score=78, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI17562878, Length=194, Percent_Identity=30.4123711340206, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17562876, Length=272, Percent_Identity=27.9411764705882, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI25146526, Length=370, Percent_Identity=23.2432432432432, Blast_Score=65, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17562584, Length=332, Percent_Identity=26.8072289156627, Blast_Score=65, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6319258, Length=310, Percent_Identity=27.4193548387097, Blast_Score=91, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6322619, Length=312, Percent_Identity=25.9615384615385, Blast_Score=81, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6319955, Length=312, Percent_Identity=25.9615384615385, Blast_Score=80, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6319257, Length=259, Percent_Identity=27.7992277992278, Blast_Score=74, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6319520, Length=218, Percent_Identity=31.1926605504587, Blast_Score=69, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6323961, Length=341, Percent_Identity=21.9941348973607, Blast_Score=63, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6319621, Length=344, Percent_Identity=23.8372093023256, Blast_Score=62, Evalue=9e-11,
Organism=Drosophila melanogaster, GI17137530, Length=223, Percent_Identity=26.0089686098655, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI17737897, Length=200, Percent_Identity=25.5, Blast_Score=65, Evalue=5e-11,
Organism=Drosophila melanogaster, GI17737895, Length=373, Percent_Identity=24.9329758713137, Blast_Score=65, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR002328
- InterPro:   IPR011032
- InterPro:   IPR016040
- InterPro:   IPR002364 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: =1.3.1.84 [H]

Molecular weight: Translated: 34546; Mature: 34546

Theoretical pI: Translated: 8.17; Mature: 8.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAAVLYNFNESFKIEDSQPRGVGVKVNVVATGICGRDIVIWKGGFRNLKTPIILGHEIV
CCEEEEEECCCCEEECCCCCCCCCEEEEEEEECCCCCEEEEEECCCCCCCCCEEECCEEE
GYYNGKPVAVYPNIYCGKCEYCKKGKENLCDNAIIIGENQNYSGGYAEEVIVPETNLIPL
EEECCEEEEECCCCEECCCHHHHCCHHHCCCCEEEEECCCCCCCCCCCEEECCCCCEECC
PDEKFEKYAAALDPVATAIHATKLVELNDESKVLVTGGGGGVGIHLIQYLKYLGVKEVYA
CHHHHHHHHHHHHHHHHHHHHEEEEEECCCCEEEEEECCCCHHHHHHHHHHHHCHHHHHH
LTSKIDKVKEFTEYIINDVKGHKFDVVFELVGAKTINDSLRALNKEGTLVLIGNVEGEPI
HHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCHHHCHHHHHHCCCCCEEEEECCCCCCE
TLSRPALSIMRQHKIIGSASYTKKEYEEAVKLIHENKIVPIYREYSLDDINQAYMDLVNR
EECCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHH
KVFGRAVLKIR
HHHCEEEEEEC
>Mature Secondary Structure
MKAAVLYNFNESFKIEDSQPRGVGVKVNVVATGICGRDIVIWKGGFRNLKTPIILGHEIV
CCEEEEEECCCCEEECCCCCCCCCEEEEEEEECCCCCEEEEEECCCCCCCCCEEECCEEE
GYYNGKPVAVYPNIYCGKCEYCKKGKENLCDNAIIIGENQNYSGGYAEEVIVPETNLIPL
EEECCEEEEECCCCEECCCHHHHCCHHHCCCCEEEEECCCCCCCCCCCEEECCCCCEECC
PDEKFEKYAAALDPVATAIHATKLVELNDESKVLVTGGGGGVGIHLIQYLKYLGVKEVYA
CHHHHHHHHHHHHHHHHHHHHEEEEEECCCCEEEEEECCCCHHHHHHHHHHHHCHHHHHH
LTSKIDKVKEFTEYIINDVKGHKFDVVFELVGAKTINDSLRALNKEGTLVLIGNVEGEPI
HHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCHHHCHHHHHHCCCCCEEEEECCCCCCE
TLSRPALSIMRQHKIIGSASYTKKEYEEAVKLIHENKIVPIYREYSLDDINQAYMDLVNR
EECCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHH
KVFGRAVLKIR
HHHCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11572479 [H]