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The map label for this gene is ptrB [C]

Identifier: 15920171

GI number: 15920171

Start: 3744

End: 5483

Strand: Direct

Name: ptrB [C]

Synonym: ST0002

Alternate gene names: 15920171

Gene position: 3744-5483 (Clockwise)

Preceding gene: 15920169

Following gene: 15920173

Centisome position: 0.14

GC content: 38.33

Gene sequence:

>1740_bases
ATGGACGAATACGAGTATTTAGAAAACTTATCAGACCCCAGAACCATATCCTTTATAGAGAAGGAAAATAAAGAGACGGA
GAGCAAACTGGGGAAGAAAGCTATGGAACTTTATCCCCTTCTCCTCGAAATGGATAAGGAGCCTTACGTTTTAAGTATGT
TCGCTTATGACGAAGAAAACCCGGCAATTTTGCTTTACGGCGAGAAAAGTCAATTATTATTAGGTAATAAGACCATCTAC
ATTCCACCTGAAGGTTATGTAGCCTCAGAAATCTGGAAAGTCTATAACTCAAAGGAGATAGGTGTTAGTATAGAAAGAAA
AGGAAGTGACAAGATCATCACATTGCTCATTTCTCCGGAAGGGAAAATCAGAGAATTAGGCGAGATGGTAGAATCTCCAT
TTTACTTTAAAGGTGAGTTGTGCTATATTAAGAGCTATAGGTATTCCCCACCTCCAGATGGTGGTGATTATCCCGCAGAC
AGAGTGTTTTGTAAAGATGAAATAGTTTACGGCAAAGACATGAAACCCGGTGAGTTTGTAACAATAAAAGTGTTTGATGA
TTTCCTCACTTTAGTTAGGCAAAAGGGTTGGAGGTATGGTGAACTTTATGTAGGAGAGGGATTTGATTCCCTGAGGAAAG
TAGATGAAGGAGAAGTGATAGATGTTATAGATTTTCAACAAGGTGAGGTGATTTATCAGAAGAATAATGCAGTTTACCTC
GGTAACACGAAGGTTGTAGAAGTTGATTATCCAGTTCTAGGTGTTTCTCATATAGGGGATAAAATTGCGGTTGAGGTAAT
AAAAGAGTATAGGACTCCTTTAATTTTTTACGATATTAAAGGAAAGAAAATAGGGGAAGAAGTACACGATAACATAACCT
TCATGGACGGTAAAGGTCACACTTTATTCCTTGTTGAGACTTCATTCAACTATAAATTCAGAGTGGTAAAGAAGAGTGAT
GAAGGGAGAGGGGAAGTGCTAATGCAATACGGAAATTATGATGTGACAGTAAAAGACTTATACGTCAAGGGAGATGTATT
ACTTCACGGGTTTTTAGTCTCTAAAGCCAATAACCCTAAAGGAGTAATAGTTTACGGTTATGGAGGTTTTAGAATTCCCT
TACTTCCCTCTCTTACATCTGTAATGAGGGTATTGTTGAACGAGGGGTATTCGATATTGATAACTAACCTAAGAGGTGGT
TATGAGAACGGGGAGGAGTGGCATAAAGCTGGTATGCTTTTGAATAAGAAGAATGTGTTTAAGGACTTTGCTGAGTTTTT
AAGGCTTGTGAAACTTATGGGTGGAAAAGCGGTAGCAATGGGAGGGAGTAACGGAGGGTTATTAGTAGGTGCTACGATAA
ATGAGTATCCGGAATTAATTGATTGCGCAGTAATAGGCCACCCTGTGCTGGATATGTTGAGGTATGATAAGCTTTATGTT
GGAAAATACTGGGTTGAGGAGTACGGAGATCCTAATGATCCTAAATACACAGAGTATTTGCTATCTTACAGTCCTTACCA
TAACTTGAAAAAAGGTTTACCTAAGACTTTCGTTTATACTGGTATTAATGATGATAGGGTTCATCCGGCACATGCATTGA
AATATGTTGCTAAGTCTAAGAGTTTAGGAAATGATGTAATGCTTTTTGTAAATGATTCCGGTCATTCAATAGCTGATCCA
GAATCTAAAGCGAGAGAGGAGTCATATGTCGTGTCGTTCATAGAAGAGTGTTTGCGTTAA

Upstream 100 bases:

>100_bases
TGCGTATATTGCCCGCCAGATATAAGATGACTATGCTTTCCCAATTCACGCAACTATTCATGTCTTTAATGTTTATTAAC
CTTCTCCCAATTCATTACTT

Downstream 100 bases:

>100_bases
GTACTTAGGGACTTTTAACCTGAGCTAACAATTAGATCAGCTGAGGGGCTTCTCAAACAGTCATGGGATTCGAAAATTAT
TTGCAATATCATATCAAACT

Product: prolyl endopeptidase

Products: Hydrolyzed protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 579; Mature: 579

Protein sequence:

>579_residues
MDEYEYLENLSDPRTISFIEKENKETESKLGKKAMELYPLLLEMDKEPYVLSMFAYDEENPAILLYGEKSQLLLGNKTIY
IPPEGYVASEIWKVYNSKEIGVSIERKGSDKIITLLISPEGKIRELGEMVESPFYFKGELCYIKSYRYSPPPDGGDYPAD
RVFCKDEIVYGKDMKPGEFVTIKVFDDFLTLVRQKGWRYGELYVGEGFDSLRKVDEGEVIDVIDFQQGEVIYQKNNAVYL
GNTKVVEVDYPVLGVSHIGDKIAVEVIKEYRTPLIFYDIKGKKIGEEVHDNITFMDGKGHTLFLVETSFNYKFRVVKKSD
EGRGEVLMQYGNYDVTVKDLYVKGDVLLHGFLVSKANNPKGVIVYGYGGFRIPLLPSLTSVMRVLLNEGYSILITNLRGG
YENGEEWHKAGMLLNKKNVFKDFAEFLRLVKLMGGKAVAMGGSNGGLLVGATINEYPELIDCAVIGHPVLDMLRYDKLYV
GKYWVEEYGDPNDPKYTEYLLSYSPYHNLKKGLPKTFVYTGINDDRVHPAHALKYVAKSKSLGNDVMLFVNDSGHSIADP
ESKAREESYVVSFIEECLR

Sequences:

>Translated_579_residues
MDEYEYLENLSDPRTISFIEKENKETESKLGKKAMELYPLLLEMDKEPYVLSMFAYDEENPAILLYGEKSQLLLGNKTIY
IPPEGYVASEIWKVYNSKEIGVSIERKGSDKIITLLISPEGKIRELGEMVESPFYFKGELCYIKSYRYSPPPDGGDYPAD
RVFCKDEIVYGKDMKPGEFVTIKVFDDFLTLVRQKGWRYGELYVGEGFDSLRKVDEGEVIDVIDFQQGEVIYQKNNAVYL
GNTKVVEVDYPVLGVSHIGDKIAVEVIKEYRTPLIFYDIKGKKIGEEVHDNITFMDGKGHTLFLVETSFNYKFRVVKKSD
EGRGEVLMQYGNYDVTVKDLYVKGDVLLHGFLVSKANNPKGVIVYGYGGFRIPLLPSLTSVMRVLLNEGYSILITNLRGG
YENGEEWHKAGMLLNKKNVFKDFAEFLRLVKLMGGKAVAMGGSNGGLLVGATINEYPELIDCAVIGHPVLDMLRYDKLYV
GKYWVEEYGDPNDPKYTEYLLSYSPYHNLKKGLPKTFVYTGINDDRVHPAHALKYVAKSKSLGNDVMLFVNDSGHSIADP
ESKAREESYVVSFIEECLR
>Mature_579_residues
MDEYEYLENLSDPRTISFIEKENKETESKLGKKAMELYPLLLEMDKEPYVLSMFAYDEENPAILLYGEKSQLLLGNKTIY
IPPEGYVASEIWKVYNSKEIGVSIERKGSDKIITLLISPEGKIRELGEMVESPFYFKGELCYIKSYRYSPPPDGGDYPAD
RVFCKDEIVYGKDMKPGEFVTIKVFDDFLTLVRQKGWRYGELYVGEGFDSLRKVDEGEVIDVIDFQQGEVIYQKNNAVYL
GNTKVVEVDYPVLGVSHIGDKIAVEVIKEYRTPLIFYDIKGKKIGEEVHDNITFMDGKGHTLFLVETSFNYKFRVVKKSD
EGRGEVLMQYGNYDVTVKDLYVKGDVLLHGFLVSKANNPKGVIVYGYGGFRIPLLPSLTSVMRVLLNEGYSILITNLRGG
YENGEEWHKAGMLLNKKNVFKDFAEFLRLVKLMGGKAVAMGGSNGGLLVGATINEYPELIDCAVIGHPVLDMLRYDKLYV
GKYWVEEYGDPNDPKYTEYLLSYSPYHNLKKGLPKTFVYTGINDDRVHPAHALKYVAKSKSLGNDVMLFVNDSGHSIADP
ESKAREESYVVSFIEECLR

Specific function: Cleaves Peptide Bonds On The C-Terminal Side Of Lysyl And Argininyl Residues. [C]

COG id: COG1505

COG function: function code E; Serine proteases of the peptidase family S9A

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S9B family [H]

Homologues:

Organism=Homo sapiens, GI41349456, Length=245, Percent_Identity=37.1428571428571, Blast_Score=151, Evalue=2e-36,
Organism=Homo sapiens, GI108860686, Length=210, Percent_Identity=28.5714285714286, Blast_Score=76, Evalue=7e-14,
Organism=Homo sapiens, GI284172438, Length=190, Percent_Identity=30, Blast_Score=76, Evalue=9e-14,
Organism=Homo sapiens, GI284172431, Length=190, Percent_Identity=30, Blast_Score=76, Evalue=9e-14,
Organism=Homo sapiens, GI108860692, Length=190, Percent_Identity=30, Blast_Score=76, Evalue=1e-13,
Organism=Homo sapiens, GI284172420, Length=190, Percent_Identity=30, Blast_Score=76, Evalue=1e-13,
Organism=Homo sapiens, GI284172413, Length=190, Percent_Identity=30, Blast_Score=76, Evalue=1e-13,
Organism=Homo sapiens, GI70778815, Length=190, Percent_Identity=30, Blast_Score=76, Evalue=1e-13,
Organism=Escherichia coli, GI1788150, Length=224, Percent_Identity=35.7142857142857, Blast_Score=122, Evalue=7e-29,
Organism=Drosophila melanogaster, GI24583414, Length=460, Percent_Identity=32.1739130434783, Blast_Score=163, Evalue=3e-40,
Organism=Drosophila melanogaster, GI221510989, Length=253, Percent_Identity=35.9683794466403, Blast_Score=147, Evalue=2e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001375
- InterPro:   IPR002470
- InterPro:   IPR004106 [H]

Pfam domain/function: PF00326 Peptidase_S9; PF02897 Peptidase_S9_N [H]

EC number: 3.4.21.83 [C]

Molecular weight: Translated: 65775; Mature: 65775

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDEYEYLENLSDPRTISFIEKENKETESKLGKKAMELYPLLLEMDKEPYVLSMFAYDEEN
CCHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCC
PAILLYGEKSQLLLGNKTIYIPPEGYVASEIWKVYNSKEIGVSIERKGSDKIITLLISPE
CEEEEECCCCCEEECCEEEEECCCCCHHHHHHHHHCCCCCCEEEEECCCCEEEEEEECCC
GKIRELGEMVESPFYFKGELCYIKSYRYSPPPDGGDYPADRVFCKDEIVYGKDMKPGEFV
CHHHHHHHHHCCCCEEECEEEEEEECCCCCCCCCCCCCCCCEEECCCEEECCCCCCCCEE
TIKVFDDFLTLVRQKGWRYGELYVGEGFDSLRKVDEGEVIDVIDFQQGEVIYQKNNAVYL
EEEEHHHHHHHHHHCCCCEEEEEEECCHHHHHCCCCCCEEEEEECCCCCEEEEECCEEEE
GNTKVVEVDYPVLGVSHIGDKIAVEVIKEYRTPLIFYDIKGKKIGEEVHDNITFMDGKGH
CCEEEEEECCCEEEHHHCCHHHHHHHHHHHCCCEEEEEECCCHHHHHHHCCEEEECCCCC
TLFLVETSFNYKFRVVKKSDEGRGEVLMQYGNYDVTVKDLYVKGDVLLHGFLVSKANNPK
EEEEEEECCCEEEEEEEECCCCCCEEEEEECCEEEEEEEEEEECCEEEEEEEEECCCCCC
GVIVYGYGGFRIPLLPSLTSVMRVLLNEGYSILITNLRGGYENGEEWHKAGMLLNKKNVF
EEEEECCCCEEECCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHCCEECHHHHH
KDFAEFLRLVKLMGGKAVAMGGSNGGLLVGATINEYPELIDCAVIGHPVLDMLRYDKLYV
HHHHHHHHHHHHHCCCEEEEECCCCCEEEECCHHHCHHHHHHHHHCHHHHHHHHHCEEEE
GKYWVEEYGDPNDPKYTEYLLSYSPYHNLKKGLPKTFVYTGINDDRVHPAHALKYVAKSK
EEHHHHHCCCCCCCCHHHHHCCCCCHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHC
SLGNDVMLFVNDSGHSIADPESKAREESYVVSFIEECLR
CCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MDEYEYLENLSDPRTISFIEKENKETESKLGKKAMELYPLLLEMDKEPYVLSMFAYDEEN
CCHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCC
PAILLYGEKSQLLLGNKTIYIPPEGYVASEIWKVYNSKEIGVSIERKGSDKIITLLISPE
CEEEEECCCCCEEECCEEEEECCCCCHHHHHHHHHCCCCCCEEEEECCCCEEEEEEECCC
GKIRELGEMVESPFYFKGELCYIKSYRYSPPPDGGDYPADRVFCKDEIVYGKDMKPGEFV
CHHHHHHHHHCCCCEEECEEEEEEECCCCCCCCCCCCCCCCEEECCCEEECCCCCCCCEE
TIKVFDDFLTLVRQKGWRYGELYVGEGFDSLRKVDEGEVIDVIDFQQGEVIYQKNNAVYL
EEEEHHHHHHHHHHCCCCEEEEEEECCHHHHHCCCCCCEEEEEECCCCCEEEEECCEEEE
GNTKVVEVDYPVLGVSHIGDKIAVEVIKEYRTPLIFYDIKGKKIGEEVHDNITFMDGKGH
CCEEEEEECCCEEEHHHCCHHHHHHHHHHHCCCEEEEEECCCHHHHHHHCCEEEECCCCC
TLFLVETSFNYKFRVVKKSDEGRGEVLMQYGNYDVTVKDLYVKGDVLLHGFLVSKANNPK
EEEEEEECCCEEEEEEEECCCCCCEEEEEECCEEEEEEEEEEECCEEEEEEEEECCCCCC
GVIVYGYGGFRIPLLPSLTSVMRVLLNEGYSILITNLRGGYENGEEWHKAGMLLNKKNVF
EEEEECCCCEEECCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHCCEECHHHHH
KDFAEFLRLVKLMGGKAVAMGGSNGGLLVGATINEYPELIDCAVIGHPVLDMLRYDKLYV
HHHHHHHHHHHHHCCCEEEEECCCCCEEEECCHHHCHHHHHHHHHCHHHHHHHHHCEEEE
GKYWVEEYGDPNDPKYTEYLLSYSPYHNLKKGLPKTFVYTGINDDRVHPAHALKYVAKSK
EEHHHHHCCCCCCCCHHHHHCCCCCHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHC
SLGNDVMLFVNDSGHSIADPESKAREESYVVSFIEECLR
CCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Ca2+ [C]

Kcat value (1/min): 11820 [C]

Specific activity: NA

Km value (mM): 0.6 {N-benzoyl-Arg} 0.92 {benzoyl-Lys} 0.23 {tosyl-Arg} 0.5 {benzoyl-Arg} 0.48 {benzoyl-Arg} 0.25 {benzoyl-Arg} 0.33 {N-benzyloxycarbonyl-Lys} 0.31 {N-benzyloxycarbonyl-Lys} 0.47 {tosyl-Lys-methyl} 80 {acetyl-tyrosine} [C]

Substrates: Protein; H2O [C]

Specific reaction: Protein + H2O = hydrolyzed protein [C]

General reaction: Peptide bond hydrolysis [C]

Inhibitor: Antipain; Aromaticamidines; Benzamidine; Co2+; DFP; Fe2+; Hg2+; L-Arginine; Leupeptin sulfhydryl agents, trypsin inhibitors, 1, 10-phenanthroline; p-Aminobenzamidine; Tosyl -Leuchloromethyl ketone; Zn2+ [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]