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The map label for this gene is kefB [H]

Identifier: 15893175

GI number: 15893175

Start: 1157229

End: 1158965

Strand: Reverse

Name: kefB [H]

Synonym: RC1252

Alternate gene names: 15893175

Gene position: 1158965-1157229 (Counterclockwise)

Preceding gene: 15893176

Following gene: 15893174

Centisome position: 91.35

GC content: 35.58

Gene sequence:

>1737_bases
TTGGAAAAGATGAACGATCATATTCTTATTAACGTTATAATTTTACTCGGTACTGCGGTTTTTATCGTGGCTATACTTAA
GCGTCTTAGATTAAGTCCGGTACTTGGTTACTTAATTGCCGGAGCTGCAATAGGTGATCACGGTTTAAAAATCGTAACAT
ATGACCAAACTAAGTTACTAGGTGAACTTGGTGTAGTATTTCTATTATTTGCGATAGGACTTGAGTTATCCTTCGAGCGA
TTAAAAGCTATGAGACGATATGTATTTGGTCTCGGTAGTTTGCAAGTTTTAACAACCGCTATAGTAATTGCCGGTGCAAT
GGTGCTTATAGACGGCAATAGTAGTGCGGCTATTATTACCGGCGGTGGTCTGGCCCTTTCATCTACAGCACTTGTTATGC
AGGTTATTGAGGAAAATCGTAGTCAATCAACGCAAATAGGAAGAGTTTCTTTAGCTATTCTGTTACTACAAGATTTGGCC
GTAGTGCCATTGCTTGTGATAGTTCCGTTACTTGCCGGTAACAATAAAGCCTCGCTTGCAGCGGCTCTTGGTATTGCACT
CTTAAAAGCCGTAACGGCCTTACTTACAATATTTATTGTCGGTCGTGTTTTTCTTCGTCCTGTATTTTCATTCATTTCAT
CAGAAAGTAATAATGCAAGTGAGTTACCTATTTCGATGACTTTGTTAATAGTACTTTCTGCTGCTTGGGCTACCGAAACA
TTTGGTTTATCTTTAGCTCTAGGAGCATTTGTTGCCGGAGTATTAGTTGCAGAAACTGAATTTAGATTGCAGGCAGAGGA
AAGTATTTATCCTTTCAAAAGTTTATTTTTAGGATTGTTTTTCATGACCGTGGGTATGAATATCGATGCCCTAGAAATGT
ATGAAAAAATATCTCATATTCTTACTTTATCTATTGCTTTAATAGGTATAAAAACCTTAATCATAACGGCTTTTTGTATT
TTATTCGGTTTTAATAAAGGAGTTGCTTTTTATTCAGGCTTATTATTATCGCAAGGTGGAGAGTTTGGTTTTATATTATT
CAGTTTAGGTAAAGATAGTGGAGTATTAGAAGAAAGTACTGCCGATATATTATTACTCGTAGTTACTTTTACTATGGCAC
TTACTCCATTACTCGCAGCTTTAGGGCAAAAGATTGCTGAGAAAGTTGATAAAGGACTTGGTAAAACTCCGACTCAAATG
ATTGAGCTTGGGGCAAGAGATTTAACAAACCATATTATTATTGCCGGACTCGGTAATACCGGTAAAATGGTAGCAAGAGT
TTTGGAAGCGGAAGGTATAAGTTACGTAATACTTGATTTAGATGATGATAGAGTAAAAGAAGAGCTATCAAACGGTTTGC
CGGTTTTTAAAGGTGATGTATCGCAAGCCGATACTCTAAAGGCATTAGGTACGGAAAGAGCATTCGCTATAATACTTACT
ATGAATAATCAAGTAACTATCAAGAAATCGCTTAAAACAATTAGTGGTAATTATCAGGATATACCGGTTGTCGTTAAGTT
AAAAAATTTAAAAAATGCTAGAGAGTTTTATGATTTAGGTGCCACAACTATTATTCCAGAAAGTTATGAGACTGGATTAC
AAATAGGTGGAACAGTCCTAAAAAATATAGGTATTAGTGAGCAGGAAATTAATAGAATAAAAGTACAATTTAGGCTTGGT
AATTATATAATAGCAAAAAAAGAGGATGCTTTATCTGAAGTGGAAGATAATGATTAA

Upstream 100 bases:

>100_bases
AGGGGCTTGGTAAATCAGAATGGGTACTAATAGATGCAGGTACTATTTTAATTAATATTTTTTATCCTGAAGTACGGGAG
CATTTTAAGTTAGAAGAAAT

Downstream 100 bases:

>100_bases
AATTCTTATTACTTTAATTATTGTAATATTATCAACAACAATTAATGCCGATAATAAAAAATTGCCTATTCCGAGATTTG
TTTCAATAAAATCTAATGAA

Product: glutathione-regulated potassium-efflux system protein kefB

Products: Proton [Cytoplasm]; K (I) [Periplasm] [C]

Alternate protein names: K(+)/H(+) antiporter; NEM-activatable K(+)/H(+) antiporter [H]

Number of amino acids: Translated: 578; Mature: 578

Protein sequence:

>578_residues
MEKMNDHILINVIILLGTAVFIVAILKRLRLSPVLGYLIAGAAIGDHGLKIVTYDQTKLLGELGVVFLLFAIGLELSFER
LKAMRRYVFGLGSLQVLTTAIVIAGAMVLIDGNSSAAIITGGGLALSSTALVMQVIEENRSQSTQIGRVSLAILLLQDLA
VVPLLVIVPLLAGNNKASLAAALGIALLKAVTALLTIFIVGRVFLRPVFSFISSESNNASELPISMTLLIVLSAAWATET
FGLSLALGAFVAGVLVAETEFRLQAEESIYPFKSLFLGLFFMTVGMNIDALEMYEKISHILTLSIALIGIKTLIITAFCI
LFGFNKGVAFYSGLLLSQGGEFGFILFSLGKDSGVLEESTADILLLVVTFTMALTPLLAALGQKIAEKVDKGLGKTPTQM
IELGARDLTNHIIIAGLGNTGKMVARVLEAEGISYVILDLDDDRVKEELSNGLPVFKGDVSQADTLKALGTERAFAIILT
MNNQVTIKKSLKTISGNYQDIPVVVKLKNLKNAREFYDLGATTIIPESYETGLQIGGTVLKNIGISEQEINRIKVQFRLG
NYIIAKKEDALSEVEDND

Sequences:

>Translated_578_residues
MEKMNDHILINVIILLGTAVFIVAILKRLRLSPVLGYLIAGAAIGDHGLKIVTYDQTKLLGELGVVFLLFAIGLELSFER
LKAMRRYVFGLGSLQVLTTAIVIAGAMVLIDGNSSAAIITGGGLALSSTALVMQVIEENRSQSTQIGRVSLAILLLQDLA
VVPLLVIVPLLAGNNKASLAAALGIALLKAVTALLTIFIVGRVFLRPVFSFISSESNNASELPISMTLLIVLSAAWATET
FGLSLALGAFVAGVLVAETEFRLQAEESIYPFKSLFLGLFFMTVGMNIDALEMYEKISHILTLSIALIGIKTLIITAFCI
LFGFNKGVAFYSGLLLSQGGEFGFILFSLGKDSGVLEESTADILLLVVTFTMALTPLLAALGQKIAEKVDKGLGKTPTQM
IELGARDLTNHIIIAGLGNTGKMVARVLEAEGISYVILDLDDDRVKEELSNGLPVFKGDVSQADTLKALGTERAFAIILT
MNNQVTIKKSLKTISGNYQDIPVVVKLKNLKNAREFYDLGATTIIPESYETGLQIGGTVLKNIGISEQEINRIKVQFRLG
NYIIAKKEDALSEVEDND
>Mature_578_residues
MEKMNDHILINVIILLGTAVFIVAILKRLRLSPVLGYLIAGAAIGDHGLKIVTYDQTKLLGELGVVFLLFAIGLELSFER
LKAMRRYVFGLGSLQVLTTAIVIAGAMVLIDGNSSAAIITGGGLALSSTALVMQVIEENRSQSTQIGRVSLAILLLQDLA
VVPLLVIVPLLAGNNKASLAAALGIALLKAVTALLTIFIVGRVFLRPVFSFISSESNNASELPISMTLLIVLSAAWATET
FGLSLALGAFVAGVLVAETEFRLQAEESIYPFKSLFLGLFFMTVGMNIDALEMYEKISHILTLSIALIGIKTLIITAFCI
LFGFNKGVAFYSGLLLSQGGEFGFILFSLGKDSGVLEESTADILLLVVTFTMALTPLLAALGQKIAEKVDKGLGKTPTQM
IELGARDLTNHIIIAGLGNTGKMVARVLEAEGISYVILDLDDDRVKEELSNGLPVFKGDVSQADTLKALGTERAFAIILT
MNNQVTIKKSLKTISGNYQDIPVVVKLKNLKNAREFYDLGATTIIPESYETGLQIGGTVLKNIGISEQEINRIKVQFRLG
NYIIAKKEDALSEVEDND

Specific function: Transport system that facilitates potassium-efflux, possibly by potassium-proton antiport [H]

COG id: COG0475

COG function: function code P; Kef-type K+ transport systems, membrane components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RCK N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI157388921, Length=377, Percent_Identity=25.9946949602122, Blast_Score=79, Evalue=1e-14,
Organism=Escherichia coli, GI1789749, Length=563, Percent_Identity=35.5239786856128, Blast_Score=287, Evalue=2e-78,
Organism=Escherichia coli, GI1786232, Length=559, Percent_Identity=33.2737030411449, Blast_Score=283, Evalue=2e-77,
Organism=Escherichia coli, GI1786685, Length=514, Percent_Identity=29.5719844357977, Blast_Score=155, Evalue=5e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006153
- InterPro:   IPR020884
- InterPro:   IPR004771
- InterPro:   IPR016040
- InterPro:   IPR003148 [H]

Pfam domain/function: PF00999 Na_H_Exchanger; PF02254 TrkA_N [H]

EC number: NA

Molecular weight: Translated: 62028; Mature: 62028

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKMNDHILINVIILLGTAVFIVAILKRLRLSPVLGYLIAGAAIGDHGLKIVTYDQTKLL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECHHHHH
GELGVVFLLFAIGLELSFERLKAMRRYVFGLGSLQVLTTAIVIAGAMVLIDGNSSAAIIT
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCEEEEEECCCCEEEEE
GGGLALSSTALVMQVIEENRSQSTQIGRVSLAILLLQDLAVVPLLVIVPLLAGNNKASLA
CCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
AALGIALLKAVTALLTIFIVGRVFLRPVFSFISSESNNASELPISMTLLIVLSAAWATET
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHH
FGLSLALGAFVAGVLVAETEFRLQAEESIYPFKSLFLGLFFMTVGMNIDALEMYEKISHI
HHHHHHHHHHHHHHHHHCHHHHEECCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
LTLSIALIGIKTLIITAFCILFGFNKGVAFYSGLLLSQGGEFGFILFSLGKDSGVLEEST
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCH
ADILLLVVTFTMALTPLLAALGQKIAEKVDKGLGKTPTQMIELGARDLTNHIIIAGLGNT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCHHHCCCCEEEEECCCH
GKMVARVLEAEGISYVILDLDDDRVKEELSNGLPVFKGDVSQADTLKALGTERAFAIILT
HHHHHHHHHHCCCEEEEEECCHHHHHHHHHCCCCEEECCCCHHHHHHHHCCCCEEEEEEE
MNNQVTIKKSLKTISGNYQDIPVVVKLKNLKNAREFYDLGATTIIPESYETGLQIGGTVL
ECCCEEEHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHCCCCEECCCCHHCCHHHHHHHH
KNIGISEQEINRIKVQFRLGNYIIAKKEDALSEVEDND
HHCCCCHHHHCEEEEEEEECCEEEEECHHHHHHCCCCC
>Mature Secondary Structure
MEKMNDHILINVIILLGTAVFIVAILKRLRLSPVLGYLIAGAAIGDHGLKIVTYDQTKLL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECHHHHH
GELGVVFLLFAIGLELSFERLKAMRRYVFGLGSLQVLTTAIVIAGAMVLIDGNSSAAIIT
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCEEEEEECCCCEEEEE
GGGLALSSTALVMQVIEENRSQSTQIGRVSLAILLLQDLAVVPLLVIVPLLAGNNKASLA
CCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
AALGIALLKAVTALLTIFIVGRVFLRPVFSFISSESNNASELPISMTLLIVLSAAWATET
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHH
FGLSLALGAFVAGVLVAETEFRLQAEESIYPFKSLFLGLFFMTVGMNIDALEMYEKISHI
HHHHHHHHHHHHHHHHHCHHHHEECCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
LTLSIALIGIKTLIITAFCILFGFNKGVAFYSGLLLSQGGEFGFILFSLGKDSGVLEEST
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCH
ADILLLVVTFTMALTPLLAALGQKIAEKVDKGLGKTPTQMIELGARDLTNHIIIAGLGNT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCHHHCCCCEEEEECCCH
GKMVARVLEAEGISYVILDLDDDRVKEELSNGLPVFKGDVSQADTLKALGTERAFAIILT
HHHHHHHHHHCCCEEEEEECCHHHHHHHHHCCCCEEECCCCHHHHHHHHCCCCEEEEEEE
MNNQVTIKKSLKTISGNYQDIPVVVKLKNLKNAREFYDLGATTIIPESYETGLQIGGTVL
ECCCEEEHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHCCCCEECCCCHHCCHHHHHHHH
KNIGISEQEINRIKVQFRLGNYIIAKKEDALSEVEDND
HHCCCCHHHHCEEEEEEEECCEEEEECHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; K (I) [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + K (I) [Cytoplasm] = Proton [Cytoplasm] + K (I) [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA