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The map label for this gene is lipA [H]

Identifier: 15893068

GI number: 15893068

Start: 1055940

End: 1056848

Strand: Reverse

Name: lipA [H]

Synonym: RC1145

Alternate gene names: 15893068

Gene position: 1056848-1055940 (Counterclockwise)

Preceding gene: 15893069

Following gene: 15893067

Centisome position: 83.3

GC content: 36.74

Gene sequence:

>909_bases
TTGAATTATTTCCGTTTTATGGCTAATTTAAATAAACGACCTGATTGGATAAAAGTAAAAGCTCCTAATTCTACAGAGTA
CTATAATACAAAAGATTTAATAAAGAATCTGAGATTAAATACTGTGTGCGAGGAAGCTGCTTGTCCTAATATTGGTGAGT
GCTGGTCAAAAAAACATACAACTGTGATGATTTTAGGTTCGGTTTGTACTAGAGCTTGTAGGTTTTGTAACGTTAAAACG
GGTAGACCGGATTTACTTGATCCGTATGAACCGCAAAGGTTAGCGGAGGCGGTACAAAAGCTAAATCTTAAGCATGTAGT
AATTACTTCCGTCGATCGTGATGATCTTGAGGATGGCGGAGCGTCACATTTTGCGGAGTGTATTAGCGAAATCCGAAAAT
CATCGCCAAATACTACTATTGAGATTCTAACACCCGATTTTTTAAGAAAAGAAGGAGCAGCTGAAATAATAGCAAACGCA
AAACCTGACGTTTTTAACCATAATGTCGAAACAGTACCGTCTTTATACAAAACGATTAGACCGGGGGCTAGATATTATAA
TTCTTTAAGTTTACTGCATAATATTAAAAAATTATCTCCTGAAATTTTTACAAAATCCGGTATGATGGTAGGACTTGGGG
AAGAAATAAATGAAGTAGTACAGGTTATAGATGATTTAAGAGAAGCAAAGGTTGATTTCCTAACTATCGGGCAGTATCTG
CAGCCTACCAAAAATCATGCTGAGGTCGCAAAATATGTTACGCCGGAAGAGTTTAAATATCTAGAGCGAGTAGCAAAAAC
AAAAGGATTCTTGATGGTTTCGGCAAGCCCGCTGACCCGTTCTTCATACCATGCTGATGAGGATTTTCAAAAATTAAAAG
AGAATTACCAACAAAAACTGGTGTCTTAA

Upstream 100 bases:

>100_bases
TTAGTCGGTCATATGGTAGCAGATATTTTAGACGGCTTAAAAAATAATGAAGATAATAGCGCTCTTGAGCAGAAAGTATT
AAATGAAGTGACAAAATTAA

Downstream 100 bases:

>100_bases
AATATCATGCTTATTGCCTGTGTGGTCAGTTTTCATAGCTATTATCTTTGTTTACTAAAGTAAATAATCCTTATGTTGAT
TAATTTATATATATGATATC

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA [H]

Number of amino acids: Translated: 302; Mature: 302

Protein sequence:

>302_residues
MNYFRFMANLNKRPDWIKVKAPNSTEYYNTKDLIKNLRLNTVCEEAACPNIGECWSKKHTTVMILGSVCTRACRFCNVKT
GRPDLLDPYEPQRLAEAVQKLNLKHVVITSVDRDDLEDGGASHFAECISEIRKSSPNTTIEILTPDFLRKEGAAEIIANA
KPDVFNHNVETVPSLYKTIRPGARYYNSLSLLHNIKKLSPEIFTKSGMMVGLGEEINEVVQVIDDLREAKVDFLTIGQYL
QPTKNHAEVAKYVTPEEFKYLERVAKTKGFLMVSASPLTRSSYHADEDFQKLKENYQQKLVS

Sequences:

>Translated_302_residues
MNYFRFMANLNKRPDWIKVKAPNSTEYYNTKDLIKNLRLNTVCEEAACPNIGECWSKKHTTVMILGSVCTRACRFCNVKT
GRPDLLDPYEPQRLAEAVQKLNLKHVVITSVDRDDLEDGGASHFAECISEIRKSSPNTTIEILTPDFLRKEGAAEIIANA
KPDVFNHNVETVPSLYKTIRPGARYYNSLSLLHNIKKLSPEIFTKSGMMVGLGEEINEVVQVIDDLREAKVDFLTIGQYL
QPTKNHAEVAKYVTPEEFKYLERVAKTKGFLMVSASPLTRSSYHADEDFQKLKENYQQKLVS
>Mature_302_residues
MNYFRFMANLNKRPDWIKVKAPNSTEYYNTKDLIKNLRLNTVCEEAACPNIGECWSKKHTTVMILGSVCTRACRFCNVKT
GRPDLLDPYEPQRLAEAVQKLNLKHVVITSVDRDDLEDGGASHFAECISEIRKSSPNTTIEILTPDFLRKEGAAEIIANA
KPDVFNHNVETVPSLYKTIRPGARYYNSLSLLHNIKKLSPEIFTKSGMMVGLGEEINEVVQVIDDLREAKVDFLTIGQYL
QPTKNHAEVAKYVTPEEFKYLERVAKTKGFLMVSASPLTRSSYHADEDFQKLKENYQQKLVS

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives [H]

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family [H]

Homologues:

Organism=Homo sapiens, GI37577166, Length=285, Percent_Identity=47.0175438596491, Blast_Score=271, Evalue=4e-73,
Organism=Homo sapiens, GI37577164, Length=243, Percent_Identity=46.5020576131687, Blast_Score=229, Evalue=2e-60,
Organism=Escherichia coli, GI1786846, Length=279, Percent_Identity=50.179211469534, Blast_Score=295, Evalue=2e-81,
Organism=Caenorhabditis elegans, GI32564533, Length=258, Percent_Identity=45.7364341085271, Blast_Score=238, Evalue=2e-63,
Organism=Saccharomyces cerevisiae, GI6324770, Length=285, Percent_Identity=47.719298245614, Blast_Score=274, Evalue=1e-74,
Organism=Drosophila melanogaster, GI221513272, Length=283, Percent_Identity=45.9363957597173, Blast_Score=258, Evalue=4e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.8.1.8 [H]

Molecular weight: Translated: 34342; Mature: 34342

Theoretical pI: Translated: 8.05; Mature: 8.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYFRFMANLNKRPDWIKVKAPNSTEYYNTKDLIKNLRLNTVCEEAACPNIGECWSKKHT
CCHHHHHHCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCC
TVMILGSVCTRACRFCNVKTGRPDLLDPYEPQRLAEAVQKLNLKHVVITSVDRDDLEDGG
EEEEHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCHHHCCCCC
ASHFAECISEIRKSSPNTTIEILTPDFLRKEGAAEIIANAKPDVFNHNVETVPSLYKTIR
HHHHHHHHHHHHHCCCCCEEEEECHHHHHHCCHHHHHHCCCCCHHCCCHHHHHHHHHHHC
PGARYYNSLSLLHNIKKLSPEIFTKSGMMVGLGEEINEVVQVIDDLREAKVDFLTIGQYL
CHHHHHHHHHHHHHHHHHCHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHH
QPTKNHAEVAKYVTPEEFKYLERVAKTKGFLMVSASPLTRSSYHADEDFQKLKENYQQKL
CCCCCHHHHHHHCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHH
VS
CC
>Mature Secondary Structure
MNYFRFMANLNKRPDWIKVKAPNSTEYYNTKDLIKNLRLNTVCEEAACPNIGECWSKKHT
CCHHHHHHCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCC
TVMILGSVCTRACRFCNVKTGRPDLLDPYEPQRLAEAVQKLNLKHVVITSVDRDDLEDGG
EEEEHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCHHHCCCCC
ASHFAECISEIRKSSPNTTIEILTPDFLRKEGAAEIIANAKPDVFNHNVETVPSLYKTIR
HHHHHHHHHHHHHCCCCCEEEEECHHHHHHCCHHHHHHCCCCCHHCCCHHHHHHHHHHHC
PGARYYNSLSLLHNIKKLSPEIFTKSGMMVGLGEEINEVVQVIDDLREAKVDFLTIGQYL
CHHHHHHHHHHHHHHHHHCHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHH
QPTKNHAEVAKYVTPEEFKYLERVAKTKGFLMVSASPLTRSSYHADEDFQKLKENYQQKL
CCCCCHHHHHHHCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHH
VS
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA