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The map label for this gene is exsB [H]

Identifier: 15892993

GI number: 15892993

Start: 991273

End: 991959

Strand: Reverse

Name: exsB [H]

Synonym: RC1070

Alternate gene names: 15892993

Gene position: 991959-991273 (Counterclockwise)

Preceding gene: 15892994

Following gene: 15892992

Centisome position: 78.18

GC content: 32.31

Gene sequence:

>687_bases
ATGAAAAAAAAAGCAGTTATTTTATTAAGCGGTGGACCTGATTCTACTACGGTTCTTGAGATAGTGAGCAAAACGGATTA
TGAAATTTATGCTCTAAGTTTTAATTATCATAGACGCAATAGTCTGGAAGTCCAAAAAATCCAAGGATTAATTAAAGATT
ATAATGTAAAACAACATAGGGTTATTAATATTGATTTGCAGTCATTTATCGGTTCTGCATTGACTGATGATAATATAGAT
GTACCAAAATTTCAAAACACAGATCAATTACCAAGTGACATACCGGTTACTTATGTACCGGCACGTAATACAATATTTTT
AAGCTACGCACTAGGAGTTGCTGAGGTTATAGGAGCTAGAGATATTTTTATTGGGGTACACACTAATGATTATACTAACT
ATCCTGATTGTCGTCCTGAATATATAAAATCTTTTGAAGCAATGGCAAATTTAGCAACTAGGGTAGGAGTAAATGGAGAA
AAAATTACTATCCATGCTCCTTTAATAAATATGACCAAAGAGCAAATAATTAAAAAAGGGCTTGAGCTTGGAGTAGATTA
TAGTAAAACAATATCATGTTATGATCCAACAGAAGACGGTTTATCTTGTGGTCAATGTCTATCATGCATTGCACGACTTG
ATGCTTTTAAAAAGAATAATGTACAAGACCCGATTAAGTATGTATAA

Upstream 100 bases:

>100_bases
TAGATAAACGTGTGCATCGAGCTTTAGTACTTGAAGCACTTAGAGCAAAAATTAGTTTAAATGCTTTAGTATCTCAAAAA
TTAACTTTATCGGTAAAACA

Downstream 100 bases:

>100_bases
TTATAGATATATAAAAATTAATAATAAAATAATATGAAGTAAATTTGTATACAAGGTGGAAACCATTGTTCACCTGGTAT
ACTCATTATTTAAAAATCTT

Product: trans-regulatory protein ExsB

Products: NA

Alternate protein names: 7-cyano-7-carbaguanine synthase; PreQ(0) synthase; Queuosine biosynthesis protein queC [H]

Number of amino acids: Translated: 228; Mature: 228

Protein sequence:

>228_residues
MKKKAVILLSGGPDSTTVLEIVSKTDYEIYALSFNYHRRNSLEVQKIQGLIKDYNVKQHRVINIDLQSFIGSALTDDNID
VPKFQNTDQLPSDIPVTYVPARNTIFLSYALGVAEVIGARDIFIGVHTNDYTNYPDCRPEYIKSFEAMANLATRVGVNGE
KITIHAPLINMTKEQIIKKGLELGVDYSKTISCYDPTEDGLSCGQCLSCIARLDAFKKNNVQDPIKYV

Sequences:

>Translated_228_residues
MKKKAVILLSGGPDSTTVLEIVSKTDYEIYALSFNYHRRNSLEVQKIQGLIKDYNVKQHRVINIDLQSFIGSALTDDNID
VPKFQNTDQLPSDIPVTYVPARNTIFLSYALGVAEVIGARDIFIGVHTNDYTNYPDCRPEYIKSFEAMANLATRVGVNGE
KITIHAPLINMTKEQIIKKGLELGVDYSKTISCYDPTEDGLSCGQCLSCIARLDAFKKNNVQDPIKYV
>Mature_228_residues
MKKKAVILLSGGPDSTTVLEIVSKTDYEIYALSFNYHRRNSLEVQKIQGLIKDYNVKQHRVINIDLQSFIGSALTDDNID
VPKFQNTDQLPSDIPVTYVPARNTIFLSYALGVAEVIGARDIFIGVHTNDYTNYPDCRPEYIKSFEAMANLATRVGVNGE
KITIHAPLINMTKEQIIKKGLELGVDYSKTISCYDPTEDGLSCGQCLSCIARLDAFKKNNVQDPIKYV

Specific function: Catalyzes the ATP-dependent conversion of 7-carboxy-7- deazaguanine (CDG) to 7-cyano-7-deazaguanine (preQ(0)) [H]

COG id: COG0603

COG function: function code R; Predicted PP-loop superfamily ATPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queC family [H]

Homologues:

Organism=Escherichia coli, GI1786648, Length=218, Percent_Identity=30.2752293577982, Blast_Score=105, Evalue=3e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018317
- InterPro:   IPR014729 [H]

Pfam domain/function: PF06508 ExsB [H]

EC number: NA

Molecular weight: Translated: 25487; Mature: 25487

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKAVILLSGGPDSTTVLEIVSKTDYEIYALSFNYHRRNSLEVQKIQGLIKDYNVKQHR
CCCEEEEEEECCCCCHHHHEEECCCCEEEEEEEECCCCCCCCHHHHHHHHHHCCCCCCEE
VINIDLQSFIGSALTDDNIDVPKFQNTDQLPSDIPVTYVPARNTIFLSYALGVAEVIGAR
EEEEEHHHHHCHHCCCCCCCCCCCCCCCCCCCCCCEEEECCCCEEEEEHHHHHHHHHCCC
DIFIGVHTNDYTNYPDCRPEYIKSFEAMANLATRVGVNGEKITIHAPLINMTKEQIIKKG
EEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHH
LELGVDYSKTISCYDPTEDGLSCGQCLSCIARLDAFKKNNVQDPIKYV
HHHCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHCC
>Mature Secondary Structure
MKKKAVILLSGGPDSTTVLEIVSKTDYEIYALSFNYHRRNSLEVQKIQGLIKDYNVKQHR
CCCEEEEEEECCCCCHHHHEEECCCCEEEEEEEECCCCCCCCHHHHHHHHHHCCCCCCEE
VINIDLQSFIGSALTDDNIDVPKFQNTDQLPSDIPVTYVPARNTIFLSYALGVAEVIGAR
EEEEEHHHHHCHHCCCCCCCCCCCCCCCCCCCCCCEEEECCCCEEEEEHHHHHHHHHCCC
DIFIGVHTNDYTNYPDCRPEYIKSFEAMANLATRVGVNGEKITIHAPLINMTKEQIIKKG
EEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHH
LELGVDYSKTISCYDPTEDGLSCGQCLSCIARLDAFKKNNVQDPIKYV
HHHCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA