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The map label for this gene is ksgA [H]

Identifier: 15892945

GI number: 15892945

Start: 948447

End: 949394

Strand: Reverse

Name: ksgA [H]

Synonym: RC1022

Alternate gene names: 15892945

Gene position: 949394-948447 (Counterclockwise)

Preceding gene: 15892946

Following gene: 15892944

Centisome position: 74.83

GC content: 35.86

Gene sequence:

>948_bases
ATGTTTTACTGGATTCCCGCTTTCACGGGAATGACATACCCTATGCTCCCTTCAATTGCAAAACACGCAGCTTTACATCA
AGTTAATCCTCTTAAAAAGCACGGACAAAATTTTATTTTTGACAGTAGTCTATGTGATAAAATCGTACGTGCAAGTAACC
TGGCAGAAAATAGCCGAGTATTAGAAATAGGGCCCGGCACAGGAGGGTTAACTAGGTCAATATTGCAGAAAAATCCTGAA
TCCTTAACTGTTATAGAAACAGATGCGCGATGCCTCCCGCTCCTTAATGAGATTAAAGAATATTATCCTAATCTAAATAT
TATTAAACAAGATGCTCTTAAAATAAATTTAACCGATTTAAGCTATGATATAGTCAATTCAGTTGGATTTGCATACAAGA
AGCGAGAAGTGAAGCCTATAACTAATAGGAGGGCGAACGATATAGGCGAGTCTAAATCAATTGACTATAAAGTAACTATA
ATCTCTAATTTACCGTACCATATAGGTACTGAGTTAGTGATTAGATGGCTGAAAGAAGCACGGCTAATTACTAGTATGAC
GCTAATGTTACAAAAGGAAGTAGTAGAACGTATTTGTGCTATACCCTCAACTAAAGCATATGGCAGGTTATCGGTAATAT
GTCAGTTAATAGCTAAAGTGGAAAAGTGCTTTGACGTAGCGCCAACTGCTTTCTATCCACCTCCTAAAGTATATTCTGCG
ATAGTTAAACTAATACCTTTAGAAAATCCGCCGTCTATTGCTCTGATAAATAAAGTTGAGCAAATAACAAAGCTTGCTTT
TGCAGGACGACGGAAAATGATCAAATCATCACTGAAAAATCTTGTACCTAATATATATGAAGTATTAACTCAGTTGAAAA
TCAATGATAATTATCGCGCTGAAAATCTTGCCCCTCAAGATTATTTGAGAATTGCTGAAATATTATAG

Upstream 100 bases:

>100_bases
CTATGTCATTCCCGCGTAGGCGGGAATCCAGACAAATAACCTTGATTATTAATACAGAATTATATTTGATAAAAACAAAC
ATATAAAAACAAGTTTTTAT

Downstream 100 bases:

>100_bases
CTTGCATCATTCAGCTTTATCTAACTTTTTTGCTTCTGCTTCTTGCTGTAGTTTATATTCTGCTGCTTTTTCGTCTATAT
AGTTAAAAAAGAAATAACAG

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase [H]

Number of amino acids: Translated: 315; Mature: 315

Protein sequence:

>315_residues
MFYWIPAFTGMTYPMLPSIAKHAALHQVNPLKKHGQNFIFDSSLCDKIVRASNLAENSRVLEIGPGTGGLTRSILQKNPE
SLTVIETDARCLPLLNEIKEYYPNLNIIKQDALKINLTDLSYDIVNSVGFAYKKREVKPITNRRANDIGESKSIDYKVTI
ISNLPYHIGTELVIRWLKEARLITSMTLMLQKEVVERICAIPSTKAYGRLSVICQLIAKVEKCFDVAPTAFYPPPKVYSA
IVKLIPLENPPSIALINKVEQITKLAFAGRRKMIKSSLKNLVPNIYEVLTQLKINDNYRAENLAPQDYLRIAEIL

Sequences:

>Translated_315_residues
MFYWIPAFTGMTYPMLPSIAKHAALHQVNPLKKHGQNFIFDSSLCDKIVRASNLAENSRVLEIGPGTGGLTRSILQKNPE
SLTVIETDARCLPLLNEIKEYYPNLNIIKQDALKINLTDLSYDIVNSVGFAYKKREVKPITNRRANDIGESKSIDYKVTI
ISNLPYHIGTELVIRWLKEARLITSMTLMLQKEVVERICAIPSTKAYGRLSVICQLIAKVEKCFDVAPTAFYPPPKVYSA
IVKLIPLENPPSIALINKVEQITKLAFAGRRKMIKSSLKNLVPNIYEVLTQLKINDNYRAENLAPQDYLRIAEIL
>Mature_315_residues
MFYWIPAFTGMTYPMLPSIAKHAALHQVNPLKKHGQNFIFDSSLCDKIVRASNLAENSRVLEIGPGTGGLTRSILQKNPE
SLTVIETDARCLPLLNEIKEYYPNLNIIKQDALKINLTDLSYDIVNSVGFAYKKREVKPITNRRANDIGESKSIDYKVTI
ISNLPYHIGTELVIRWLKEARLITSMTLMLQKEVVERICAIPSTKAYGRLSVICQLIAKVEKCFDVAPTAFYPPPKVYSA
IVKLIPLENPPSIALINKVEQITKLAFAGRRKMIKSSLKNLVPNIYEVLTQLKINDNYRAENLAPQDYLRIAEIL

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits [H]

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily [H]

Homologues:

Organism=Homo sapiens, GI156415992, Length=279, Percent_Identity=32.9749103942652, Blast_Score=132, Evalue=3e-31,
Organism=Homo sapiens, GI7657198, Length=249, Percent_Identity=27.3092369477912, Blast_Score=91, Evalue=2e-18,
Organism=Escherichia coli, GI1786236, Length=285, Percent_Identity=36.140350877193, Blast_Score=153, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI25141369, Length=321, Percent_Identity=25.2336448598131, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI25146882, Length=265, Percent_Identity=30.188679245283, Blast_Score=100, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6324989, Length=278, Percent_Identity=26.6187050359712, Blast_Score=77, Evalue=3e-15,
Organism=Drosophila melanogaster, GI21357273, Length=321, Percent_Identity=32.7102803738318, Blast_Score=149, Evalue=3e-36,
Organism=Drosophila melanogaster, GI21358017, Length=263, Percent_Identity=27.7566539923954, Blast_Score=94, Evalue=9e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022436
- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530 [H]

Pfam domain/function: PF00398 RrnaAD [H]

EC number: =2.1.1.182 [H]

Molecular weight: Translated: 35573; Mature: 35573

Theoretical pI: Translated: 9.74; Mature: 9.74

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFYWIPAFTGMTYPMLPSIAKHAALHQVNPLKKHGQNFIFDSSLCDKIVRASNLAENSRV
CEEEECCHHCCCHHHHHHHHHHHHHHHCCHHHHCCCCCEECHHHHHHHHHHHCCCCCCEE
LEIGPGTGGLTRSILQKNPESLTVIETDARCLPLLNEIKEYYPNLNIIKQDALKINLTDL
EEECCCCCHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHCCCCCEEECCCEEEEEEHH
SYDIVNSVGFAYKKREVKPITNRRANDIGESKSIDYKVTIISNLPYHIGTELVIRWLKEA
HHHHHHHHHHHHHHCCCCCHHCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHH
RLITSMTLMLQKEVVERICAIPSTKAYGRLSVICQLIAKVEKCFDVAPTAFYPPPKVYSA
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
IVKLIPLENPPSIALINKVEQITKLAFAGRRKMIKSSLKNLVPNIYEVLTQLKINDNYRA
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
ENLAPQDYLRIAEIL
CCCCHHHHHHHHHHC
>Mature Secondary Structure
MFYWIPAFTGMTYPMLPSIAKHAALHQVNPLKKHGQNFIFDSSLCDKIVRASNLAENSRV
CEEEECCHHCCCHHHHHHHHHHHHHHHCCHHHHCCCCCEECHHHHHHHHHHHCCCCCCEE
LEIGPGTGGLTRSILQKNPESLTVIETDARCLPLLNEIKEYYPNLNIIKQDALKINLTDL
EEECCCCCHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHCCCCCEEECCCEEEEEEHH
SYDIVNSVGFAYKKREVKPITNRRANDIGESKSIDYKVTIISNLPYHIGTELVIRWLKEA
HHHHHHHHHHHHHHCCCCCHHCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHH
RLITSMTLMLQKEVVERICAIPSTKAYGRLSVICQLIAKVEKCFDVAPTAFYPPPKVYSA
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
IVKLIPLENPPSIALINKVEQITKLAFAGRRKMIKSSLKNLVPNIYEVLTQLKINDNYRA
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
ENLAPQDYLRIAEIL
CCCCHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA