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The map label for this gene is pbpA1 [H]

Identifier: 15892775

GI number: 15892775

Start: 803229

End: 805010

Strand: Reverse

Name: pbpA1 [H]

Synonym: RC0852

Alternate gene names: 15892775

Gene position: 805010-803229 (Counterclockwise)

Preceding gene: 15892776

Following gene: 15892774

Centisome position: 63.45

GC content: 34.51

Gene sequence:

>1782_bases
ATGCTAAATAAGAAAATACTACATGGTGAGTTAATTTCACGAAGAGCCTTTATAATCGGATTAGGTAAACTCGGATTTTT
ATCGTTGCTTGGAATAAGAATGTTTTATTTACAGCTTATTAAAAGCGAAGAATATAAGACTTTATCGGATAAGAATCGTA
TTAATTTTGTTGTACTTCCACCGACTAGAGGAAGAATTTACGATTTAGACGGCAATATTTTAGCTACTAATAAACCATGT
TATCAGCTAGTAATAGATAGAAGCATTAATAATAATTATAGGGATGAATTAGAAATAATTAGTAATATTCTAAATTTATC
TCCGGAAAAATATAATTATATTAAGCAAAAAATTAAAAAATCTAGTCGCCACACTCCTTTAATAATATTTGATCAGCTTG
ATTGGCAGCAAGTATCAATGATTGAAGAACAAAAGCATAAACTCGCTTCAATATTTATTGATATAGGTTATTTAAGGTTT
TATCCTTTTTCAAGCGTTACTTCTCATTTAATCGGTTATCTTGGTCAAATAAATGAGCACGAGAAGCAGGAATTAAATAT
ACATAGTTTAAGCGATTTTAATATCGGTAAATCCGGTATTGAAAAATATTACGATAACAAACTAAGAGGAGCATTTGGTT
ATAAAAAAGTTGAAGTAAACGCTTACGGTAAGCAGGTAAGAGAAATAGCAGGAACCCCTACTAAATCGGGCGAAGATATG
CATTTAAATATCGATGTGTCTCTTCAGCAAAATATCCAGCAATATTTAAATCCTAAAGGTTCTTCGGCAATAGTTATGGA
TACTAAAACCGGAAATGTACTAATTTGTGCTTCCACTCCGGGTTTTGAATCAAATCATTTCAGTAAATTATCGGAAAATT
ATTGGCAAAGTTTAACGAGTGATCCTTATAAGCCTTTAATTAATAAGGTAATACAAAATTCTTATCCTCCAGGCTCGGTT
TTTAAAATAATTACCGTACTTGCAGCCCTTGAAGTAGGCATTAATCCTAATAAAACCGTTTTTTGCGACGGTAGCTCTGC
TCTTGGTACTAATAGCTTTAGATGTTGGAATCATAGCGGACACGGAACGTTAGATATGATGTCTGCTTTAAAGCATTCTT
GTAATATTTATATGTATGAATTAGCAAGAATAGTAGGTCCGGATAAAATTCTTGAAGTTGCAAGAGAATTTGGCCTCGGT
TCAAAAACCGGTATTGATTTAGCTCCTGAAAGTAGCGGGTTTGTACCGTCAAAAGAGTGGAAAAAGAAAAAATTAAAGCT
TCCTTGGTCAATAGGAGATAGTTTTAATTTAGCAATCGGACAGGGGTTTCTAGGGGTAACGCCGATGCAGCTTGCAAGGT
TTATTACAGCTATTGCAAGTAACGGTAAACTGTATACGCCTAGAATATTAAAAAATGATCCGGAATTTTATAATGTCAAT
ATTAAGCCCGAAAATATCACAATAATCCAAGAAAGTTTGTATAATACCGTTAATGTTGCGGGAGGTACGGCGTATTATAA
TAGAATTTTAGGCAAAAATAGGCAGCTCGCTGGTAAAACCGGTACTTCGCAGGTGCAAGGCAAACTAAATGCTAAAGATG
ATTTAAGCCGTAAATCTATTGCTTGGGAGAGGCGTAATCATGCCTTGTTCTTAGGATTCGCTCCTTATCACGATCCTAGT
TATTCCGTTACCGTCTTTATTGATCATGGCGGAGGCGGTAGTAAAGCCGCCGCCCCTGTAGCTCGTAAAATTATGTCGGA
CGTGTTGGATAAGTATTTATAG

Upstream 100 bases:

>100_bases
GACGGATATTTAATAATTTTCTTTCAATTTTTAACAACTATATTTTCTTATAATATAATTCGTCTTGTACTTGACTCTCC
TATGAATTACTTTAAAAAGT

Downstream 100 bases:

>100_bases
CTTTTTTCAGTTTGTAGTTTGTGCTATTATGTGTCGTAAGTGTTATTAATTAAAGATAGGTCACAAGATGGCAAGACTGA
ATTTAACTATTCCGGACGAA

Product: penicillin-binding protein

Products: NA

Alternate protein names: PBP-2 [H]

Number of amino acids: Translated: 593; Mature: 593

Protein sequence:

>593_residues
MLNKKILHGELISRRAFIIGLGKLGFLSLLGIRMFYLQLIKSEEYKTLSDKNRINFVVLPPTRGRIYDLDGNILATNKPC
YQLVIDRSINNNYRDELEIISNILNLSPEKYNYIKQKIKKSSRHTPLIIFDQLDWQQVSMIEEQKHKLASIFIDIGYLRF
YPFSSVTSHLIGYLGQINEHEKQELNIHSLSDFNIGKSGIEKYYDNKLRGAFGYKKVEVNAYGKQVREIAGTPTKSGEDM
HLNIDVSLQQNIQQYLNPKGSSAIVMDTKTGNVLICASTPGFESNHFSKLSENYWQSLTSDPYKPLINKVIQNSYPPGSV
FKIITVLAALEVGINPNKTVFCDGSSALGTNSFRCWNHSGHGTLDMMSALKHSCNIYMYELARIVGPDKILEVAREFGLG
SKTGIDLAPESSGFVPSKEWKKKKLKLPWSIGDSFNLAIGQGFLGVTPMQLARFITAIASNGKLYTPRILKNDPEFYNVN
IKPENITIIQESLYNTVNVAGGTAYYNRILGKNRQLAGKTGTSQVQGKLNAKDDLSRKSIAWERRNHALFLGFAPYHDPS
YSVTVFIDHGGGGSKAAAPVARKIMSDVLDKYL

Sequences:

>Translated_593_residues
MLNKKILHGELISRRAFIIGLGKLGFLSLLGIRMFYLQLIKSEEYKTLSDKNRINFVVLPPTRGRIYDLDGNILATNKPC
YQLVIDRSINNNYRDELEIISNILNLSPEKYNYIKQKIKKSSRHTPLIIFDQLDWQQVSMIEEQKHKLASIFIDIGYLRF
YPFSSVTSHLIGYLGQINEHEKQELNIHSLSDFNIGKSGIEKYYDNKLRGAFGYKKVEVNAYGKQVREIAGTPTKSGEDM
HLNIDVSLQQNIQQYLNPKGSSAIVMDTKTGNVLICASTPGFESNHFSKLSENYWQSLTSDPYKPLINKVIQNSYPPGSV
FKIITVLAALEVGINPNKTVFCDGSSALGTNSFRCWNHSGHGTLDMMSALKHSCNIYMYELARIVGPDKILEVAREFGLG
SKTGIDLAPESSGFVPSKEWKKKKLKLPWSIGDSFNLAIGQGFLGVTPMQLARFITAIASNGKLYTPRILKNDPEFYNVN
IKPENITIIQESLYNTVNVAGGTAYYNRILGKNRQLAGKTGTSQVQGKLNAKDDLSRKSIAWERRNHALFLGFAPYHDPS
YSVTVFIDHGGGGSKAAAPVARKIMSDVLDKYL
>Mature_593_residues
MLNKKILHGELISRRAFIIGLGKLGFLSLLGIRMFYLQLIKSEEYKTLSDKNRINFVVLPPTRGRIYDLDGNILATNKPC
YQLVIDRSINNNYRDELEIISNILNLSPEKYNYIKQKIKKSSRHTPLIIFDQLDWQQVSMIEEQKHKLASIFIDIGYLRF
YPFSSVTSHLIGYLGQINEHEKQELNIHSLSDFNIGKSGIEKYYDNKLRGAFGYKKVEVNAYGKQVREIAGTPTKSGEDM
HLNIDVSLQQNIQQYLNPKGSSAIVMDTKTGNVLICASTPGFESNHFSKLSENYWQSLTSDPYKPLINKVIQNSYPPGSV
FKIITVLAALEVGINPNKTVFCDGSSALGTNSFRCWNHSGHGTLDMMSALKHSCNIYMYELARIVGPDKILEVAREFGLG
SKTGIDLAPESSGFVPSKEWKKKKLKLPWSIGDSFNLAIGQGFLGVTPMQLARFITAIASNGKLYTPRILKNDPEFYNVN
IKPENITIIQESLYNTVNVAGGTAYYNRILGKNRQLAGKTGTSQVQGKLNAKDDLSRKSIAWERRNHALFLGFAPYHDPS
YSVTVFIDHGGGGSKAAAPVARKIMSDVLDKYL

Specific function: Cell wall formation; PBP-2 is responsible for the determination of the rod shape of the cell. Its synthesize cross- linked peptidoglycan from the lipid intermediates [H]

COG id: COG0768

COG function: function code M; Cell division protein FtsI/penicillin-binding protein 2

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786854, Length=601, Percent_Identity=31.6139767054908, Blast_Score=328, Evalue=4e-91,
Organism=Escherichia coli, GI1786272, Length=605, Percent_Identity=26.9421487603306, Blast_Score=159, Evalue=4e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR005311
- InterPro:   IPR001460
- InterPro:   IPR017790 [H]

Pfam domain/function: PF03717 PBP_dimer; PF00905 Transpeptidase [H]

EC number: NA

Molecular weight: Translated: 66519; Mature: 66519

Theoretical pI: Translated: 9.78; Mature: 9.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNKKILHGELISRRAFIIGLGKLGFLSLLGIRMFYLQLIKSEEYKTLSDKNRINFVVLP
CCCCCCHHHHHHHCCEEEEEECHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEC
PTRGRIYDLDGNILATNKPCYQLVIDRSINNNYRDELEIISNILNLSPEKYNYIKQKIKK
CCCCEEEECCCCEEECCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHH
SSRHTPLIIFDQLDWQQVSMIEEQKHKLASIFIDIGYLRFYPFSSVTSHLIGYLGQINEH
HCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHEEEHHHEECCHHHHHHHHHHHHHCCCCC
EKQELNIHSLSDFNIGKSGIEKYYDNKLRGAFGYKKVEVNAYGKQVREIAGTPTKSGEDM
HHHHCCEEECCCCCCCHHHHHHHHCCHHCCCCCEEEEEECHHHHHHHHHHCCCCCCCCEE
HLNIDVSLQQNIQQYLNPKGSSAIVMDTKTGNVLICASTPGFESNHFSKLSENYWQSLTS
EEEEEEHHHHHHHHHHCCCCCEEEEEEECCCCEEEEECCCCCCCHHHHHHHHHHHHHHCC
DPYKPLINKVIQNSYPPGSVFKIITVLAALEVGINPNKTVFCDGSSALGTNSFRCWNHSG
CCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCEEEECCCC
HGTLDMMSALKHSCNIYMYELARIVGPDKILEVAREFGLGSKTGIDLAPESSGFVPSKEW
CCHHHHHHHHHHCCCCHHHHHHHHHCHHHHHHHHHHHCCCCCCCCEECCCCCCCCCCHHH
KKKKLKLPWSIGDSFNLAIGQGFLGVTPMQLARFITAIASNGKLYTPRILKNDPEFYNVN
HHHHCCCCEECCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCEECCHHHCCCCCEEEEE
IKPENITIIQESLYNTVNVAGGTAYYNRILGKNRQLAGKTGTSQVQGKLNAKDDLSRKSI
EECCCEEEEHHHHHCEEECCCCHHHHHHHHCCCCCCCCCCCCHHHCCCCCCCCCCCHHHH
AWERRNHALFLGFAPYHDPSYSVTVFIDHGGGGSKAAAPVARKIMSDVLDKYL
HHCCCCCEEEEEECCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLNKKILHGELISRRAFIIGLGKLGFLSLLGIRMFYLQLIKSEEYKTLSDKNRINFVVLP
CCCCCCHHHHHHHCCEEEEEECHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEC
PTRGRIYDLDGNILATNKPCYQLVIDRSINNNYRDELEIISNILNLSPEKYNYIKQKIKK
CCCCEEEECCCCEEECCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHH
SSRHTPLIIFDQLDWQQVSMIEEQKHKLASIFIDIGYLRFYPFSSVTSHLIGYLGQINEH
HCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHEEEHHHEECCHHHHHHHHHHHHHCCCCC
EKQELNIHSLSDFNIGKSGIEKYYDNKLRGAFGYKKVEVNAYGKQVREIAGTPTKSGEDM
HHHHCCEEECCCCCCCHHHHHHHHCCHHCCCCCEEEEEECHHHHHHHHHHCCCCCCCCEE
HLNIDVSLQQNIQQYLNPKGSSAIVMDTKTGNVLICASTPGFESNHFSKLSENYWQSLTS
EEEEEEHHHHHHHHHHCCCCCEEEEEEECCCCEEEEECCCCCCCHHHHHHHHHHHHHHCC
DPYKPLINKVIQNSYPPGSVFKIITVLAALEVGINPNKTVFCDGSSALGTNSFRCWNHSG
CCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCEEEECCCC
HGTLDMMSALKHSCNIYMYELARIVGPDKILEVAREFGLGSKTGIDLAPESSGFVPSKEW
CCHHHHHHHHHHCCCCHHHHHHHHHCHHHHHHHHHHHCCCCCCCCEECCCCCCCCCCHHH
KKKKLKLPWSIGDSFNLAIGQGFLGVTPMQLARFITAIASNGKLYTPRILKNDPEFYNVN
HHHHCCCCEECCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCEECCHHHCCCCCEEEEE
IKPENITIIQESLYNTVNVAGGTAYYNRILGKNRQLAGKTGTSQVQGKLNAKDDLSRKSI
EECCCEEEEHHHHHCEEECCCCHHHHHHHHCCCCCCCCCCCCHHHCCCCCCCCCCCHHHH
AWERRNHALFLGFAPYHDPSYSVTVFIDHGGGGSKAAAPVARKIMSDVLDKYL
HHCCCCCEEEEEECCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]