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The map label for this gene is ubiH [H]

Identifier: 15892771

GI number: 15892771

Start: 796142

End: 797437

Strand: Reverse

Name: ubiH [H]

Synonym: RC0848

Alternate gene names: 15892771

Gene position: 797437-796142 (Counterclockwise)

Preceding gene: 15892774

Following gene: 15892770

Centisome position: 62.85

GC content: 34.57

Gene sequence:

>1296_bases
ATGAATCGAATGAAAACAAAACAGATTGATAGACATCTTGCCAAATTCGCTTATAGCGAGGAATTTGTAGGAGACACGAA
ACGCAGCACCGCAGCGTACACAAGCGTACGTGAGGATGCGAGTATCGGATCGACGCACAAATTACCTCTAGAAGTAGAAG
AGTTTGGGAAGATGTCTAATACGGTAATTTTAGGATGCGGTCTTAGCGGCATGTTAACGGCGCTTTCTTTTGCACAAAAG
GGTATAAAAACTACTATATTCGAGAGTAAATCGGTAAAAAGCCCAGAGTTTTTTAAAGATATAAGAACTACTGCTTTAAC
TCCGCATTCTAAAAATTTCTTATTCTCTATTGATATATGGGAAGAGCTTGAGAAGTTTGTAGCAGAAATGCAAGACATAT
ATGTAGTAGATAATCAGGCTTCAGAGATATTAGACTTACGAAATGACGCTGACGCTGTACTTGGATATGTGGTTAAGAAT
AGCGACTTTAAAAAAATGCTATTGTCAAAAATAACTAATAATCCGTTGATAACATTAATTGATAATAATCAATATCAAGA
GGTTATAAGCCATAATGACTACTCGATTATAAAATTTGATGATAACCAAATTAAGTGTAATTTATTAATTATATGTGACG
GAGCAAATTCAAAAGTGAGGTCTCATTACTTTGCTAATGAAATTGAGAAACCTTATCAAACTGCTCTTACATTTAATATT
AAGCATGAAAAGCCGCATGAAAATTGTGCTATGGAGCATTTCCTGCCGTTTGGTCCTTTTGCTTTGCTGCCTTTAAAAGA
TCAATATGCTTCATCCGTAATATGGTCAACTTCTTCTGATCAGGCTGCTTTAATTGTTAATTTGCCTGTAGAAGAAGTTA
GGTTTTTAACTCAAAGAAATGCCGGTAATTCCCTAGGTAAAATTACTATTGATAGTGAGATTAGTAGCTTTCCACTAAAA
GCCCGTATAGCAAATAGATATTTTCATAACCGTATAGTGCTTATTGCCGACACTGCCCACACAGTACATCCGCTAGCCGG
ACAAGGGCTTAATCAGGGCATAAAGGATATAGAGATTTTGAGTATGATAGTTAGTAATAATGGCCCATTACAAGAATATC
AAAAGTTAAGGCAAGAGGATAATTTTATTATGTATAAGCTGACTGATGAGTTAAATAATATTTTTTCAAATCATTCCAAA
AATTTAAGATGTTTAAGACAAATAGGATTTAAGGTGATCAATAATTTTAAACCTGTAAAAAACTTGATTACTAGCTATGC
GATGGGGAAGAGGTGA

Upstream 100 bases:

>100_bases
TTAGGTTTATACTCAGGCAATAGTTCATATTTGGATATAGCAATTATTTATTTTCTTCTAAGCTTTATAGCAACAAACGG
ATATTTAAAATATTTCATAA

Downstream 100 bases:

>100_bases
GTGTTCTTTTGTATCATTTCTAGCTAGTTCTGATGTCATTCCCGCGAAAGTGGGAATCCAGTAATCAAGAATCGATAAAA
GCATTGTTTGTTTATACTTT

Product: 2-octaprenyl-6-methoxyphenyl hydroxylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MNRMKTKQIDRHLAKFAYSEEFVGDTKRSTAAYTSVREDASIGSTHKLPLEVEEFGKMSNTVILGCGLSGMLTALSFAQK
GIKTTIFESKSVKSPEFFKDIRTTALTPHSKNFLFSIDIWEELEKFVAEMQDIYVVDNQASEILDLRNDADAVLGYVVKN
SDFKKMLLSKITNNPLITLIDNNQYQEVISHNDYSIIKFDDNQIKCNLLIICDGANSKVRSHYFANEIEKPYQTALTFNI
KHEKPHENCAMEHFLPFGPFALLPLKDQYASSVIWSTSSDQAALIVNLPVEEVRFLTQRNAGNSLGKITIDSEISSFPLK
ARIANRYFHNRIVLIADTAHTVHPLAGQGLNQGIKDIEILSMIVSNNGPLQEYQKLRQEDNFIMYKLTDELNNIFSNHSK
NLRCLRQIGFKVINNFKPVKNLITSYAMGKR

Sequences:

>Translated_431_residues
MNRMKTKQIDRHLAKFAYSEEFVGDTKRSTAAYTSVREDASIGSTHKLPLEVEEFGKMSNTVILGCGLSGMLTALSFAQK
GIKTTIFESKSVKSPEFFKDIRTTALTPHSKNFLFSIDIWEELEKFVAEMQDIYVVDNQASEILDLRNDADAVLGYVVKN
SDFKKMLLSKITNNPLITLIDNNQYQEVISHNDYSIIKFDDNQIKCNLLIICDGANSKVRSHYFANEIEKPYQTALTFNI
KHEKPHENCAMEHFLPFGPFALLPLKDQYASSVIWSTSSDQAALIVNLPVEEVRFLTQRNAGNSLGKITIDSEISSFPLK
ARIANRYFHNRIVLIADTAHTVHPLAGQGLNQGIKDIEILSMIVSNNGPLQEYQKLRQEDNFIMYKLTDELNNIFSNHSK
NLRCLRQIGFKVINNFKPVKNLITSYAMGKR
>Mature_431_residues
MNRMKTKQIDRHLAKFAYSEEFVGDTKRSTAAYTSVREDASIGSTHKLPLEVEEFGKMSNTVILGCGLSGMLTALSFAQK
GIKTTIFESKSVKSPEFFKDIRTTALTPHSKNFLFSIDIWEELEKFVAEMQDIYVVDNQASEILDLRNDADAVLGYVVKN
SDFKKMLLSKITNNPLITLIDNNQYQEVISHNDYSIIKFDDNQIKCNLLIICDGANSKVRSHYFANEIEKPYQTALTFNI
KHEKPHENCAMEHFLPFGPFALLPLKDQYASSVIWSTSSDQAALIVNLPVEEVRFLTQRNAGNSLGKITIDSEISSFPLK
ARIANRYFHNRIVLIADTAHTVHPLAGQGLNQGIKDIEILSMIVSNNGPLQEYQKLRQEDNFIMYKLTDELNNIFSNHSK
NLRCLRQIGFKVINNFKPVKNLITSYAMGKR

Specific function: Oxygenase that introduces the hydroxyl group at carbon five of 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol resulting in the formation of 2-octaprenyl-3-methyl-5-hydroxy-6-methoxy-1,4- benzoquinol [H]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ubiH/COQ6 family [H]

Homologues:

Organism=Homo sapiens, GI32967305, Length=388, Percent_Identity=27.0618556701031, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI32967307, Length=276, Percent_Identity=30.7971014492754, Blast_Score=112, Evalue=6e-25,
Organism=Escherichia coli, GI1786883, Length=397, Percent_Identity=27.7078085642317, Blast_Score=148, Evalue=8e-37,
Organism=Escherichia coli, GI1789273, Length=393, Percent_Identity=26.7175572519084, Blast_Score=141, Evalue=9e-35,
Organism=Escherichia coli, GI1789274, Length=390, Percent_Identity=25.6410256410256, Blast_Score=139, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI25146631, Length=440, Percent_Identity=25.4545454545455, Blast_Score=114, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6321694, Length=453, Percent_Identity=23.841059602649, Blast_Score=100, Evalue=6e-22,
Organism=Drosophila melanogaster, GI19920760, Length=383, Percent_Identity=25.8485639686684, Blast_Score=114, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042
- InterPro:   IPR018168
- InterPro:   IPR010971 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: 1.14.13.-

Molecular weight: Translated: 48922; Mature: 48922

Theoretical pI: Translated: 7.95; Mature: 7.95

Prosite motif: PS01304 UBIH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRMKTKQIDRHLAKFAYSEEFVGDTKRSTAAYTSVREDASIGSTHKLPLEVEEFGKMSN
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCC
TVILGCGLSGMLTALSFAQKGIKTTIFESKSVKSPEFFKDIRTTALTPHSKNFLFSIDIW
EEEEECCHHHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHCCCCCCCCEEEEEHHH
EELEKFVAEMQDIYVVDNQASEILDLRNDADAVLGYVVKNSDFKKMLLSKITNNPLITLI
HHHHHHHHHHHHEEEECCCHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHCCCCEEEEE
DNNQYQEVISHNDYSIIKFDDNQIKCNLLIICDGANSKVRSHYFANEIEKPYQTALTFNI
ECCHHHHHHCCCCEEEEEEECCEEEEEEEEEECCCCCHHHHHHHHHHHCCCCEEEEEEEE
KHEKPHENCAMEHFLPFGPFALLPLKDQYASSVIWSTSSDQAALIVNLPVEEVRFLTQRN
CCCCCCHHHHHHHCCCCCCEEEEECCHHHHCCEEEECCCCCEEEEEECCHHHHHHHHHCC
AGNSLGKITIDSEISSFPLKARIANRYFHNRIVLIADTAHTVHPLAGQGLNQGIKDIEIL
CCCCCCEEEECCCCCCCCHHHHHHHHHHHCEEEEEECCCCCCCCCCCCCHHCCHHHHHHH
SMIVSNNGPLQEYQKLRQEDNFIMYKLTDELNNIFSNHSKNLRCLRQIGFKVINNFKPVK
HHHHCCCCCHHHHHHHHCCCCEEEEEEHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHH
NLITSYAMGKR
HHHHHHHCCCC
>Mature Secondary Structure
MNRMKTKQIDRHLAKFAYSEEFVGDTKRSTAAYTSVREDASIGSTHKLPLEVEEFGKMSN
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCC
TVILGCGLSGMLTALSFAQKGIKTTIFESKSVKSPEFFKDIRTTALTPHSKNFLFSIDIW
EEEEECCHHHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHCCCCCCCCEEEEEHHH
EELEKFVAEMQDIYVVDNQASEILDLRNDADAVLGYVVKNSDFKKMLLSKITNNPLITLI
HHHHHHHHHHHHEEEECCCHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHCCCCEEEEE
DNNQYQEVISHNDYSIIKFDDNQIKCNLLIICDGANSKVRSHYFANEIEKPYQTALTFNI
ECCHHHHHHCCCCEEEEEEECCEEEEEEEEEECCCCCHHHHHHHHHHHCCCCEEEEEEEE
KHEKPHENCAMEHFLPFGPFALLPLKDQYASSVIWSTSSDQAALIVNLPVEEVRFLTQRN
CCCCCCHHHHHHHCCCCCCEEEEECCHHHHCCEEEECCCCCEEEEEECCHHHHHHHHHCC
AGNSLGKITIDSEISSFPLKARIANRYFHNRIVLIADTAHTVHPLAGQGLNQGIKDIEIL
CCCCCCEEEECCCCCCCCHHHHHHHHHHHCEEEEEECCCCCCCCCCCCCHHCCHHHHHHH
SMIVSNNGPLQEYQKLRQEDNFIMYKLTDELNNIFSNHSKNLRCLRQIGFKVINNFKPVK
HHHHCCCCCHHHHHHHHCCCCEEEEEEHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHH
NLITSYAMGKR
HHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydroxylation [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 10802164 [H]