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The map label for this gene is spl1 [H]

Identifier: 15892653

GI number: 15892653

Start: 702078

End: 703310

Strand: Reverse

Name: spl1 [H]

Synonym: RC0730

Alternate gene names: 15892653

Gene position: 703310-702078 (Counterclockwise)

Preceding gene: 15892654

Following gene: 15892652

Centisome position: 55.43

GC content: 37.88

Gene sequence:

>1233_bases
ATGAACCCACAATTAAACAATTTAACCTTACCGATATATATGGATTATCAGGCGACGACTCCAATAGATCCAAGAGTAAT
GGAGGCAATGTTGCCTTATTTTACTACCAAGTTCGGCAATCCTCATTCACGCAGCCATTCTTTCGGTTGGGAGGCAGAAA
ACGCCGTTGAAGAGGCAAGGAGTATGGTAGCAAAGTTAATAGGAGCGGATACTAAAGAAATTATTTTCACTTCCGGTGCA
ACTGAGTCTAACAATCTTGCAATAAAGGGAATAGCAAAATTTTACAGCAATAAAAAGAATCATATTATTACCGTAGTCAG
TGAACATAAATGCGTACTTGATGCTTGCAGGCATTTAGAGCAAGAAGGTATAAAAATCACATACTTACCGATTAAGCCAA
ATGGAATAATCGATTTAGAAACTCTAAAAAATGCCATTACCGATCAGACTATGTTAGTTTCAGTTATGGTGGTTAATAAT
GAAATAGGTGTTGTTCAGCCTTTAAAGGAAATCGGGAAAATTTGCCGTGAAAAGGGCGTTTTTTTTCATTCCGATATTGC
TCAAGGTTTTGGTAAAATTCCAATTGATGTTAACGCATTTAATATTGATCTTGCCAGTATCTCAGGGCATAAAATTTACG
GTCCGAAAGGAATAGGTGCATTATATGTAAGGAAAAAACCTCGTGTGCGTGTTACACCGCTCATAAACGGTGGCGGGCAG
GAGAGAGGTATGCGTTCAGGTACGCTACCGACTCCTTTAATCGTAGGGCTTGGCATGGCTGCTGAAATAGCGTATAGTGA
GATGGAAAAAGATACTAAGCACGTAAATTACTTATTCGACAGATTTTTAAATAATATACATAAGCGAATTTCAGAAGTTT
ATTTAAACGGCGATAAAAATCAAAGATATAAAGGTAATTTAAATTTAAGCTTTGCCGGAGTTGAAGGAGAATCAATGATC
CTTGCCATTAAAGATTTAGCGGTTTCCTCCGGTTCTGCTTGTACTTCTGCCTCTTTAGAACCGTCATATGTTTTACGTTC
TATGGGAATCGGTGAAGAGCTTGCCCATACTGCAATTAGGTTCGGCATAGGTAGATTTACTACCGAGCAGGAAGTTGACT
ACGCAGTAAATTTAATATGCTCAAAAATCGATAAACTAAGGGAATTAAGCCCTCTTTGGGAAATGATGCAAGAGGGAATT
GATTTGAAAAAGATTAAATGGGCTGTACATTGA

Upstream 100 bases:

>100_bases
GCTACGTTGTTGCACGGCTCCGTTTATGTCATTCCCGCTTCCGCAGGAATGACATCGATGACTGTTTAAACTATACCAAA
ACATGAATTAATTTAACCAT

Downstream 100 bases:

>100_bases
GAATATCGGCATTGCGAGCAGCCGTAGGCTGCGTGGCAATCTTGTCAAATATCCTGAGATTGCTTCGTCAAAACTTGCAG
TGTTTCCTCGCAATGACGGC

Product: cysteine desulfurase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 410; Mature: 410

Protein sequence:

>410_residues
MNPQLNNLTLPIYMDYQATTPIDPRVMEAMLPYFTTKFGNPHSRSHSFGWEAENAVEEARSMVAKLIGADTKEIIFTSGA
TESNNLAIKGIAKFYSNKKNHIITVVSEHKCVLDACRHLEQEGIKITYLPIKPNGIIDLETLKNAITDQTMLVSVMVVNN
EIGVVQPLKEIGKICREKGVFFHSDIAQGFGKIPIDVNAFNIDLASISGHKIYGPKGIGALYVRKKPRVRVTPLINGGGQ
ERGMRSGTLPTPLIVGLGMAAEIAYSEMEKDTKHVNYLFDRFLNNIHKRISEVYLNGDKNQRYKGNLNLSFAGVEGESMI
LAIKDLAVSSGSACTSASLEPSYVLRSMGIGEELAHTAIRFGIGRFTTEQEVDYAVNLICSKIDKLRELSPLWEMMQEGI
DLKKIKWAVH

Sequences:

>Translated_410_residues
MNPQLNNLTLPIYMDYQATTPIDPRVMEAMLPYFTTKFGNPHSRSHSFGWEAENAVEEARSMVAKLIGADTKEIIFTSGA
TESNNLAIKGIAKFYSNKKNHIITVVSEHKCVLDACRHLEQEGIKITYLPIKPNGIIDLETLKNAITDQTMLVSVMVVNN
EIGVVQPLKEIGKICREKGVFFHSDIAQGFGKIPIDVNAFNIDLASISGHKIYGPKGIGALYVRKKPRVRVTPLINGGGQ
ERGMRSGTLPTPLIVGLGMAAEIAYSEMEKDTKHVNYLFDRFLNNIHKRISEVYLNGDKNQRYKGNLNLSFAGVEGESMI
LAIKDLAVSSGSACTSASLEPSYVLRSMGIGEELAHTAIRFGIGRFTTEQEVDYAVNLICSKIDKLRELSPLWEMMQEGI
DLKKIKWAVH
>Mature_410_residues
MNPQLNNLTLPIYMDYQATTPIDPRVMEAMLPYFTTKFGNPHSRSHSFGWEAENAVEEARSMVAKLIGADTKEIIFTSGA
TESNNLAIKGIAKFYSNKKNHIITVVSEHKCVLDACRHLEQEGIKITYLPIKPNGIIDLETLKNAITDQTMLVSVMVVNN
EIGVVQPLKEIGKICREKGVFFHSDIAQGFGKIPIDVNAFNIDLASISGHKIYGPKGIGALYVRKKPRVRVTPLINGGGQ
ERGMRSGTLPTPLIVGLGMAAEIAYSEMEKDTKHVNYLFDRFLNNIHKRISEVYLNGDKNQRYKGNLNLSFAGVEGESMI
LAIKDLAVSSGSACTSASLEPSYVLRSMGIGEELAHTAIRFGIGRFTTEQEVDYAVNLICSKIDKLRELSPLWEMMQEGI
DLKKIKWAVH

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=408, Percent_Identity=63.4803921568627, Blast_Score=575, Evalue=1e-164,
Organism=Homo sapiens, GI156713448, Length=416, Percent_Identity=35.0961538461538, Blast_Score=221, Evalue=7e-58,
Organism=Escherichia coli, GI48994898, Length=405, Percent_Identity=60, Blast_Score=510, Evalue=1e-146,
Organism=Escherichia coli, GI1789175, Length=399, Percent_Identity=26.3157894736842, Blast_Score=112, Evalue=6e-26,
Organism=Escherichia coli, GI1787970, Length=405, Percent_Identity=25.4320987654321, Blast_Score=107, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI25143064, Length=401, Percent_Identity=61.8453865336658, Blast_Score=541, Evalue=1e-154,
Organism=Caenorhabditis elegans, GI17533177, Length=330, Percent_Identity=34.2424242424242, Blast_Score=171, Evalue=5e-43,
Organism=Saccharomyces cerevisiae, GI6319831, Length=402, Percent_Identity=59.2039800995025, Blast_Score=519, Evalue=1e-148,
Organism=Drosophila melanogaster, GI20129463, Length=400, Percent_Identity=65, Blast_Score=580, Evalue=1e-166,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR010240
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 45475; Mature: 45475

Theoretical pI: Translated: 8.21; Mature: 8.21

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPQLNNLTLPIYMDYQATTPIDPRVMEAMLPYFTTKFGNPHSRSHSFGWEAENAVEEAR
CCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHH
SMVAKLIGADTKEIIFTSGATESNNLAIKGIAKFYSNKKNHIITVVSEHKCVLDACRHLE
HHHHHHHCCCCHHEEEECCCCCCCCEEEEEHHHHHCCCCCEEEEEEECCHHHHHHHHHHH
QEGIKITYLPIKPNGIIDLETLKNAITDQTMLVSVMVVNNEIGVVQPLKEIGKICREKGV
HCCCEEEEEEECCCCCEEHHHHHHHHCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHCCC
FFHSDIAQGFGKIPIDVNAFNIDLASISGHKIYGPKGIGALYVRKKPRVRVTPLINGGGQ
EEECHHHHCCCCCCEEEEEEEEEEEECCCCEEECCCCCCEEEEECCCCEEEEEEECCCCC
ERGMRSGTLPTPLIVGLGMAAEIAYSEMEKDTKHVNYLFDRFLNNIHKRISEVYLNGDKN
CCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCC
QRYKGNLNLSFAGVEGESMILAIKDLAVSSGSACTSASLEPSYVLRSMGIGEELAHTAIR
CCEECCEEEEEECCCCCEEEEEEEEHHCCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHH
FGIGRFTTEQEVDYAVNLICSKIDKLRELSPLWEMMQEGIDLKKIKWAVH
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHEEEECC
>Mature Secondary Structure
MNPQLNNLTLPIYMDYQATTPIDPRVMEAMLPYFTTKFGNPHSRSHSFGWEAENAVEEAR
CCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHH
SMVAKLIGADTKEIIFTSGATESNNLAIKGIAKFYSNKKNHIITVVSEHKCVLDACRHLE
HHHHHHHCCCCHHEEEECCCCCCCCEEEEEHHHHHCCCCCEEEEEEECCHHHHHHHHHHH
QEGIKITYLPIKPNGIIDLETLKNAITDQTMLVSVMVVNNEIGVVQPLKEIGKICREKGV
HCCCEEEEEEECCCCCEEHHHHHHHHCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHCCC
FFHSDIAQGFGKIPIDVNAFNIDLASISGHKIYGPKGIGALYVRKKPRVRVTPLINGGGQ
EEECHHHHCCCCCCEEEEEEEEEEEECCCCEEECCCCCCEEEEECCCCEEEEEEECCCCC
ERGMRSGTLPTPLIVGLGMAAEIAYSEMEKDTKHVNYLFDRFLNNIHKRISEVYLNGDKN
CCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCC
QRYKGNLNLSFAGVEGESMILAIKDLAVSSGSACTSASLEPSYVLRSMGIGEELAHTAIR
CCEECCEEEEEECCCCCEEEEEEEEHHCCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHH
FGIGRFTTEQEVDYAVNLICSKIDKLRELSPLWEMMQEGIDLKKIKWAVH
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA