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The map label for this gene is pnp

Identifier: 15892586

GI number: 15892586

Start: 642584

End: 644833

Strand: Reverse

Name: pnp

Synonym: RC0663

Alternate gene names: 15892586

Gene position: 644833-642584 (Counterclockwise)

Preceding gene: 15892587

Following gene: 15892585

Centisome position: 50.82

GC content: 34.84

Gene sequence:

>2250_bases
ATGTTTAACGAAATAACTAAGAGTGTTACATGGAACGGCCAAGTTCTTGAGCTTAGTACAGGTAAAATAGCAAGGCAAGC
TGACGGGGCAGTTACAGTAAAAATGGGTAATTCCGTTTTATTATGTACGGCAGTAGTAGCTAACAAAGCAAAAGAGGGCA
TAGGTTTTCTTCCTTTAACAATTAATTATAGAGAAATGGCATATGCTGCCGGTAAGATACCAGGGGGATTTTTTAAACAT
GAGGGAAAAGCATCAGATAGAGAAGTTTTAGTATCTCGTTTAATAGATAGACCGATTAGACCATTATTTCATCCGGCTTT
TGTGAATGAAACGCATGTAACTTGCAGTGTTCTTTCATATGATCCTGAAACTCCGGTAGATATACTTGCAATTATCGGTG
CATCGGCTGCACTTAGCCTATCCCCTGCTCCTTATCTAGAAATAGTTGCGGCAAGTAAAGTAGGTTTAATCAATGGTGAA
TTTGTTTTAAATCCGACACTTGCATTATTAAAAACAAGTCAGCTTGATTTAGTAGTTGCAGGAACATCAGATTCAGTAAT
GATGGTTGAATCGGAAGCTCATTTACTTTCTGAAGAACAAATGTTAGAAGCCGTAAAATTTGGATTTGAAAGCTTTCAGC
CGGTAATAAAGATAATTAAAGAACTAGCAGAAGAAGCTAAAAAACCAAAACTTGAAATGCAAGCTTTATATCCTGCTTCA
TTAAAGAAAGAAATTGAGAAGTTATTTGTAAAAGAAATTGAACAAGCTTTTGCAATTAAATCTAAACAAGAACGTAGCAC
TAATTTAGATTTAATACCTGAAAAAGTTCTTACACATTTTGTAAGCGACATAGAAAATAAAAAATATAGTAATTATCAAA
TCGAATCAGCTTTAAAAGCTATTGAATCAGATATATTACGTAACGAGATTTTAGAAAAAAATAGACGTATCGATGGAAGA
AGTACCACGGATATAAGACAAATTGCTTGTGAAATAGGTTTACTACCTTCCGCACATGGTTCAGCTTTATTTACTAGAGG
TGAAACGCAAAGCTTAGTTAGTACTACATTCGGTACTAGTTTAGATGAGCAGATAGTTGACAGTTTAGAGGGTGAGTATA
AAGAGCGTTTTATGCTCAATTATATCTTTCCGCCCTACTCTGTAAATGAAGCAATGCCAATGAAAGCACCTAGTCGTCGT
GAAGTCGGACACGGTAAACTCGCATGGCGTGCTATTAATCCAATATTACCTAATAAAGTACAATTTCCTTATTCTATTAG
AGTAGTTGCCGAAACTACGGAATCTAACGGTTCTTCTTCAATGGCAACTGTTTGCGGTAGTTCTCTTGCTTTAATGTATG
CTGGCGTACCGATAAAAGCACCGGTTGCGGGTATTGCTATGGGACTTGTTAAGGAAGGTAAAAATTTTGCCGTATTATCG
GATATTCTCGGCGATGAAGATTATTTCGGTGATATGGACTTTAAGGTTGCAGGAACTAGCGAAGGGATTACTGCATTACA
AATGGATATCAAAATATCCGGAGTAGATTTTAAAATAATGAAAGTAGCTTTAGAGCAAGCACGACTCGGTCGTTTGCATA
TACTGGAGCAAATGAATAAAGTTATCAGTAAACCAAATAACGAACTAAGTAAAAATGCTCCTTCTACTACTACTATAAAA
ATAGATAAGGATAAAATTAGAGATATTATAGGACCGGGCGGTAAAATAATAAAGGAAATTTGTGAAACTAGCGGTGCTAA
AATAGATATAAGCGATGACGGTACGGTGTCTGTTTATGCTTCAGATAGAGATAAGCTAAAAGTTGCCTTAGATAAAATTA
AAGCTATTGTCGTTGAACCTGAAATCGGTGAAATATTTAACGGTACGGTAGTGAAAGTCTTAGATTCCGGTGCTTTTATT
AATTATGTAGGTAATAAAGACGGCTTTGTTCATATTAGTGAAGTATCAGGAGAAAGAATAGAAACGGTTAGCAGTGTTTT
AAAACAAGGCGATATAGTAAAAGTTAAGCTAATAGGCTTTGATAATAAAGGCAAAGCAAAATTAACTATTAAAAATGCCG
ATAAAGATAAATCTTCAAATAATACAAAGCCAAAAACTAATGTTAATAATACTAATAAGGACAATTCAGAACCTGAGCAG
CGACGTGATTCTAGTAAAAAACGAGCTTGGAATGAAGATAATAATGCTGAAACTGCTGAAGTAATTACAGAACGTAAGTA
TTTTAATTGA

Upstream 100 bases:

>100_bases
TCAAATATCCTGAGATTGCTTCGTCGAATTACTTCGTAATTTTCCTCGCAATGACGTGGTAGAATACAATAAAATATAAA
TTATGAAATGAGATAAATAG

Downstream 100 bases:

>100_bases
TATGAAGCTTAATCGTCATTGCGAGGAAGTTACGAAGTAATTGACGAAGCAATCTCAGGAGTTTGTTATTATTTCATGAG
ATTGCCGTGTAGCCTACGGC

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase

Number of amino acids: Translated: 749; Mature: 749

Protein sequence:

>749_residues
MFNEITKSVTWNGQVLELSTGKIARQADGAVTVKMGNSVLLCTAVVANKAKEGIGFLPLTINYREMAYAAGKIPGGFFKH
EGKASDREVLVSRLIDRPIRPLFHPAFVNETHVTCSVLSYDPETPVDILAIIGASAALSLSPAPYLEIVAASKVGLINGE
FVLNPTLALLKTSQLDLVVAGTSDSVMMVESEAHLLSEEQMLEAVKFGFESFQPVIKIIKELAEEAKKPKLEMQALYPAS
LKKEIEKLFVKEIEQAFAIKSKQERSTNLDLIPEKVLTHFVSDIENKKYSNYQIESALKAIESDILRNEILEKNRRIDGR
STTDIRQIACEIGLLPSAHGSALFTRGETQSLVSTTFGTSLDEQIVDSLEGEYKERFMLNYIFPPYSVNEAMPMKAPSRR
EVGHGKLAWRAINPILPNKVQFPYSIRVVAETTESNGSSSMATVCGSSLALMYAGVPIKAPVAGIAMGLVKEGKNFAVLS
DILGDEDYFGDMDFKVAGTSEGITALQMDIKISGVDFKIMKVALEQARLGRLHILEQMNKVISKPNNELSKNAPSTTTIK
IDKDKIRDIIGPGGKIIKEICETSGAKIDISDDGTVSVYASDRDKLKVALDKIKAIVVEPEIGEIFNGTVVKVLDSGAFI
NYVGNKDGFVHISEVSGERIETVSSVLKQGDIVKVKLIGFDNKGKAKLTIKNADKDKSSNNTKPKTNVNNTNKDNSEPEQ
RRDSSKKRAWNEDNNAETAEVITERKYFN

Sequences:

>Translated_749_residues
MFNEITKSVTWNGQVLELSTGKIARQADGAVTVKMGNSVLLCTAVVANKAKEGIGFLPLTINYREMAYAAGKIPGGFFKH
EGKASDREVLVSRLIDRPIRPLFHPAFVNETHVTCSVLSYDPETPVDILAIIGASAALSLSPAPYLEIVAASKVGLINGE
FVLNPTLALLKTSQLDLVVAGTSDSVMMVESEAHLLSEEQMLEAVKFGFESFQPVIKIIKELAEEAKKPKLEMQALYPAS
LKKEIEKLFVKEIEQAFAIKSKQERSTNLDLIPEKVLTHFVSDIENKKYSNYQIESALKAIESDILRNEILEKNRRIDGR
STTDIRQIACEIGLLPSAHGSALFTRGETQSLVSTTFGTSLDEQIVDSLEGEYKERFMLNYIFPPYSVNEAMPMKAPSRR
EVGHGKLAWRAINPILPNKVQFPYSIRVVAETTESNGSSSMATVCGSSLALMYAGVPIKAPVAGIAMGLVKEGKNFAVLS
DILGDEDYFGDMDFKVAGTSEGITALQMDIKISGVDFKIMKVALEQARLGRLHILEQMNKVISKPNNELSKNAPSTTTIK
IDKDKIRDIIGPGGKIIKEICETSGAKIDISDDGTVSVYASDRDKLKVALDKIKAIVVEPEIGEIFNGTVVKVLDSGAFI
NYVGNKDGFVHISEVSGERIETVSSVLKQGDIVKVKLIGFDNKGKAKLTIKNADKDKSSNNTKPKTNVNNTNKDNSEPEQ
RRDSSKKRAWNEDNNAETAEVITERKYFN
>Mature_749_residues
MFNEITKSVTWNGQVLELSTGKIARQADGAVTVKMGNSVLLCTAVVANKAKEGIGFLPLTINYREMAYAAGKIPGGFFKH
EGKASDREVLVSRLIDRPIRPLFHPAFVNETHVTCSVLSYDPETPVDILAIIGASAALSLSPAPYLEIVAASKVGLINGE
FVLNPTLALLKTSQLDLVVAGTSDSVMMVESEAHLLSEEQMLEAVKFGFESFQPVIKIIKELAEEAKKPKLEMQALYPAS
LKKEIEKLFVKEIEQAFAIKSKQERSTNLDLIPEKVLTHFVSDIENKKYSNYQIESALKAIESDILRNEILEKNRRIDGR
STTDIRQIACEIGLLPSAHGSALFTRGETQSLVSTTFGTSLDEQIVDSLEGEYKERFMLNYIFPPYSVNEAMPMKAPSRR
EVGHGKLAWRAINPILPNKVQFPYSIRVVAETTESNGSSSMATVCGSSLALMYAGVPIKAPVAGIAMGLVKEGKNFAVLS
DILGDEDYFGDMDFKVAGTSEGITALQMDIKISGVDFKIMKVALEQARLGRLHILEQMNKVISKPNNELSKNAPSTTTIK
IDKDKIRDIIGPGGKIIKEICETSGAKIDISDDGTVSVYASDRDKLKVALDKIKAIVVEPEIGEIFNGTVVKVLDSGAFI
NYVGNKDGFVHISEVSGERIETVSSVLKQGDIVKVKLIGFDNKGKAKLTIKNADKDKSSNNTKPKTNVNNTNKDNSEPEQ
RRDSSKKRAWNEDNNAETAEVITERKYFN

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain

Homologues:

Organism=Homo sapiens, GI188528628, Length=712, Percent_Identity=37.2191011235955, Blast_Score=440, Evalue=1e-123,
Organism=Escherichia coli, GI145693187, Length=695, Percent_Identity=50.0719424460432, Blast_Score=687, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115534063, Length=726, Percent_Identity=31.5426997245179, Blast_Score=332, Evalue=7e-91,
Organism=Caenorhabditis elegans, GI17535281, Length=92, Percent_Identity=39.1304347826087, Blast_Score=65, Evalue=1e-10,
Organism=Drosophila melanogaster, GI281362905, Length=699, Percent_Identity=35.0500715307582, Blast_Score=415, Evalue=1e-116,
Organism=Drosophila melanogaster, GI24651641, Length=699, Percent_Identity=35.0500715307582, Blast_Score=415, Evalue=1e-116,
Organism=Drosophila melanogaster, GI24651643, Length=699, Percent_Identity=35.0500715307582, Blast_Score=415, Evalue=1e-116,
Organism=Drosophila melanogaster, GI161079377, Length=688, Percent_Identity=34.0116279069767, Blast_Score=377, Evalue=1e-104,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): PNP_RICCN (Q92HV7)

Other databases:

- EMBL:   AE006914
- PIR:   G97782
- RefSeq:   NP_360300.1
- HSSP:   P05055
- ProteinModelPortal:   Q92HV7
- SMR:   Q92HV7
- GeneID:   927740
- GenomeReviews:   AE006914_GR
- KEGG:   rco:RC0663
- NMPDR:   fig|272944.1.peg.663
- OMA:   YGETVVL
- ProtClustDB:   PRK11824
- BioCyc:   RCON272944:RC0663-MONOMER
- BRENDA:   2.7.7.8
- GO:   GO:0005739
- HAMAP:   MF_01595
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:1.10.10.400
- PANTHER:   PTHR11252
- PIRSF:   PIRSF005499
- SMART:   SM00322
- SMART:   SM00316
- TIGRFAMs:   TIGR03591

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1; SSF46915 3_ExoRNase; SSF55666 3_ExoRNase; SSF50249 Nucleic_acid_OB; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.8

Molecular weight: Translated: 82119; Mature: 82119

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: PS50084 KH_TYPE_1; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFNEITKSVTWNGQVLELSTGKIARQADGAVTVKMGNSVLLCTAVVANKAKEGIGFLPLT
CCCHHHCCEECCCEEEEECCCCHHHCCCCEEEEEECCCEEEEHHHHHHHHHCCCCEEEEE
INYREMAYAAGKIPGGFFKHEGKASDREVLVSRLIDRPIRPLFHPAFVNETHVTCSVLSY
ECHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHCHHHCCCCCEEEEEEEC
DPETPVDILAIIGASAALSLSPAPYLEIVAASKVGLINGEFVLNPTLALLKTSQLDLVVA
CCCCCHHHHHHHCCCCEEECCCCCCEEEEECCCEEEECCCEEECCHHEEEEECCEEEEEE
GTSDSVMMVESEAHLLSEEQMLEAVKFGFESFQPVIKIIKELAEEAKKPKLEMQALYPAS
CCCCCEEEEECHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHH
LKKEIEKLFVKEIEQAFAIKSKQERSTNLDLIPEKVLTHFVSDIENKKYSNYQIESALKA
HHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHH
IESDILRNEILEKNRRIDGRSTTDIRQIACEIGLLPSAHGSALFTRGETQSLVSTTFGTS
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCEEEECCCHHHHHHHHHCCC
LDEQIVDSLEGEYKERFMLNYIFPPYSVNEAMPMKAPSRREVGHGKLAWRAINPILPNKV
HHHHHHHHHCCHHHHHEEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCC
QFPYSIRVVAETTESNGSSSMATVCGSSLALMYAGVPIKAPVAGIAMGLVKEGKNFAVLS
CCCEEEEEEEEECCCCCCCHHHHHHCCHHEEHCCCCCCCCCHHHHHHHHHHCCCCEEEHH
DILGDEDYFGDMDFKVAGTSEGITALQMDIKISGVDFKIMKVALEQARLGRLHILEQMNK
HHHCCCCCCCCCCEEEECCCCCCEEEEEEEEEECCCHHHHHHHHHHHHCCHHHHHHHHHH
VISKPNNELSKNAPSTTTIKIDKDKIRDIIGPGGKIIKEICETSGAKIDISDDGTVSVYA
HHCCCCCHHHCCCCCCEEEEECHHHHHHHHCCCHHHHHHHHHCCCCEEEECCCCEEEEEE
SDRDKLKVALDKIKAIVVEPEIGEIFNGTVVKVLDSGAFINYVGNKDGFVHISEVSGERI
CCCHHHHHHHHHEEEEEECCCCCHHHCCEEEEEECCCCEEEEECCCCCEEEEECCCCHHH
ETVSSVLKQGDIVKVKLIGFDNKGKAKLTIKNADKDKSSNNTKPKTNVNNTNKDNSEPEQ
HHHHHHHHCCCEEEEEEEEECCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHH
RRDSSKKRAWNEDNNAETAEVITERKYFN
HHHHHHHHCCCCCCCCHHHHHHHHHCCCC
>Mature Secondary Structure
MFNEITKSVTWNGQVLELSTGKIARQADGAVTVKMGNSVLLCTAVVANKAKEGIGFLPLT
CCCHHHCCEECCCEEEEECCCCHHHCCCCEEEEEECCCEEEEHHHHHHHHHCCCCEEEEE
INYREMAYAAGKIPGGFFKHEGKASDREVLVSRLIDRPIRPLFHPAFVNETHVTCSVLSY
ECHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHCHHHCCCCCEEEEEEEC
DPETPVDILAIIGASAALSLSPAPYLEIVAASKVGLINGEFVLNPTLALLKTSQLDLVVA
CCCCCHHHHHHHCCCCEEECCCCCCEEEEECCCEEEECCCEEECCHHEEEEECCEEEEEE
GTSDSVMMVESEAHLLSEEQMLEAVKFGFESFQPVIKIIKELAEEAKKPKLEMQALYPAS
CCCCCEEEEECHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHH
LKKEIEKLFVKEIEQAFAIKSKQERSTNLDLIPEKVLTHFVSDIENKKYSNYQIESALKA
HHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHH
IESDILRNEILEKNRRIDGRSTTDIRQIACEIGLLPSAHGSALFTRGETQSLVSTTFGTS
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCEEEECCCHHHHHHHHHCCC
LDEQIVDSLEGEYKERFMLNYIFPPYSVNEAMPMKAPSRREVGHGKLAWRAINPILPNKV
HHHHHHHHHCCHHHHHEEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCC
QFPYSIRVVAETTESNGSSSMATVCGSSLALMYAGVPIKAPVAGIAMGLVKEGKNFAVLS
CCCEEEEEEEEECCCCCCCHHHHHHCCHHEEHCCCCCCCCCHHHHHHHHHHCCCCEEEHH
DILGDEDYFGDMDFKVAGTSEGITALQMDIKISGVDFKIMKVALEQARLGRLHILEQMNK
HHHCCCCCCCCCCEEEECCCCCCEEEEEEEEEECCCHHHHHHHHHHHHCCHHHHHHHHHH
VISKPNNELSKNAPSTTTIKIDKDKIRDIIGPGGKIIKEICETSGAKIDISDDGTVSVYA
HHCCCCCHHHCCCCCCEEEEECHHHHHHHHCCCHHHHHHHHHCCCCEEEECCCCEEEEEE
SDRDKLKVALDKIKAIVVEPEIGEIFNGTVVKVLDSGAFINYVGNKDGFVHISEVSGERI
CCCHHHHHHHHHEEEEEECCCCCHHHCCEEEEEECCCCEEEEECCCCCEEEEECCCCHHH
ETVSSVLKQGDIVKVKLIGFDNKGKAKLTIKNADKDKSSNNTKPKTNVNNTNKDNSEPEQ
HHHHHHHHCCCEEEEEEEEECCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHH
RRDSSKKRAWNEDNNAETAEVITERKYFN
HHHHHHHHCCCCCCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11557893