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The map label for this gene is folD

Identifier: 15892559

GI number: 15892559

Start: 620453

End: 621319

Strand: Reverse

Name: folD

Synonym: RC0636

Alternate gene names: 15892559

Gene position: 621319-620453 (Counterclockwise)

Preceding gene: 15892560

Following gene: 15892555

Centisome position: 48.97

GC content: 33.22

Gene sequence:

>867_bases
GTGAATAATATAATTGACGGTAAAGCATTAGCCAATGAGATACTAGCAGACTTAAAGCTAGAAATTCAAGAACTTACAAG
CCAAACCAACGCTTCACCGAAGCTTGCTATAGTCCTAGTAGGCGATAATCCGGCAAGCATTATTTATGTAAGACACAAAA
TAAAAAATGCACATAAAATAGGAATTTATACTTTATTAATAAATCTATCTGCTACTATTCATACTAATGATTTAATATCA
AAAATCAATGAGTTAAACCTTGATAACGAGATTTCAGGAATTATAGTTCAGCTTCCTTTGCCAAGCTCCATAGATAAAAA
TAAAATTTTATCTGCAGTATCACCTTCTAAAGATATCGATGGTTTCCACCCTTTAAATGTCGGTTACTTACATAGCGGAA
TTAGTCAAGGCTTTATTCCCTGCACTGCTCTTGGTTGTCTTGCAGCTATAAAAAAATACGAACCTAATTTAACCGGTAAA
AACGTTGTAATTATTGGACGCTCAAATATAGTAGGCAAACCTTTAAGTGCTTTACTGTTAAAAGAAAATTGCAGCGTTAC
TATTTGTCATTCTAAGACACACAATTTACGCTCTATTACTTCTAAAGCCGATATTGTCGTTGCTGCAATAGGCTCACCTT
TAAAATTAACTGCAGAATATTTTAATCCTAAATCTATAGTAATTGATGTAGGCATTAATAGAATTAGCAGCAATAAAATT
ATCGGTGATGTTGATTTTGAAAATGTTCAGTCTAAAGTACAATATATTACTCCCGTCCCAGGCGGCATCGGACCGATGAC
TATTGCGTTTTTACTTAAAAATACGGTTAAGGCATTTAAGGATTCCTTATATACTCTTGATACGTAA

Upstream 100 bases:

>100_bases
CAATCTCATGAAACAATAACAAACTCCTGAGATTGCTTCGTCAATTACTTACGTAATTTTCTTGCAATGACGTTAAAACT
GAACGGAGACAAAAATAAGA

Downstream 100 bases:

>100_bases
AAAGTAGATAGACGACGCCAATTTTTCAAATTAAACGAGTATCTTATAAACATTTTCAAGTTCTACATAATGTCTTACAT
TATCTTGCATATATTCTAAA

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase

Number of amino acids: Translated: 288; Mature: 288

Protein sequence:

>288_residues
MNNIIDGKALANEILADLKLEIQELTSQTNASPKLAIVLVGDNPASIIYVRHKIKNAHKIGIYTLLINLSATIHTNDLIS
KINELNLDNEISGIIVQLPLPSSIDKNKILSAVSPSKDIDGFHPLNVGYLHSGISQGFIPCTALGCLAAIKKYEPNLTGK
NVVIIGRSNIVGKPLSALLLKENCSVTICHSKTHNLRSITSKADIVVAAIGSPLKLTAEYFNPKSIVIDVGINRISSNKI
IGDVDFENVQSKVQYITPVPGGIGPMTIAFLLKNTVKAFKDSLYTLDT

Sequences:

>Translated_288_residues
MNNIIDGKALANEILADLKLEIQELTSQTNASPKLAIVLVGDNPASIIYVRHKIKNAHKIGIYTLLINLSATIHTNDLIS
KINELNLDNEISGIIVQLPLPSSIDKNKILSAVSPSKDIDGFHPLNVGYLHSGISQGFIPCTALGCLAAIKKYEPNLTGK
NVVIIGRSNIVGKPLSALLLKENCSVTICHSKTHNLRSITSKADIVVAAIGSPLKLTAEYFNPKSIVIDVGINRISSNKI
IGDVDFENVQSKVQYITPVPGGIGPMTIAFLLKNTVKAFKDSLYTLDT
>Mature_288_residues
MNNIIDGKALANEILADLKLEIQELTSQTNASPKLAIVLVGDNPASIIYVRHKIKNAHKIGIYTLLINLSATIHTNDLIS
KINELNLDNEISGIIVQLPLPSSIDKNKILSAVSPSKDIDGFHPLNVGYLHSGISQGFIPCTALGCLAAIKKYEPNLTGK
NVVIIGRSNIVGKPLSALLLKENCSVTICHSKTHNLRSITSKADIVVAAIGSPLKLTAEYFNPKSIVIDVGINRISSNKI
IGDVDFENVQSKVQYITPVPGGIGPMTIAFLLKNTVKAFKDSLYTLDT

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family

Homologues:

Organism=Homo sapiens, GI222136639, Length=291, Percent_Identity=40.2061855670103, Blast_Score=219, Evalue=2e-57,
Organism=Homo sapiens, GI222418558, Length=300, Percent_Identity=41.3333333333333, Blast_Score=214, Evalue=9e-56,
Organism=Homo sapiens, GI94721354, Length=296, Percent_Identity=42.9054054054054, Blast_Score=212, Evalue=4e-55,
Organism=Homo sapiens, GI36796743, Length=226, Percent_Identity=25.2212389380531, Blast_Score=69, Evalue=4e-12,
Organism=Escherichia coli, GI1786741, Length=275, Percent_Identity=43.6363636363636, Blast_Score=242, Evalue=2e-65,
Organism=Caenorhabditis elegans, GI17568735, Length=288, Percent_Identity=40.9722222222222, Blast_Score=202, Evalue=2e-52,
Organism=Saccharomyces cerevisiae, GI6319558, Length=289, Percent_Identity=41.8685121107266, Blast_Score=229, Evalue=3e-61,
Organism=Saccharomyces cerevisiae, GI6321643, Length=297, Percent_Identity=40.4040404040404, Blast_Score=212, Evalue=5e-56,
Organism=Drosophila melanogaster, GI62472483, Length=284, Percent_Identity=43.6619718309859, Blast_Score=227, Evalue=6e-60,
Organism=Drosophila melanogaster, GI45551871, Length=284, Percent_Identity=43.6619718309859, Blast_Score=227, Evalue=6e-60,
Organism=Drosophila melanogaster, GI24645718, Length=284, Percent_Identity=43.6619718309859, Blast_Score=226, Evalue=2e-59,
Organism=Drosophila melanogaster, GI17137370, Length=284, Percent_Identity=43.6619718309859, Blast_Score=226, Evalue=2e-59,
Organism=Drosophila melanogaster, GI17136818, Length=298, Percent_Identity=38.255033557047, Blast_Score=201, Evalue=4e-52,
Organism=Drosophila melanogaster, GI17136816, Length=298, Percent_Identity=38.255033557047, Blast_Score=201, Evalue=5e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FOLD_RICAE (C3PNI9)

Other databases:

- EMBL:   CP001612
- RefSeq:   YP_002845242.1
- ProteinModelPortal:   C3PNI9
- SMR:   C3PNI9
- GeneID:   7815007
- GenomeReviews:   CP001612_GR
- KEGG:   raf:RAF_ORF0590
- OMA:   TYVRNKQ
- ProtClustDB:   PRK14171
- GO:   GO:0005488
- HAMAP:   MF_01576
- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631
- Gene3D:   G3DSA:3.40.50.720
- PRINTS:   PR00085

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C

EC number: =1.5.1.5; =3.5.4.9

Molecular weight: Translated: 30996; Mature: 30996

Theoretical pI: Translated: 9.09; Mature: 9.09

Prosite motif: PS00766 THF_DHG_CYH_1; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNIIDGKALANEILADLKLEIQELTSQTNASPKLAIVLVGDNPASIIYVRHKIKNAHKI
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEEEECCCCCCEE
GIYTLLINLSATIHTNDLISKINELNLDNEISGIIVQLPLPSSIDKNKILSAVSPSKDID
EEEEEEEEEEEEEECHHHHHHHHHCCCCCCCCCEEEEECCCCCCCHHHHHHHCCCCCCCC
GFHPLNVGYLHSGISQGFIPCTALGCLAAIKKYEPNLTGKNVVIIGRSNIVGKPLSALLL
CCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCHHHHEE
KENCSVTICHSKTHNLRSITSKADIVVAAIGSPLKLTAEYFNPKSIVIDVGINRISSNKI
ECCCCEEEEECCCCCHHHHCCCCCEEEEECCCCEEEEEECCCCCEEEEEECCCEECCCCE
IGDVDFENVQSKVQYITPVPGGIGPMTIAFLLKNTVKAFKDSLYTLDT
EECCCHHHHHHHEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCEECCC
>Mature Secondary Structure
MNNIIDGKALANEILADLKLEIQELTSQTNASPKLAIVLVGDNPASIIYVRHKIKNAHKI
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEEEECCCCCCEE
GIYTLLINLSATIHTNDLISKINELNLDNEISGIIVQLPLPSSIDKNKILSAVSPSKDID
EEEEEEEEEEEEEECHHHHHHHHHCCCCCCCCCEEEEECCCCCCCHHHHHHHCCCCCCCC
GFHPLNVGYLHSGISQGFIPCTALGCLAAIKKYEPNLTGKNVVIIGRSNIVGKPLSALLL
CCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCHHHHEE
KENCSVTICHSKTHNLRSITSKADIVVAAIGSPLKLTAEYFNPKSIVIDVGINRISSNKI
ECCCCEEEEECCCCCHHHHCCCCCEEEEECCCCEEEEEECCCCCEEEEEECCCEECCCCE
IGDVDFENVQSKVQYITPVPGGIGPMTIAFLLKNTVKAFKDSLYTLDT
EECCCHHHHHHHEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA