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The map label for this gene is mdh [H]

Identifier: 15892443

GI number: 15892443

Start: 509332

End: 510276

Strand: Reverse

Name: mdh [H]

Synonym: RC0520

Alternate gene names: 15892443

Gene position: 510276-509332 (Counterclockwise)

Preceding gene: 15892445

Following gene: 15892442

Centisome position: 40.22

GC content: 38.1

Gene sequence:

>945_bases
ATGAAACAAAACGCAAAAATCTCATTAATAGGTAGCGGTAACATAGGTGGTACACTTGCTCACTTAATTAGCCTTAGAGA
ACTCGGCGATATCGTGTTATTTGATGTGACTGAAGGAGTACCGCAAGGAAAAGCATTGGATCTCATGCAAGCCGGTACAA
TAGCCGGGTCTGATATCAAAATAAAAGGCACAAACGACTATAAAGATATTGAAGGATCTGATGCCATAATTATTACTGCC
GGTTTACCAAGAAAACCAGGGATGAGTAGAGAAGATTTAATTAGCATTAACACCGGTATTATGAAAACGGTTGCAGCAAA
TGTTAAAAAATACGCTCCTGATGCCTTTGTAATAGTAATTACCAATCCACTTGATGTTATGGTATATGTCATGCTTAAAG
AAAGCGGATTGCCTCACAATAAAGTAATCGGTATGGCAGGAGTACTTGACTCATCAAGATTTAATCTTTTTCTTGCTGAA
GAGTTTAAAGTATCGGTTAACAATGTTAATAGCATGGTTCTCGGCGGTCACGGCGATGCTATGGTACCTCTTGCAAGATA
TTCTACAATATCAGGCGTACCGATACCTGACTTGATAAAAATGGGCTTATCAAGTAATGAAAATATTGAAAAAATAATCG
ATCGTACTAGAAACGGCGGCGGTGAGATTGTTGCATTACTTAAAACAGGTTCTGCTTATTATGCTCCCGCAGCCTCAGCA
ATAGAAATGCTTGAGTCATATTTAAAAGATAAACGTCAGATTCTAACTTGTGCCGCTCATTTACAAGGTGAGTACGGCGT
ACATGATTTATATGTCGGAGTGCCTATTATGATCGGTAAAGAAGGTGTTCTAAGAGTAATAGAACTGCAGTTAACCGCAG
AGGAAAAAGCACTATTTGATAAATCAGTCGAGGGAGTTAAAAAACTCATTGAGACGATAAAATAA

Upstream 100 bases:

>100_bases
GAATTAACCAATTAACAATGTAATATAATTTAAAAATTCTTTTTTCTTGAATCTATTTGTAATATAGTAATAGGCATATT
TAATATTTGTAGAGGGAGTA

Downstream 100 bases:

>100_bases
ATGATAAAATTATATTATTGAAGCACAAATGCAACAAGATTTAAAATATTGAATTAATACTAATTATTAGTATTGTTAAT
TGAACATAAATGGTATTACA

Product: malate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MKQNAKISLIGSGNIGGTLAHLISLRELGDIVLFDVTEGVPQGKALDLMQAGTIAGSDIKIKGTNDYKDIEGSDAIIITA
GLPRKPGMSREDLISINTGIMKTVAANVKKYAPDAFVIVITNPLDVMVYVMLKESGLPHNKVIGMAGVLDSSRFNLFLAE
EFKVSVNNVNSMVLGGHGDAMVPLARYSTISGVPIPDLIKMGLSSNENIEKIIDRTRNGGGEIVALLKTGSAYYAPAASA
IEMLESYLKDKRQILTCAAHLQGEYGVHDLYVGVPIMIGKEGVLRVIELQLTAEEKALFDKSVEGVKKLIETIK

Sequences:

>Translated_314_residues
MKQNAKISLIGSGNIGGTLAHLISLRELGDIVLFDVTEGVPQGKALDLMQAGTIAGSDIKIKGTNDYKDIEGSDAIIITA
GLPRKPGMSREDLISINTGIMKTVAANVKKYAPDAFVIVITNPLDVMVYVMLKESGLPHNKVIGMAGVLDSSRFNLFLAE
EFKVSVNNVNSMVLGGHGDAMVPLARYSTISGVPIPDLIKMGLSSNENIEKIIDRTRNGGGEIVALLKTGSAYYAPAASA
IEMLESYLKDKRQILTCAAHLQGEYGVHDLYVGVPIMIGKEGVLRVIELQLTAEEKALFDKSVEGVKKLIETIK
>Mature_314_residues
MKQNAKISLIGSGNIGGTLAHLISLRELGDIVLFDVTEGVPQGKALDLMQAGTIAGSDIKIKGTNDYKDIEGSDAIIITA
GLPRKPGMSREDLISINTGIMKTVAANVKKYAPDAFVIVITNPLDVMVYVMLKESGLPHNKVIGMAGVLDSSRFNLFLAE
EFKVSVNNVNSMVLGGHGDAMVPLARYSTISGVPIPDLIKMGLSSNENIEKIIDRTRNGGGEIVALLKTGSAYYAPAASA
IEMLESYLKDKRQILTCAAHLQGEYGVHDLYVGVPIMIGKEGVLRVIELQLTAEEKALFDKSVEGVKKLIETIK

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate [H]

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 3 family [H]

Homologues:

Organism=Homo sapiens, GI47059044, Length=316, Percent_Identity=33.5443037974684, Blast_Score=176, Evalue=2e-44,
Organism=Homo sapiens, GI221136809, Length=316, Percent_Identity=33.5443037974684, Blast_Score=176, Evalue=2e-44,
Organism=Homo sapiens, GI9257228, Length=305, Percent_Identity=33.1147540983607, Blast_Score=166, Evalue=2e-41,
Organism=Homo sapiens, GI4504973, Length=305, Percent_Identity=33.1147540983607, Blast_Score=166, Evalue=2e-41,
Organism=Homo sapiens, GI5031857, Length=319, Percent_Identity=32.2884012539185, Blast_Score=157, Evalue=8e-39,
Organism=Homo sapiens, GI260099723, Length=319, Percent_Identity=32.2884012539185, Blast_Score=157, Evalue=1e-38,
Organism=Homo sapiens, GI15082234, Length=319, Percent_Identity=29.4670846394984, Blast_Score=156, Evalue=2e-38,
Organism=Homo sapiens, GI291575128, Length=315, Percent_Identity=30.1587301587302, Blast_Score=150, Evalue=1e-36,
Organism=Homo sapiens, GI4557032, Length=315, Percent_Identity=30.1587301587302, Blast_Score=150, Evalue=1e-36,
Organism=Homo sapiens, GI260099727, Length=198, Percent_Identity=36.8686868686869, Blast_Score=123, Evalue=2e-28,
Organism=Homo sapiens, GI260099725, Length=201, Percent_Identity=37.3134328358209, Blast_Score=123, Evalue=2e-28,
Organism=Homo sapiens, GI21735621, Length=295, Percent_Identity=29.8305084745763, Blast_Score=99, Evalue=7e-21,
Organism=Homo sapiens, GI207028494, Length=202, Percent_Identity=29.2079207920792, Blast_Score=96, Evalue=5e-20,
Organism=Homo sapiens, GI103472011, Length=314, Percent_Identity=22.2929936305732, Blast_Score=73, Evalue=3e-13,
Organism=Escherichia coli, GI1789632, Length=293, Percent_Identity=31.740614334471, Blast_Score=105, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI17535107, Length=272, Percent_Identity=29.0441176470588, Blast_Score=135, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI17554310, Length=321, Percent_Identity=28.6604361370716, Blast_Score=99, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6322765, Length=299, Percent_Identity=28.0936454849498, Blast_Score=87, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6320125, Length=275, Percent_Identity=27.2727272727273, Blast_Score=79, Evalue=9e-16,
Organism=Saccharomyces cerevisiae, GI6324446, Length=249, Percent_Identity=25.7028112449799, Blast_Score=69, Evalue=9e-13,
Organism=Drosophila melanogaster, GI17136226, Length=302, Percent_Identity=29.4701986754967, Blast_Score=139, Evalue=2e-33,
Organism=Drosophila melanogaster, GI45550422, Length=300, Percent_Identity=28, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24647881, Length=322, Percent_Identity=29.5031055900621, Blast_Score=109, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24663599, Length=291, Percent_Identity=29.553264604811, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24663595, Length=283, Percent_Identity=26.5017667844523, Blast_Score=79, Evalue=4e-15,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR011275
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N [H]

EC number: =1.1.1.37 [H]

Molecular weight: Translated: 33639; Mature: 33639

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQNAKISLIGSGNIGGTLAHLISLRELGDIVLFDVTEGVPQGKALDLMQAGTIAGSDIK
CCCCCEEEEEECCCCCHHHHHHHHHHHHCCEEEEEECCCCCCCCHHHHHHCCCCCCCCEE
IKGTNDYKDIEGSDAIIITAGLPRKPGMSREDLISINTGIMKTVAANVKKYAPDAFVIVI
EECCCCCCCCCCCCEEEEEECCCCCCCCCHHHEEEECHHHHHHHHHHHHHHCCCEEEEEE
TNPLDVMVYVMLKESGLPHNKVIGMAGVLDSSRFNLFLAEEFKVSVNNVNSMVLGGHGDA
ECCHHHEEEEEECCCCCCCHHEEEEHEEECCCCEEEEEEEEEEEEECCCCEEEECCCCCC
MVPLARYSTISGVPIPDLIKMGLSSNENIEKIIDRTRNGGGEIVALLKTGSAYYAPAASA
EEEHHHHCCCCCCCCHHHHHHCCCCCCCHHHHHHHCCCCCCEEEEEEECCCEEECCHHHH
IEMLESYLKDKRQILTCAAHLQGEYGVHDLYVGVPIMIGKEGVLRVIELQLTAEEKALFD
HHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCEEEECCCCCEEEEEEEEECHHHHHHH
KSVEGVKKLIETIK
HHHHHHHHHHHHCC
>Mature Secondary Structure
MKQNAKISLIGSGNIGGTLAHLISLRELGDIVLFDVTEGVPQGKALDLMQAGTIAGSDIK
CCCCCEEEEEECCCCCHHHHHHHHHHHHCCEEEEEECCCCCCCCHHHHHHCCCCCCCCEE
IKGTNDYKDIEGSDAIIITAGLPRKPGMSREDLISINTGIMKTVAANVKKYAPDAFVIVI
EECCCCCCCCCCCCEEEEEECCCCCCCCCHHHEEEECHHHHHHHHHHHHHHCCCEEEEEE
TNPLDVMVYVMLKESGLPHNKVIGMAGVLDSSRFNLFLAEEFKVSVNNVNSMVLGGHGDA
ECCHHHEEEEEECCCCCCCHHEEEEHEEECCCCEEEEEEEEEEEEECCCCEEEECCCCCC
MVPLARYSTISGVPIPDLIKMGLSSNENIEKIIDRTRNGGGEIVALLKTGSAYYAPAASA
EEEHHHHCCCCCCCCHHHHHHCCCCCCCHHHHHHHCCCCCCEEEEEEECCCEEECCHHHH
IEMLESYLKDKRQILTCAAHLQGEYGVHDLYVGVPIMIGKEGVLRVIELQLTAEEKALFD
HHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCEEEECCCCCEEEEEEEEECHHHHHHH
KSVEGVKKLIETIK
HHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA