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The map label for this gene is sucB [H]

Identifier: 15892149

GI number: 15892149

Start: 232626

End: 233813

Strand: Reverse

Name: sucB [H]

Synonym: RC0226

Alternate gene names: 15892149

Gene position: 233813-232626 (Counterclockwise)

Preceding gene: 15892150

Following gene: 15892148

Centisome position: 18.43

GC content: 37.21

Gene sequence:

>1188_bases
ATGAGAGTTAAAATTATAGTACCGTCGCTTGGGGAGTCCATAACAGAAGCAACTATTGCCAAATGGTATAAGAAACAAGG
CGATTCGGTTAAAACCGATGAATTACTGTTAGAAATCGAAACCGAAAAAGTGACTTTAGAAGTTAATGCTCCTTGCAATG
GTACTATAGGTAAAATATCGAAAACTGAAGGTGCAAATGTAGCAGTTGGCGAAGAAATAGGCGAAATAAATGAAGGAGCA
TCTGCAAATACTGCCGGTACTAATAATGAATCTGCTAAAGCTCAAGCAGTAACACAACCTACTTCAGAAAAACCTGCCGT
GGCTAATAATACTCTTGCTCCTTCTGTACAAAAATTAGTGACCGAAAATAAGCTTGACCCCAATAATATAAAAGGAACAG
GTAGAGACGGTAGAATTACCAAAGGTGACGTGCTTGCAACAATAAACACTACGACTACTTCTGCTCCTGCAATAAGTAAA
TCTAATGAAGAAAGAGTGCAGCGTGTTCGTATGTCACGCTTGCGTAAAACTATTGCACAGCGTTTAAAAGATTCACAAAA
TACAGCAGCTATTTTGACTACTTTTAATGAAATCGATATGTCAAAAGTAATTGCTTTGCGTAATCAATATAAAGAAGAGT
TTGAGAAAAAGCACGCTGTGAAACTTGGGTTTATGTCTTTCTTTGTTAAAGCAACAATTGAAGCTTTAAAGCTTATTCCG
TCGGTAAATGCTGAAATAGATGGCGATGATTTAGTATATAAAAATTACTATGATATAGGTGTAGCTGTCGGAACAGAGCA
AGGGCTTGTTGTACCGGTTGTTAGGGATGCCGATAAAATGGGTTTTGCCGAAGTGGAGAAAACTATAGGAATTTTGGCTA
AACAAGCTCGTGAGGGTAAGCTTTCTATGGCTGATTTGTCAGGTGGGACATTCTCGATTTCTAACGGAGGCGTATATGGC
TCATTATTATCTACACCGATTATTAATCCTCCTCAATCAGGTATTCTAGGCTTACATAAAACCGAGGAAAGAGCTGTAGT
TATAGACGGTAAAATTGAAATACGTCCGATGATGTATATAGCTTTATCTTACGATCATCGTATAATTGACGGGAAAGAAG
GAGTATCGTTCTTGGTAAAAATTAAGCAGCTTATTGAGAATCCTGAAAAGTTATTGTTAAATTTGTAA

Upstream 100 bases:

>100_bases
ATTTGCCTGAGATTGCTTCGTCGACCCTTATAGCGTCTTCTCGCAATGACGATAAAACTATACAGGGCGATGCTATACAA
ATATAGGAGTAAAATAAATT

Downstream 100 bases:

>100_bases
AAAATTGTCATTGCGAGCAGCCGTAGGCTGCGTGGCAATCTCATGAAATCATAACAAACTCCTGAGATTGCTTCGTCAAT
TACTTCGTAATTTTCCTCAC

Product: dihydrolipoamide succinyltransferase

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 395; Mature: 395

Protein sequence:

>395_residues
MRVKIIVPSLGESITEATIAKWYKKQGDSVKTDELLLEIETEKVTLEVNAPCNGTIGKISKTEGANVAVGEEIGEINEGA
SANTAGTNNESAKAQAVTQPTSEKPAVANNTLAPSVQKLVTENKLDPNNIKGTGRDGRITKGDVLATINTTTTSAPAISK
SNEERVQRVRMSRLRKTIAQRLKDSQNTAAILTTFNEIDMSKVIALRNQYKEEFEKKHAVKLGFMSFFVKATIEALKLIP
SVNAEIDGDDLVYKNYYDIGVAVGTEQGLVVPVVRDADKMGFAEVEKTIGILAKQAREGKLSMADLSGGTFSISNGGVYG
SLLSTPIINPPQSGILGLHKTEERAVVIDGKIEIRPMMYIALSYDHRIIDGKEGVSFLVKIKQLIENPEKLLLNL

Sequences:

>Translated_395_residues
MRVKIIVPSLGESITEATIAKWYKKQGDSVKTDELLLEIETEKVTLEVNAPCNGTIGKISKTEGANVAVGEEIGEINEGA
SANTAGTNNESAKAQAVTQPTSEKPAVANNTLAPSVQKLVTENKLDPNNIKGTGRDGRITKGDVLATINTTTTSAPAISK
SNEERVQRVRMSRLRKTIAQRLKDSQNTAAILTTFNEIDMSKVIALRNQYKEEFEKKHAVKLGFMSFFVKATIEALKLIP
SVNAEIDGDDLVYKNYYDIGVAVGTEQGLVVPVVRDADKMGFAEVEKTIGILAKQAREGKLSMADLSGGTFSISNGGVYG
SLLSTPIINPPQSGILGLHKTEERAVVIDGKIEIRPMMYIALSYDHRIIDGKEGVSFLVKIKQLIENPEKLLLNL
>Mature_395_residues
MRVKIIVPSLGESITEATIAKWYKKQGDSVKTDELLLEIETEKVTLEVNAPCNGTIGKISKTEGANVAVGEEIGEINEGA
SANTAGTNNESAKAQAVTQPTSEKPAVANNTLAPSVQKLVTENKLDPNNIKGTGRDGRITKGDVLATINTTTTSAPAISK
SNEERVQRVRMSRLRKTIAQRLKDSQNTAAILTTFNEIDMSKVIALRNQYKEEFEKKHAVKLGFMSFFVKATIEALKLIP
SVNAEIDGDDLVYKNYYDIGVAVGTEQGLVVPVVRDADKMGFAEVEKTIGILAKQAREGKLSMADLSGGTFSISNGGVYG
SLLSTPIINPPQSGILGLHKTEERAVVIDGKIEIRPMMYIALSYDHRIIDGKEGVSFLVKIKQLIENPEKLLLNL

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components:2- oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2)

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI19923748, Length=231, Percent_Identity=58.4415584415584, Blast_Score=288, Evalue=9e-78,
Organism=Homo sapiens, GI31711992, Length=433, Percent_Identity=29.3302540415704, Blast_Score=178, Evalue=9e-45,
Organism=Homo sapiens, GI203098753, Length=451, Percent_Identity=27.4944567627494, Blast_Score=163, Evalue=3e-40,
Organism=Homo sapiens, GI203098816, Length=451, Percent_Identity=27.4944567627494, Blast_Score=162, Evalue=7e-40,
Organism=Homo sapiens, GI110671329, Length=428, Percent_Identity=27.803738317757, Blast_Score=155, Evalue=5e-38,
Organism=Homo sapiens, GI260898739, Length=160, Percent_Identity=35.625, Blast_Score=102, Evalue=7e-22,
Organism=Escherichia coli, GI1786946, Length=403, Percent_Identity=49.3796526054591, Blast_Score=402, Evalue=1e-113,
Organism=Escherichia coli, GI1786305, Length=424, Percent_Identity=29.9528301886792, Blast_Score=163, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI25146366, Length=401, Percent_Identity=43.8902743142145, Blast_Score=327, Evalue=8e-90,
Organism=Caenorhabditis elegans, GI17560088, Length=434, Percent_Identity=31.1059907834101, Blast_Score=177, Evalue=7e-45,
Organism=Caenorhabditis elegans, GI17537937, Length=426, Percent_Identity=26.7605633802817, Blast_Score=155, Evalue=3e-38,
Organism=Caenorhabditis elegans, GI17538894, Length=305, Percent_Identity=29.5081967213115, Blast_Score=122, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6320352, Length=402, Percent_Identity=44.0298507462687, Blast_Score=328, Evalue=1e-90,
Organism=Saccharomyces cerevisiae, GI6324258, Length=449, Percent_Identity=27.8396436525612, Blast_Score=149, Evalue=1e-36,
Organism=Drosophila melanogaster, GI18859875, Length=421, Percent_Identity=29.9287410926366, Blast_Score=177, Evalue=1e-44,
Organism=Drosophila melanogaster, GI24582497, Length=422, Percent_Identity=27.4881516587678, Blast_Score=129, Evalue=4e-30,
Organism=Drosophila melanogaster, GI20129315, Length=272, Percent_Identity=30.1470588235294, Blast_Score=119, Evalue=3e-27,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- InterPro:   IPR006255 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 42799; Mature: 42799

Theoretical pI: Translated: 7.58; Mature: 7.58

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVKIIVPSLGESITEATIAKWYKKQGDSVKTDELLLEIETEKVTLEVNAPCNGTIGKIS
CEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEEECCEEEEEECCCCCCCCCCCC
KTEGANVAVGEEIGEINEGASANTAGTNNESAKAQAVTQPTSEKPAVANNTLAPSVQKLV
CCCCCCEEECHHHHHCCCCCCCCCCCCCCCCCHHHEECCCCCCCCCCCCCCCCHHHHHHH
TENKLDPNNIKGTGRDGRITKGDVLATINTTTTSAPAISKSNEERVQRVRMSRLRKTIAQ
HHCCCCCCCCCCCCCCCCEECCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
RLKDSQNTAAILTTFNEIDMSKVIALRNQYKEEFEKKHAVKLGFMSFFVKATIEALKLIP
HHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
SVNAEIDGDDLVYKNYYDIGVAVGTEQGLVVPVVRDADKMGFAEVEKTIGILAKQAREGK
CCCCCCCCCCEEEEEEEEEEEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCC
LSMADLSGGTFSISNGGVYGSLLSTPIINPPQSGILGLHKTEERAVVIDGKIEIRPMMYI
EEEEECCCCEEEECCCCEEHHHHCCCCCCCCCCCCCCCEECCCCEEEECCEEEEEEEEEE
ALSYDHRIIDGKEGVSFLVKIKQLIENPEKLLLNL
EEECCCEEECCCCHHHHHHHHHHHHCCHHHHHCCC
>Mature Secondary Structure
MRVKIIVPSLGESITEATIAKWYKKQGDSVKTDELLLEIETEKVTLEVNAPCNGTIGKIS
CEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEEECCEEEEEECCCCCCCCCCCC
KTEGANVAVGEEIGEINEGASANTAGTNNESAKAQAVTQPTSEKPAVANNTLAPSVQKLV
CCCCCCEEECHHHHHCCCCCCCCCCCCCCCCCHHHEECCCCCCCCCCCCCCCCHHHHHHH
TENKLDPNNIKGTGRDGRITKGDVLATINTTTTSAPAISKSNEERVQRVRMSRLRKTIAQ
HHCCCCCCCCCCCCCCCCEECCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
RLKDSQNTAAILTTFNEIDMSKVIALRNQYKEEFEKKHAVKLGFMSFFVKATIEALKLIP
HHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
SVNAEIDGDDLVYKNYYDIGVAVGTEQGLVVPVVRDADKMGFAEVEKTIGILAKQAREGK
CCCCCCCCCCEEEEEEEEEEEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCC
LSMADLSGGTFSISNGGVYGSLLSTPIINPPQSGILGLHKTEERAVVIDGKIEIRPMMYI
EEEEECCCCEEEECCCCEEHHHHCCCCCCCCCCCCCCCEECCCCEEEECCEEEEEEEEEE
ALSYDHRIIDGKEGVSFLVKIKQLIENPEKLLLNL
EEECCCEEECCCCHHHHHHHHHHHHCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA