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The map label for this gene is gatA [H]

Identifier: 15892117

GI number: 15892117

Start: 201802

End: 203283

Strand: Reverse

Name: gatA [H]

Synonym: RC0194

Alternate gene names: 15892117

Gene position: 203283-201802 (Counterclockwise)

Preceding gene: 161723849

Following gene: 15892116

Centisome position: 16.02

GC content: 38.53

Gene sequence:

>1482_bases
ATGACGGAACTAAACAAACTAACAGTAGCAGACAGTATAAAAGGTCTAAAAAATAAAGATTTTACAAGTACGGAATTAAT
AGGTGCACATATTAAACAAATAGAGAAGCATAGAAACCTAAATGCTTATGTTACCGACACTTTTGACTTAGCTTTAAAAC
AGGCTGAAGCAGCCGATCAAAAGTATGCTCAAAATAACGCAAGAACCCTTGAGGGTATCCCGTTTGCCGCTAAAGATCTT
TTCTGCACGAAAGGAATTAGAACTACGGCATGTTCTAATATACTGAAAAATTTTATACCTAATTATGAATCAAGTGTTAC
ACAAAATATTTTTGATAAGGGCGGTGTGATGCTCGGCAAAACCAATATGGATGAGTTTGCTATGGGTTCAGCAAATATCA
CTAGTTGTTTTGGAAATGTAATTAGCCCTTGGAAAGCAAATGATGATAATGCTGATTTAGTTCCAGGCGGTTCTTCAGGC
GGTTCAGCTGCAGCAGTTAGCGGATTTATGGCATCTGCTGCTCTTGGTAGCGATACGGGCGGCTCGGTACGTCAACCTGC
AAGCTTTACGGGTTTAGTCGGTTTTAAACCGACATATGGTCGTTGCTCAAGATACGGAATGATATCGTTTGCTAGCTCCC
TTGATCAAGCAGGAATATTTACTAGAAGCGTTTTAGACAGTAGCATTATGCTTGAAGCGATGATGGGGTTTGACGAAAAA
GATTCTACTTCGATTAAAGCAGAAGTTCCAGAGCTACAATCGGCTATCGGAAGTTCTATGAAAAATATGAAGATAGGCGT
ACCTCTTAGCCTTGGTGAGGGCAGTATTATTGAACCTGATATTATGAAAATGTGGCAGGATACCATAGAACTACTTAAAA
ACGCCGGTGCTGAAATAGTTGATATTACTTTGCCGCATGCTAAATACGGTGTTGCTGTTTATTACGTAATAGCCCCGGCT
GAAGCGTCTTCAAATTTATCTAGATATGACGGCGTTAGATACGGTCTTCGAGTAGAACGTGAAAATATGACGCTTGACGA
AATGTATGAAATGACTAGATCAACTGGATTTGGGGAGGAAGTAAAACGTCGTATTATGATTGGAACATATGTGCTATCAT
CTAGTGGTATGGATGCATATTATCTAAAAGCTCAAAAAGTACGTCGTTTAGTTGCAAACGATTTTAATAATGCTTTTGCA
AAAGTTGATGCCATTTTATTACCGGCTGCCCCGACTGCAGCTTTTAAGATTGGTGAAAAACAAAATGATCCGACTATTAT
GTATTTAAACGATTTATTTACTATTCCTGCAAGTTTAGCCGGTTTACCTTGTGCATCGGTTCCTGCTGGATTATCGGCAC
GCGGTTTACCTCTTGGGATACAGATTATAGGTAAACAATTAGACGAATATAATGTTTTGAAAGTAGCATCGACAATTGAG
TCAGGTGTAAAACATATTAAATTTGAACCAAAGGGTTTTTAA

Upstream 100 bases:

>100_bases
CTGATTTATCAAGTGAGTTATTGGATAATGTAAGTGGAAATAGTGCTCAGCTGGCTAAAGAAGTAAAATATTTTATCACT
CCAAAGGTTGTTGAATAATT

Downstream 100 bases:

>100_bases
TTATGGCATATATTGAAGGTAATACGGGGAAATGGGAATATGTAATAGGGCTTGAAATCCATGCTCAAATTTCCTCAAAA
TCTAAGCTTTTTTCAGGAAG

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 493; Mature: 492

Protein sequence:

>493_residues
MTELNKLTVADSIKGLKNKDFTSTELIGAHIKQIEKHRNLNAYVTDTFDLALKQAEAADQKYAQNNARTLEGIPFAAKDL
FCTKGIRTTACSNILKNFIPNYESSVTQNIFDKGGVMLGKTNMDEFAMGSANITSCFGNVISPWKANDDNADLVPGGSSG
GSAAAVSGFMASAALGSDTGGSVRQPASFTGLVGFKPTYGRCSRYGMISFASSLDQAGIFTRSVLDSSIMLEAMMGFDEK
DSTSIKAEVPELQSAIGSSMKNMKIGVPLSLGEGSIIEPDIMKMWQDTIELLKNAGAEIVDITLPHAKYGVAVYYVIAPA
EASSNLSRYDGVRYGLRVERENMTLDEMYEMTRSTGFGEEVKRRIMIGTYVLSSSGMDAYYLKAQKVRRLVANDFNNAFA
KVDAILLPAAPTAAFKIGEKQNDPTIMYLNDLFTIPASLAGLPCASVPAGLSARGLPLGIQIIGKQLDEYNVLKVASTIE
SGVKHIKFEPKGF

Sequences:

>Translated_493_residues
MTELNKLTVADSIKGLKNKDFTSTELIGAHIKQIEKHRNLNAYVTDTFDLALKQAEAADQKYAQNNARTLEGIPFAAKDL
FCTKGIRTTACSNILKNFIPNYESSVTQNIFDKGGVMLGKTNMDEFAMGSANITSCFGNVISPWKANDDNADLVPGGSSG
GSAAAVSGFMASAALGSDTGGSVRQPASFTGLVGFKPTYGRCSRYGMISFASSLDQAGIFTRSVLDSSIMLEAMMGFDEK
DSTSIKAEVPELQSAIGSSMKNMKIGVPLSLGEGSIIEPDIMKMWQDTIELLKNAGAEIVDITLPHAKYGVAVYYVIAPA
EASSNLSRYDGVRYGLRVERENMTLDEMYEMTRSTGFGEEVKRRIMIGTYVLSSSGMDAYYLKAQKVRRLVANDFNNAFA
KVDAILLPAAPTAAFKIGEKQNDPTIMYLNDLFTIPASLAGLPCASVPAGLSARGLPLGIQIIGKQLDEYNVLKVASTIE
SGVKHIKFEPKGF
>Mature_492_residues
TELNKLTVADSIKGLKNKDFTSTELIGAHIKQIEKHRNLNAYVTDTFDLALKQAEAADQKYAQNNARTLEGIPFAAKDLF
CTKGIRTTACSNILKNFIPNYESSVTQNIFDKGGVMLGKTNMDEFAMGSANITSCFGNVISPWKANDDNADLVPGGSSGG
SAAAVSGFMASAALGSDTGGSVRQPASFTGLVGFKPTYGRCSRYGMISFASSLDQAGIFTRSVLDSSIMLEAMMGFDEKD
STSIKAEVPELQSAIGSSMKNMKIGVPLSLGEGSIIEPDIMKMWQDTIELLKNAGAEIVDITLPHAKYGVAVYYVIAPAE
ASSNLSRYDGVRYGLRVERENMTLDEMYEMTRSTGFGEEVKRRIMIGTYVLSSSGMDAYYLKAQKVRRLVANDFNNAFAK
VDAILLPAAPTAAFKIGEKQNDPTIMYLNDLFTIPASLAGLPCASVPAGLSARGLPLGIQIIGKQLDEYNVLKVASTIES
GVKHIKFEPKGF

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=491, Percent_Identity=40.7331975560082, Blast_Score=347, Evalue=1e-95,
Organism=Homo sapiens, GI195972892, Length=498, Percent_Identity=23.6947791164659, Blast_Score=115, Evalue=6e-26,
Organism=Caenorhabditis elegans, GI17543272, Length=461, Percent_Identity=39.4793926247288, Blast_Score=301, Evalue=4e-82,
Organism=Caenorhabditis elegans, GI17537465, Length=500, Percent_Identity=24, Blast_Score=114, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17556264, Length=291, Percent_Identity=25.7731958762887, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17556276, Length=308, Percent_Identity=26.2987012987013, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17556278, Length=308, Percent_Identity=26.2987012987013, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI71990152, Length=317, Percent_Identity=25.2365930599369, Blast_Score=69, Evalue=8e-12,
Organism=Saccharomyces cerevisiae, GI6323950, Length=428, Percent_Identity=34.8130841121495, Blast_Score=244, Evalue=2e-65,
Organism=Saccharomyces cerevisiae, GI6319685, Length=429, Percent_Identity=26.3403263403263, Blast_Score=103, Evalue=6e-23,
Organism=Drosophila melanogaster, GI24648113, Length=467, Percent_Identity=38.1156316916488, Blast_Score=298, Evalue=8e-81,
Organism=Drosophila melanogaster, GI24644968, Length=506, Percent_Identity=25.6916996047431, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24652985, Length=490, Percent_Identity=24.4897959183673, Blast_Score=109, Evalue=4e-24,
Organism=Drosophila melanogaster, GI19922090, Length=490, Percent_Identity=24.4897959183673, Blast_Score=109, Evalue=4e-24,
Organism=Drosophila melanogaster, GI24652981, Length=490, Percent_Identity=24.4897959183673, Blast_Score=109, Evalue=4e-24,
Organism=Drosophila melanogaster, GI24652983, Length=490, Percent_Identity=24.4897959183673, Blast_Score=109, Evalue=4e-24,
Organism=Drosophila melanogaster, GI161078093, Length=517, Percent_Identity=23.9845261121857, Blast_Score=90, Evalue=4e-18,
Organism=Drosophila melanogaster, GI45550774, Length=206, Percent_Identity=29.6116504854369, Blast_Score=82, Evalue=8e-16,
Organism=Drosophila melanogaster, GI21356731, Length=488, Percent_Identity=23.7704918032787, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24648435, Length=206, Percent_Identity=29.6116504854369, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24648437, Length=206, Percent_Identity=29.6116504854369, Blast_Score=82, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 53092; Mature: 52961

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTELNKLTVADSIKGLKNKDFTSTELIGAHIKQIEKHRNLNAYVTDTFDLALKQAEAADQ
CCCCHHEEHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHH
KYAQNNARTLEGIPFAAKDLFCTKGIRTTACSNILKNFIPNYESSVTQNIFDKGGVMLGK
HHHHCCCCEECCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEEC
TNMDEFAMGSANITSCFGNVISPWKANDDNADLVPGGSSGGSAAAVSGFMASAALGSDTG
CCCHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCC
GSVRQPASFTGLVGFKPTYGRCSRYGMISFASSLDQAGIFTRSVLDSSIMLEAMMGFDEK
CCCCCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCC
DSTSIKAEVPELQSAIGSSMKNMKIGVPLSLGEGSIIEPDIMKMWQDTIELLKNAGAEIV
CCCCEEEHHHHHHHHHHCCHHCEEECCEEECCCCCCCCHHHHHHHHHHHHHHHHCCCEEE
DITLPHAKYGVAVYYVIAPAEASSNLSRYDGVRYGLRVERENMTLDEMYEMTRSTGFGEE
EEECCCCCCCEEEEEEEECCCCCCCHHHHCCCEECEEEECCCCCHHHHHHHHHCCCCCHH
VKRRIMIGTYVLSSSGMDAYYLKAQKVRRLVANDFNNAFAKVDAILLPAAPTAAFKIGEK
HHHHHHHEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCHHEECCCC
QNDPTIMYLNDLFTIPASLAGLPCASVPAGLSARGLPLGIQIIGKQLDEYNVLKVASTIE
CCCCEEEEECHHHHHHHHHCCCCCCCCCCCCCCCCCCEEHHHHHHHCCCCHHHHHHHHHH
SGVKHIKFEPKGF
HHHHEEECCCCCC
>Mature Secondary Structure 
TELNKLTVADSIKGLKNKDFTSTELIGAHIKQIEKHRNLNAYVTDTFDLALKQAEAADQ
CCCHHEEHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHH
KYAQNNARTLEGIPFAAKDLFCTKGIRTTACSNILKNFIPNYESSVTQNIFDKGGVMLGK
HHHHCCCCEECCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEEC
TNMDEFAMGSANITSCFGNVISPWKANDDNADLVPGGSSGGSAAAVSGFMASAALGSDTG
CCCHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCC
GSVRQPASFTGLVGFKPTYGRCSRYGMISFASSLDQAGIFTRSVLDSSIMLEAMMGFDEK
CCCCCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCC
DSTSIKAEVPELQSAIGSSMKNMKIGVPLSLGEGSIIEPDIMKMWQDTIELLKNAGAEIV
CCCCEEEHHHHHHHHHHCCHHCEEECCEEECCCCCCCCHHHHHHHHHHHHHHHHCCCEEE
DITLPHAKYGVAVYYVIAPAEASSNLSRYDGVRYGLRVERENMTLDEMYEMTRSTGFGEE
EEECCCCCCCEEEEEEEECCCCCCCHHHHCCCEECEEEECCCCCHHHHHHHHHCCCCCHH
VKRRIMIGTYVLSSSGMDAYYLKAQKVRRLVANDFNNAFAKVDAILLPAAPTAAFKIGEK
HHHHHHHEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCHHEECCCC
QNDPTIMYLNDLFTIPASLAGLPCASVPAGLSARGLPLGIQIIGKQLDEYNVLKVASTIE
CCCCEEEEECHHHHHHHHHCCCCCCCCCCCCCCCCCCEEHHHHHHHCCCCHHHHHHHHHH
SGVKHIKFEPKGF
HHHHEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA