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The map label for this gene is truB [H]

Identifier: 15891994

GI number: 15891994

Start: 63599

End: 64819

Strand: Reverse

Name: truB [H]

Synonym: RC0071

Alternate gene names: 15891994

Gene position: 64819-63599 (Counterclockwise)

Preceding gene: 15891995

Following gene: 15891986

Centisome position: 5.11

GC content: 33.42

Gene sequence:

>1221_bases
ATGTCAATCGAATCCAAAATAAGACAATTAATTGATCAAAACGGTTATATTACTTGCGATGTCCTCATGCAGGAGGTATT
AAACTTAAATCCTACTTCCTATTATAAGCAAGTAAAATCATTAGCTAATGAAGGTGACTTTGTTACGGCTCCTGAAATTT
CACAGCTATTCGGTGAAATTATCGGCTTATGGTGTATCAGAGAGTGGCAAAGAATTGGCTGCCCAAAAAGTCTTAGTTTA
GTAGAGCTTGGTCCTGGTCGAGGTCTATTAATGCGTGATTTACTACGTACTGCAAAATTAGTACCGGAATTTTATAAAGC
CTTATCAATTGAACTAATAGAAATTAATAAAAATTTTATTGCTTATCAAAAGGCTAATTTACAAGATATTAATTTACCGA
TTAGTCATCAATCATTTGTAGAAGATATACCAAAAAAACCTACTATAATAATAGCTAACGAATTCTTTGATGCTATACCG
ATAAAGCAATATATTAAAGTCAAGGAATTATGGTATGAAAGAATATTTGTAGTGCAGCCGGTAGACGAAAGAATTAAATA
CGATAAAATAAGCGTTAATAAGCAACTACAAGAATATCTGCTGTGTACTCATATTGAAGCAAAAGACGGGGCAGTACTTG
AAGAATCTTATAAATCGATAGAAATTATAAAATTTATAGCGCAGCATCTAAAAAGACTAAGCGGCAGTGGCTTAATAATA
GATTACGGCTATGATATAGCTCCGAATGGTAGAACTAGATATCAATATAATCAAACATTACAAGCGGTTAAAAACCATAA
ATATTGCCCCATCCTTGAAAATCTTGGAGAAGCTGATTTATCGGCACATGTGGATTTTTATGCATTGAAGACGGTAGCTA
AAAACAGCAAAATAAACGTAATAGATACAATATCGCAACGAGATTTTTTAATAGAAAATGGAATCTTATTACGTAAACAA
ACATTGCAAGACAAGCTTAATGATAGACATCTTGCCAAATTCGCTTATAGAGAGGAATTTAAAGGAGACACGAAACGCAG
CACCGCAGCGTACACTTTAGTACGTGAGGATGCGAGTATCGGATCGACGTATAAATTACCTCTAGAAGTAGAGTTTGGCA
AGATGTCTGAGCAGGCACAAATAATAGAAAGACAGGTAGAAAGACTAATATCACCGAAACAAATGGGAGAATTATTTAAA
GTATTACAAATTATGAATTAA

Upstream 100 bases:

>100_bases
TATTTAGAGAACCCGACATACAAATCGGGTTTATTTTAGATAGCTTAACTATGGGACAAATTTTATCTGTACCTATGTTA
CTTTTAGGAAGTTATTTAAT

Downstream 100 bases:

>100_bases
TTTAGGTTTTGTAAACATTTCTAAAAAGATTTAATTTTGGCTATTTGTTGCTGCAAATTATAAGATTTTTTTGAAATATG
ACTAACTATTCCGCAAAAAT

Product: hypothetical protein

Products: NA

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase [H]

Number of amino acids: Translated: 406; Mature: 405

Protein sequence:

>406_residues
MSIESKIRQLIDQNGYITCDVLMQEVLNLNPTSYYKQVKSLANEGDFVTAPEISQLFGEIIGLWCIREWQRIGCPKSLSL
VELGPGRGLLMRDLLRTAKLVPEFYKALSIELIEINKNFIAYQKANLQDINLPISHQSFVEDIPKKPTIIIANEFFDAIP
IKQYIKVKELWYERIFVVQPVDERIKYDKISVNKQLQEYLLCTHIEAKDGAVLEESYKSIEIIKFIAQHLKRLSGSGLII
DYGYDIAPNGRTRYQYNQTLQAVKNHKYCPILENLGEADLSAHVDFYALKTVAKNSKINVIDTISQRDFLIENGILLRKQ
TLQDKLNDRHLAKFAYREEFKGDTKRSTAAYTLVREDASIGSTYKLPLEVEFGKMSEQAQIIERQVERLISPKQMGELFK
VLQIMN

Sequences:

>Translated_406_residues
MSIESKIRQLIDQNGYITCDVLMQEVLNLNPTSYYKQVKSLANEGDFVTAPEISQLFGEIIGLWCIREWQRIGCPKSLSL
VELGPGRGLLMRDLLRTAKLVPEFYKALSIELIEINKNFIAYQKANLQDINLPISHQSFVEDIPKKPTIIIANEFFDAIP
IKQYIKVKELWYERIFVVQPVDERIKYDKISVNKQLQEYLLCTHIEAKDGAVLEESYKSIEIIKFIAQHLKRLSGSGLII
DYGYDIAPNGRTRYQYNQTLQAVKNHKYCPILENLGEADLSAHVDFYALKTVAKNSKINVIDTISQRDFLIENGILLRKQ
TLQDKLNDRHLAKFAYREEFKGDTKRSTAAYTLVREDASIGSTYKLPLEVEFGKMSEQAQIIERQVERLISPKQMGELFK
VLQIMN
>Mature_405_residues
SIESKIRQLIDQNGYITCDVLMQEVLNLNPTSYYKQVKSLANEGDFVTAPEISQLFGEIIGLWCIREWQRIGCPKSLSLV
ELGPGRGLLMRDLLRTAKLVPEFYKALSIELIEINKNFIAYQKANLQDINLPISHQSFVEDIPKKPTIIIANEFFDAIPI
KQYIKVKELWYERIFVVQPVDERIKYDKISVNKQLQEYLLCTHIEAKDGAVLEESYKSIEIIKFIAQHLKRLSGSGLIID
YGYDIAPNGRTRYQYNQTLQAVKNHKYCPILENLGEADLSAHVDFYALKTVAKNSKINVIDTISQRDFLIENGILLRKQT
LQDKLNDRHLAKFAYREEFKGDTKRSTAAYTLVREDASIGSTYKLPLEVEFGKMSEQAQIIERQVERLISPKQMGELFKV
LQIMN

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs [H]

COG id: COG1565

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI21396487, Length=341, Percent_Identity=30.4985337243402, Blast_Score=153, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI193211224, Length=346, Percent_Identity=31.7919075144509, Blast_Score=145, Evalue=3e-35,
Organism=Drosophila melanogaster, GI24645885, Length=334, Percent_Identity=29.0419161676647, Blast_Score=148, Evalue=5e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR005728
- InterPro:   IPR014780 [H]

Pfam domain/function: PF01509 TruB_N [H]

EC number: NA

Molecular weight: Translated: 46779; Mature: 46648

Theoretical pI: Translated: 7.87; Mature: 7.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIESKIRQLIDQNGYITCDVLMQEVLNLNPTSYYKQVKSLANEGDFVTAPEISQLFGEI
CCHHHHHHHHHHCCCCEEHHHHHHHHHCCCCHHHHHHHHHHHCCCCEECCCHHHHHHHHH
IGLWCIREWQRIGCPKSLSLVELGPGRGLLMRDLLRTAKLVPEFYKALSIELIEINKNFI
HHHHHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHEEEEEEECCCEE
AYQKANLQDINLPISHQSFVEDIPKKPTIIIANEFFDAIPIKQYIKVKELWYERIFVVQP
EEECCCCEECCCCCCHHHHHHHCCCCCEEEEECCHHHHCCHHHHHHHHHHHHHHEEEECC
VDERIKYDKISVNKQLQEYLLCTHIEAKDGAVLEESYKSIEIIKFIAQHLKRLSGSGLII
HHHHHCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
DYGYDIAPNGRTRYQYNQTLQAVKNHKYCPILENLGEADLSAHVDFYALKTVAKNSKINV
EECCEECCCCCCEEHHHHHHHHHHCCCCCCHHHHCCCCHHHHHHHHHHHHHHHCCCCEEE
IDTISQRDFLIENGILLRKQTLQDKLNDRHLAKFAYREEFKGDTKRSTAAYTLVREDASI
EECCCCCCEEHHCCEEEHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCHHHEEEEECCCCC
GSTYKLPLEVEFGKMSEQAQIIERQVERLISPKQMGELFKVLQIMN
CCEEEEEEEEECCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SIESKIRQLIDQNGYITCDVLMQEVLNLNPTSYYKQVKSLANEGDFVTAPEISQLFGEI
CHHHHHHHHHHCCCCEEHHHHHHHHHCCCCHHHHHHHHHHHCCCCEECCCHHHHHHHHH
IGLWCIREWQRIGCPKSLSLVELGPGRGLLMRDLLRTAKLVPEFYKALSIELIEINKNFI
HHHHHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHEEEEEEECCCEE
AYQKANLQDINLPISHQSFVEDIPKKPTIIIANEFFDAIPIKQYIKVKELWYERIFVVQP
EEECCCCEECCCCCCHHHHHHHCCCCCEEEEECCHHHHCCHHHHHHHHHHHHHHEEEECC
VDERIKYDKISVNKQLQEYLLCTHIEAKDGAVLEESYKSIEIIKFIAQHLKRLSGSGLII
HHHHHCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
DYGYDIAPNGRTRYQYNQTLQAVKNHKYCPILENLGEADLSAHVDFYALKTVAKNSKINV
EECCEECCCCCCEEHHHHHHHHHHCCCCCCHHHHCCCCHHHHHHHHHHHHHHHCCCCEEE
IDTISQRDFLIENGILLRKQTLQDKLNDRHLAKFAYREEFKGDTKRSTAAYTLVREDASI
EECCCCCCEEHHCCEEEHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCHHHEEEEECCCCC
GSTYKLPLEVEFGKMSEQAQIIERQVERLISPKQMGELFKVLQIMN
CCEEEEEEEEECCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA