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The map label for this gene is recF [H]

Identifier: 15891955

GI number: 15891955

Start: 32500

End: 33582

Strand: Reverse

Name: recF [H]

Synonym: RC0032

Alternate gene names: 15891955

Gene position: 33582-32500 (Counterclockwise)

Preceding gene: 15891956

Following gene: 15891954

Centisome position: 2.65

GC content: 31.3

Gene sequence:

>1083_bases
ATGAAAAATATCTTCCTACATTCCTTAAATCTTGAGAATTATCGTAATTTTAAAAATCTTGAACTAAAAACAGATAATAC
ACCAATAATTTTAATAGGTGAAAACGGTAGCGGTAAAACTAATATTTTAGAAGCTATATCGCTATTTTATCCAGGAAGAG
GGCTGAGATCAGCAAAACTTGCCAATGTATGCAAAACTTCAGAAGATCATTGTCTAGTTAAAGCTCTATTGCAAAGTAAG
CTAGGACTTGCAGAATTTACCACCCAATTTAAACGTAGTTCTAATAGAAGGATAACTGAGTATAACGAAAGTAAAATAGC
CAATAATGAGTTAAGTAAATTCACTAGCATGGTATGGCTGACTCCACACATGGAAGGAATCTTTACAAGCGGTAGCAGTG
ATAGAAGAAAATTTCTTGATAGAATAGTTTATAATTTTGATCCAAAGCATGCAGAGCTAGTTAGTAAATACGAATATTAC
ATGCATGAGAGAAATAAAATTCTAGTAGAAGATAGAAGAGATGATAATTGGCTTAAAATTATTGAAGAAAAGATGGCTGA
TATTTCTAACCATATTGCTAATAATCGCTTAAAAACCTTAGAATTTATGCAGCAAGCAATAGATGACCTTGAAAATGAAT
TTCCAAAAGCCGATTTATCGATTGACGGCATTGTTGAACAAAAAATATTAAACGGCAAAAAAAATATTGTTAGTTTTATT
ACTGCAGAACTTTATCAAACAAGAAGCAAAGATAAACTACTTGGTCGCACTAGTTTTGGAGTTCATAAAAGTGACTTTTT
AGTAAAGCATCAAAAGAAAAATATCTTAGCCAAATTCTGTTCTACCGGTGAACAAAAAGCTATATTAATTGCAATAATCC
TTGCTGAAATGAATTATGCTATAAAGCTAACTAAAATAGCACCGATTTTACTTTTAGATGAGGTCTTTGTGCATTTAGAC
GACAAAAGACGTCAATATTTAATCGAATTTTTGACCGGCTTAAATATGCAACTATGGGTTACCACTACCAATCTAGAGGG
AATAGACAATTTTGCAACTAAAGCACAATTGATTAAGTTATAG

Upstream 100 bases:

>100_bases
AAGAACAAACATTAGCATATCAAGGAGCAAAACTACTATGGCAATTTCTAGACGGTATAAATGCAACGTATCAATAATTT
AACTATTATTCTTTCATAAA

Downstream 100 bases:

>100_bases
TTTGTTTAATAATGTCATTCCTGCGAAAGCAGGAATCCAGCATAAAGCGAGATAAATCAAGCTTTTAATTTCAAAAATTT
GCTGTATTTAATACTTTTTT

Product: recombination protein F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 360; Mature: 360

Protein sequence:

>360_residues
MKNIFLHSLNLENYRNFKNLELKTDNTPIILIGENGSGKTNILEAISLFYPGRGLRSAKLANVCKTSEDHCLVKALLQSK
LGLAEFTTQFKRSSNRRITEYNESKIANNELSKFTSMVWLTPHMEGIFTSGSSDRRKFLDRIVYNFDPKHAELVSKYEYY
MHERNKILVEDRRDDNWLKIIEEKMADISNHIANNRLKTLEFMQQAIDDLENEFPKADLSIDGIVEQKILNGKKNIVSFI
TAELYQTRSKDKLLGRTSFGVHKSDFLVKHQKKNILAKFCSTGEQKAILIAIILAEMNYAIKLTKIAPILLLDEVFVHLD
DKRRQYLIEFLTGLNMQLWVTTTNLEGIDNFATKAQLIKL

Sequences:

>Translated_360_residues
MKNIFLHSLNLENYRNFKNLELKTDNTPIILIGENGSGKTNILEAISLFYPGRGLRSAKLANVCKTSEDHCLVKALLQSK
LGLAEFTTQFKRSSNRRITEYNESKIANNELSKFTSMVWLTPHMEGIFTSGSSDRRKFLDRIVYNFDPKHAELVSKYEYY
MHERNKILVEDRRDDNWLKIIEEKMADISNHIANNRLKTLEFMQQAIDDLENEFPKADLSIDGIVEQKILNGKKNIVSFI
TAELYQTRSKDKLLGRTSFGVHKSDFLVKHQKKNILAKFCSTGEQKAILIAIILAEMNYAIKLTKIAPILLLDEVFVHLD
DKRRQYLIEFLTGLNMQLWVTTTNLEGIDNFATKAQLIKL
>Mature_360_residues
MKNIFLHSLNLENYRNFKNLELKTDNTPIILIGENGSGKTNILEAISLFYPGRGLRSAKLANVCKTSEDHCLVKALLQSK
LGLAEFTTQFKRSSNRRITEYNESKIANNELSKFTSMVWLTPHMEGIFTSGSSDRRKFLDRIVYNFDPKHAELVSKYEYY
MHERNKILVEDRRDDNWLKIIEEKMADISNHIANNRLKTLEFMQQAIDDLENEFPKADLSIDGIVEQKILNGKKNIVSFI
TAELYQTRSKDKLLGRTSFGVHKSDFLVKHQKKNILAKFCSTGEQKAILIAIILAEMNYAIKLTKIAPILLLDEVFVHLD
DKRRQYLIEFLTGLNMQLWVTTTNLEGIDNFATKAQLIKL

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP [H]

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family [H]

Homologues:

Organism=Escherichia coli, GI1790135, Length=349, Percent_Identity=23.7822349570201, Blast_Score=84, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001238
- InterPro:   IPR018078
- InterPro:   IPR003395 [H]

Pfam domain/function: PF02463 SMC_N [H]

EC number: NA

Molecular weight: Translated: 41528; Mature: 41528

Theoretical pI: Translated: 9.46; Mature: 9.46

Prosite motif: PS00617 RECF_1 ; PS00618 RECF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNIFLHSLNLENYRNFKNLELKTDNTPIILIGENGSGKTNILEAISLFYPGRGLRSAKL
CCCEEEEECCHHHCCCCCCEEEECCCCCEEEEECCCCCHHHHHHHHHHHCCCCCCCHHHH
ANVCKTSEDHCLVKALLQSKLGLAEFTTQFKRSSNRRITEYNESKIANNELSKFTSMVWL
HHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHEEE
TPHMEGIFTSGSSDRRKFLDRIVYNFDPKHAELVSKYEYYMHERNKILVEDRRDDNWLKI
CCCCCEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHH
IEEKMADISNHIANNRLKTLEFMQQAIDDLENEFPKADLSIDGIVEQKILNGKKNIVSFI
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCHHHHHHHH
TAELYQTRSKDKLLGRTSFGVHKSDFLVKHQKKNILAKFCSTGEQKAILIAIILAEMNYA
HHHHHHHCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHCCCE
IKLTKIAPILLLDEVFVHLDDKRRQYLIEFLTGLNMQLWVTTTNLEGIDNFATKAQLIKL
EEEEHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MKNIFLHSLNLENYRNFKNLELKTDNTPIILIGENGSGKTNILEAISLFYPGRGLRSAKL
CCCEEEEECCHHHCCCCCCEEEECCCCCEEEEECCCCCHHHHHHHHHHHCCCCCCCHHHH
ANVCKTSEDHCLVKALLQSKLGLAEFTTQFKRSSNRRITEYNESKIANNELSKFTSMVWL
HHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHEEE
TPHMEGIFTSGSSDRRKFLDRIVYNFDPKHAELVSKYEYYMHERNKILVEDRRDDNWLKI
CCCCCEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHH
IEEKMADISNHIANNRLKTLEFMQQAIDDLENEFPKADLSIDGIVEQKILNGKKNIVSFI
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCHHHHHHHH
TAELYQTRSKDKLLGRTSFGVHKSDFLVKHQKKNILAKFCSTGEQKAILIAIILAEMNYA
HHHHHHHCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHCCCE
IKLTKIAPILLLDEVFVHLDDKRRQYLIEFLTGLNMQLWVTTTNLEGIDNFATKAQLIKL
EEEEHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA