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The map label for this gene is pcnB [H]

Identifier: 15891938

GI number: 15891938

Start: 13579

End: 14889

Strand: Reverse

Name: pcnB [H]

Synonym: RC0015

Alternate gene names: 15891938

Gene position: 14889-13579 (Counterclockwise)

Preceding gene: 15891939

Following gene: 15891937

Centisome position: 1.17

GC content: 31.05

Gene sequence:

>1311_bases
ATGCAAGTTACACACAAAATACTAAAAATCCCCTCAAAAGGATATAGAAAAATCTTGAGTCTCTTAAATGAAAAAGGACA
AGCAAGACTTATAGGCGGCTGCGTACGTGATGCGTTACTAGATCTCTTGCATAACAAAACTAATAAAGAGGAATTTGAAG
GAGACACGGAACGCAGAATCGCAATGTACATAAACGTACGTGAGGATTCGAGTACCGGATCAACGTACAAATTACCTTTA
GAAGCAAGTTATCCAAGAGATCGACTTGAGAAAGGTAGCTGCGATATAGATATAGCTACTAATTTGACACCTAGTGAAGT
AACAAATATTTTATCTGGAGCTAAAATAAAAACTATTCCGACCGGTTTAAAATTCGGCACGATTACGGCTACTTTAAATA
ATGAAAAATTTGAGATCACGACTCTTAGGAAAGATATTGAATGTAACGGCAGACATGCCAAAGTAATATTCACCAACGAT
TTTGCCGAAGATGCCGCAAGACGAGATTTTACTATTAATGCTTTAAGCTATTGTCCCTTTAAGAACGAGATATATGATTA
TTTTGAAGGGTTTAAGGATTTGCAGCAGGAAAAGGTTGTATTTATAGGGGAAGCTCTAGATAGAATTAAAGAAGATTATT
TGCGAATTCTACGCTTTTTTCGGTTTTCTAGCTATTATGCGAATCAGCTTGATGATGGCAACTTTAAAGCATGTAAAGCT
TTAAAAGACGGCTTAAAAACTTTATCACGAGAGCGAATTAAAAGCGAAATGGATAAAATTATTGTTTCAAAAAGAGCAGC
ACAAATTTTAAAAGCTATGTTTGAAATAGGAATATTAGAACTAATATTCTCTATCCAAAATTATGAAATAAAATTTTTTG
AGCAGGCAAATGATTTTAAATTAGAATTAGTTACTCGATATGCACTCTTATTATATTGTCAAAAAGATTTAAATTTAAAG
GTCTTTCTAGATTGGAAATTCTCAAAACATGAAGCAATGCAAATATTATCAATAACGAACTTTCTGAATGACGCTAAATT
TAACATGAAAAAAATTTGGTTTGAAAAAAAGAATTATAAGGAATATTTATTAGCAGCTAGAATAATCGGTAAATTAAATC
ATTCACAAGTAAAAGAATTTATTCTTAAATATGATGCGTTATTACGTCCTAAATTCCAAGTAAACGGTAATGATTTGTTA
AATATGAATATAGAAAAAAAAAAAATAGGAGCAAAACTAGAATATCTAAAAAATTTTTGGATAGAGCAGGATTTTAAACC
TAGTAAATTAGAGTTGTTGGCGAAATTATGA

Upstream 100 bases:

>100_bases
GGAATGACATAAACGAGCCATATAACAAGGTCCAGTTTTTAGCTAGGATGACATCAAAGTCTTTTTAAAAACTATCCGAT
ACTTTAGGTAAAATCGAATA

Downstream 100 bases:

>100_bases
GAGCTAGTACGTCAAACCGTCATTGCGAGGAAAAATTGAAAATTTTGACGAAGCAATCTCGTGCCAAAATCCTGAGATTT
CCACGCTCCTTTCAGTCGCT

Product: poly(A) polymerase

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT [H]

Number of amino acids: Translated: 436; Mature: 436

Protein sequence:

>436_residues
MQVTHKILKIPSKGYRKILSLLNEKGQARLIGGCVRDALLDLLHNKTNKEEFEGDTERRIAMYINVREDSSTGSTYKLPL
EASYPRDRLEKGSCDIDIATNLTPSEVTNILSGAKIKTIPTGLKFGTITATLNNEKFEITTLRKDIECNGRHAKVIFTND
FAEDAARRDFTINALSYCPFKNEIYDYFEGFKDLQQEKVVFIGEALDRIKEDYLRILRFFRFSSYYANQLDDGNFKACKA
LKDGLKTLSRERIKSEMDKIIVSKRAAQILKAMFEIGILELIFSIQNYEIKFFEQANDFKLELVTRYALLLYCQKDLNLK
VFLDWKFSKHEAMQILSITNFLNDAKFNMKKIWFEKKNYKEYLLAARIIGKLNHSQVKEFILKYDALLRPKFQVNGNDLL
NMNIEKKKIGAKLEYLKNFWIEQDFKPSKLELLAKL

Sequences:

>Translated_436_residues
MQVTHKILKIPSKGYRKILSLLNEKGQARLIGGCVRDALLDLLHNKTNKEEFEGDTERRIAMYINVREDSSTGSTYKLPL
EASYPRDRLEKGSCDIDIATNLTPSEVTNILSGAKIKTIPTGLKFGTITATLNNEKFEITTLRKDIECNGRHAKVIFTND
FAEDAARRDFTINALSYCPFKNEIYDYFEGFKDLQQEKVVFIGEALDRIKEDYLRILRFFRFSSYYANQLDDGNFKACKA
LKDGLKTLSRERIKSEMDKIIVSKRAAQILKAMFEIGILELIFSIQNYEIKFFEQANDFKLELVTRYALLLYCQKDLNLK
VFLDWKFSKHEAMQILSITNFLNDAKFNMKKIWFEKKNYKEYLLAARIIGKLNHSQVKEFILKYDALLRPKFQVNGNDLL
NMNIEKKKIGAKLEYLKNFWIEQDFKPSKLELLAKL
>Mature_436_residues
MQVTHKILKIPSKGYRKILSLLNEKGQARLIGGCVRDALLDLLHNKTNKEEFEGDTERRIAMYINVREDSSTGSTYKLPL
EASYPRDRLEKGSCDIDIATNLTPSEVTNILSGAKIKTIPTGLKFGTITATLNNEKFEITTLRKDIECNGRHAKVIFTND
FAEDAARRDFTINALSYCPFKNEIYDYFEGFKDLQQEKVVFIGEALDRIKEDYLRILRFFRFSSYYANQLDDGNFKACKA
LKDGLKTLSRERIKSEMDKIIVSKRAAQILKAMFEIGILELIFSIQNYEIKFFEQANDFKLELVTRYALLLYCQKDLNLK
VFLDWKFSKHEAMQILSITNFLNDAKFNMKKIWFEKKNYKEYLLAARIIGKLNHSQVKEFILKYDALLRPKFQVNGNDLL
NMNIEKKKIGAKLEYLKNFWIEQDFKPSKLELLAKL

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=362, Percent_Identity=34.8066298342541, Blast_Score=171, Evalue=1e-42,
Organism=Escherichia coli, GI87081691, Length=233, Percent_Identity=25.7510729613734, Blast_Score=76, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI71995920, Length=395, Percent_Identity=30.8860759493671, Blast_Score=156, Evalue=2e-38,
Organism=Drosophila melanogaster, GI281360112, Length=369, Percent_Identity=33.6043360433604, Blast_Score=163, Evalue=3e-40,
Organism=Drosophila melanogaster, GI21357337, Length=369, Percent_Identity=33.6043360433604, Blast_Score=163, Evalue=3e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023068
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 50770; Mature: 50770

Theoretical pI: Translated: 9.63; Mature: 9.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQVTHKILKIPSKGYRKILSLLNEKGQARLIGGCVRDALLDLLHNKTNKEEFEGDTERRI
CCCCHHHHHCCCHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHCCCCCHHHCCCCCCEE
AMYINVREDSSTGSTYKLPLEASYPRDRLEKGSCDIDIATNLTPSEVTNILSGAKIKTIP
EEEEEEECCCCCCCEEEEEECCCCCHHHHCCCCCCEEEECCCCHHHHHHHHCCCEEEECC
TGLKFGTITATLNNEKFEITTLRKDIECNGRHAKVIFTNDFAEDAARRDFTINALSYCPF
CCCEEEEEEEEECCCEEEEEEECCCCCCCCCEEEEEEECCHHHHHHHCCEEEEHEEECCC
KNEIYDYFEGFKDLQQEKVVFIGEALDRIKEDYLRILRFFRFSSYYANQLDDGNFKACKA
HHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
LKDGLKTLSRERIKSEMDKIIVSKRAAQILKAMFEIGILELIFSIQNYEIKFFEQANDFK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCH
LELVTRYALLLYCQKDLNLKVFLDWKFSKHEAMQILSITNFLNDAKFNMKKIWFEKKNYK
HHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCCCHHHHHEECCCHH
EYLLAARIIGKLNHSQVKEFILKYDALLRPKFQVNGNDLLNMNIEKKKIGAKLEYLKNFW
HHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEECCCCEEECCCHHHHHHHHHHHHHHHC
IEQDFKPSKLELLAKL
CCCCCCCHHHHHHHCC
>Mature Secondary Structure
MQVTHKILKIPSKGYRKILSLLNEKGQARLIGGCVRDALLDLLHNKTNKEEFEGDTERRI
CCCCHHHHHCCCHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHCCCCCHHHCCCCCCEE
AMYINVREDSSTGSTYKLPLEASYPRDRLEKGSCDIDIATNLTPSEVTNILSGAKIKTIP
EEEEEEECCCCCCCEEEEEECCCCCHHHHCCCCCCEEEECCCCHHHHHHHHCCCEEEECC
TGLKFGTITATLNNEKFEITTLRKDIECNGRHAKVIFTNDFAEDAARRDFTINALSYCPF
CCCEEEEEEEEECCCEEEEEEECCCCCCCCCEEEEEEECCHHHHHHHCCEEEEHEEECCC
KNEIYDYFEGFKDLQQEKVVFIGEALDRIKEDYLRILRFFRFSSYYANQLDDGNFKACKA
HHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
LKDGLKTLSRERIKSEMDKIIVSKRAAQILKAMFEIGILELIFSIQNYEIKFFEQANDFK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCH
LELVTRYALLLYCQKDLNLKVFLDWKFSKHEAMQILSITNFLNDAKFNMKKIWFEKKNYK
HHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCCCHHHHHEECCCHH
EYLLAARIIGKLNHSQVKEFILKYDALLRPKFQVNGNDLLNMNIEKKKIGAKLEYLKNFW
HHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEECCCCEEECCCHHHHHHHHHHHHHHHC
IEQDFKPSKLELLAKL
CCCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA