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The map label for this gene is gutB [H]

Identifier: 57652508

GI number: 57652508

Start: 271041

End: 272096

Strand: Direct

Name: gutB [H]

Synonym: SACOL0233

Alternate gene names: 57652508

Gene position: 271041-272096 (Clockwise)

Preceding gene: 57652507

Following gene: 57652509

Centisome position: 9.65

GC content: 36.55

Gene sequence:

>1056_bases
GTGAAAGCTTTAGTAAAAACAAGAGAAGGACATGGCAACTTAGAACTTCTTGATAAAGAAGTTGCAACACCGCTAGATGA
TAAAGTAAAGATTAAAGTACATTATGCAGGAATTTGTGGCACAGATATTCATACTTATGAAGGTCATTATAAAGTTAATT
TTCCAGTGACATTAGGTCATGAATTTTCTGGTGAAATCGTTGAAGTTGGAGCAGACGTTAAAGATTTTAAAGTTGGTGAC
CGTGTCACATCTGAAACGACATTCTATGTTTGTAATGAGTGTGAATACTGTAAATCAAAAGACTATAATTTATGCAACCA
TCGAAAAGGTATTGGAACACAAGTTGATGGCGCATTTACTAATTATGTCATTGCACGTGAAGAAAGTTTGCATCATATTC
CAGACGAAGTATCGTATCAGTCTGCAGCTATGACAGAACCATTAGCATGTGCACATCATGGCGTTTCTAAGATTCAAGTC
AATTCAGGCGATGTAGCAGTTGTAATGGGACCTGGGCCAATCGGATTACTTGTAGCACAAGTGTTAAAAAGTAAAGGCGC
AACTGTTGTGGTAACTGGATTGGACAATGACAAAGTCAGATTAGATAAAGCAGAAGCATTGCACATGGATTATGTAGTCA
ATTTACAACAAACAGACTTAAAAACGTATATCAATGGAATTACAGACGGTTACGGTGCAGATGTTGTTGTTGAATGTTCA
GGTGCAGTTCCAGCAGCACGACAAGGTTTGGATATTTTACGCAAAAAAGGTTTCTACAGTCAAATAGGTATTTTTAAGGA
TGCTGAAATTCCATTTGATATGGAAAAAGTGATTCAAAAAGAAATAACAGTTGTTGGTAGTAGAAGTCAAAAGCCAGCAG
ATTGGGAACCTTCATTGCAACTTATGGCGGATGGTTTAGTAAATGCTGAAGCTTTGGTGACAAAAATATATGATATTTCG
AAATGGGACGAGGCGTATCAACATTTAAAATCCGGCGAAGGTATTAAAGTATTACTTAAGCCGCTCGATTTAGATGAAAA
TGAAGGAGAGAATTAA

Upstream 100 bases:

>100_bases
GTTCATCTGAAATTTCAAGTATTGATCAAGGTGGTAATATCGTTAACTATCTTATCTTTAAACTATTTAGTCTATTCAAT
TAAAAATCGAGGTGTTTGTG

Downstream 100 bases:

>100_bases
TATGGTAGAATCAATGCTAACTTTTATGCTTGGGCCATTAAGACAAATCACTGATTTTTATATGGAACATTTACTCGTAA
GTAATTCCATTGTCATTGCA

Product: sorbitol dehydrogenase

Products: NA

Alternate protein names: Glucitol dehydrogenase; L-iditol 2-dehydrogenase [H]

Number of amino acids: Translated: 351; Mature: 351

Protein sequence:

>351_residues
MKALVKTREGHGNLELLDKEVATPLDDKVKIKVHYAGICGTDIHTYEGHYKVNFPVTLGHEFSGEIVEVGADVKDFKVGD
RVTSETTFYVCNECEYCKSKDYNLCNHRKGIGTQVDGAFTNYVIAREESLHHIPDEVSYQSAAMTEPLACAHHGVSKIQV
NSGDVAVVMGPGPIGLLVAQVLKSKGATVVVTGLDNDKVRLDKAEALHMDYVVNLQQTDLKTYINGITDGYGADVVVECS
GAVPAARQGLDILRKKGFYSQIGIFKDAEIPFDMEKVIQKEITVVGSRSQKPADWEPSLQLMADGLVNAEALVTKIYDIS
KWDEAYQHLKSGEGIKVLLKPLDLDENEGEN

Sequences:

>Translated_351_residues
MKALVKTREGHGNLELLDKEVATPLDDKVKIKVHYAGICGTDIHTYEGHYKVNFPVTLGHEFSGEIVEVGADVKDFKVGD
RVTSETTFYVCNECEYCKSKDYNLCNHRKGIGTQVDGAFTNYVIAREESLHHIPDEVSYQSAAMTEPLACAHHGVSKIQV
NSGDVAVVMGPGPIGLLVAQVLKSKGATVVVTGLDNDKVRLDKAEALHMDYVVNLQQTDLKTYINGITDGYGADVVVECS
GAVPAARQGLDILRKKGFYSQIGIFKDAEIPFDMEKVIQKEITVVGSRSQKPADWEPSLQLMADGLVNAEALVTKIYDIS
KWDEAYQHLKSGEGIKVLLKPLDLDENEGEN
>Mature_351_residues
MKALVKTREGHGNLELLDKEVATPLDDKVKIKVHYAGICGTDIHTYEGHYKVNFPVTLGHEFSGEIVEVGADVKDFKVGD
RVTSETTFYVCNECEYCKSKDYNLCNHRKGIGTQVDGAFTNYVIAREESLHHIPDEVSYQSAAMTEPLACAHHGVSKIQV
NSGDVAVVMGPGPIGLLVAQVLKSKGATVVVTGLDNDKVRLDKAEALHMDYVVNLQQTDLKTYINGITDGYGADVVVECS
GAVPAARQGLDILRKKGFYSQIGIFKDAEIPFDMEKVIQKEITVVGSRSQKPADWEPSLQLMADGLVNAEALVTKIYDIS
KWDEAYQHLKSGEGIKVLLKPLDLDENEGEN

Specific function: Reduces glucitol to fructose [H]

COG id: COG1063

COG function: function code ER; Threonine dehydrogenase and related Zn-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI156627571, Length=346, Percent_Identity=29.7687861271676, Blast_Score=147, Evalue=1e-35,
Organism=Homo sapiens, GI4501933, Length=360, Percent_Identity=26.3888888888889, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI4501929, Length=356, Percent_Identity=26.685393258427, Blast_Score=90, Evalue=3e-18,
Organism=Homo sapiens, GI71743840, Length=213, Percent_Identity=31.4553990610329, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI262073058, Length=213, Percent_Identity=31.4553990610329, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI156523966, Length=359, Percent_Identity=26.4623955431755, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI34577061, Length=361, Percent_Identity=26.8698060941828, Blast_Score=86, Evalue=6e-17,
Organism=Homo sapiens, GI71565154, Length=365, Percent_Identity=25.7534246575342, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI4501939, Length=353, Percent_Identity=26.3456090651558, Blast_Score=83, Evalue=3e-16,
Organism=Escherichia coli, GI1788075, Length=365, Percent_Identity=30.1369863013699, Blast_Score=161, Evalue=5e-41,
Organism=Escherichia coli, GI1790045, Length=348, Percent_Identity=31.6091954022989, Blast_Score=155, Evalue=3e-39,
Organism=Escherichia coli, GI1788073, Length=325, Percent_Identity=30.7692307692308, Blast_Score=149, Evalue=3e-37,
Organism=Escherichia coli, GI226510992, Length=312, Percent_Identity=31.4102564102564, Blast_Score=148, Evalue=5e-37,
Organism=Escherichia coli, GI1787863, Length=336, Percent_Identity=30.952380952381, Blast_Score=131, Evalue=5e-32,
Organism=Escherichia coli, GI1790718, Length=306, Percent_Identity=27.4509803921569, Blast_Score=114, Evalue=7e-27,
Organism=Escherichia coli, GI1788407, Length=326, Percent_Identity=25.7668711656442, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI87082125, Length=346, Percent_Identity=27.1676300578035, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI1786552, Length=366, Percent_Identity=28.9617486338798, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI87081918, Length=322, Percent_Identity=26.3975155279503, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1786825, Length=391, Percent_Identity=24.5524296675192, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1787572, Length=285, Percent_Identity=27.3684210526316, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI87082401, Length=349, Percent_Identity=21.2034383954155, Blast_Score=63, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17562876, Length=343, Percent_Identity=31.1953352769679, Blast_Score=157, Evalue=9e-39,
Organism=Caenorhabditis elegans, GI17562878, Length=341, Percent_Identity=30.2052785923754, Blast_Score=144, Evalue=5e-35,
Organism=Caenorhabditis elegans, GI71988145, Length=339, Percent_Identity=28.6135693215339, Blast_Score=128, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI17562584, Length=338, Percent_Identity=28.9940828402367, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17562582, Length=333, Percent_Identity=25.8258258258258, Blast_Score=113, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI25146526, Length=361, Percent_Identity=26.5927977839335, Blast_Score=99, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI71997431, Length=363, Percent_Identity=26.4462809917355, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17565904, Length=348, Percent_Identity=26.1494252873563, Blast_Score=90, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6319955, Length=344, Percent_Identity=31.9767441860465, Blast_Score=144, Evalue=3e-35,
Organism=Saccharomyces cerevisiae, GI6322619, Length=344, Percent_Identity=31.9767441860465, Blast_Score=143, Evalue=4e-35,
Organism=Saccharomyces cerevisiae, GI6323099, Length=310, Percent_Identity=32.5806451612903, Blast_Score=142, Evalue=8e-35,
Organism=Saccharomyces cerevisiae, GI6319258, Length=388, Percent_Identity=29.1237113402062, Blast_Score=129, Evalue=5e-31,
Organism=Saccharomyces cerevisiae, GI6319257, Length=390, Percent_Identity=27.6923076923077, Blast_Score=119, Evalue=6e-28,
Organism=Saccharomyces cerevisiae, GI6324486, Length=333, Percent_Identity=27.9279279279279, Blast_Score=115, Evalue=9e-27,
Organism=Saccharomyces cerevisiae, GI6319621, Length=333, Percent_Identity=27.027027027027, Blast_Score=113, Evalue=5e-26,
Organism=Saccharomyces cerevisiae, GI6323961, Length=333, Percent_Identity=27.027027027027, Blast_Score=112, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6323729, Length=333, Percent_Identity=25.2252252252252, Blast_Score=91, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6320033, Length=367, Percent_Identity=25.6130790190736, Blast_Score=80, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6323980, Length=240, Percent_Identity=26.6666666666667, Blast_Score=71, Evalue=3e-13,
Organism=Drosophila melanogaster, GI17137530, Length=350, Percent_Identity=29.4285714285714, Blast_Score=131, Evalue=6e-31,
Organism=Drosophila melanogaster, GI17737897, Length=355, Percent_Identity=29.5774647887324, Blast_Score=131, Evalue=6e-31,
Organism=Drosophila melanogaster, GI17737895, Length=366, Percent_Identity=27.8688524590164, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI221457811, Length=313, Percent_Identity=24.9201277955272, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI45550770, Length=313, Percent_Identity=24.9201277955272, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI45551930, Length=313, Percent_Identity=24.9201277955272, Blast_Score=89, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR002328
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: =1.1.1.14 [H]

Molecular weight: Translated: 38485; Mature: 38485

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS00059 ADH_ZINC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKALVKTREGHGNLELLDKEVATPLDDKVKIKVHYAGICGTDIHTYEGHYKVNFPVTLGH
CCCCEECCCCCCCEEEECHHHCCCCCCCEEEEEEEEEEECCCCEEECCEEEEEEEEEECC
EFSGEIVEVGADVKDFKVGDRVTSETTFYVCNECEYCKSKDYNLCNHRKGIGTQVDGAFT
CCCCCEEEECCCCCEEECCCCCCCCEEEEEECCCCHHCCCCCCCHHCCCCCCCCCCCCHH
NYVIAREESLHHIPDEVSYQSAAMTEPLACAHHGVSKIQVNSGDVAVVMGPGPIGLLVAQ
EEEEEECCCHHCCCCHHCCCHHHCCCCHHHHHCCCEEEEECCCCEEEEECCCCHHHHHHH
VLKSKGATVVVTGLDNDKVRLDKAEALHMDYVVNLQQTDLKTYINGITDGYGADVVVECS
HHHCCCCEEEEECCCCCCEEECHHHEEEEEEEEEEHHHHHHHHHHHCCCCCCCCEEEEEC
GAVPAARQGLDILRKKGFYSQIGIFKDAEIPFDMEKVIQKEITVVGSRSQKPADWEPSLQ
CCCCHHHHHHHHHHHCCCHHHHCEEECCCCCCCHHHHHHHHHEEEECCCCCCCCCCCCHH
LMADGLVNAEALVTKIYDISKWDEAYQHLKSGEGIKVLLKPLDLDENEGEN
HHHCCCCCHHHHHHHHHCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCC
>Mature Secondary Structure
MKALVKTREGHGNLELLDKEVATPLDDKVKIKVHYAGICGTDIHTYEGHYKVNFPVTLGH
CCCCEECCCCCCCEEEECHHHCCCCCCCEEEEEEEEEEECCCCEEECCEEEEEEEEEECC
EFSGEIVEVGADVKDFKVGDRVTSETTFYVCNECEYCKSKDYNLCNHRKGIGTQVDGAFT
CCCCCEEEECCCCCEEECCCCCCCCEEEEEECCCCHHCCCCCCCHHCCCCCCCCCCCCHH
NYVIAREESLHHIPDEVSYQSAAMTEPLACAHHGVSKIQVNSGDVAVVMGPGPIGLLVAQ
EEEEEECCCHHCCCCHHCCCHHHCCCCHHHHHCCCEEEEECCCCEEEEECCCCHHHHHHH
VLKSKGATVVVTGLDNDKVRLDKAEALHMDYVVNLQQTDLKTYINGITDGYGADVVVECS
HHHCCCCEEEEECCCCCCEEECHHHEEEEEEEEEEHHHHHHHHHHHCCCCCCCCEEEEEC
GAVPAARQGLDILRKKGFYSQIGIFKDAEIPFDMEKVIQKEITVVGSRSQKPADWEPSLQ
CCCCHHHHHHHHHHHCCCHHHHCEEECCCCCCCHHHHHHHHHEEEECCCCCCCCCCCCHH
LMADGLVNAEALVTKIYDISKWDEAYQHLKSGEGIKVLLKPLDLDENEGEN
HHHCCCCCHHHHHHHHHCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10086842; 11058132 [H]