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The map label for this gene is yrkE [H]

Identifier: 57652416

GI number: 57652416

Start: 54775

End: 55842

Strand: Reverse

Name: yrkE [H]

Synonym: SACOL0047

Alternate gene names: 57652416

Gene position: 55842-54775 (Counterclockwise)

Preceding gene: 57652421

Following gene: 57652415

Centisome position: 1.99

GC content: 31.74

Gene sequence:

>1068_bases
ATGTCACAATATAAAACGAAACACATTAATGATTTCACTAAAAAAGAATTACAAGAATTGAGTTCTAAGGGTCAATTAAT
TGACGTTCGACATCCTGAAGAATTTGAATTAGGACACATTAAAAACGCTTTATTACATTCAGTTGAAAATATAGAGACTT
TTAAACAAAGCAAAAATAAAACTTACTATATCTATTGTAAAAGTGGTAATAGAAGTAGAAAAGCCAGTCAATTTCTTGCA
CAACGCGGATACGATGTAGTGAATTTAGACGGAGGTTATACCGCATACGAACAACAACATCATAATGATAATTTATCTAA
AGTAAAAGAAGATAGAGAAATTAAAGATAATCGTAAGACATTCAATTATAGTAATCTACAGTGTCCAGGACCTATTGTTA
ATATTAGTAAAGAAATCAAAAACATCGCTATTGGCGATCAAATAGAAGTTGTTGTGACTGATCACGGATTTTTAAATGAT
ATTAAAAGTTGGGTCAAACAAACTGGACATACGCTTGTTAGACTCAACGATTTTGGCAATGAAATTCGCGCTATTATTCA
AAAAGAAGAAAATAAAAATATAGAAGTGACACACACGAAAAGTGGAACAACAATTGTTTTATTTAGCGGCGAGCTAGATA
AAGCAGTTGCAGCAATGATTATTGCTAATGGTGCTAAAGCTGCAGGTAGAGATGTAACCATTTTTTGCACATTTTGGGGC
TTAAATGCACTTAAAAAAATTCAAAGTCAACGTATTAAGAAAAAAGGTATTGCAAAACTTTTTGACTTCATGCTTCCAAA
TTCACCAATTAACATGCCTATTTCTAAAATGAATATGTTTGGTTTAGGCAATCTAATGATGCGTTATGTCATGAAAAAGA
AAAATGTCGATACTTTACCTTCTCTTATTGATCAAGCAGTTGAGCAAGGTGTTAAACTTATCGCTTGTACAATGAGTATG
GATGTGATGGGCATTACAAAAGAAGAACTACGTGATGATGTAGAATACGGTGGCGTAGGTGCATATATTGGATATACAGA
ACAAGCTAATCACAATTTATTTATATAA

Upstream 100 bases:

>100_bases
TATTGATTAAATTTTAGTTTGTCAAATACCCCCATAGGTATTTGACAAACTATCTAATTCGTTATAATATACCCCCATAG
GTATTTAAAAGGAGGCAATT

Downstream 100 bases:

>100_bases
TTAGAAAGGAATGATATTATGTTTTTTAAACAATTTTATGATAAACATTTATCTCAAGCATCTTATTTAATCGGTTGTCA
AAAAACTGGAGAAGCCATGA

Product: hypothetical protein

Products: H+; Sulfite; Thiocyanate [C]

Alternate protein names: NA

Number of amino acids: Translated: 355; Mature: 354

Protein sequence:

>355_residues
MSQYKTKHINDFTKKELQELSSKGQLIDVRHPEEFELGHIKNALLHSVENIETFKQSKNKTYYIYCKSGNRSRKASQFLA
QRGYDVVNLDGGYTAYEQQHHNDNLSKVKEDREIKDNRKTFNYSNLQCPGPIVNISKEIKNIAIGDQIEVVVTDHGFLND
IKSWVKQTGHTLVRLNDFGNEIRAIIQKEENKNIEVTHTKSGTTIVLFSGELDKAVAAMIIANGAKAAGRDVTIFCTFWG
LNALKKIQSQRIKKKGIAKLFDFMLPNSPINMPISKMNMFGLGNLMMRYVMKKKNVDTLPSLIDQAVEQGVKLIACTMSM
DVMGITKEELRDDVEYGGVGAYIGYTEQANHNLFI

Sequences:

>Translated_355_residues
MSQYKTKHINDFTKKELQELSSKGQLIDVRHPEEFELGHIKNALLHSVENIETFKQSKNKTYYIYCKSGNRSRKASQFLA
QRGYDVVNLDGGYTAYEQQHHNDNLSKVKEDREIKDNRKTFNYSNLQCPGPIVNISKEIKNIAIGDQIEVVVTDHGFLND
IKSWVKQTGHTLVRLNDFGNEIRAIIQKEENKNIEVTHTKSGTTIVLFSGELDKAVAAMIIANGAKAAGRDVTIFCTFWG
LNALKKIQSQRIKKKGIAKLFDFMLPNSPINMPISKMNMFGLGNLMMRYVMKKKNVDTLPSLIDQAVEQGVKLIACTMSM
DVMGITKEELRDDVEYGGVGAYIGYTEQANHNLFI
>Mature_354_residues
SQYKTKHINDFTKKELQELSSKGQLIDVRHPEEFELGHIKNALLHSVENIETFKQSKNKTYYIYCKSGNRSRKASQFLAQ
RGYDVVNLDGGYTAYEQQHHNDNLSKVKEDREIKDNRKTFNYSNLQCPGPIVNISKEIKNIAIGDQIEVVVTDHGFLNDI
KSWVKQTGHTLVRLNDFGNEIRAIIQKEENKNIEVTHTKSGTTIVLFSGELDKAVAAMIIANGAKAAGRDVTIFCTFWGL
NALKKIQSQRIKKKGIAKLFDFMLPNSPINMPISKMNMFGLGNLMMRYVMKKKNVDTLPSLIDQAVEQGVKLIACTMSMD
VMGITKEELRDDVEYGGVGAYIGYTEQANHNLFI

Specific function: Catalyzes, Although With Low Efficiency, The Sulfur Transfer Reaction From Thiosulfate To Cyanide. The Relatively Low Affinity Of Glpe For Both Thiosulfate And Cyanide Suggests That These Compounds Are Not The Physiological Substrates. Thioredoxin 1 Or Re

COG id: COG2210

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003787 [H]

Pfam domain/function: PF02635 DrsE [H]

EC number: 2.8.1.1 [C]

Molecular weight: Translated: 40206; Mature: 40075

Theoretical pI: Translated: 9.30; Mature: 9.30

Prosite motif: PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQYKTKHINDFTKKELQELSSKGQLIDVRHPEEFELGHIKNALLHSVENIETFKQSKNK
CCCCCCCCCCHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
TYYIYCKSGNRSRKASQFLAQRGYDVVNLDGGYTAYEQQHHNDNLSKVKEDREIKDNRKT
EEEEEECCCCCCHHHHHHHHHCCCCEEECCCCCCHHHHHHCCCCHHHHHHHHHHHCCCCC
FNYSNLQCPGPIVNISKEIKNIAIGDQIEVVVTDHGFLNDIKSWVKQTGHTLVRLNDFGN
CCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHCCCEEEEEHHHHH
EIRAIIQKEENKNIEVTHTKSGTTIVLFSGELDKAVAAMIIANGAKAAGRDVTIFCTFWG
HHHHHHHHCCCCCEEEEEECCCCEEEEECCCHHHHHHHHHHCCCCCCCCCCEEEEEEECC
LNALKKIQSQRIKKKGIAKLFDFMLPNSPINMPISKMNMFGLGNLMMRYVMKKKNVDTLP
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHH
SLIDQAVEQGVKLIACTMSMDVMGITKEELRDDVEYGGVGAYIGYTEQANHNLFI
HHHHHHHHCCCEEEEEEECCCEECCCHHHHHHHHHHCCEEEEEECCCCCCCCCCC
>Mature Secondary Structure 
SQYKTKHINDFTKKELQELSSKGQLIDVRHPEEFELGHIKNALLHSVENIETFKQSKNK
CCCCCCCCCHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
TYYIYCKSGNRSRKASQFLAQRGYDVVNLDGGYTAYEQQHHNDNLSKVKEDREIKDNRKT
EEEEEECCCCCCHHHHHHHHHCCCCEEECCCCCCHHHHHHCCCCHHHHHHHHHHHCCCCC
FNYSNLQCPGPIVNISKEIKNIAIGDQIEVVVTDHGFLNDIKSWVKQTGHTLVRLNDFGN
CCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHCCCEEEEEHHHHH
EIRAIIQKEENKNIEVTHTKSGTTIVLFSGELDKAVAAMIIANGAKAAGRDVTIFCTFWG
HHHHHHHHCCCCCEEEEEECCCCEEEEECCCHHHHHHHHHHCCCCCCCCCCEEEEEEECC
LNALKKIQSQRIKKKGIAKLFDFMLPNSPINMPISKMNMFGLGNLMMRYVMKKKNVDTLP
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHH
SLIDQAVEQGVKLIACTMSMDVMGITKEELRDDVEYGGVGAYIGYTEQANHNLFI
HHHHHHHHCCCEEEEEEECCCEECCCHHHHHHHHHHCCEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Cyanide; Thiosulfate [C]

Specific reaction: Cyanide + Thiosulfate --> H+ + Sulfite + Thiocyanate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377 [H]