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The map label for this gene is pdhC

Identifier: 57651704

GI number: 57651704

Start: 1114254

End: 1115546

Strand: Direct

Name: pdhC

Synonym: SACOL1104

Alternate gene names: 57651704

Gene position: 1114254-1115546 (Clockwise)

Preceding gene: 57651703

Following gene: 57651705

Centisome position: 39.66

GC content: 37.9

Gene sequence:

>1293_bases
GTGGCATTTGAATTTAGATTACCCGATATCGGGGAAGGTATCCACGAAGGTGAAATTGTAAAATGGTTTGTTAAAGCTGG
AGATACTATTGAAGAAGACGATGTTTTAGCTGAGGTACAAAACGATAAATCAGTAGTAGAAATCCCATCACCAGTATCTG
GTACTGTAGAAGAAGTTATGGTAGAAGAAGGTACAGTAGCTGTAGTTGGTGACGTTATTGTTAAAATCGATGCACCTGAT
GCAGAAGATATGCAATTTAAAGGTCATGATGATGATTCATCATCTAAAGAAGAACCTGCGAAAGAGGAAGCGCCAGCAGA
GCAAGCACCTGTAGCTACTCAAACTGAAGAAGTAGATGAAAACAGAACTGTTAAAGCAATGCCTTCAGTACGTAAATACG
CACGTGAAAAAGGTGTTAACATTAAAGCAGTTTCTGGATCTGGTAAAAATGGTCGTATTACAAAAGAAGATGTAGATGCA
TACTTAAATGGTGGTGCACCAACAGCTTCAAATGAATCAGCTGCTTCAGCTACAAGTGAAGAAGTTGCTGAAACTCCTGC
AGCACCTGCAGCAGTAACATTAGAAGGCGACTTCCCAGAAACAACTGAAAAAATCCCTGCTATGCGTAGAGCAATTGCGA
AAGCAATGGTTAACTCTAAGCATACTGCACCTCATGTAACATTAATGGATGAAATTGATGTTCAAGCATTATGGGATCAC
CGTAAGAAATTTAAAGAAATCGCAGCTGAACAAGGTACTAAGTTAACATTCTTACCTTATGTTGTTAAAGCACTTGTTTC
TGCATTGAAAAAATACCCAGCACTTAACACTTCATTCAATGAAGAAGCTGGTGAAATCGTTCATAAACATTACTGGAATA
TCGGTATTGCAGCAGACACTGATAGAGGATTATTAGTACCTGTTGTTAAACATGCTGATCGTAAGTCTATTTTCCAAATT
TCAGATGAAATTAATGAATTAGCTGTTAAAGCACGTGATGGTAAATTAACAGCCGATGAAATGAAAGGTGCTACATGCAC
AATCAGTAATATCGGTTCAGCTGGTGGACAATGGTTCACTCCAGTTATCAATCACCCAGAAGTAGCAATCTTAGGAATTG
GCCGTATTGCTCAAAAACCTATCGTTAAAGATGGAGAAATTGTTGCAGCACCAGTATTAGCATTATCATTAAGCTTTGAC
CACAGACAAATTGATGGTGCAACTGGCCAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATT
AATGGAGGGGTAA

Upstream 100 bases:

>100_bases
AGAATTTTAATACATTTTAAAAGTTAACGAAGTTAGCGTATTTTAGTCTCATTGATTAAAATGAAATGTTTAATTTACGA
AATCTTAGGAGGGCAAAAAC

Downstream 100 bases:

>100_bases
AACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACTATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAAT
TCGTGCAGCTCAATTAGGAC

Product: branched-chain alpha-keto acid dehydrogenase subunit E2

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2

Number of amino acids: Translated: 430; Mature: 429

Protein sequence:

>430_residues
MAFEFRLPDIGEGIHEGEIVKWFVKAGDTIEEDDVLAEVQNDKSVVEIPSPVSGTVEEVMVEEGTVAVVGDVIVKIDAPD
AEDMQFKGHDDDSSSKEEPAKEEAPAEQAPVATQTEEVDENRTVKAMPSVRKYAREKGVNIKAVSGSGKNGRITKEDVDA
YLNGGAPTASNESAASATSEEVAETPAAPAAVTLEGDFPETTEKIPAMRRAIAKAMVNSKHTAPHVTLMDEIDVQALWDH
RKKFKEIAAEQGTKLTFLPYVVKALVSALKKYPALNTSFNEEAGEIVHKHYWNIGIAADTDRGLLVPVVKHADRKSIFQI
SDEINELAVKARDGKLTADEMKGATCTISNIGSAGGQWFTPVINHPEVAILGIGRIAQKPIVKDGEIVAAPVLALSLSFD
HRQIDGATGQNAMNHIKRLLNNPELLLMEG

Sequences:

>Translated_430_residues
MAFEFRLPDIGEGIHEGEIVKWFVKAGDTIEEDDVLAEVQNDKSVVEIPSPVSGTVEEVMVEEGTVAVVGDVIVKIDAPD
AEDMQFKGHDDDSSSKEEPAKEEAPAEQAPVATQTEEVDENRTVKAMPSVRKYAREKGVNIKAVSGSGKNGRITKEDVDA
YLNGGAPTASNESAASATSEEVAETPAAPAAVTLEGDFPETTEKIPAMRRAIAKAMVNSKHTAPHVTLMDEIDVQALWDH
RKKFKEIAAEQGTKLTFLPYVVKALVSALKKYPALNTSFNEEAGEIVHKHYWNIGIAADTDRGLLVPVVKHADRKSIFQI
SDEINELAVKARDGKLTADEMKGATCTISNIGSAGGQWFTPVINHPEVAILGIGRIAQKPIVKDGEIVAAPVLALSLSFD
HRQIDGATGQNAMNHIKRLLNNPELLLMEG
>Mature_429_residues
AFEFRLPDIGEGIHEGEIVKWFVKAGDTIEEDDVLAEVQNDKSVVEIPSPVSGTVEEVMVEEGTVAVVGDVIVKIDAPDA
EDMQFKGHDDDSSSKEEPAKEEAPAEQAPVATQTEEVDENRTVKAMPSVRKYAREKGVNIKAVSGSGKNGRITKEDVDAY
LNGGAPTASNESAASATSEEVAETPAAPAAVTLEGDFPETTEKIPAMRRAIAKAMVNSKHTAPHVTLMDEIDVQALWDHR
KKFKEIAAEQGTKLTFLPYVVKALVSALKKYPALNTSFNEEAGEIVHKHYWNIGIAADTDRGLLVPVVKHADRKSIFQIS
DEINELAVKARDGKLTADEMKGATCTISNIGSAGGQWFTPVINHPEVAILGIGRIAQKPIVKDGEIVAAPVLALSLSFDH
RQIDGATGQNAMNHIKRLLNNPELLLMEG

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain

Homologues:

Organism=Homo sapiens, GI110671329, Length=431, Percent_Identity=36.1948955916473, Blast_Score=236, Evalue=4e-62,
Organism=Homo sapiens, GI31711992, Length=445, Percent_Identity=28.9887640449438, Blast_Score=170, Evalue=2e-42,
Organism=Homo sapiens, GI203098753, Length=452, Percent_Identity=27.212389380531, Blast_Score=149, Evalue=5e-36,
Organism=Homo sapiens, GI203098816, Length=452, Percent_Identity=27.212389380531, Blast_Score=149, Evalue=7e-36,
Organism=Homo sapiens, GI19923748, Length=255, Percent_Identity=34.1176470588235, Blast_Score=147, Evalue=1e-35,
Organism=Homo sapiens, GI260898739, Length=167, Percent_Identity=31.7365269461078, Blast_Score=91, Evalue=2e-18,
Organism=Escherichia coli, GI1786946, Length=429, Percent_Identity=30.5361305361305, Blast_Score=224, Evalue=1e-59,
Organism=Escherichia coli, GI1786305, Length=432, Percent_Identity=35.1851851851852, Blast_Score=221, Evalue=8e-59,
Organism=Caenorhabditis elegans, GI17537937, Length=433, Percent_Identity=33.7182448036951, Blast_Score=232, Evalue=2e-61,
Organism=Caenorhabditis elegans, GI25146366, Length=430, Percent_Identity=30.6976744186047, Blast_Score=191, Evalue=9e-49,
Organism=Caenorhabditis elegans, GI17560088, Length=449, Percent_Identity=30.5122494432071, Blast_Score=177, Evalue=6e-45,
Organism=Caenorhabditis elegans, GI17538894, Length=223, Percent_Identity=30.0448430493274, Blast_Score=107, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6320352, Length=425, Percent_Identity=31.5294117647059, Blast_Score=202, Evalue=6e-53,
Organism=Saccharomyces cerevisiae, GI6324258, Length=450, Percent_Identity=30, Blast_Score=166, Evalue=8e-42,
Organism=Drosophila melanogaster, GI18859875, Length=427, Percent_Identity=34.4262295081967, Blast_Score=234, Evalue=1e-61,
Organism=Drosophila melanogaster, GI24645909, Length=245, Percent_Identity=32.2448979591837, Blast_Score=131, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24582497, Length=233, Percent_Identity=32.618025751073, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI20129315, Length=233, Percent_Identity=32.618025751073, Blast_Score=126, Evalue=2e-29,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): ODP2_STAAC (Q5HGY9)

Other databases:

- EMBL:   CP000046
- RefSeq:   YP_185968.1
- ProteinModelPortal:   Q5HGY9
- STRING:   Q5HGY9
- EnsemblBacteria:   EBSTAT00000009842
- GeneID:   3237847
- GenomeReviews:   CP000046_GR
- KEGG:   sac:SACOL1104
- TIGR:   SACOL1104
- eggNOG:   COG0508
- GeneTree:   EBGT00050000024152
- HOGENOM:   HBG630916
- OMA:   TSIDDKT
- ProtClustDB:   PRK11856
- BioCyc:   SAUR93062:SACOL1104-MONOMER
- GO:   GO:0006096
- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- Gene3D:   G3DSA:4.10.320.10
- Gene3D:   G3DSA:3.30.559.10

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding; SSF47005 E3_bd; SSF51230 Hybrid_motif

EC number: =2.3.1.12

Molecular weight: Translated: 46383; Mature: 46252

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS50968 BIOTINYL_LIPOYL; PS00189 LIPOYL

Important sites: ACT_SITE 401-401

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFEFRLPDIGEGIHEGEIVKWFVKAGDTIEEDDVLAEVQNDKSVVEIPSPVSGTVEEVM
CCEEECCCHHCCCCCCCHHEEEEECCCCCCCCCHHHHHHCCCCCEEECCCCCCCCHHHHH
VEEGTVAVVGDVIVKIDAPDAEDMQFKGHDDDSSSKEEPAKEEAPAEQAPVATQTEEVDE
HCCCCEEEEEEEEEEECCCCCCCCEECCCCCCCCCCCCCCHHHCCCHHCCCCCCHHHHCC
NRTVKAMPSVRKYAREKGVNIKAVSGSGKNGRITKEDVDAYLNGGAPTASNESAASATSE
CCCEEHHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHCCCCCCCCCCCCHHHCCHH
EVAETPAAPAAVTLEGDFPETTEKIPAMRRAIAKAMVNSKHTAPHVTLMDEIDVQALWDH
HHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHH
RKKFKEIAAEQGTKLTFLPYVVKALVSALKKYPALNTSFNEEAGEIVHKHYWNIGIAADT
HHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCEEEEEECC
DRGLLVPVVKHADRKSIFQISDEINELAVKARDGKLTADEMKGATCTISNIGSAGGQWFT
CCCEEEHHHHCCCHHHHHHHHHHHHHHEEEECCCCEEHHHHCCCEEEECCCCCCCCCEEC
PVINHPEVAILGIGRIAQKPIVKDGEIVAAPVLALSLSFDHRQIDGATGQNAMNHIKRLL
CCCCCCCEEEEECCHHHCCCCCCCCCEEEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHH
NNPELLLMEG
CCCCEEEECC
>Mature Secondary Structure 
AFEFRLPDIGEGIHEGEIVKWFVKAGDTIEEDDVLAEVQNDKSVVEIPSPVSGTVEEVM
CEEECCCHHCCCCCCCHHEEEEECCCCCCCCCHHHHHHCCCCCEEECCCCCCCCHHHHH
VEEGTVAVVGDVIVKIDAPDAEDMQFKGHDDDSSSKEEPAKEEAPAEQAPVATQTEEVDE
HCCCCEEEEEEEEEEECCCCCCCCEECCCCCCCCCCCCCCHHHCCCHHCCCCCCHHHHCC
NRTVKAMPSVRKYAREKGVNIKAVSGSGKNGRITKEDVDAYLNGGAPTASNESAASATSE
CCCEEHHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHCCCCCCCCCCCCHHHCCHH
EVAETPAAPAAVTLEGDFPETTEKIPAMRRAIAKAMVNSKHTAPHVTLMDEIDVQALWDH
HHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHH
RKKFKEIAAEQGTKLTFLPYVVKALVSALKKYPALNTSFNEEAGEIVHKHYWNIGIAADT
HHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCEEEEEECC
DRGLLVPVVKHADRKSIFQISDEINELAVKARDGKLTADEMKGATCTISNIGSAGGQWFT
CCCEEEHHHHCCCHHHHHHHHHHHHHHEEEECCCCEEHHHHCCCEEEECCCCCCCCCEEC
PVINHPEVAILGIGRIAQKPIVKDGEIVAAPVLALSLSFDHRQIDGATGQNAMNHIKRLL
CCCCCCCEEEEECCHHHCCCCCCCCCEEEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHH
NNPELLLMEG
CCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA