Click here to switch to the map view.

The map label for this gene is ptsI

Identifier: 57651692

GI number: 57651692

Start: 1102054

End: 1103772

Strand: Direct

Name: ptsI

Synonym: SACOL1092

Alternate gene names: 57651692

Gene position: 1102054-1103772 (Clockwise)

Preceding gene: 57651691

Following gene: 57651694

Centisome position: 39.23

GC content: 35.31

Gene sequence:

>1719_bases
ATGTCTAAATTAATTAAAGGTATTGCCGCATCTGATGGTGTCGCAATTGCTAAAGCTTATTTATTAGTTGAGCCAGACTT
AACATTCGACAAAAATGAAAAAGTCACTGATGTTGAAGGAGAAGTTGCAAAGTTCAATAGCGCTATCGAAGCTTCTAAAG
TTGAGTTAACTAAAATTAGAAATAATGCAGAGGTTCAACTAGGTGCTGATAAAGCTGCTATCTTTGATGCACATTTATTA
GTTTTAGATGACCCTGAATTAATTCAACCAATCCAAGATAAGATTAAAAATGAAAACGCTAATGCTGCTACAGCATTAAC
GGATGTAACAACACAATTTGTTACAATTTTTGAATCTATGGATAACGAATACATGAAAGAACGTGCGGCTGATATTCGCG
ACGTTTCTAAACGTGTGTTATCACATATTTTAGGTGTAGAATTACCGAATCCGAGTATGATTGATGAAAGCGTTGTTATT
GTAGGGAATGACTTAACGCCATCTGATACTGCTCAATTAAATAAAGAATTCGTACAAGGTTTTGCTACAAACATTGGCGG
AAGAACAAGTCACTCTGCAATTATGAGTCGTTCTTTAGAAATTCCAGCAATTGTTGGTACAAAATCAATTACTCAAGAAG
TTAAACAAGGCGACATGATTATCGTAGATGGATTAAATGGTGATGTAATCGTTAATCCAACTGAAGATGAGTTAATCGCT
TATCAAGATAAACGTGAGCGTTATTTTGCTGACAAGAAAGAATTACAAAAACTACGTGATGCTGATACTGTTACAGTTGA
TGGTGTTCACGCAGAGCTTGCTGCAAATATTGGTACACCTAATGATTTGCCAGGTGTTATTGAAAATGGTGCACAAGGTA
TCGGCTTATATAGAACTGAGTTTTTATATATGGGTCGTGACCAAATGCCTACAGAAGAAGAACAATTTGAAGCTTATAAA
GAAGTATTAGAAGCAATGGGCGGTAAACGTGTTGTTGTACGTACTTTAGATATAGGTGGAGATAAAGAATTATCATACTT
AAACTTGCCTGAAGAAATGAATCCATTCTTAGGTTACCGTGCGATTCGTTTATGCCTTGCGCAACAAGATATTTTCAGAC
CACAGCTACGTGCATTATTACGTGCATCAGTTTATGGTAAGTTAAATATCATGTTCCCAATGGTTGCAACAATTAACGAA
TTTAGAGAAGCTAAAGCTATATTATTAGAAGAAAAAGAAAACCTTAAAAATGAAGGTCATGACATTTCGGATGATATAGA
ATTAGGAATCATGGTAGAGATACCTGCAACAGCAGCATTAGCTGATGTCTTTGCTAAAGAAGTAGATTTCTTCAGTATCG
GTACAAATGATTTAATTCAATACACATTAGCTGCTGACCGTATGTCAGAGCGTGTATCATATCTATACCAACCATATAAC
CCTTCAATCTTACGTTTAGTTAAACAAGTTATTGAAGCGTCACATAAAGAAGGTAAATGGACAGGTATGTGTGGTGAAAT
GGCTGGAGATGAAACAGCTATTCCATTATTGCTTGGTTTAGGTTTAGATGAGTTCTCTATGAGTGCAACGTCTATTCTGA
AAGCAAGAAGACAAATTAATGGTTTAAGTAAAAATGAAATGACTGAACTTGCTAACCGTGCAGTCGACTGTGCAACGCAA
GAAGAAGTTATTGAATTAGTTAACAACTACGTAAAATAA

Upstream 100 bases:

>100_bases
AAGATGCTGAAATTACAATTTATGCTGACGGTAGTGATGAATCTGACGCCATTCAAGCAATCAGTGATGTCTTATCAAAA
GAAGGATTGACTAAATAATC

Downstream 100 bases:

>100_bases
TTAATTCTTTCGAAAGACAGAATTATAAATAAACCCGAAGTATTCATTGTAAGTAATTATGAATACTTCGGGTTCTTTTT
TATGATTTATGTTAATTTCA

Product: phosphoenolpyruvate-protein phosphotransferase

Products: NA

Alternate protein names: Phosphotransferase system, enzyme I

Number of amino acids: Translated: 572; Mature: 571

Protein sequence:

>572_residues
MSKLIKGIAASDGVAIAKAYLLVEPDLTFDKNEKVTDVEGEVAKFNSAIEASKVELTKIRNNAEVQLGADKAAIFDAHLL
VLDDPELIQPIQDKIKNENANAATALTDVTTQFVTIFESMDNEYMKERAADIRDVSKRVLSHILGVELPNPSMIDESVVI
VGNDLTPSDTAQLNKEFVQGFATNIGGRTSHSAIMSRSLEIPAIVGTKSITQEVKQGDMIIVDGLNGDVIVNPTEDELIA
YQDKRERYFADKKELQKLRDADTVTVDGVHAELAANIGTPNDLPGVIENGAQGIGLYRTEFLYMGRDQMPTEEEQFEAYK
EVLEAMGGKRVVVRTLDIGGDKELSYLNLPEEMNPFLGYRAIRLCLAQQDIFRPQLRALLRASVYGKLNIMFPMVATINE
FREAKAILLEEKENLKNEGHDISDDIELGIMVEIPATAALADVFAKEVDFFSIGTNDLIQYTLAADRMSERVSYLYQPYN
PSILRLVKQVIEASHKEGKWTGMCGEMAGDETAIPLLLGLGLDEFSMSATSILKARRQINGLSKNEMTELANRAVDCATQ
EEVIELVNNYVK

Sequences:

>Translated_572_residues
MSKLIKGIAASDGVAIAKAYLLVEPDLTFDKNEKVTDVEGEVAKFNSAIEASKVELTKIRNNAEVQLGADKAAIFDAHLL
VLDDPELIQPIQDKIKNENANAATALTDVTTQFVTIFESMDNEYMKERAADIRDVSKRVLSHILGVELPNPSMIDESVVI
VGNDLTPSDTAQLNKEFVQGFATNIGGRTSHSAIMSRSLEIPAIVGTKSITQEVKQGDMIIVDGLNGDVIVNPTEDELIA
YQDKRERYFADKKELQKLRDADTVTVDGVHAELAANIGTPNDLPGVIENGAQGIGLYRTEFLYMGRDQMPTEEEQFEAYK
EVLEAMGGKRVVVRTLDIGGDKELSYLNLPEEMNPFLGYRAIRLCLAQQDIFRPQLRALLRASVYGKLNIMFPMVATINE
FREAKAILLEEKENLKNEGHDISDDIELGIMVEIPATAALADVFAKEVDFFSIGTNDLIQYTLAADRMSERVSYLYQPYN
PSILRLVKQVIEASHKEGKWTGMCGEMAGDETAIPLLLGLGLDEFSMSATSILKARRQINGLSKNEMTELANRAVDCATQ
EEVIELVNNYVK
>Mature_571_residues
SKLIKGIAASDGVAIAKAYLLVEPDLTFDKNEKVTDVEGEVAKFNSAIEASKVELTKIRNNAEVQLGADKAAIFDAHLLV
LDDPELIQPIQDKIKNENANAATALTDVTTQFVTIFESMDNEYMKERAADIRDVSKRVLSHILGVELPNPSMIDESVVIV
GNDLTPSDTAQLNKEFVQGFATNIGGRTSHSAIMSRSLEIPAIVGTKSITQEVKQGDMIIVDGLNGDVIVNPTEDELIAY
QDKRERYFADKKELQKLRDADTVTVDGVHAELAANIGTPNDLPGVIENGAQGIGLYRTEFLYMGRDQMPTEEEQFEAYKE
VLEAMGGKRVVVRTLDIGGDKELSYLNLPEEMNPFLGYRAIRLCLAQQDIFRPQLRALLRASVYGKLNIMFPMVATINEF
REAKAILLEEKENLKNEGHDISDDIELGIMVEIPATAALADVFAKEVDFFSIGTNDLIQYTLAADRMSERVSYLYQPYNP
SILRLVKQVIEASHKEGKWTGMCGEMAGDETAIPLLLGLGLDEFSMSATSILKARRQINGLSKNEMTELANRAVDCATQE
EVIELVNNYVK

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family

Homologues:

Organism=Escherichia coli, GI1788756, Length=570, Percent_Identity=50.5263157894737, Blast_Score=561, Evalue=1e-161,
Organism=Escherichia coli, GI48994992, Length=520, Percent_Identity=37.8846153846154, Blast_Score=349, Evalue=3e-97,
Organism=Escherichia coli, GI1788726, Length=533, Percent_Identity=37.8986866791745, Blast_Score=348, Evalue=6e-97,
Organism=Escherichia coli, GI1789193, Length=581, Percent_Identity=32.3580034423408, Blast_Score=280, Evalue=2e-76,
Organism=Escherichia coli, GI1787994, Length=426, Percent_Identity=27.6995305164319, Blast_Score=125, Evalue=7e-30,
Organism=Escherichia coli, GI226510935, Length=184, Percent_Identity=28.804347826087, Blast_Score=86, Evalue=5e-18,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): PT1_STAAC (Q5HH01)

Other databases:

- EMBL:   CP000046
- RefSeq:   YP_185956.1
- ProteinModelPortal:   Q5HH01
- SMR:   Q5HH01
- STRING:   Q5HH01
- EnsemblBacteria:   EBSTAT00000009828
- GeneID:   3237817
- GenomeReviews:   CP000046_GR
- KEGG:   sac:SACOL1092
- TIGR:   SACOL1092
- eggNOG:   COG1080
- GeneTree:   EBGT00050000024576
- HOGENOM:   HBG456539
- OMA:   MVEIPST
- ProtClustDB:   CLSK885128
- BioCyc:   SAUR93062:SACOL1092-MONOMER
- GO:   GO:0005737
- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813
- Gene3D:   G3DSA:3.50.30.10
- Gene3D:   G3DSA:1.10.274.10
- Gene3D:   G3DSA:3.20.20.60
- PRINTS:   PR01736
- TIGRFAMs:   TIGR01417

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; SSF47831 PEP-utilisers_N; SSF52009 PEP_mobile; SSF51621 Pyrv/PenolPyrv_Kinase_cat

EC number: =2.7.3.9

Molecular weight: Translated: 63220; Mature: 63089

Theoretical pI: Translated: 4.39; Mature: 4.39

Prosite motif: PS00742 PEP_ENZYMES_2; PS00370 PEP_ENZYMES_PHOS_SITE

Important sites: ACT_SITE 191-191 ACT_SITE 504-504 BINDING 298-298 BINDING 334-334 BINDING 433-433 BINDING 454-454 BINDING 455-455 BINDING 456-456 BINDING 457-457

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKLIKGIAASDGVAIAKAYLLVEPDLTFDKNEKVTDVEGEVAKFNSAIEASKVELTKIR
CCHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHEEHEECC
NNAEVQLGADKAAIFDAHLLVLDDPELIQPIQDKIKNENANAATALTDVTTQFVTIFESM
CCCEEEECCCCEEEEEEEEEEECCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
DNEYMKERAADIRDVSKRVLSHILGVELPNPSMIDESVVIVGNDLTPSDTAQLNKEFVQG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHH
FATNIGGRTSHSAIMSRSLEIPAIVGTKSITQEVKQGDMIIVDGLNGDVIVNPTEDELIA
HHHHCCCCCHHHHHHHCCCCCCEEECCHHHHHHHHCCCEEEEECCCCCEEECCCCCCCEE
YQDKRERYFADKKELQKLRDADTVTVDGVHAELAANIGTPNDLPGVIENGAQGIGLYRTE
EHHHHHHHCCCHHHHHHHHCCCCEEECCHHHHHHHCCCCCCCCCHHHHCCCCCCCCEEHH
FLYMGRDQMPTEEEQFEAYKEVLEAMGGKRVVVRTLDIGGDKELSYLNLPEEMNPFLGYR
EEEECCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCEEECCCHHCCHHHHHH
AIRLCLAQQDIFRPQLRALLRASVYGKLNIMFPMVATINEFREAKAILLEEKENLKNEGH
HHHHHHHHHHHHHHHHHHHHHHHHHCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DISDDIELGIMVEIPATAALADVFAKEVDFFSIGTNDLIQYTLAADRMSERVSYLYQPYN
CCCCCCEEEEEEECCCHHHHHHHHHHHCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCCC
PSILRLVKQVIEASHKEGKWTGMCGEMAGDETAIPLLLGLGLDEFSMSATSILKARRQIN
CHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHC
GLSKNEMTELANRAVDCATQEEVIELVNNYVK
CCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
SKLIKGIAASDGVAIAKAYLLVEPDLTFDKNEKVTDVEGEVAKFNSAIEASKVELTKIR
CHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHEEHEECC
NNAEVQLGADKAAIFDAHLLVLDDPELIQPIQDKIKNENANAATALTDVTTQFVTIFESM
CCCEEEECCCCEEEEEEEEEEECCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
DNEYMKERAADIRDVSKRVLSHILGVELPNPSMIDESVVIVGNDLTPSDTAQLNKEFVQG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHH
FATNIGGRTSHSAIMSRSLEIPAIVGTKSITQEVKQGDMIIVDGLNGDVIVNPTEDELIA
HHHHCCCCCHHHHHHHCCCCCCEEECCHHHHHHHHCCCEEEEECCCCCEEECCCCCCCEE
YQDKRERYFADKKELQKLRDADTVTVDGVHAELAANIGTPNDLPGVIENGAQGIGLYRTE
EHHHHHHHCCCHHHHHHHHCCCCEEECCHHHHHHHCCCCCCCCCHHHHCCCCCCCCEEHH
FLYMGRDQMPTEEEQFEAYKEVLEAMGGKRVVVRTLDIGGDKELSYLNLPEEMNPFLGYR
EEEECCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCEEECCCHHCCHHHHHH
AIRLCLAQQDIFRPQLRALLRASVYGKLNIMFPMVATINEFREAKAILLEEKENLKNEGH
HHHHHHHHHHHHHHHHHHHHHHHHHCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DISDDIELGIMVEIPATAALADVFAKEVDFFSIGTNDLIQYTLAADRMSERVSYLYQPYN
CCCCCCEEEEEEECCCHHHHHHHHHHHCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCCC
PSILRLVKQVIEASHKEGKWTGMCGEMAGDETAIPLLLGLGLDEFSMSATSILKARRQIN
CHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHC
GLSKNEMTELANRAVDCATQEEVIELVNNYVK
CCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA