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The map label for this gene is purD

Identifier: 57651683

GI number: 57651683

Start: 1092909

End: 1094156

Strand: Direct

Name: purD

Synonym: SACOL1083

Alternate gene names: 57651683

Gene position: 1092909-1094156 (Clockwise)

Preceding gene: 57651682

Following gene: 57651688

Centisome position: 38.9

GC content: 35.74

Gene sequence:

>1248_bases
ATGAATGTATTAGTAATTGGTGCTGGTGGACGAGAACATGCACTTGCATATAAACTTAATCAATCGAATCTAGTTAAACA
AGTGTTTGTCATTCCAGGTAATGAGGCAATGACACCTATAGCTGAAGTACACACTGAAATTTCAGAACCTGATCATCAAG
CGATACTAGATTTTGCTAAACGGCAAAATGTTGATTGGGTAGTTATAGGTCCAGAACAGCCGCTAATTGATGGATTAGCA
GACATTTTACGAGCGAATGGTTTCAAAGTGTTTGGTCCAAATAAGCAAGCAGCTCAAATCGAAGGCTCAAAATTATTTGC
TAAAAAGATAATGGAAAAATATAATATTCCAACTGCTGATTATAAAGAAGTTGAGCGAAAAAAGGATGCTTTAACATATA
TTGAAAACTGTGAATTGCCCGTTGTTGTCAAGAAAGATGGGTTAGCTGCTGGGAAAGGCGTTATTATTGCAGATACTATT
GAAGCAGCCAGAAGTGCTATTGAGATTATGTATGGTGATGAAGAAGAAGGTACTGTTGTATTTGAAACGTTTTTAGAAGG
TGAAGAGTTCTCGCTAATGACATTTGTTAATGGTGATTTAGCAGTACCTTTCGACTGTATTGCACAAGATCATAAACGCG
CATTTGATCATGATGAAGGACCAAATACTGGTGGTATGGGGGCTTATTGTCCAGTACCACATATTAGTGACGATGTTTTA
AAACTTACAAATGAAACAATTGCACAACCCATTGCAAAGGCAATGCTTAATGAAGGTTATCAATTCTTCGGTGTATTATA
CATTGGTGCTATTTTAACTAAAGATGGTCCAAAAGTAATAGAATTTAATGCCCGTTTTGGTGATCCTGAAGCTCAAGTAT
TATTAAGTCGCATGGAAAGTGATTTAATGCAGCATATTATTGATTTAGATGAAGGAAAACGTACTGAATTCAAATGGAAA
AATGAATCTATTGTAGGGGTCATGTTGGCATCAAAAGGATATCCTGATGCATATGAAAAAGGGCATAAAGTAAGTGGCTT
TGATTTAAATGAAAACTATTTTGTTAGTGGATTAAAGAAGCAAGGTGATACCTTTGTTACTTCAGGTGGTAGAGTTATAC
TTGCCATCGGAAAAGGTGACAATGTACAAGATGCACAGCGAGACGCATACAAAAAAGTATCACAAATACAAAGTGACCAT
TTATTCTATCGTCATGACATTGCGAATAAAGCACTACAACTTAAATAA

Upstream 100 bases:

>100_bases
AGATCAAGATTCAATTGATATGGCTAATAAACATGGTATTGCAATGGTAGTCACAGGCACTCGACATTTTAAACACTAAT
TTTAAAGGGGCGTAACATAA

Downstream 100 bases:

>100_bases
GTAAATTTAAAATACTAAGATTAGCTATGAACGAATCTATAACGATAGATTTTTTCATAGCTTTTTTAGTTGTAGAGTCT
AGGACATTGATTTCTGTACC

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase

Number of amino acids: Translated: 415; Mature: 415

Protein sequence:

>415_residues
MNVLVIGAGGREHALAYKLNQSNLVKQVFVIPGNEAMTPIAEVHTEISEPDHQAILDFAKRQNVDWVVIGPEQPLIDGLA
DILRANGFKVFGPNKQAAQIEGSKLFAKKIMEKYNIPTADYKEVERKKDALTYIENCELPVVVKKDGLAAGKGVIIADTI
EAARSAIEIMYGDEEEGTVVFETFLEGEEFSLMTFVNGDLAVPFDCIAQDHKRAFDHDEGPNTGGMGAYCPVPHISDDVL
KLTNETIAQPIAKAMLNEGYQFFGVLYIGAILTKDGPKVIEFNARFGDPEAQVLLSRMESDLMQHIIDLDEGKRTEFKWK
NESIVGVMLASKGYPDAYEKGHKVSGFDLNENYFVSGLKKQGDTFVTSGGRVILAIGKGDNVQDAQRDAYKKVSQIQSDH
LFYRHDIANKALQLK

Sequences:

>Translated_415_residues
MNVLVIGAGGREHALAYKLNQSNLVKQVFVIPGNEAMTPIAEVHTEISEPDHQAILDFAKRQNVDWVVIGPEQPLIDGLA
DILRANGFKVFGPNKQAAQIEGSKLFAKKIMEKYNIPTADYKEVERKKDALTYIENCELPVVVKKDGLAAGKGVIIADTI
EAARSAIEIMYGDEEEGTVVFETFLEGEEFSLMTFVNGDLAVPFDCIAQDHKRAFDHDEGPNTGGMGAYCPVPHISDDVL
KLTNETIAQPIAKAMLNEGYQFFGVLYIGAILTKDGPKVIEFNARFGDPEAQVLLSRMESDLMQHIIDLDEGKRTEFKWK
NESIVGVMLASKGYPDAYEKGHKVSGFDLNENYFVSGLKKQGDTFVTSGGRVILAIGKGDNVQDAQRDAYKKVSQIQSDH
LFYRHDIANKALQLK
>Mature_415_residues
MNVLVIGAGGREHALAYKLNQSNLVKQVFVIPGNEAMTPIAEVHTEISEPDHQAILDFAKRQNVDWVVIGPEQPLIDGLA
DILRANGFKVFGPNKQAAQIEGSKLFAKKIMEKYNIPTADYKEVERKKDALTYIENCELPVVVKKDGLAAGKGVIIADTI
EAARSAIEIMYGDEEEGTVVFETFLEGEEFSLMTFVNGDLAVPFDCIAQDHKRAFDHDEGPNTGGMGAYCPVPHISDDVL
KLTNETIAQPIAKAMLNEGYQFFGVLYIGAILTKDGPKVIEFNARFGDPEAQVLLSRMESDLMQHIIDLDEGKRTEFKWK
NESIVGVMLASKGYPDAYEKGHKVSGFDLNENYFVSGLKKQGDTFVTSGGRVILAIGKGDNVQDAQRDAYKKVSQIQSDH
LFYRHDIANKALQLK

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Homo sapiens, GI28416899, Length=426, Percent_Identity=43.4272300469484, Blast_Score=330, Evalue=1e-90,
Organism=Homo sapiens, GI4503915, Length=426, Percent_Identity=43.4272300469484, Blast_Score=329, Evalue=3e-90,
Organism=Homo sapiens, GI209869995, Length=426, Percent_Identity=43.4272300469484, Blast_Score=329, Evalue=3e-90,
Organism=Homo sapiens, GI209869993, Length=426, Percent_Identity=43.4272300469484, Blast_Score=329, Evalue=3e-90,
Organism=Escherichia coli, GI1790438, Length=428, Percent_Identity=44.6261682242991, Blast_Score=340, Evalue=9e-95,
Organism=Caenorhabditis elegans, GI17567511, Length=423, Percent_Identity=39.2434988179669, Blast_Score=253, Evalue=1e-67,
Organism=Saccharomyces cerevisiae, GI6321203, Length=447, Percent_Identity=38.255033557047, Blast_Score=280, Evalue=3e-76,
Organism=Drosophila melanogaster, GI62471619, Length=432, Percent_Identity=40.9722222222222, Blast_Score=287, Evalue=1e-77,
Organism=Drosophila melanogaster, GI24582400, Length=432, Percent_Identity=40.9722222222222, Blast_Score=285, Evalue=4e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PUR2_STAAC (Q5HH10)

Other databases:

- EMBL:   CP000046
- RefSeq:   YP_185947.1
- ProteinModelPortal:   Q5HH10
- SMR:   Q5HH10
- STRING:   Q5HH10
- EnsemblBacteria:   EBSTAT00000010666
- GeneID:   3236107
- GenomeReviews:   CP000046_GR
- KEGG:   sac:SACOL1083
- TIGR:   SACOL1083
- eggNOG:   COG0151
- GeneTree:   EBGT00050000023921
- HOGENOM:   HBG404060
- OMA:   GVPIVIK
- ProtClustDB:   PRK13790
- BioCyc:   SAUR93062:SACOL1083-MONOMER
- HAMAP:   MF_00138
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.90.600.10
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR00877

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N; SSF52440 PreATP-grasp-like; SSF51246 Rudmnt_hyb_motif

EC number: =6.3.4.13

Molecular weight: Translated: 45860; Mature: 45860

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS50975 ATP_GRASP; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVLVIGAGGREHALAYKLNQSNLVKQVFVIPGNEAMTPIAEVHTEISEPDHQAILDFAK
CCEEEEECCCCCCEEEEEECCCCCEEEEEEECCCCCCCCHHHHHHHCCCCHHHHHHHHHH
RQNVDWVVIGPEQPLIDGLADILRANGFKVFGPNKQAAQIEGSKLFAKKIMEKYNIPTAD
HCCCCEEEECCCCCHHHHHHHHHHCCCEEEECCCCCCEEECCHHHHHHHHHHHHCCCCCC
YKEVERKKDALTYIENCELPVVVKKDGLAAGKGVIIADTIEAARSAIEIMYGDEEEGTVV
HHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCEEEEHHHHHHHHHEEEEECCCCCCEEE
FETFLEGEEFSLMTFVNGDLAVPFDCIAQDHKRAFDHDEGPNTGGMGAYCPVPHISDDVL
EEEECCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHH
KLTNETIAQPIAKAMLNEGYQFFGVLYIGAILTKDGPKVIEFNARFGDPEAQVLLSRMES
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHH
DLMQHIIDLDEGKRTEFKWKNESIVGVMLASKGYPDAYEKGHKVSGFDLNENYFVSGLKK
HHHHHHHCCCCCCCCCEEECCCCEEEEEEECCCCCHHHHCCCCCCCEECCCCCEEECHHC
QGDTFVTSGGRVILAIGKGDNVQDAQRDAYKKVSQIQSDHLFYRHDIANKALQLK
CCCEEEECCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCEEEEHHHHCCCEECC
>Mature Secondary Structure
MNVLVIGAGGREHALAYKLNQSNLVKQVFVIPGNEAMTPIAEVHTEISEPDHQAILDFAK
CCEEEEECCCCCCEEEEEECCCCCEEEEEEECCCCCCCCHHHHHHHCCCCHHHHHHHHHH
RQNVDWVVIGPEQPLIDGLADILRANGFKVFGPNKQAAQIEGSKLFAKKIMEKYNIPTAD
HCCCCEEEECCCCCHHHHHHHHHHCCCEEEECCCCCCEEECCHHHHHHHHHHHHCCCCCC
YKEVERKKDALTYIENCELPVVVKKDGLAAGKGVIIADTIEAARSAIEIMYGDEEEGTVV
HHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCEEEEHHHHHHHHHEEEEECCCCCCEEE
FETFLEGEEFSLMTFVNGDLAVPFDCIAQDHKRAFDHDEGPNTGGMGAYCPVPHISDDVL
EEEECCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHH
KLTNETIAQPIAKAMLNEGYQFFGVLYIGAILTKDGPKVIEFNARFGDPEAQVLLSRMES
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHH
DLMQHIIDLDEGKRTEFKWKNESIVGVMLASKGYPDAYEKGHKVSGFDLNENYFVSGLKK
HHHHHHHCCCCCCCCCEEECCCCEEEEEEECCCCCHHHHCCCCCCCEECCCCCEEECHHC
QGDTFVTSGGRVILAIGKGDNVQDAQRDAYKKVSQIQSDHLFYRHDIANKALQLK
CCCEEEECCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCEEEEHHHHCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA