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The map label for this gene is purK

Identifier: 57651674

GI number: 57651674

Start: 1083391

End: 1084515

Strand: Direct

Name: purK

Synonym: SACOL1074

Alternate gene names: 57651674

Gene position: 1083391-1084515 (Clockwise)

Preceding gene: 57651673

Following gene: 57651675

Centisome position: 38.56

GC content: 32.62

Gene sequence:

>1125_bases
ATGAACTTCAATAAATTAAAGTTTGGTGCGACTATTGGCATTATTGGTGGTGGTCAGCTTGGAAAGATGATGGCACAATC
AGCTCAAAAAATGGGTTATAAAGTGGTTGTATTGGATCCTTCTGAAGATTGTCCATGTAGATACGTTGCACACGAATTTA
TACAAGCCAAGTATGACGATGAAAAGGCACTCAATCAATTAGGACAAAAATGTGATGTGATTACTTATGAATTTGAAAAC
ATTTCAGCCCAACAATTAAAACTATTATGTGAAAAGTACAATATTCCGCAAGGTTACCAAGCTATACAGTTATTACAAGA
TCGCTTAACTGAAAAAGAAACATTAAAAAGTGCTGGTACCAAAGTTGTCCCGTTCATTTCAGTAAAAGAATCTACAGATA
TTGACAAAGCAATTGAAACATTAGGATATCCTTTTATTGTAAAAACTAGATTTGGTGGCTACGATGGCAAAGGTCAAGTT
TTAATTAACAACGAAAAAGACTTACAAGAAGGTTTTAAATTAATTGAAACTAGTGAATGCGTAGCTGAAAAATATTTGAA
TATCAAGAAAGAAGTATCTCTTACTGTTACAAGAGGAAACAACAATCAAATCACTTTTTTCCCATTACAAGAAAATGAGC
ATAGAAATCAAATACTTTTCAAAACAATTGTTCCAGCGAGAATAGATAAAACAGCTGAGGCGAAAGAGCAAGTTAATAAA
ATTATCCAATCGATTCATTTCATTGGAACATTTACAGTTGAATTTTTTATAGATAGTAACAACCAATTGTATGTGAACGA
GATAGCACCAAGGCCTCACAATTCCGGACATTATTCAATTGAAGCATGTGATTATTCACAATTTGATACTCATATTTTAG
CAGTTACCGGACAATCATTACCAAATTCAATTGAATTATTAAAGCCAGCAGTCATGATGAACTTACTAGGTAAAGATTTA
GATTTATTGGAAAATGAATTTAATGAACATCCAGAGTGGCACTTACATATTTATGGTAAGTCTGAGCGTAAAGATAGCAG
AAAAATGGGGCATATGACTGTACTAACGAATGATGTAAACCAAACTGAACAAGATATGTACGCTAAATTTGAGGGGAGTA
ATTAA

Upstream 100 bases:

>100_bases
ATACTTGCAGCAAGAATGTTAAGTATTCAAAATCCTTCTTTAGTTGAAAAACTAAATCAGTATGAATCTTCGTTAATTCA
AAAAGTGGAGGACATGCAAA

Downstream 100 bases:

>100_bases
AGCATGACATTATTATATGAAGGAAAAGCGAAGCGCATTTTCTCAACAAATCAAGAAAATGAATTAAGAGTTGAATATAA
AGATGAAGTTACTGCTGGAA

Product: phosphoribosylaminoimidazole carboxylase ATPase subunit

Products: NA

Alternate protein names: N5-CAIR synthase; 5-(carboxyamino)imidazole ribonucleotide synthetase

Number of amino acids: Translated: 374; Mature: 374

Protein sequence:

>374_residues
MNFNKLKFGATIGIIGGGQLGKMMAQSAQKMGYKVVVLDPSEDCPCRYVAHEFIQAKYDDEKALNQLGQKCDVITYEFEN
ISAQQLKLLCEKYNIPQGYQAIQLLQDRLTEKETLKSAGTKVVPFISVKESTDIDKAIETLGYPFIVKTRFGGYDGKGQV
LINNEKDLQEGFKLIETSECVAEKYLNIKKEVSLTVTRGNNNQITFFPLQENEHRNQILFKTIVPARIDKTAEAKEQVNK
IIQSIHFIGTFTVEFFIDSNNQLYVNEIAPRPHNSGHYSIEACDYSQFDTHILAVTGQSLPNSIELLKPAVMMNLLGKDL
DLLENEFNEHPEWHLHIYGKSERKDSRKMGHMTVLTNDVNQTEQDMYAKFEGSN

Sequences:

>Translated_374_residues
MNFNKLKFGATIGIIGGGQLGKMMAQSAQKMGYKVVVLDPSEDCPCRYVAHEFIQAKYDDEKALNQLGQKCDVITYEFEN
ISAQQLKLLCEKYNIPQGYQAIQLLQDRLTEKETLKSAGTKVVPFISVKESTDIDKAIETLGYPFIVKTRFGGYDGKGQV
LINNEKDLQEGFKLIETSECVAEKYLNIKKEVSLTVTRGNNNQITFFPLQENEHRNQILFKTIVPARIDKTAEAKEQVNK
IIQSIHFIGTFTVEFFIDSNNQLYVNEIAPRPHNSGHYSIEACDYSQFDTHILAVTGQSLPNSIELLKPAVMMNLLGKDL
DLLENEFNEHPEWHLHIYGKSERKDSRKMGHMTVLTNDVNQTEQDMYAKFEGSN
>Mature_374_residues
MNFNKLKFGATIGIIGGGQLGKMMAQSAQKMGYKVVVLDPSEDCPCRYVAHEFIQAKYDDEKALNQLGQKCDVITYEFEN
ISAQQLKLLCEKYNIPQGYQAIQLLQDRLTEKETLKSAGTKVVPFISVKESTDIDKAIETLGYPFIVKTRFGGYDGKGQV
LINNEKDLQEGFKLIETSECVAEKYLNIKKEVSLTVTRGNNNQITFFPLQENEHRNQILFKTIVPARIDKTAEAKEQVNK
IIQSIHFIGTFTVEFFIDSNNQLYVNEIAPRPHNSGHYSIEACDYSQFDTHILAVTGQSLPNSIELLKPAVMMNLLGKDL
DLLENEFNEHPEWHLHIYGKSERKDSRKMGHMTVLTNDVNQTEQDMYAKFEGSN

Specific function: Catalyzes the ATP-dependent conversion of 5- aminoimidazole ribonucleotide (AIR) and HCO(3)(-) to N5- carboxyaminoimidazole ribonucleotide (N5-CAIR)

COG id: COG0026

COG function: function code F; Phosphoribosylaminoimidazole carboxylase (NCAIR synthetase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1786733, Length=359, Percent_Identity=28.6908077994429, Blast_Score=152, Evalue=4e-38,
Organism=Escherichia coli, GI1788155, Length=373, Percent_Identity=24.1286863270777, Blast_Score=90, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6324702, Length=381, Percent_Identity=31.2335958005249, Blast_Score=208, Evalue=1e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PURK_STAAC (Q5HH19)

Other databases:

- EMBL:   CP000046
- RefSeq:   YP_185938.1
- ProteinModelPortal:   Q5HH19
- SMR:   Q5HH19
- STRING:   Q5HH19
- EnsemblBacteria:   EBSTAT00000009161
- GeneID:   3237669
- GenomeReviews:   CP000046_GR
- KEGG:   sac:SACOL1074
- TIGR:   SACOL1074
- eggNOG:   COG0026
- GeneTree:   EBGT00050000023923
- HOGENOM:   HBG516369
- OMA:   LAVEMFV
- ProtClustDB:   PRK06019
- BioCyc:   SAUR93062:SACOL1074-MONOMER
- InterPro:   IPR005875
- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR006140
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR011054
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- PANTHER:   PTHR23047:SF1
- TIGRFAMs:   TIGR01161

Pfam domain/function: PF02826 2-Hacid_dh_C; PF02222 ATP-grasp; SSF52440 PreATP-grasp-like; SSF51246 Rudmnt_hyb_motif

EC number: =6.3.4.18

Molecular weight: Translated: 42494; Mature: 42494

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: PS50975 ATP_GRASP

Important sites: BINDING 108-108 BINDING 148-148 BINDING 159-159 BINDING 191-191

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFNKLKFGATIGIIGGGQLGKMMAQSAQKMGYKVVVLDPSEDCPCRYVAHEFIQAKYDD
CCCCEEEECEEEEEECCCHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHCCCH
EKALNQLGQKCDVITYEFENISAQQLKLLCEKYNIPQGYQAIQLLQDRLTEKETLKSAGT
HHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCC
KVVPFISVKESTDIDKAIETLGYPFIVKTRFGGYDGKGQVLINNEKDLQEGFKLIETSEC
EEEEEEEECCCCCHHHHHHHCCCCEEEEEECCCCCCCCCEEECCCHHHHHHHHHHHHHHH
VAEKYLNIKKEVSLTVTRGNNNQITFFPLQENEHRNQILFKTIVPARIDKTAEAKEQVNK
HHHHHHCCCEEEEEEEEECCCCEEEEEECCCCCCCCEEEEEECCCHHCCCHHHHHHHHHH
IIQSIHFIGTFTVEFFIDSNNQLYVNEIAPRPHNSGHYSIEACDYSQFDTHILAVTGQSL
HHHHHHHHEEEEEEEEECCCCEEEEEECCCCCCCCCCEEEEECCCCCCCCEEEEEECCCC
PNSIELLKPAVMMNLLGKDLDLLENEFNEHPEWHLHIYGKSERKDSRKMGHMTVLTNDVN
CCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCEEEEEEECCCCCHHHHCCCEEEEECCCC
QTEQDMYAKFEGSN
HHHHHHEEEECCCC
>Mature Secondary Structure
MNFNKLKFGATIGIIGGGQLGKMMAQSAQKMGYKVVVLDPSEDCPCRYVAHEFIQAKYDD
CCCCEEEECEEEEEECCCHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHCCCH
EKALNQLGQKCDVITYEFENISAQQLKLLCEKYNIPQGYQAIQLLQDRLTEKETLKSAGT
HHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCC
KVVPFISVKESTDIDKAIETLGYPFIVKTRFGGYDGKGQVLINNEKDLQEGFKLIETSEC
EEEEEEEECCCCCHHHHHHHCCCCEEEEEECCCCCCCCCEEECCCHHHHHHHHHHHHHHH
VAEKYLNIKKEVSLTVTRGNNNQITFFPLQENEHRNQILFKTIVPARIDKTAEAKEQVNK
HHHHHHCCCEEEEEEEEECCCCEEEEEECCCCCCCCEEEEEECCCHHCCCHHHHHHHHHH
IIQSIHFIGTFTVEFFIDSNNQLYVNEIAPRPHNSGHYSIEACDYSQFDTHILAVTGQSL
HHHHHHHHEEEEEEEEECCCCEEEEEECCCCCCCCCCEEEEECCCCCCCCEEEEEECCCC
PNSIELLKPAVMMNLLGKDLDLLENEFNEHPEWHLHIYGKSERKDSRKMGHMTVLTNDVN
CCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCEEEEEEECCCCCHHHHCCCEEEEECCCC
QTEQDMYAKFEGSN
HHHHHHEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA