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The map label for this gene is purA

Identifier: 57651126

GI number: 57651126

Start: 22465

End: 23748

Strand: Direct

Name: purA

Synonym: SACOL0018

Alternate gene names: 57651126

Gene position: 22465-23748 (Clockwise)

Preceding gene: 57651125

Following gene: 57651127

Centisome position: 0.8

GC content: 36.37

Gene sequence:

>1284_bases
ATGTCATCAATCGTAGTAGTTGGGACACAATGGGGAGACGAAGGAAAAGGAAAAATAACGGATTTCTTGGCAGAACAGTC
AGATGTTATCGCGCGTTTTTCAGGTGGTAATAATGCAGGCCATACCATTCAATTTGGCGGAGAAACATATAAATTACATT
TAGTACCATCTGGTATCTTTTACAAAGACAAATTAGCGGTAATCGGTAACGGAGTCGTTGTTGATCCAGTTGCACTATTG
AAAGAATTAGACGGATTAAATGAACGTGGCATTCCTACAAGTAATTTACGTATATCTAATCGTGCGCAAGTGATTTTACC
ATATCACTTAGCACAAGATGAATATGAAGAACGTTTACGTGGTGACAATAAGATTGGTACAACTAAAAAAGGTATCGGTC
CAGCATATGTAGACAAAGTTCAACGTATCGGTATTCGTATGGCAGATTTACTTGAAAAAGAAACATTCGAAAGATTATTA
AAATCAAACATTGAATATAAACAAGCATATTTCAAAGGTATGTTTAACGAAACATGTCCATCATTTGATGATATCTTTGA
AGAATATTATGCAGCAGGTCAACGTCTAAAAGAATTTGTAACAGACACATCAAAAATCTTAGACGATGCATTTGTAGCAG
ATGAAAAGGTACTTTTCGAAGGTGCGCAAGGTGTAATGTTAGATATCGACCATGGTACATATCCATTCGTTACATCAAGT
AATCCAATTGCAGGTAACGTTACTGTTGGTACAGGTGTAGGTCCTACATTCGTTTCAAAGGTAATTGGTGTATGTAAAGC
TTATACATCACGTGTTGGTGATGGTCCATTCCCTACTGAATTATTCGATGAAGATGGACATCATATTAGAGAGGTTGGTC
GTGAATACGGTACAACAACAGGACGTCCACGTCGTGTAGGTTGGTTTGATTCAGTTGTATTACGTCACTCTCGTCGTGTA
AGTGGTATTACAGATTTATCTATTAACTCAATCGATGTTTTAACAGGCCTAGACACAGTGAAAATCTGTACAGCTTATGA
ATTAGACGGTAAAGAAATTACTGAGTACCCAGCAAACTTAGATCAATTAAAACGTTGTAAACCAATCTTTGAAGAGTTAC
CAGGTTGGACAGAAGACGTAACAAATGTGCGTACTTTAGAAGAATTACCTGAAAATGCACGTAAATATTTAGAGCGTATT
TCAGAATTATGTAATGTACAAATTTCTATCTTCTCAGTTGGTCCAGATAGAGAACAAACAAACCTATTAAAAGAATTGTG
GTAG

Upstream 100 bases:

>100_bases
TAAAAAGGCCGAAAATCAATGTTCGATTTTTATTTGCATTATGGTCTCGATATTGGTAGAATATCAAATGGTTAAATGAG
AAAAACTTGGAGGTGCTCAC

Downstream 100 bases:

>100_bases
AACTTTATATAAGTCATACACAATGATTATAAATACATGAGCCTTCTATCTTTATTGGTAGGAGGCTTTTGTTATGCTTG
CTTCTGTATCGATTCGATTA

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MSSIVVVGTQWGDEGKGKITDFLAEQSDVIARFSGGNNAGHTIQFGGETYKLHLVPSGIFYKDKLAVIGNGVVVDPVALL
KELDGLNERGIPTSNLRISNRAQVILPYHLAQDEYEERLRGDNKIGTTKKGIGPAYVDKVQRIGIRMADLLEKETFERLL
KSNIEYKQAYFKGMFNETCPSFDDIFEEYYAAGQRLKEFVTDTSKILDDAFVADEKVLFEGAQGVMLDIDHGTYPFVTSS
NPIAGNVTVGTGVGPTFVSKVIGVCKAYTSRVGDGPFPTELFDEDGHHIREVGREYGTTTGRPRRVGWFDSVVLRHSRRV
SGITDLSINSIDVLTGLDTVKICTAYELDGKEITEYPANLDQLKRCKPIFEELPGWTEDVTNVRTLEELPENARKYLERI
SELCNVQISIFSVGPDREQTNLLKELW

Sequences:

>Translated_427_residues
MSSIVVVGTQWGDEGKGKITDFLAEQSDVIARFSGGNNAGHTIQFGGETYKLHLVPSGIFYKDKLAVIGNGVVVDPVALL
KELDGLNERGIPTSNLRISNRAQVILPYHLAQDEYEERLRGDNKIGTTKKGIGPAYVDKVQRIGIRMADLLEKETFERLL
KSNIEYKQAYFKGMFNETCPSFDDIFEEYYAAGQRLKEFVTDTSKILDDAFVADEKVLFEGAQGVMLDIDHGTYPFVTSS
NPIAGNVTVGTGVGPTFVSKVIGVCKAYTSRVGDGPFPTELFDEDGHHIREVGREYGTTTGRPRRVGWFDSVVLRHSRRV
SGITDLSINSIDVLTGLDTVKICTAYELDGKEITEYPANLDQLKRCKPIFEELPGWTEDVTNVRTLEELPENARKYLERI
SELCNVQISIFSVGPDREQTNLLKELW
>Mature_426_residues
SSIVVVGTQWGDEGKGKITDFLAEQSDVIARFSGGNNAGHTIQFGGETYKLHLVPSGIFYKDKLAVIGNGVVVDPVALLK
ELDGLNERGIPTSNLRISNRAQVILPYHLAQDEYEERLRGDNKIGTTKKGIGPAYVDKVQRIGIRMADLLEKETFERLLK
SNIEYKQAYFKGMFNETCPSFDDIFEEYYAAGQRLKEFVTDTSKILDDAFVADEKVLFEGAQGVMLDIDHGTYPFVTSSN
PIAGNVTVGTGVGPTFVSKVIGVCKAYTSRVGDGPFPTELFDEDGHHIREVGREYGTTTGRPRRVGWFDSVVLRHSRRVS
GITDLSINSIDVLTGLDTVKICTAYELDGKEITEYPANLDQLKRCKPIFEELPGWTEDVTNVRTLEELPENARKYLERIS
ELCNVQISIFSVGPDREQTNLLKELW

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family

Homologues:

Organism=Homo sapiens, GI22748717, Length=422, Percent_Identity=40.9952606635071, Blast_Score=310, Evalue=1e-84,
Organism=Homo sapiens, GI34577063, Length=431, Percent_Identity=40.3712296983759, Blast_Score=305, Evalue=6e-83,
Organism=Homo sapiens, GI40316944, Length=392, Percent_Identity=39.5408163265306, Blast_Score=270, Evalue=2e-72,
Organism=Escherichia coli, GI1790620, Length=426, Percent_Identity=46.9483568075117, Blast_Score=409, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI25146555, Length=426, Percent_Identity=41.3145539906103, Blast_Score=315, Evalue=4e-86,
Organism=Caenorhabditis elegans, GI25146553, Length=426, Percent_Identity=41.3145539906103, Blast_Score=314, Evalue=5e-86,
Organism=Saccharomyces cerevisiae, GI6324109, Length=438, Percent_Identity=40.4109589041096, Blast_Score=327, Evalue=2e-90,
Organism=Drosophila melanogaster, GI21356033, Length=423, Percent_Identity=38.0614657210402, Blast_Score=293, Evalue=1e-79,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PURA_STAAC (Q5HJX8)

Other databases:

- EMBL:   CP000046
- RefSeq:   YP_184929.1
- ProteinModelPortal:   Q5HJX8
- SMR:   Q5HJX8
- STRING:   Q5HJX8
- EnsemblBacteria:   EBSTAT00000010597
- GeneID:   3236538
- GenomeReviews:   CP000046_GR
- KEGG:   sac:SACOL0018
- TIGR:   SACOL0018
- eggNOG:   COG0104
- GeneTree:   EBGT00050000025160
- HOGENOM:   HBG658237
- OMA:   DYVVRYQ
- ProtClustDB:   PRK01117
- BioCyc:   SAUR93062:SACOL0018-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00011
- InterPro:   IPR018220
- InterPro:   IPR001114
- PANTHER:   PTHR11846
- SMART:   SM00788
- TIGRFAMs:   TIGR00184

Pfam domain/function: PF00709 Adenylsucc_synt

EC number: =6.3.4.4

Molecular weight: Translated: 47579; Mature: 47448

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1; PS00513 ADENYLOSUCCIN_SYN_2; PS00178 AA_TRNA_LIGASE_I

Important sites: ACT_SITE 13-13 ACT_SITE 41-41 BINDING 128-128 BINDING 142-142 BINDING 223-223 BINDING 238-238 BINDING 302-302 BINDING 304-304

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSIVVVGTQWGDEGKGKITDFLAEQSDVIARFSGGNNAGHTIQFGGETYKLHLVPSGIF
CCCEEEEEECCCCCCCCHHHHHHHCCCCEEEEECCCCCCCCEEEECCCEEEEEEEECCCE
YKDKLAVIGNGVVVDPVALLKELDGLNERGIPTSNLRISNRAQVILPYHLAQDEYEERLR
EECCEEEEECCEEECHHHHHHHHCCCCCCCCCCCCEEECCCCEEEEEHHHHHHHHHHHHC
GDNKIGTTKKGIGPAYVDKVQRIGIRMADLLEKETFERLLKSNIEYKQAYFKGMFNETCP
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCC
SFDDIFEEYYAAGQRLKEFVTDTSKILDDAFVADEKVLFEGAQGVMLDIDHGTYPFVTSS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCEEEEECCCCCCEEECC
NPIAGNVTVGTGVGPTFVSKVIGVCKAYTSRVGDGPFPTELFDEDGHHIREVGREYGTTT
CCCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCHHHHHHHHHHCCCC
GRPRRVGWFDSVVLRHSRRVSGITDLSINSIDVLTGLDTVKICTAYELDGKEITEYPANL
CCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCEEEEEEEECCCCHHHHCCCCH
DQLKRCKPIFEELPGWTEDVTNVRTLEELPENARKYLERISELCNVQISIFSVGPDREQT
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCHHHH
NLLKELW
HHHHHHC
>Mature Secondary Structure 
SSIVVVGTQWGDEGKGKITDFLAEQSDVIARFSGGNNAGHTIQFGGETYKLHLVPSGIF
CCEEEEEECCCCCCCCHHHHHHHCCCCEEEEECCCCCCCCEEEECCCEEEEEEEECCCE
YKDKLAVIGNGVVVDPVALLKELDGLNERGIPTSNLRISNRAQVILPYHLAQDEYEERLR
EECCEEEEECCEEECHHHHHHHHCCCCCCCCCCCCEEECCCCEEEEEHHHHHHHHHHHHC
GDNKIGTTKKGIGPAYVDKVQRIGIRMADLLEKETFERLLKSNIEYKQAYFKGMFNETCP
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCC
SFDDIFEEYYAAGQRLKEFVTDTSKILDDAFVADEKVLFEGAQGVMLDIDHGTYPFVTSS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCEEEEECCCCCCEEECC
NPIAGNVTVGTGVGPTFVSKVIGVCKAYTSRVGDGPFPTELFDEDGHHIREVGREYGTTT
CCCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCHHHHHHHHHHCCCC
GRPRRVGWFDSVVLRHSRRVSGITDLSINSIDVLTGLDTVKICTAYELDGKEITEYPANL
CCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCEEEEEEEECCCCHHHHCCCCH
DQLKRCKPIFEELPGWTEDVTNVRTLEELPENARKYLERISELCNVQISIFSVGPDREQT
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCHHHH
NLLKELW
HHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA