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The map label for this gene is prfC [H]

Identifier: 57650211

GI number: 57650211

Start: 1033818

End: 1035380

Strand: Direct

Name: prfC [H]

Synonym: SACOL1025

Alternate gene names: 57650211

Gene position: 1033818-1035380 (Clockwise)

Preceding gene: 57650210

Following gene: 57650212

Centisome position: 36.8

GC content: 34.04

Gene sequence:

>1563_bases
ATGAACTTAAAGCAAGAAGTTGAGTCTAGAAAGACTTTTGCGATTATTTCACATCCCGATGCAGGGAAAACAACGTTAAC
TGAAAAACTATTGTACTTCAGTGGTGCTATTCGTGAAGCGGGTACAGTTAAAGGGAAGAAGACTGGTAAATTTGCGACAA
GTGACTGGATGAAAGTTGAACAAGAGCGTGGTATTTCTGTAACTAGTTCAGTAATGCAATTTGATTACGATGATTATAAA
ATCAATATCTTAGATACACCAGGACATGAAGACTTTTCAGAAGATACGTATAGAACATTAATGGCAGTTGACAGTGCTGT
CATGGTCATAGACTGTGCAAAAGGTATTGAACCACAAACATTGAAGTTATTTAAAGTTTGTAAAATGCGTGGTATTCCAA
TCTTTACATTCATTAATAAATTAGACCGAGTAGGTAAAGAACCATTTGAATTATTAGATGAAATCGAAGAGACATTAAAT
ATTGAAACATACCCTATGAATTGGCCAATTGGTATGGGACAAAGTTTCTTTGGCATCATTGATAGAAAGTCTAAAACAAT
TGAACCATTTAGAGATGAAGAAAATATATTACATTTGAATGATGATTTTGAGTTGGAAGAAGATCATGCAATTACAAATG
ATAGTGATTTTGAACAAGCGATTGAAGAATTAATGTTGGTTGAAGAAGCGGGTGAAGCCTTTGATAATGACGCGCTGTTG
AGTGGAGACTTAACACCTGTATTTTTCGGTTCAGCTTTAGCTAACTTTGGTGTACAAAATTTCTTAAATGCATATGTTGA
TTTTGCGCCAATGCCAAATGCGAGACAAACAAAAGAAGACGTTGAAGTAAGCCCGTTTGATGATTCATTTTCAGGATTTA
TCTTTAAAATTCAAGCCAACATGGACCCTAAACACCGTGATAGAATTGCCTTTATGCGTGTCGTTAGTGGTGCATTTGAA
CGTGGTATGGATGTTACTTTGCAACGTACTAATAAAAAGCAAAAGATCACACGTTCAACGTCATTTATGGCAGACGATAA
AGAAACTGTGAATCATGCTGTAGCAGGCGATATCATTGGACTATATGATACTGGTAATTATCAAATTGGAGATACTTTAG
TTGGTGGAAAACAAACCTACAGTTTCCAAGATTTACCACAATTTACGCCAGAAATTTTTATGAAAGTTTCTGCTAAAAAC
GTCATGAAACAGAAGCATTTCCATAAAGGTATTGAACAATTAGTACAAGAAGGTGCGATTCAATACTATAAAACATTACA
CACAAACCAAATTATTTTAGGTGCTGTTGGTCAGTTACAATTTGAAGTTTTCGAACATAGAATGAAAAACGAATATAATG
TTGATGTTGTTATGGAGCCAGTAGGCCGTAAAATTGCACGTTGGATTGAAAATGAAGACCAAATTACAGATAAGATGAAC
ACATCAAGATCGATTTTAGTGAAAGATAGATATGACGATTTAGTATTCTTATTTGAAAATGAATTTGCAACAAGATGGTT
TGAAGAGAAATTCCCTGAAATTAAATTGTATAGTTTACTTTAA

Upstream 100 bases:

>100_bases
TACTCATGCAATTGTTTGTTTTTAAAGAAGAGGAAGAGGCAGAATCGATTGCATCACAACTAACAGATTGGATAGAAACA
TATAAAAAGGAGAAAGACTA

Downstream 100 bases:

>100_bases
CAGCTCAATTGTATAATCGAATTTGTTACATTAAAAATAATTGTTTCGTTGAAGAAAAATAAATTGTATATTTTAAAAGA
AAAAGGTATACTATGATGTA

Product: peptide chain release factor 3

Products: NA

Alternate protein names: RF-3 [H]

Number of amino acids: Translated: 520; Mature: 520

Protein sequence:

>520_residues
MNLKQEVESRKTFAIISHPDAGKTTLTEKLLYFSGAIREAGTVKGKKTGKFATSDWMKVEQERGISVTSSVMQFDYDDYK
INILDTPGHEDFSEDTYRTLMAVDSAVMVIDCAKGIEPQTLKLFKVCKMRGIPIFTFINKLDRVGKEPFELLDEIEETLN
IETYPMNWPIGMGQSFFGIIDRKSKTIEPFRDEENILHLNDDFELEEDHAITNDSDFEQAIEELMLVEEAGEAFDNDALL
SGDLTPVFFGSALANFGVQNFLNAYVDFAPMPNARQTKEDVEVSPFDDSFSGFIFKIQANMDPKHRDRIAFMRVVSGAFE
RGMDVTLQRTNKKQKITRSTSFMADDKETVNHAVAGDIIGLYDTGNYQIGDTLVGGKQTYSFQDLPQFTPEIFMKVSAKN
VMKQKHFHKGIEQLVQEGAIQYYKTLHTNQIILGAVGQLQFEVFEHRMKNEYNVDVVMEPVGRKIARWIENEDQITDKMN
TSRSILVKDRYDDLVFLFENEFATRWFEEKFPEIKLYSLL

Sequences:

>Translated_520_residues
MNLKQEVESRKTFAIISHPDAGKTTLTEKLLYFSGAIREAGTVKGKKTGKFATSDWMKVEQERGISVTSSVMQFDYDDYK
INILDTPGHEDFSEDTYRTLMAVDSAVMVIDCAKGIEPQTLKLFKVCKMRGIPIFTFINKLDRVGKEPFELLDEIEETLN
IETYPMNWPIGMGQSFFGIIDRKSKTIEPFRDEENILHLNDDFELEEDHAITNDSDFEQAIEELMLVEEAGEAFDNDALL
SGDLTPVFFGSALANFGVQNFLNAYVDFAPMPNARQTKEDVEVSPFDDSFSGFIFKIQANMDPKHRDRIAFMRVVSGAFE
RGMDVTLQRTNKKQKITRSTSFMADDKETVNHAVAGDIIGLYDTGNYQIGDTLVGGKQTYSFQDLPQFTPEIFMKVSAKN
VMKQKHFHKGIEQLVQEGAIQYYKTLHTNQIILGAVGQLQFEVFEHRMKNEYNVDVVMEPVGRKIARWIENEDQITDKMN
TSRSILVKDRYDDLVFLFENEFATRWFEEKFPEIKLYSLL
>Mature_520_residues
MNLKQEVESRKTFAIISHPDAGKTTLTEKLLYFSGAIREAGTVKGKKTGKFATSDWMKVEQERGISVTSSVMQFDYDDYK
INILDTPGHEDFSEDTYRTLMAVDSAVMVIDCAKGIEPQTLKLFKVCKMRGIPIFTFINKLDRVGKEPFELLDEIEETLN
IETYPMNWPIGMGQSFFGIIDRKSKTIEPFRDEENILHLNDDFELEEDHAITNDSDFEQAIEELMLVEEAGEAFDNDALL
SGDLTPVFFGSALANFGVQNFLNAYVDFAPMPNARQTKEDVEVSPFDDSFSGFIFKIQANMDPKHRDRIAFMRVVSGAFE
RGMDVTLQRTNKKQKITRSTSFMADDKETVNHAVAGDIIGLYDTGNYQIGDTLVGGKQTYSFQDLPQFTPEIFMKVSAKN
VMKQKHFHKGIEQLVQEGAIQYYKTLHTNQIILGAVGQLQFEVFEHRMKNEYNVDVVMEPVGRKIARWIENEDQITDKMN
TSRSILVKDRYDDLVFLFENEFATRWFEEKFPEIKLYSLL

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=492, Percent_Identity=30.8943089430894, Blast_Score=199, Evalue=6e-51,
Organism=Homo sapiens, GI19923640, Length=525, Percent_Identity=26.6666666666667, Blast_Score=177, Evalue=2e-44,
Organism=Homo sapiens, GI25306283, Length=392, Percent_Identity=30.8673469387755, Blast_Score=165, Evalue=1e-40,
Organism=Homo sapiens, GI25306287, Length=496, Percent_Identity=25.8064516129032, Blast_Score=149, Evalue=7e-36,
Organism=Homo sapiens, GI157426893, Length=161, Percent_Identity=32.2981366459627, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI94966754, Length=174, Percent_Identity=29.3103448275862, Blast_Score=85, Evalue=2e-16,
Organism=Homo sapiens, GI4503483, Length=149, Percent_Identity=29.5302013422819, Blast_Score=77, Evalue=5e-14,
Organism=Homo sapiens, GI310132016, Length=123, Percent_Identity=31.7073170731707, Blast_Score=74, Evalue=4e-13,
Organism=Homo sapiens, GI310110807, Length=123, Percent_Identity=31.7073170731707, Blast_Score=74, Evalue=4e-13,
Organism=Homo sapiens, GI310123363, Length=123, Percent_Identity=31.7073170731707, Blast_Score=74, Evalue=4e-13,
Organism=Homo sapiens, GI84043963, Length=135, Percent_Identity=31.8518518518519, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI1790835, Length=527, Percent_Identity=47.6280834914611, Blast_Score=483, Evalue=1e-137,
Organism=Escherichia coli, GI1789738, Length=479, Percent_Identity=28.39248434238, Blast_Score=184, Evalue=1e-47,
Organism=Escherichia coli, GI48994988, Length=176, Percent_Identity=33.5227272727273, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1788922, Length=158, Percent_Identity=30.379746835443, Blast_Score=95, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17533571, Length=485, Percent_Identity=28.4536082474227, Blast_Score=192, Evalue=3e-49,
Organism=Caenorhabditis elegans, GI17556745, Length=177, Percent_Identity=35.5932203389831, Blast_Score=126, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI17557151, Length=137, Percent_Identity=32.8467153284672, Blast_Score=90, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17506493, Length=186, Percent_Identity=29.0322580645161, Blast_Score=83, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI71988819, Length=140, Percent_Identity=31.4285714285714, Blast_Score=75, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI71988811, Length=140, Percent_Identity=31.4285714285714, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17552884, Length=112, Percent_Identity=34.8214285714286, Blast_Score=69, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17569207, Length=112, Percent_Identity=34.8214285714286, Blast_Score=69, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI32566301, Length=113, Percent_Identity=35.3982300884956, Blast_Score=69, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI32566303, Length=111, Percent_Identity=35.1351351351351, Blast_Score=68, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17556456, Length=170, Percent_Identity=29.4117647058824, Blast_Score=67, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6323098, Length=478, Percent_Identity=28.8702928870293, Blast_Score=193, Evalue=6e-50,
Organism=Saccharomyces cerevisiae, GI6322359, Length=505, Percent_Identity=27.3267326732673, Blast_Score=147, Evalue=5e-36,
Organism=Saccharomyces cerevisiae, GI6324707, Length=549, Percent_Identity=22.7686703096539, Blast_Score=86, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6320593, Length=549, Percent_Identity=22.7686703096539, Blast_Score=86, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6323320, Length=142, Percent_Identity=30.2816901408451, Blast_Score=81, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6325337, Length=118, Percent_Identity=31.3559322033898, Blast_Score=65, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6319594, Length=118, Percent_Identity=31.3559322033898, Blast_Score=65, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6322937, Length=151, Percent_Identity=29.8013245033113, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24582462, Length=482, Percent_Identity=29.8755186721992, Blast_Score=210, Evalue=2e-54,
Organism=Drosophila melanogaster, GI221458488, Length=502, Percent_Identity=26.2948207171315, Blast_Score=163, Evalue=3e-40,
Organism=Drosophila melanogaster, GI78706572, Length=190, Percent_Identity=32.1052631578947, Blast_Score=103, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24585711, Length=210, Percent_Identity=28.0952380952381, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24585713, Length=210, Percent_Identity=28.0952380952381, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24585709, Length=178, Percent_Identity=29.2134831460674, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI28574573, Length=151, Percent_Identity=31.7880794701987, Blast_Score=71, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: NA

Molecular weight: Translated: 59602; Mature: 59602

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLKQEVESRKTFAIISHPDAGKTTLTEKLLYFSGAIREAGTVKGKKTGKFATSDWMKVE
CCCHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHH
QERGISVTSSVMQFDYDDYKINILDTPGHEDFSEDTYRTLMAVDSAVMVIDCAKGIEPQT
HHCCCHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCHHH
LKLFKVCKMRGIPIFTFINKLDRVGKEPFELLDEIEETLNIETYPMNWPIGMGQSFFGII
HHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEECCCCCCCCCCHHHHHH
DRKSKTIEPFRDEENILHLNDDFELEEDHAITNDSDFEQAIEELMLVEEAGEAFDNDALL
CCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEE
SGDLTPVFFGSALANFGVQNFLNAYVDFAPMPNARQTKEDVEVSPFDDSFSGFIFKIQAN
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHCCCCCCCCCCCCCEEEEEECC
MDPKHRDRIAFMRVVSGAFERGMDVTLQRTNKKQKITRSTSFMADDKETVNHAVAGDIIG
CCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHCCEEE
LYDTGNYQIGDTLVGGKQTYSFQDLPQFTPEIFMKVSAKNVMKQKHFHKGIEQLVQEGAI
EEECCCEEECCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QYYKTLHTNQIILGAVGQLQFEVFEHRMKNEYNVDVVMEPVGRKIARWIENEDQITDKMN
HHHHHHCCCEEEEEHHHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHCCHHHHHHHCC
TSRSILVKDRYDDLVFLFENEFATRWFEEKFPEIKLYSLL
CCCEEEEEECCCCEEEEEECHHHHHHHHHCCCCEEEEECC
>Mature Secondary Structure
MNLKQEVESRKTFAIISHPDAGKTTLTEKLLYFSGAIREAGTVKGKKTGKFATSDWMKVE
CCCHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHH
QERGISVTSSVMQFDYDDYKINILDTPGHEDFSEDTYRTLMAVDSAVMVIDCAKGIEPQT
HHCCCHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCHHH
LKLFKVCKMRGIPIFTFINKLDRVGKEPFELLDEIEETLNIETYPMNWPIGMGQSFFGII
HHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEECCCCCCCCCCHHHHHH
DRKSKTIEPFRDEENILHLNDDFELEEDHAITNDSDFEQAIEELMLVEEAGEAFDNDALL
CCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEE
SGDLTPVFFGSALANFGVQNFLNAYVDFAPMPNARQTKEDVEVSPFDDSFSGFIFKIQAN
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHCCCCCCCCCCCCCEEEEEECC
MDPKHRDRIAFMRVVSGAFERGMDVTLQRTNKKQKITRSTSFMADDKETVNHAVAGDIIG
CCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHCCEEE
LYDTGNYQIGDTLVGGKQTYSFQDLPQFTPEIFMKVSAKNVMKQKHFHKGIEQLVQEGAI
EEECCCEEECCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QYYKTLHTNQIILGAVGQLQFEVFEHRMKNEYNVDVVMEPVGRKIARWIENEDQITDKMN
HHHHHHCCCEEEEEHHHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHCCHHHHHHHCC
TSRSILVKDRYDDLVFLFENEFATRWFEEKFPEIKLYSLL
CCCEEEEEECCCCEEEEEECHHHHHHHHHCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA