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The map label for this gene is tuf

Identifier: 41410241

GI number: 41410241

Start: 4620946

End: 4622136

Strand: Direct

Name: tuf

Synonym: MAP4143

Alternate gene names: 41410241

Gene position: 4620946-4622136 (Clockwise)

Preceding gene: 41410240

Following gene: 41410242

Centisome position: 95.68

GC content: 65.99

Gene sequence:

>1191_bases
GTGGCGAAGGCGAAGTTCGAGCGGACGAAGCCGCACGTCAACATCGGGACCATCGGTCACGTTGACCACGGCAAGACCAC
GCTGACCGCGGCTATCACCAAGGTTCTGCACGACAAGTACCCGGACCTGAACGAGTCCCGCGCGTTCGACCAGATCGACA
ACGCGCCCGAGGAGCGTCAGCGCGGTATCACCATCAACATCTCCCACGTGGAGTACCAGACCGACAAGCGGCACTACGCT
CACGTCGACGCCCCGGGTCACGCCGACTACATCAAGAACATGATCACCGGCGCCGCCCAGATGGACGGCGCGATCCTGGT
GGTCGCCGCCACCGACGGCCCGATGCCGCAGACCCGCGAGCACGTGCTGCTGGCCCGTCAGGTCGGTGTGCCCTACATCC
TGGTCGCGCTGAACAAGGCCGACATGGTCGACGACGAGGAGCTGCTCGAGCTCGTCGAGATGGAGGTCCGCGAGCTGCTG
GCCGCCCAGGAGTTCGACGAGGACGCCCCGGTGGTGCGGGTGTCGGCGCTCAAGGCGCTCGAGGGCGACGCCAAGTGGGT
GGAGTCCGTCGAGCAGCTGATGGAGGCCGTCGACGAGTCGATCCCGGACCCGGTCCGCGAGACGGACAAGCCGTTCCTGA
TGCCGGTGGAGGACGTCTTCACCATCACCGGTCGTGGCACGGTGGTCACCGGCCGTGTCGAGCGCGGCGTGATCAACGTG
AACGAGGAAGTCGAGATCGTCGGCATCCGCCCGTCCAGCACCAAGACCACGGTCACCGGTGTGGAGATGTTCCGCAAGCT
GCTCGACCAGGGCCAGGCCGGTGACAACGTCGGTCTGCTGCTGCGTGGTATCAAGCGTGAGGACGTCGAGCGCGGTCAGG
TCGTCACCAAGCCCGGCACCACCACGCCGCACACCGAGTTCGAGGGCCAGGTCTACATCCTGTCCAAGGACGAGGGCGGC
CGGCACACGCCGTTCTTCAACAACTACCGCCCGCAGTTCTACTTCCGCACCACCGACGTGACCGGTGTGGTGACGCTGCC
GGAGGGCACCGAGATGGTGATGCCCGGTGACAACACCAACATCTCGGTGAAGCTGATCCAGCCCGTCGCCATGGACGAGG
GTCTGCGGTTCGCCATCCGCGAGGGTGGTCGCACCGTCGGCGCCGGCCGGGTCGTCAAGATCATCAAGTAG

Upstream 100 bases:

>100_bases
GGAGTAATCTTGCCGGGTCATTGGAATGCCTTGGGCGCGGCACAACTGAAAACACCAACACTGCTTTAACAAGCACCAAC
TAGTCCAGGAGGACACAGAA

Downstream 100 bases:

>100_bases
TTCGCTCAGTCGGTTCAAAGCCCGGATTGCCCACGCGGCGATCCGGGCTTTGTTCTGCTGTGCGCCCCGCGGACCCGGAT
CGACGCCGGAGAAATAATGC

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu [H]

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MAKAKFERTKPHVNIGTIGHVDHGKTTLTAAITKVLHDKYPDLNESRAFDQIDNAPEERQRGITINISHVEYQTDKRHYA
HVDAPGHADYIKNMITGAAQMDGAILVVAATDGPMPQTREHVLLARQVGVPYILVALNKADMVDDEELLELVEMEVRELL
AAQEFDEDAPVVRVSALKALEGDAKWVESVEQLMEAVDESIPDPVRETDKPFLMPVEDVFTITGRGTVVTGRVERGVINV
NEEVEIVGIRPSSTKTTVTGVEMFRKLLDQGQAGDNVGLLLRGIKREDVERGQVVTKPGTTTPHTEFEGQVYILSKDEGG
RHTPFFNNYRPQFYFRTTDVTGVVTLPEGTEMVMPGDNTNISVKLIQPVAMDEGLRFAIREGGRTVGAGRVVKIIK

Sequences:

>Translated_396_residues
MAKAKFERTKPHVNIGTIGHVDHGKTTLTAAITKVLHDKYPDLNESRAFDQIDNAPEERQRGITINISHVEYQTDKRHYA
HVDAPGHADYIKNMITGAAQMDGAILVVAATDGPMPQTREHVLLARQVGVPYILVALNKADMVDDEELLELVEMEVRELL
AAQEFDEDAPVVRVSALKALEGDAKWVESVEQLMEAVDESIPDPVRETDKPFLMPVEDVFTITGRGTVVTGRVERGVINV
NEEVEIVGIRPSSTKTTVTGVEMFRKLLDQGQAGDNVGLLLRGIKREDVERGQVVTKPGTTTPHTEFEGQVYILSKDEGG
RHTPFFNNYRPQFYFRTTDVTGVVTLPEGTEMVMPGDNTNISVKLIQPVAMDEGLRFAIREGGRTVGAGRVVKIIK
>Mature_395_residues
AKAKFERTKPHVNIGTIGHVDHGKTTLTAAITKVLHDKYPDLNESRAFDQIDNAPEERQRGITINISHVEYQTDKRHYAH
VDAPGHADYIKNMITGAAQMDGAILVVAATDGPMPQTREHVLLARQVGVPYILVALNKADMVDDEELLELVEMEVRELLA
AQEFDEDAPVVRVSALKALEGDAKWVESVEQLMEAVDESIPDPVRETDKPFLMPVEDVFTITGRGTVVTGRVERGVINVN
EEVEIVGIRPSSTKTTVTGVEMFRKLLDQGQAGDNVGLLLRGIKREDVERGQVVTKPGTTTPHTEFEGQVYILSKDEGGR
HTPFFNNYRPQFYFRTTDVTGVVTLPEGTEMVMPGDNTNISVKLIQPVAMDEGLRFAIREGGRTVGAGRVVKIIK

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=397, Percent_Identity=54.6599496221663, Blast_Score=442, Evalue=1e-124,
Organism=Homo sapiens, GI4503471, Length=448, Percent_Identity=31.0267857142857, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI4503475, Length=358, Percent_Identity=33.5195530726257, Blast_Score=163, Evalue=2e-40,
Organism=Homo sapiens, GI223555963, Length=441, Percent_Identity=31.0657596371882, Blast_Score=155, Evalue=4e-38,
Organism=Homo sapiens, GI5729864, Length=441, Percent_Identity=31.0657596371882, Blast_Score=155, Evalue=7e-38,
Organism=Homo sapiens, GI194018522, Length=337, Percent_Identity=28.486646884273, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI194018520, Length=337, Percent_Identity=28.486646884273, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI194097354, Length=337, Percent_Identity=28.486646884273, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI54607086, Length=325, Percent_Identity=27.6923076923077, Blast_Score=115, Evalue=6e-26,
Organism=Homo sapiens, GI46094014, Length=338, Percent_Identity=28.698224852071, Blast_Score=113, Evalue=3e-25,
Organism=Homo sapiens, GI4503507, Length=307, Percent_Identity=26.7100977198697, Blast_Score=90, Evalue=3e-18,
Organism=Homo sapiens, GI82546879, Length=439, Percent_Identity=21.8678815489749, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI157426893, Length=235, Percent_Identity=25.531914893617, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI1790412, Length=396, Percent_Identity=75.2525252525252, Blast_Score=581, Evalue=1e-167,
Organism=Escherichia coli, GI1789737, Length=396, Percent_Identity=75.2525252525252, Blast_Score=581, Evalue=1e-167,
Organism=Escherichia coli, GI2367247, Length=339, Percent_Identity=31.2684365781711, Blast_Score=132, Evalue=5e-32,
Organism=Escherichia coli, GI1789108, Length=328, Percent_Identity=28.9634146341463, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI48994988, Length=242, Percent_Identity=33.0578512396694, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI1788922, Length=330, Percent_Identity=24.8484848484848, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17556456, Length=400, Percent_Identity=49.75, Blast_Score=363, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI25141371, Length=374, Percent_Identity=44.3850267379679, Blast_Score=317, Evalue=7e-87,
Organism=Caenorhabditis elegans, GI17552884, Length=352, Percent_Identity=34.0909090909091, Blast_Score=165, Evalue=4e-41,
Organism=Caenorhabditis elegans, GI17569207, Length=352, Percent_Identity=34.0909090909091, Blast_Score=165, Evalue=4e-41,
Organism=Caenorhabditis elegans, GI32566303, Length=342, Percent_Identity=32.4561403508772, Blast_Score=142, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI115532067, Length=432, Percent_Identity=28.2407407407407, Blast_Score=139, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI115532065, Length=432, Percent_Identity=28.2407407407407, Blast_Score=138, Evalue=5e-33,
Organism=Caenorhabditis elegans, GI17506081, Length=274, Percent_Identity=31.3868613138686, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI32566629, Length=450, Percent_Identity=24.4444444444444, Blast_Score=119, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI17509919, Length=296, Percent_Identity=27.027027027027, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI32566301, Length=150, Percent_Identity=41.3333333333333, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17557151, Length=232, Percent_Identity=26.2931034482759, Blast_Score=77, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6324761, Length=404, Percent_Identity=61.6336633663366, Blast_Score=486, Evalue=1e-138,
Organism=Saccharomyces cerevisiae, GI6325337, Length=349, Percent_Identity=34.9570200573066, Blast_Score=167, Evalue=3e-42,
Organism=Saccharomyces cerevisiae, GI6319594, Length=349, Percent_Identity=34.9570200573066, Blast_Score=167, Evalue=3e-42,
Organism=Saccharomyces cerevisiae, GI6322937, Length=353, Percent_Identity=27.7620396600567, Blast_Score=106, Evalue=7e-24,
Organism=Saccharomyces cerevisiae, GI6320377, Length=343, Percent_Identity=28.5714285714286, Blast_Score=97, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6320863, Length=281, Percent_Identity=25.6227758007117, Blast_Score=83, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6323320, Length=237, Percent_Identity=28.6919831223629, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI281363316, Length=396, Percent_Identity=58.3333333333333, Blast_Score=465, Evalue=1e-131,
Organism=Drosophila melanogaster, GI17864358, Length=396, Percent_Identity=58.3333333333333, Blast_Score=465, Evalue=1e-131,
Organism=Drosophila melanogaster, GI19921738, Length=389, Percent_Identity=50.6426735218509, Blast_Score=386, Evalue=1e-107,
Organism=Drosophila melanogaster, GI45553807, Length=441, Percent_Identity=30.8390022675737, Blast_Score=160, Evalue=1e-39,
Organism=Drosophila melanogaster, GI45553816, Length=441, Percent_Identity=30.8390022675737, Blast_Score=160, Evalue=1e-39,
Organism=Drosophila melanogaster, GI24651721, Length=441, Percent_Identity=30.8390022675737, Blast_Score=160, Evalue=1e-39,
Organism=Drosophila melanogaster, GI17864154, Length=441, Percent_Identity=30.8390022675737, Blast_Score=160, Evalue=1e-39,
Organism=Drosophila melanogaster, GI24652838, Length=448, Percent_Identity=29.6875, Blast_Score=159, Evalue=5e-39,
Organism=Drosophila melanogaster, GI17137572, Length=448, Percent_Identity=29.6875, Blast_Score=159, Evalue=5e-39,
Organism=Drosophila melanogaster, GI45550900, Length=340, Percent_Identity=31.1764705882353, Blast_Score=135, Evalue=5e-32,
Organism=Drosophila melanogaster, GI19922690, Length=305, Percent_Identity=30.4918032786885, Blast_Score=135, Evalue=6e-32,
Organism=Drosophila melanogaster, GI17137380, Length=338, Percent_Identity=30.1775147928994, Blast_Score=125, Evalue=6e-29,
Organism=Drosophila melanogaster, GI24647046, Length=304, Percent_Identity=25.3289473684211, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24647048, Length=304, Percent_Identity=25.3289473684211, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI28572034, Length=188, Percent_Identity=36.1702127659575, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI78706572, Length=249, Percent_Identity=29.3172690763052, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 43771; Mature: 43640

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKAKFERTKPHVNIGTIGHVDHGKTTLTAAITKVLHDKYPDLNESRAFDQIDNAPEERQ
CCCCCCCCCCCCEEECEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCHHHHH
RGITINISHVEYQTDKRHYAHVDAPGHADYIKNMITGAAQMDGAILVVAATDGPMPQTRE
CCEEEEEEEEEEECCCCEEEEECCCCHHHHHHHHHHCHHHCCCEEEEEEECCCCCCCCHH
HVLLARQVGVPYILVALNKADMVDDEELLELVEMEVRELLAAQEFDEDAPVVRVSALKAL
HHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHH
EGDAKWVESVEQLMEAVDESIPDPVRETDKPFLMPVEDVFTITGRGTVVTGRVERGVINV
CCCHHHHHHHHHHHHHHHHHCCCCHHHCCCCEEEEHHHEEEEECCCEEEEEEEECCEEEC
NEEVEIVGIRPSSTKTTVTGVEMFRKLLDQGQAGDNVGLLLRGIKREDVERGQVVTKPGT
CCCEEEEEECCCCCCEEEHHHHHHHHHHHCCCCCCCHHHEEECCCHHCCCCCEEEECCCC
TTPHTEFEGQVYILSKDEGGRHTPFFNNYRPQFYFRTTDVTGVVTLPEGTEMVMPGDNTN
CCCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEECCCEEEEECCCCCEEEECCCCCE
ISVKLIQPVAMDEGLRFAIREGGRTVGAGRVVKIIK
EEEEEECCCCCCCCCEEEECCCCCEECCCCEEEEEC
>Mature Secondary Structure 
AKAKFERTKPHVNIGTIGHVDHGKTTLTAAITKVLHDKYPDLNESRAFDQIDNAPEERQ
CCCCCCCCCCCEEECEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCHHHHH
RGITINISHVEYQTDKRHYAHVDAPGHADYIKNMITGAAQMDGAILVVAATDGPMPQTRE
CCEEEEEEEEEEECCCCEEEEECCCCHHHHHHHHHHCHHHCCCEEEEEEECCCCCCCCHH
HVLLARQVGVPYILVALNKADMVDDEELLELVEMEVRELLAAQEFDEDAPVVRVSALKAL
HHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHH
EGDAKWVESVEQLMEAVDESIPDPVRETDKPFLMPVEDVFTITGRGTVVTGRVERGVINV
CCCHHHHHHHHHHHHHHHHHCCCCHHHCCCCEEEEHHHEEEEECCCEEEEEEEECCEEEC
NEEVEIVGIRPSSTKTTVTGVEMFRKLLDQGQAGDNVGLLLRGIKREDVERGQVVTKPGT
CCCEEEEEECCCCCCEEEHHHHHHHHHHHCCCCCCCHHHEEECCCHHCCCCCEEEECCCC
TTPHTEFEGQVYILSKDEGGRHTPFFNNYRPQFYFRTTDVTGVVTLPEGTEMVMPGDNTN
CCCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEECCCEEEEECCCCCEEEECCCCCE
ISVKLIQPVAMDEGLRFAIREGGRTVGAGRVVKIIK
EEEEEECCCCCCCCCEEEECCCCCEECCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA