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The map label for this gene is thiG

Identifier: 41409998

GI number: 41409998

Start: 4364303

End: 4365061

Strand: Direct

Name: thiG

Synonym: MAP3900

Alternate gene names: 41409998

Gene position: 4364303-4365061 (Clockwise)

Preceding gene: 41409997

Following gene: 41409999

Centisome position: 90.36

GC content: 70.75

Gene sequence:

>759_bases
GTGGTTGATTCGAAACTGACCATCGCCGACCGCAGCTTCAACTCGCGGCTCATCATGGGCACCGGGGGAGCGGGCAACCT
GGCGGTGCTGGAAGAGGCGCTGATCGCCTCGGGCACCGAACTGACCACCGTCGCCATCCGCCGGGTGGACGCCGAGGGCG
GCACCGGCCTGCTCGACCTGCTCAACCGGCTGGGCATCACACCGTTGCCCAACACCGCGGGATGCCGGGGCGCGGCCGAG
GCGGTGCTCACCGCGCAGTTGGCCCGCGAGGCGCTGAACACCAACTGGATCAAGCTCGAGGTGATCGCCGACGAGCGCAC
CCTGCTGCCCGATGCGGTCGAATTGGTCAGGGCCGCAGAACAATTGGTGGACGACGGGTTCGTCGTGCTGCCCTACACCA
ACGACGACCCGGTGTTGGCGCGCCGACTCGAGGACGCCGGCTGCGCGGCGGTCATGCCGCTGGGCTCGCCGATCGGCACG
GGTCTGGGCATCACCAATCCGCACAACATCGAGATGATCGTCGCCGGCGCGGGCGTCCCGGTGGTGCTCGACGCCGGCAT
CGGCACCGCCAGCGATGCCGCGCTGGCGATGGAATTGGGTTGCGACGCCGTGCTGTTGGCCACCGCGGTGACCCGGGCCG
GCGATCCGGCCGCCATGGCCGCCGCGATGTCGGCCGCCGTCACCGCCGGCTATCTGGCCCGCCGGGCCGGGCGGATCCCC
AAGCGGTTCTGGGCCCAAGCGTCGAGCCCGCCTCGATGA

Upstream 100 bases:

>100_bases
GGCGTCGCGGTGGCCGTGGACGACGCCGTGTTGCCCCGATCCCGTTGGACGACAGAGCTTTCCGAGGGCGCGCGGCTCGA
GGTGGTGACGGCGGTGCAGG

Downstream 100 bases:

>100_bases
AACGCTATGTGGCGCTGGGCAGTTCGATGGCCGCCGGCCCCGGGATCCGGCCCCGCGCCGCCGGGGCTCCGCGCTGGTCG
GGTCGATCCGCGCGCAACTA

Product: thiazole synthase

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MVDSKLTIADRSFNSRLIMGTGGAGNLAVLEEALIASGTELTTVAIRRVDAEGGTGLLDLLNRLGITPLPNTAGCRGAAE
AVLTAQLAREALNTNWIKLEVIADERTLLPDAVELVRAAEQLVDDGFVVLPYTNDDPVLARRLEDAGCAAVMPLGSPIGT
GLGITNPHNIEMIVAGAGVPVVLDAGIGTASDAALAMELGCDAVLLATAVTRAGDPAAMAAAMSAAVTAGYLARRAGRIP
KRFWAQASSPPR

Sequences:

>Translated_252_residues
MVDSKLTIADRSFNSRLIMGTGGAGNLAVLEEALIASGTELTTVAIRRVDAEGGTGLLDLLNRLGITPLPNTAGCRGAAE
AVLTAQLAREALNTNWIKLEVIADERTLLPDAVELVRAAEQLVDDGFVVLPYTNDDPVLARRLEDAGCAAVMPLGSPIGT
GLGITNPHNIEMIVAGAGVPVVLDAGIGTASDAALAMELGCDAVLLATAVTRAGDPAAMAAAMSAAVTAGYLARRAGRIP
KRFWAQASSPPR
>Mature_252_residues
MVDSKLTIADRSFNSRLIMGTGGAGNLAVLEEALIASGTELTTVAIRRVDAEGGTGLLDLLNRLGITPLPNTAGCRGAAE
AVLTAQLAREALNTNWIKLEVIADERTLLPDAVELVRAAEQLVDDGFVVLPYTNDDPVLARRLEDAGCAAVMPLGSPIGT
GLGITNPHNIEMIVAGAGVPVVLDAGIGTASDAALAMELGCDAVLLATAVTRAGDPAAMAAAMSAAVTAGYLARRAGRIP
KRFWAQASSPPR

Specific function: Catalyzes the rearrangement of 1-deoxy-D-xylulose 5- phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S [H]

COG id: COG2022

COG function: function code H; Uncharacterized enzyme of thiazole biosynthesis

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiG family [H]

Homologues:

Organism=Escherichia coli, GI48994993, Length=246, Percent_Identity=51.6260162601626, Blast_Score=240, Evalue=7e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR008867 [H]

Pfam domain/function: PF05690 ThiG [H]

EC number: NA

Molecular weight: Translated: 25832; Mature: 25832

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVDSKLTIADRSFNSRLIMGTGGAGNLAVLEEALIASGTELTTVAIRRVDAEGGTGLLDL
CCCCEEEEECCCCCCEEEEECCCCCCHHHHHHHHHHCCCCEEEEEEEEECCCCCCCHHHH
LNRLGITPLPNTAGCRGAAEAVLTAQLAREALNTNWIKLEVIADERTLLPDAVELVRAAE
HHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHH
QLVDDGFVVLPYTNDDPVLARRLEDAGCAAVMPLGSPIGTGLGITNPHNIEMIVAGAGVP
HHHHCCEEEEEECCCCHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCEEEEEECCCCC
VVLDAGIGTASDAALAMELGCDAVLLATAVTRAGDPAAMAAAMSAAVTAGYLARRAGRIP
EEEECCCCCCCCHHEEHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
KRFWAQASSPPR
HHHHHCCCCCCC
>Mature Secondary Structure
MVDSKLTIADRSFNSRLIMGTGGAGNLAVLEEALIASGTELTTVAIRRVDAEGGTGLLDL
CCCCEEEEECCCCCCEEEEECCCCCCHHHHHHHHHHCCCCEEEEEEEEECCCCCCCHHHH
LNRLGITPLPNTAGCRGAAEAVLTAQLAREALNTNWIKLEVIADERTLLPDAVELVRAAE
HHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHH
QLVDDGFVVLPYTNDDPVLARRLEDAGCAAVMPLGSPIGTGLGITNPHNIEMIVAGAGVP
HHHHCCEEEEEECCCCHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCEEEEEECCCCC
VVLDAGIGTASDAALAMELGCDAVLLATAVTRAGDPAAMAAAMSAAVTAGYLARRAGRIP
EEEECCCCCCCCHHEEHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
KRFWAQASSPPR
HHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA