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The map label for this gene is purT

Identifier: 41409969

GI number: 41409969

Start: 4332408

End: 4333670

Strand: Direct

Name: purT

Synonym: MAP3871

Alternate gene names: 41409969

Gene position: 4332408-4333670 (Clockwise)

Preceding gene: 41409968

Following gene: 41409970

Centisome position: 89.7

GC content: 76.96

Gene sequence:

>1263_bases
GTGACTGACGGCGTGACCGACGGACGGGACGACGAGACGACGGCGCTCGCCGTGCCCAGGCCCGCTCCCGCCGACCGCGG
CCCGGCTCCCGCCGACGGCAGCCCGGCTCCCGCCGACGGCAGGCCGGCGGTGATGCTGCTGGGCTCCGGCGACATCAGCC
GCGAGCTGGCGATCGCGCTGGGGCGGCTGGGCGCGCGGGTGATCGCCGTCGACGCGCACCCGCAGGCGCCCGCGCACGCG
GTGGCCGACCAGGCGCTGGTGCTGCCGTCGACCGACGCCGGCGAGCTGTCGGCGGCGATTCACCGGCTGCGACCCGACGT
CGTGGTAAGTACCACCGACGCCGTCGCGGTGCCGGCCCTCGAGGCGCTGGACGGCACGGGCGCCGAGCCCGTGCCCAGCG
CCCGCGCCGTGCGGCTGGCCGCCGACCGGGAAGGGCTGCGCCGGCTGGCCGCCGACGAGCTGGGGCTGCCCACCGCGCCG
TTCTGGTTCGCCGGATCGCTGGGTGAACTCGAGGCGGTGGGCGCGCACGCCGGATACCCGTTGCTGGTCAAGCCGGTGGT
CGGGGCGGTCGGGCCGCGGCAGTCGGTGGTGGCGGGCCGTGAGGACATCGCGGCCGCCTGGCACCGCGCGGTGGACGGTC
AGCCCGGCGCCCGGGTGCTGGCCGAAACCGTGGTCGAGGTGCAGTTCCACGTCACGCTGCTGGCGGTGCGCAGCGAGGGC
GCCGCGGGTCCGGCGATCGAATTCTGCTCACCGATCGGGCATCGCGGCGCCGGCGGGCGGGTGCTGGAATCCTGGCAGCC
GCAGAAGATGAGCCCGGCCGCGATGGACGCCGCGAAGTCGATCGCGGCGCGAATCGTCAAGGCGCTGGGCGGGCGTGGCG
TGTTCGGCGTCGAGTTGATGGTCAACGACGACGAGGTGTATTTCGCCGACGTCACCGCCCACCCGGGCGACAGCGCCTGG
GTGACGGTGCGCAGCCAGCGGCTCTCGGCGTTCGAACTGCAGGCCCGCACCATCCTGGGCCTGCCGGTGGACACCATGAT
GGTGTCGCCGGCCGCCGCCCGGGTGGTCGACTCCGCCGACCCCGGCGACCGAGCGGCCCTGTCCGGCGCGCTCGGCGTGC
CGGAGAGCGACCTGCGGGTGTGCGGGGGCGGTTTCCGGGCCCTGGCGACCGCGCCGGAGGTGGCCGCCGCCCGGGAGCGC
GCCGGCCAGGTCGCCGCCCGGCTGACCGCGGGCGCGGGCGGCGTGGTCGGCGCGGGCGGCTGA

Upstream 100 bases:

>100_bases
CCGAGGGCCACGGCCTGATGGTGCGGCTGCTGCCCGGCCAGCCCTGAGGCCGGCCCCGAGGCGGGACGGGCACTCAGCGG
ACCCACCTATCCTTGGACCC

Downstream 100 bases:

>100_bases
GCCGCGGCGGCGCCGGCGCGACGGCCTCGATACGATCGCAGGTCAGGGTGGCCGGCAATGCGTACCGAGACGAGGGGACA
CCAACATGAGCACCGAGCGC

Product: phosphoribosylglycinamide formyltransferase 2

Products: 5'-Phosphoribosyl-N-Formylglycinamide; Pyrophosphate; acetylphosphate; ADP [C]

Alternate protein names: GART 2; 5'-phosphoribosylglycinamide transformylase 2; Formate-dependent GAR transformylase; GAR transformylase 2 [H]

Number of amino acids: Translated: 420; Mature: 419

Protein sequence:

>420_residues
MTDGVTDGRDDETTALAVPRPAPADRGPAPADGSPAPADGRPAVMLLGSGDISRELAIALGRLGARVIAVDAHPQAPAHA
VADQALVLPSTDAGELSAAIHRLRPDVVVSTTDAVAVPALEALDGTGAEPVPSARAVRLAADREGLRRLAADELGLPTAP
FWFAGSLGELEAVGAHAGYPLLVKPVVGAVGPRQSVVAGREDIAAAWHRAVDGQPGARVLAETVVEVQFHVTLLAVRSEG
AAGPAIEFCSPIGHRGAGGRVLESWQPQKMSPAAMDAAKSIAARIVKALGGRGVFGVELMVNDDEVYFADVTAHPGDSAW
VTVRSQRLSAFELQARTILGLPVDTMMVSPAAARVVDSADPGDRAALSGALGVPESDLRVCGGGFRALATAPEVAAARER
AGQVAARLTAGAGGVVGAGG

Sequences:

>Translated_420_residues
MTDGVTDGRDDETTALAVPRPAPADRGPAPADGSPAPADGRPAVMLLGSGDISRELAIALGRLGARVIAVDAHPQAPAHA
VADQALVLPSTDAGELSAAIHRLRPDVVVSTTDAVAVPALEALDGTGAEPVPSARAVRLAADREGLRRLAADELGLPTAP
FWFAGSLGELEAVGAHAGYPLLVKPVVGAVGPRQSVVAGREDIAAAWHRAVDGQPGARVLAETVVEVQFHVTLLAVRSEG
AAGPAIEFCSPIGHRGAGGRVLESWQPQKMSPAAMDAAKSIAARIVKALGGRGVFGVELMVNDDEVYFADVTAHPGDSAW
VTVRSQRLSAFELQARTILGLPVDTMMVSPAAARVVDSADPGDRAALSGALGVPESDLRVCGGGFRALATAPEVAAARER
AGQVAARLTAGAGGVVGAGG
>Mature_419_residues
TDGVTDGRDDETTALAVPRPAPADRGPAPADGSPAPADGRPAVMLLGSGDISRELAIALGRLGARVIAVDAHPQAPAHAV
ADQALVLPSTDAGELSAAIHRLRPDVVVSTTDAVAVPALEALDGTGAEPVPSARAVRLAADREGLRRLAADELGLPTAPF
WFAGSLGELEAVGAHAGYPLLVKPVVGAVGPRQSVVAGREDIAAAWHRAVDGQPGARVLAETVVEVQFHVTLLAVRSEGA
AGPAIEFCSPIGHRGAGGRVLESWQPQKMSPAAMDAAKSIAARIVKALGGRGVFGVELMVNDDEVYFADVTAHPGDSAWV
TVRSQRLSAFELQARTILGLPVDTMMVSPAAARVVDSADPGDRAALSGALGVPESDLRVCGGGFRALATAPEVAAARERA
GQVAARLTAGAGGVVGAGG

Specific function: Catalyzes two reactions:the first one is the production of beta-formyl glycinamide ribonucleotide (GAR) from formate, ATP and beta GAR; the second, a side reaction, is the production of acetyl phosphate and ADP from acetate and ATP [H]

COG id: COG0027

COG function: function code F; Formate-dependent phosphoribosylglycinamide formyltransferase (GAR transformylase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1788155, Length=384, Percent_Identity=41.40625, Blast_Score=247, Evalue=1e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR005862
- InterPro:   IPR011054 [H]

Pfam domain/function: PF02222 ATP-grasp [H]

EC number: 2.1.2.- [C]

Molecular weight: Translated: 42474; Mature: 42343

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDGVTDGRDDETTALAVPRPAPADRGPAPADGSPAPADGRPAVMLLGSGDISRELAIAL
CCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHH
GRLGARVIAVDAHPQAPAHAVADQALVLPSTDAGELSAAIHRLRPDVVVSTTDAVAVPAL
HHCCCEEEEEECCCCCCHHHHCCCEEEECCCCHHHHHHHHHHCCCCEEEECCCCHHHHHH
EALDGTGAEPVPSARAVRLAADREGLRRLAADELGLPTAPFWFAGSLGELEAVGAHAGYP
HHHCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCHHCCCCCCCCC
LLVKPVVGAVGPRQSVVAGREDIAAAWHRAVDGQPGARVLAETVVEVQFHVTLLAVRSEG
EEEHHHHCCCCCCHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHEEEEEEEEEECCC
AAGPAIEFCSPIGHRGAGGRVLESWQPQKMSPAAMDAAKSIAARIVKALGGRGVFGVELM
CCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEE
VNDDEVYFADVTAHPGDSAWVTVRSQRLSAFELQARTILGLPVDTMMVSPAAARVVDSAD
ECCCEEEEEEEECCCCCCEEEEEECCCCCEEEEEEEEEECCCHHHHHCCHHHHHHHCCCC
PGDRAALSGALGVPESDLRVCGGGFRALATAPEVAAARERAGQVAARLTAGAGGVVGAGG
CCCHHHHCCCCCCCHHHHHCCCCCHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
TDGVTDGRDDETTALAVPRPAPADRGPAPADGSPAPADGRPAVMLLGSGDISRELAIAL
CCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHH
GRLGARVIAVDAHPQAPAHAVADQALVLPSTDAGELSAAIHRLRPDVVVSTTDAVAVPAL
HHCCCEEEEEECCCCCCHHHHCCCEEEECCCCHHHHHHHHHHCCCCEEEECCCCHHHHHH
EALDGTGAEPVPSARAVRLAADREGLRRLAADELGLPTAPFWFAGSLGELEAVGAHAGYP
HHHCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCHHCCCCCCCCC
LLVKPVVGAVGPRQSVVAGREDIAAAWHRAVDGQPGARVLAETVVEVQFHVTLLAVRSEG
EEEHHHHCCCCCCHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHEEEEEEEEEECCC
AAGPAIEFCSPIGHRGAGGRVLESWQPQKMSPAAMDAAKSIAARIVKALGGRGVFGVELM
CCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEE
VNDDEVYFADVTAHPGDSAWVTVRSQRLSAFELQARTILGLPVDTMMVSPAAARVVDSAD
ECCCEEEEEEEECCCCCCEEEEEECCCCCEEEEEEEEEECCCHHHHHCCHHHHHHHCCCC
PGDRAALSGALGVPESDLRVCGGGFRALATAPEVAAARERAGQVAARLTAGAGGVVGAGG
CCCHHHHCCCCCCCHHHHHCCCCCHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Formate; 5'-Phospho-Ribosylglycinamide; acetate; ATP [C]

Specific reaction: Formate + ATP + 5'-Phospho-Ribosylglycinamide = 5'-Phosphoribosyl-N-Formylglycinamide + ADP + Pyrophosphate. acetate + ATP = acetylphosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA