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The map label for this gene is rmlA

Identifier: 41409926

GI number: 41409926

Start: 4278770

End: 4279645

Strand: Direct

Name: rmlA

Synonym: MAP3828

Alternate gene names: 41409926

Gene position: 4278770-4279645 (Clockwise)

Preceding gene: 41409925

Following gene: 41409932

Centisome position: 88.59

GC content: 64.38

Gene sequence:

>876_bases
ATGCGCGGAATCATCTTGGCCGGCGGGTCGGGCACCCGCCTGCATCCGATCACCACCGGCATCAGCAAGCAGTTGCTGCC
GGTCTACGACAAGCCGATGGTCTACTACCCGCTGTCCACCCTGATGATGGCCGGCATCCGCGACATCCTGGTGATCACCA
CCCCGCACGACGCGGCCGGATTCGTCCGGCTGCTGGGCGATGGCTCGCAATTCGGCATCAACATCACCTACGTGACGCAG
GAGCGGCCCGACGGCCTGGCCCAGGCGTTCGTCCTGGGCGCTGACCACATCGGCAACGACTCGGTGGCATTGGTGTTGGG
AGACAACATCTTCTACGGCCCCGGGCTGGGCACCAGCCTGAGGCGCTTCCAAACCATAACCGGCGGAGCGGTTTTCGCGT
ACTGGGTGGCCAACCCGTCGGCGTACGGGGTGGTGGAGTTCAGCGACGACGGCATGGCGCTGTCGCTGGAGGAGAAACCC
AAGACGCCGAAATCCAATTACGCGGTGCCGGGTTTGTATTTCTACGACAACGACGTGATCGAAATCGCCAAGGGCCTGAA
GAAATCCGAGCGCGGCGAGTACGAGATCACCGAGGTCAACCAGATCTATCTCAGCCGCGGCCGGCTCTCGGTCGAGGTGA
TGGCCCGCGGCACGGCCTGGCTGGACACCGGCACTTTCGATTCGCTGCTGGACGCCAGCGACTTCGTGCGCACGCTGGAG
CGTCGCCAGGGGCTCAAAGTCAGTGTGCCCGAGGAGGTGGCGTGGCAGCAGGGCTGGATCACCGACGAGCAGCTGTCCGA
GCGCGCCCAGAGCCTGCTCAAGTCGGGCTACGGCGGCTATCTGCTGGACCTGTTGGAGCGCAGCCGTTTTCACTAG

Upstream 100 bases:

>100_bases
GGTGTTCCGGCACGACAGCCTGGCCTCGGCGCTGGCGGCCACCGAACTGACCGAGGACGACGCCGTCGACTAGGGCCGAT
CAAGGCAGTAGGAGCCACCC

Downstream 100 bases:

>100_bases
GCTGATGGCGGCGGCCCGCCGCGCCCGGCTTCGCCGCGCTTGCGACCGCCGCTAGACCGATGGCGGCGGCCCGCCGCGCC
CGGCTTCGCCGCGCTTGCGA

Product: RmlA

Products: NA

Alternate protein names: dTDP-glucose pyrophosphorylase; dTDP-glucose synthase [H]

Number of amino acids: Translated: 291; Mature: 291

Protein sequence:

>291_residues
MRGIILAGGSGTRLHPITTGISKQLLPVYDKPMVYYPLSTLMMAGIRDILVITTPHDAAGFVRLLGDGSQFGINITYVTQ
ERPDGLAQAFVLGADHIGNDSVALVLGDNIFYGPGLGTSLRRFQTITGGAVFAYWVANPSAYGVVEFSDDGMALSLEEKP
KTPKSNYAVPGLYFYDNDVIEIAKGLKKSERGEYEITEVNQIYLSRGRLSVEVMARGTAWLDTGTFDSLLDASDFVRTLE
RRQGLKVSVPEEVAWQQGWITDEQLSERAQSLLKSGYGGYLLDLLERSRFH

Sequences:

>Translated_291_residues
MRGIILAGGSGTRLHPITTGISKQLLPVYDKPMVYYPLSTLMMAGIRDILVITTPHDAAGFVRLLGDGSQFGINITYVTQ
ERPDGLAQAFVLGADHIGNDSVALVLGDNIFYGPGLGTSLRRFQTITGGAVFAYWVANPSAYGVVEFSDDGMALSLEEKP
KTPKSNYAVPGLYFYDNDVIEIAKGLKKSERGEYEITEVNQIYLSRGRLSVEVMARGTAWLDTGTFDSLLDASDFVRTLE
RRQGLKVSVPEEVAWQQGWITDEQLSERAQSLLKSGYGGYLLDLLERSRFH
>Mature_291_residues
MRGIILAGGSGTRLHPITTGISKQLLPVYDKPMVYYPLSTLMMAGIRDILVITTPHDAAGFVRLLGDGSQFGINITYVTQ
ERPDGLAQAFVLGADHIGNDSVALVLGDNIFYGPGLGTSLRRFQTITGGAVFAYWVANPSAYGVVEFSDDGMALSLEEKP
KTPKSNYAVPGLYFYDNDVIEIAKGLKKSERGEYEITEVNQIYLSRGRLSVEVMARGTAWLDTGTFDSLLDASDFVRTLE
RRQGLKVSVPEEVAWQQGWITDEQLSERAQSLLKSGYGGYLLDLLERSRFH

Specific function: Catalyzes the formation of dTDP-glucose, from dTTP and glucose 1-phosphate, as well as its pyrophosphorolysis. Involved in the biosynthesis of the dTDP-L-rhamnose which is a component of the critical linker, D-N-acetylglucosamine-L-rhamnose disaccharide,

COG id: COG1209

COG function: function code M; dTDP-glucose pyrophosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glucose-1-phosphate thymidylyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1790224, Length=290, Percent_Identity=62.0689655172414, Blast_Score=369, Evalue=1e-103,
Organism=Escherichia coli, GI1788351, Length=289, Percent_Identity=61.2456747404844, Blast_Score=365, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI133931050, Length=185, Percent_Identity=27.5675675675676, Blast_Score=68, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6320148, Length=255, Percent_Identity=26.6666666666667, Blast_Score=77, Evalue=3e-15,
Organism=Drosophila melanogaster, GI21355443, Length=251, Percent_Identity=24.3027888446215, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24644084, Length=251, Percent_Identity=24.3027888446215, Blast_Score=72, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005907
- InterPro:   IPR005835 [H]

Pfam domain/function: PF00483 NTP_transferase [H]

EC number: =2.7.7.24 [H]

Molecular weight: Translated: 31999; Mature: 31999

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRGIILAGGSGTRLHPITTGISKQLLPVYDKPMVYYPLSTLMMAGIRDILVITTPHDAAG
CCEEEEECCCCCEEEEEECCCCCCCCCCCCCCEEEECHHHHHHHCCCEEEEEECCCCCCC
FVRLLGDGSQFGINITYVTQERPDGLAQAFVLGADHIGNDSVALVLGDNIFYGPGLGTSL
EEEEECCCCEEEEEEEEEECCCCCHHHHHEEECCCCCCCCCEEEEECCCEEECCCCCHHH
RRFQTITGGAVFAYWVANPSAYGVVEFSDDGMALSLEEKPKTPKSNYAVPGLYFYDNDVI
HHHHEECCCEEEEEEECCCCEEEEEEECCCCEEEEECCCCCCCCCCCCCCEEEEECCCHH
EIAKGLKKSERGEYEITEVNQIYLSRGRLSVEVMARGTAWLDTGTFDSLLDASDFVRTLE
HHHHHHHHCCCCCEEEEEHHEEEEECCCEEEEEEECCCEEEECCCHHHHHCHHHHHHHHH
RRQGLKVSVPEEVAWQQGWITDEQLSERAQSLLKSGYGGYLLDLLERSRFH
HHCCCEEECCHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MRGIILAGGSGTRLHPITTGISKQLLPVYDKPMVYYPLSTLMMAGIRDILVITTPHDAAG
CCEEEEECCCCCEEEEEECCCCCCCCCCCCCCEEEECHHHHHHHCCCEEEEEECCCCCCC
FVRLLGDGSQFGINITYVTQERPDGLAQAFVLGADHIGNDSVALVLGDNIFYGPGLGTSL
EEEEECCCCEEEEEEEEEECCCCCHHHHHEEECCCCCCCCCEEEEECCCEEECCCCCHHH
RRFQTITGGAVFAYWVANPSAYGVVEFSDDGMALSLEEKPKTPKSNYAVPGLYFYDNDVI
HHHHEECCCEEEEEEECCCCEEEEEEECCCCEEEEECCCCCCCCCCCCCCEEEEECCCHH
EIAKGLKKSERGEYEITEVNQIYLSRGRLSVEVMARGTAWLDTGTFDSLLDASDFVRTLE
HHHHHHHHCCCCCEEEEEHHEEEEECCCEEEEEEECCCEEEECCCHHHHHCHHHHHHHHH
RRQGLKVSVPEEVAWQQGWITDEQLSERAQSLLKSGYGGYLLDLLERSRFH
HHCCCEEECCHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9084178; 9634230; 12218036 [H]