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The map label for this gene is ilvB_2

Identifier: 41409773

GI number: 41409773

Start: 4082804

End: 4084324

Strand: Direct

Name: ilvB_2

Synonym: MAP3675

Alternate gene names: 41409773

Gene position: 4082804-4084324 (Clockwise)

Preceding gene: 41409772

Following gene: 41409774

Centisome position: 84.53

GC content: 67.78

Gene sequence:

>1521_bases
GTGCTGACCCGGCACGAGCAGGCCGCCTCCTTCATGGCCGAGATGTACGGACGGGTCACCGGCCGGGCGGCGGTGGTGTC
GGCCACGCTGGGGCCCGGCGCGATCAACATGCAACTCGGGGTCGCCGACGCCACCACCAACAGCACCCCGCTGGTCGCCA
TCTCCGCGCAGGTGGGTCACGACCGGGAGTTCAAGGAGTCGCATCAATACGTCGACCTGGTGTCGATGTTCGCCCCGATC
ACCCGGTGGGCGGCCGGCATCCCGACCGCGCGCGCCATCCCCGAGATGGTGCGCAAGGCCTTCAAACTCGCCGAATCCGA
GCGCCCGGCCGCGGTGTATCTGGCGGTGCCCGAACACATCGATGCCGACGAGACCGACTACGACCTGGGGCCGTTGCCGC
GCAACGTGGTCCGGCCCGACGCCCCGTCACCGCGCCAGGTGGAACGCGCAGCGCAGATCCTGCGCAACGCCAAGCGCCCG
GTGGTGCTGGCCGGGCACGGCGCGGCGCGCGCCGATGCCACCAAGGCGCTGGTGCGCTTCTCCGAGGAGTTCGGCATCCA
GGTGGCCAACACCTTCCACGGCAAGGGTGTCATGCCCGACGACCACCCCAACAGCATCGGCACGCTGGGCTTCATGCGGC
ACGACTACGTCAACTTCGGGTTCGACCACGCCGACGCCGTCATCGCCGTCGGCTACGAATTGCAGGAGTTCGACCCGGTC
CGGATCAACCCGCAGGCCGACAAGAAGATCATCCACATGCACCGGTTCCCGGCCGAGGTGGACGCGCACTACTCGGTCGA
CGTGGGCATCATCGGCGACATCAGCGATTCGCTGAACGCGCTGACCGACGCGCTGTCCGGTCACCGTTTCGAGCACGCCG
CCGACGTGCCCGGCTCCGGGCTGCTGACCGACGAATTCGCGCGCGGGCAACAGGATTCGCGTTATCCGCTGGCCCCGGCG
CGGGTGGTCGCCGACACCCGGGCGGCGCTGGGCCGCAGCGACGTGGTGCTGGTCGACACCGGCGCCACCAAAATGTGGAT
GGCCCGGCTGTACCCGACCTACGAGCGCAACACCTGCCTGATCTCAAATGGCTTGTCCACCATGAGCTTTGCCCTGCCCG
GGGCGCTCGGGGTGAAGCTTGCCCAGCCGGAGTCCAGGGTGCTGGCCGTGGTCGGCGACGGCGCATTCCTGATGAACTCC
CAGGAGATCGAGACCGCCGTGCGCGAACGCATCCCGCTGGTGGTGCTGATTTGGGAGGACGGCGGCTACGGCCTGATCGA
ATGGAAGATGGACCTCGAGCTCGGCGCACACTACTACGTCAAGTTCGCCAACCCCGACGTGGTCACCTACGCAGAAAGCT
TCGGCGCCAAGGGATATCGCATCTCTCATGCCGACGAACTGTTGCCGACGCTGCGGGCCGCGCTCGACGACGACGGCGTC
TCGCTGATCAGCTGCCCGGTCGACTACTCGGAGAACCTGCGGCTGACCAACCGCCTCGGTGAGCTCGACGAAACGCTGTG
A

Upstream 100 bases:

>100_bases
CAAGTGCCTGGAAAACGAGGGGGTTTCCGTCGTGTTCGGGCTGCCGGGCGAGGAGAACATCCGCTTCGTGTAGGCGCTGG
CCGCCTCACCGATCCGCTAC

Downstream 100 bases:

>100_bases
AAAGCCGCCGCGGCAGCGACGGTCATTGACAATGGGCTGATGCTGTTTCGTCAGCTGGAGTACTTCGTCGCGCTCGCCTC
CGAGCGTCACTTCGCGCGGG

Product: acetolactate synthase

Products: NA

Alternate protein names: ALS; Acetohydroxy-acid synthase [H]

Number of amino acids: Translated: 506; Mature: 506

Protein sequence:

>506_residues
MLTRHEQAASFMAEMYGRVTGRAAVVSATLGPGAINMQLGVADATTNSTPLVAISAQVGHDREFKESHQYVDLVSMFAPI
TRWAAGIPTARAIPEMVRKAFKLAESERPAAVYLAVPEHIDADETDYDLGPLPRNVVRPDAPSPRQVERAAQILRNAKRP
VVLAGHGAARADATKALVRFSEEFGIQVANTFHGKGVMPDDHPNSIGTLGFMRHDYVNFGFDHADAVIAVGYELQEFDPV
RINPQADKKIIHMHRFPAEVDAHYSVDVGIIGDISDSLNALTDALSGHRFEHAADVPGSGLLTDEFARGQQDSRYPLAPA
RVVADTRAALGRSDVVLVDTGATKMWMARLYPTYERNTCLISNGLSTMSFALPGALGVKLAQPESRVLAVVGDGAFLMNS
QEIETAVRERIPLVVLIWEDGGYGLIEWKMDLELGAHYYVKFANPDVVTYAESFGAKGYRISHADELLPTLRAALDDDGV
SLISCPVDYSENLRLTNRLGELDETL

Sequences:

>Translated_506_residues
MLTRHEQAASFMAEMYGRVTGRAAVVSATLGPGAINMQLGVADATTNSTPLVAISAQVGHDREFKESHQYVDLVSMFAPI
TRWAAGIPTARAIPEMVRKAFKLAESERPAAVYLAVPEHIDADETDYDLGPLPRNVVRPDAPSPRQVERAAQILRNAKRP
VVLAGHGAARADATKALVRFSEEFGIQVANTFHGKGVMPDDHPNSIGTLGFMRHDYVNFGFDHADAVIAVGYELQEFDPV
RINPQADKKIIHMHRFPAEVDAHYSVDVGIIGDISDSLNALTDALSGHRFEHAADVPGSGLLTDEFARGQQDSRYPLAPA
RVVADTRAALGRSDVVLVDTGATKMWMARLYPTYERNTCLISNGLSTMSFALPGALGVKLAQPESRVLAVVGDGAFLMNS
QEIETAVRERIPLVVLIWEDGGYGLIEWKMDLELGAHYYVKFANPDVVTYAESFGAKGYRISHADELLPTLRAALDDDGV
SLISCPVDYSENLRLTNRLGELDETL
>Mature_506_residues
MLTRHEQAASFMAEMYGRVTGRAAVVSATLGPGAINMQLGVADATTNSTPLVAISAQVGHDREFKESHQYVDLVSMFAPI
TRWAAGIPTARAIPEMVRKAFKLAESERPAAVYLAVPEHIDADETDYDLGPLPRNVVRPDAPSPRQVERAAQILRNAKRP
VVLAGHGAARADATKALVRFSEEFGIQVANTFHGKGVMPDDHPNSIGTLGFMRHDYVNFGFDHADAVIAVGYELQEFDPV
RINPQADKKIIHMHRFPAEVDAHYSVDVGIIGDISDSLNALTDALSGHRFEHAADVPGSGLLTDEFARGQQDSRYPLAPA
RVVADTRAALGRSDVVLVDTGATKMWMARLYPTYERNTCLISNGLSTMSFALPGALGVKLAQPESRVLAVVGDGAFLMNS
QEIETAVRERIPLVVLIWEDGGYGLIEWKMDLELGAHYYVKFANPDVVTYAESFGAKGYRISHADELLPTLRAALDDDGV
SLISCPVDYSENLRLTNRLGELDETL

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=499, Percent_Identity=25.8517034068136, Blast_Score=138, Evalue=1e-32,
Organism=Homo sapiens, GI21361361, Length=515, Percent_Identity=23.8834951456311, Blast_Score=115, Evalue=1e-25,
Organism=Escherichia coli, GI87081685, Length=526, Percent_Identity=31.9391634980989, Blast_Score=215, Evalue=6e-57,
Organism=Escherichia coli, GI1790104, Length=504, Percent_Identity=27.7777777777778, Blast_Score=187, Evalue=2e-48,
Organism=Escherichia coli, GI1786717, Length=501, Percent_Identity=27.1457085828343, Blast_Score=176, Evalue=2e-45,
Organism=Escherichia coli, GI1787096, Length=510, Percent_Identity=24.9019607843137, Blast_Score=135, Evalue=5e-33,
Organism=Escherichia coli, GI1788716, Length=450, Percent_Identity=26, Blast_Score=107, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17531301, Length=443, Percent_Identity=27.765237020316, Blast_Score=129, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI17531299, Length=443, Percent_Identity=27.765237020316, Blast_Score=129, Evalue=5e-30,
Organism=Saccharomyces cerevisiae, GI6323755, Length=521, Percent_Identity=26.4875239923225, Blast_Score=183, Evalue=5e-47,
Organism=Saccharomyces cerevisiae, GI6320816, Length=501, Percent_Identity=24.9500998003992, Blast_Score=106, Evalue=8e-24,
Organism=Saccharomyces cerevisiae, GI6323073, Length=491, Percent_Identity=23.6252545824847, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI19922626, Length=498, Percent_Identity=28.3132530120482, Blast_Score=140, Evalue=3e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012782
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 55118; Mature: 55118

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS00141 ASP_PROTEASE ; PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTRHEQAASFMAEMYGRVTGRAAVVSATLGPGAINMQLGVADATTNSTPLVAISAQVGH
CCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCEEEEEEEEEECCCCCCCEEEEEECCCC
DREFKESHQYVDLVSMFAPITRWAAGIPTARAIPEMVRKAFKLAESERPAAVYLAVPEHI
CCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCC
DADETDYDLGPLPRNVVRPDAPSPRQVERAAQILRNAKRPVVLAGHGAARADATKALVRF
CCCCCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHH
SEEFGIQVANTFHGKGVMPDDHPNSIGTLGFMRHDYVNFGFDHADAVIAVGYELQEFDPV
HHHHCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCE
RINPQADKKIIHMHRFPAEVDAHYSVDVGIIGDISDSLNALTDALSGHRFEHAADVPGSG
EECCCCCCEEEEEECCCCCCCCCEEEEEEEEECCHHHHHHHHHHHCCCCCCCCCCCCCCC
LLTDEFARGQQDSRYPLAPARVVADTRAALGRSDVVLVDTGATKMWMARLYPTYERNTCL
CCCHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHCCCCCCCEEE
ISNGLSTMSFALPGALGVKLAQPESRVLAVVGDGAFLMNSQEIETAVRERIPLVVLIWED
EECCCCHHHHHCCCHHCEEEECCCCEEEEEEECCEEEECCHHHHHHHHHCCCEEEEEEEC
GGYGLIEWKMDLELGAHYYVKFANPDVVTYAESFGAKGYRISHADELLPTLRAALDDDGV
CCEEEEEEEEEEEECCEEEEEECCCCEEEEHHHCCCCCEEECCHHHHHHHHHHHCCCCCC
SLISCPVDYSENLRLTNRLGELDETL
EEEECCCCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MLTRHEQAASFMAEMYGRVTGRAAVVSATLGPGAINMQLGVADATTNSTPLVAISAQVGH
CCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCEEEEEEEEEECCCCCCCEEEEEECCCC
DREFKESHQYVDLVSMFAPITRWAAGIPTARAIPEMVRKAFKLAESERPAAVYLAVPEHI
CCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCC
DADETDYDLGPLPRNVVRPDAPSPRQVERAAQILRNAKRPVVLAGHGAARADATKALVRF
CCCCCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHH
SEEFGIQVANTFHGKGVMPDDHPNSIGTLGFMRHDYVNFGFDHADAVIAVGYELQEFDPV
HHHHCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCE
RINPQADKKIIHMHRFPAEVDAHYSVDVGIIGDISDSLNALTDALSGHRFEHAADVPGSG
EECCCCCCEEEEEECCCCCCCCCEEEEEEEEECCHHHHHHHHHHHCCCCCCCCCCCCCCC
LLTDEFARGQQDSRYPLAPARVVADTRAALGRSDVVLVDTGATKMWMARLYPTYERNTCL
CCCHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHCCCCCCCEEE
ISNGLSTMSFALPGALGVKLAQPESRVLAVVGDGAFLMNSQEIETAVRERIPLVVLIWED
EECCCCHHHHHCCCHHCEEEECCCCEEEEEEECCEEEECCHHHHHHHHHCCCEEEEEEEC
GGYGLIEWKMDLELGAHYYVKFANPDVVTYAESFGAKGYRISHADELLPTLRAALDDDGV
CCEEEEEEEEEEEECCEEEEEECCCCEEEEHHHCCCCCEEECCHHHHHHHHHHHCCCCCC
SLISCPVDYSENLRLTNRLGELDETL
EEEECCCCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7685336; 9353933; 9384377; 10809684 [H]