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The map label for this gene is sigF_2

Identifier: 41409504

GI number: 41409504

Start: 3781604

End: 3782392

Strand: Reverse

Name: sigF_2

Synonym: MAP3406c

Alternate gene names: 41409504

Gene position: 3782392-3781604 (Counterclockwise)

Preceding gene: 41409505

Following gene: 41409496

Centisome position: 78.31

GC content: 68.69

Gene sequence:

>789_bases
GTGACAGCGCGAGCTGCCGGCGGTTCGGTTTCTCGCCCGAACGAATACGCCGATGTCCCGGACATGTTCCGTGAGTTGGC
CACTGCCGAGCCCGACTCGATGGAGTTTCAGCGTCAACGGGACAAGATCGTCGAGCGGTGCCTGCCGCTGGCCGACCACA
TTGCGCGCCGCTTCGAGGGCCGCGGCGAGCCCCGCGACGACCTGGTTCAGGTGGCCCGGGTCGGCCTGGTCAACGCGGTG
GTCCGGTTCGACGTCGACGCCGGCTCGGACTTCGTCTCGTTCGCGGTGCCCACCATCATGGGTGAGGTTCGCCGGCACTT
CCGGGACAACAGCTGGTCGGTGAAGGTGCCGCGGCGGCTCAAGGAGCTGCACCTGCGGCTGGGCACCGCGACCGCCGACC
TGTCGCAGCGGCTGGGCCGGGCGCCGACCGCGACCGAGCTGGCCGCGGAGCTGGAGATGGACCGCGAAGAGGTGGTCGAG
GGGCTGGTCGCCGGCAGCTCCTACAACACCTTGTCCATCGACAGCGGCGGCGGCAGCGAAGAGGAGGAAGTGCGCGCGAT
CGCCGACACGCTGGGCGACGTGGACACCGGGCTGGACCGGATCGAGGACCAGGAGTCGCTGCGGCCGTTGCTCGAGGCGC
TGCCCGAACGCGAACGCACCGTGCTGGTGTTGCGGTTCTTCGAGTCGATGACCCAAACCCAGATCGCCGAGCGGGTGGGC
ATCTCGCAGATGCACGTGTCGCGGCTGCTGGCCAAGTCGCTAACGCGGCTTCGTGACCAATTGCAGTAG

Upstream 100 bases:

>100_bases
TGGCCGACGACGTTCAGACTTTCCATGACGGTCGCGAACCCAACGAAGCCGGCAGTGTCTTCGGCATCACACTGACGGCG
CGACGGGCGGCCTCCAGCAG

Downstream 100 bases:

>100_bases
CGGCCCGGGCGATTCGTCGTCGTTGGCCCGCCGCGGCTCCAGCAGCGGCTCGGCCTTCGGGGTGGTCACCGGCAAGGTGC
CGTCCGGGTCGCACAGCCGC

Product: RNA polymerase sigma factor SigF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 262; Mature: 261

Protein sequence:

>262_residues
MTARAAGGSVSRPNEYADVPDMFRELATAEPDSMEFQRQRDKIVERCLPLADHIARRFEGRGEPRDDLVQVARVGLVNAV
VRFDVDAGSDFVSFAVPTIMGEVRRHFRDNSWSVKVPRRLKELHLRLGTATADLSQRLGRAPTATELAAELEMDREEVVE
GLVAGSSYNTLSIDSGGGSEEEEVRAIADTLGDVDTGLDRIEDQESLRPLLEALPERERTVLVLRFFESMTQTQIAERVG
ISQMHVSRLLAKSLTRLRDQLQ

Sequences:

>Translated_262_residues
MTARAAGGSVSRPNEYADVPDMFRELATAEPDSMEFQRQRDKIVERCLPLADHIARRFEGRGEPRDDLVQVARVGLVNAV
VRFDVDAGSDFVSFAVPTIMGEVRRHFRDNSWSVKVPRRLKELHLRLGTATADLSQRLGRAPTATELAAELEMDREEVVE
GLVAGSSYNTLSIDSGGGSEEEEVRAIADTLGDVDTGLDRIEDQESLRPLLEALPERERTVLVLRFFESMTQTQIAERVG
ISQMHVSRLLAKSLTRLRDQLQ
>Mature_261_residues
TARAAGGSVSRPNEYADVPDMFRELATAEPDSMEFQRQRDKIVERCLPLADHIARRFEGRGEPRDDLVQVARVGLVNAVV
RFDVDAGSDFVSFAVPTIMGEVRRHFRDNSWSVKVPRRLKELHLRLGTATADLSQRLGRAPTATELAAELEMDREEVVEG
LVAGSSYNTLSIDSGGGSEEEEVRAIADTLGDVDTGLDRIEDQESLRPLLEALPERERTVLVLRFFESMTQTQIAERVGI
SQMHVSRLLAKSLTRLRDQLQ

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is required for normal spore maturation [H]

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. SigB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788231, Length=228, Percent_Identity=28.9473684210526, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1789098, Length=230, Percent_Identity=30, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1789448, Length=253, Percent_Identity=28.0632411067194, Blast_Score=75, Evalue=6e-15,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014322
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 29259; Mature: 29128

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTARAAGGSVSRPNEYADVPDMFRELATAEPDSMEFQRQRDKIVERCLPLADHIARRFEG
CCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
RGEPRDDLVQVARVGLVNAVVRFDVDAGSDFVSFAVPTIMGEVRRHFRDNSWSVKVPRRL
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHH
KELHLRLGTATADLSQRLGRAPTATELAAELEMDREEVVEGLVAGSSYNTLSIDSGGGSE
HHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHCCCCCCEEEECCCCCCC
EEEVRAIADTLGDVDTGLDRIEDQESLRPLLEALPERERTVLVLRFFESMTQTQIAERVG
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHC
ISQMHVSRLLAKSLTRLRDQLQ
HHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TARAAGGSVSRPNEYADVPDMFRELATAEPDSMEFQRQRDKIVERCLPLADHIARRFEG
CCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
RGEPRDDLVQVARVGLVNAVVRFDVDAGSDFVSFAVPTIMGEVRRHFRDNSWSVKVPRRL
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHH
KELHLRLGTATADLSQRLGRAPTATELAAELEMDREEVVEGLVAGSSYNTLSIDSGGGSE
HHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHCCCCCCEEEECCCCCCC
EEEVRAIADTLGDVDTGLDRIEDQESLRPLLEALPERERTVLVLRFFESMTQTQIAERVG
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHC
ISQMHVSRLLAKSLTRLRDQLQ
HHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7476207; 12000953 [H]