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The map label for this gene is ydaP [H]

Identifier: 41409353

GI number: 41409353

Start: 3614189

End: 3615982

Strand: Direct

Name: ydaP [H]

Synonym: MAP3255

Alternate gene names: 41409353

Gene position: 3614189-3615982 (Clockwise)

Preceding gene: 41409352

Following gene: 41409356

Centisome position: 74.83

GC content: 67.11

Gene sequence:

>1794_bases
ATGTCCAAACAAGAGGTTTCCGACTATCTGCTCGAGCGTTTGCGGGCGTGGGGCGTCGAGCATGTATTCGGGTACCCCGG
CGACGGCATCAACGGGCTGCTCGCCGCGTGGGGCCGCGCCGACAACAAGCCGCAGTTCATCCAGTCGCGGCACGAGGAGA
TGAGCGCCTTTCAGGCGGTCGGCTACGCGAAGTTCACCGGCCGGGTGGGCGTGTGCGCGGCGACCTCCGGCCCCGGCGCC
ATCCATCTGCTCAACGGCCTCTACGACGCCAAACTCGACCACGTCCCGGTGGTGGCCATCGTCGGCCAGACCAACCGCAC
CGCGATGGGCGGCAACTACCAGCAAGAGGTCGACCTGCTCAGCCTTTTCAAGGACGTCGCCAGCGACTACGTCCAAATGG
TCACCGTGCCCGAGCAACTGCCCAACGTGCTGGACCGCGCGATCCGCATCGCGATGACCCAGCGGGCCCCGACGGCGCTG
ATCATCCCGGCCGACGTGCAGGAGCTGCCGTACTCGCCGCCCGAGCACGCGTTCAAGATGGTGCCCTCCAGCCTGGGCAT
CGAATACCCGGCCATCGCCCCGGAGGACGCCGCCATCGCCCGCGCGGCCGAGATCCTCAACGCGGGCAAGAAGGTGGCCA
TGCTGGTCGGCACCGGCGCCCGCGGGGCGCACCACGAGCTGGCCGAGGTCGCCGACCTGCTCGGCGCCGGCGCGGCCAAG
GCGCTGTTGGGCAAGGACGTGCTGTCCGACGAATTGCCTTGGGTCACCGGCTCTATCGGGCTTTTGGGCACGCGGCCCAG
CTATGAGCTGATGCGCGACTGCGACACGTTGCTCACGGTCGGATCCAGCTTCCCCTACACGCAGTTCCTGCCGGAGTTCG
GGCAGTGTCGGGCGGTGCAGATCGACATCGACGGCCGGCTCATCGGGATGTGCTACCCCTACGAGGTGAACCTGGTCGCC
GACGCGAAGGCCGCACTGCGCGCGCTGATTCCGCACCTGAAGCGCAAGGAGGACCGGTCCTGGCGGGAAGGCATCGAGAA
GAACGTGGCCCGCTGGTGGGAGACCATGGACAAGGAGGCGATGGTCAGTGCGGACCCGATCAACCCGCTGCGGCTGTTCT
CCGAGCTGTCCCCGCTGCTGCCCGACAACGCCATCGTGACAGCCGATTCCGGCTCGTCGGCCAACTGGTACGCCCGCAAT
CTGCGCTTCCGCGGCAACATGCGCGGCTCGCTGTCGGGCACGCTGGCGACGATGGGCCCCGGGGTCCCGTACGGCATCGG
CGCCAAGTTCGGCCAGCCGGACCGGCCCGTCATCGTGTTCAGCGGTGACGGCGCCATGCAGATGAACGGCATGGCCGAAC
TGATCACCGTCAAGCGGTACTGGAACGAATGGAAAGATCCGCGGCTGATCATCGCGGTGCTGCACAACAACGACCTCAAC
CAGGTCACCTGGGAGATGCGGGCGATGGCCGGAGCGCCGAAATTCGCTGAGTCGCAAAACCTTCCGGATGTCGACTTCGC
CGCGTTCGCGGCCGGGCTGGGGTTGAACGCGATGGCCATCAAGGATCCCGACGAGTTGTCCGACGCGTGGCGCAGCGCCC
TGTCGGCCGACCGTCCCACCGTGCTGGACGTCTACACCGATCCCGACATGCCGCCGATCCCGCCGCACGCCACCTGGGAG
CAGTTCAAGTCGGCGACCGCGGCGGTGCTCTCCGGCGACGAGGACCGCACCGGCTTCGTCAAGGTCGGCCTGAAAACCAA
GATGCAGGAGTTCTTGCCGCACAAGAAAAGCTGA

Upstream 100 bases:

>100_bases
ACGTGCCGAGGCCGCCGCCGCGGCGGCAGCCTGCCTGGTTCAACCACCAGCCACACACCCACCCGCCAGCACGGTCGGCC
GTTTGCCGAGGAGGGAAGAC

Downstream 100 bases:

>100_bases
CCGCGGCCGTTCAGCCCAGCGCGGAGCGGGCGCTGCGCAGGTCGTCGACGAACAACCGGTAGACCTCGTCGTGCCGTTCG
CTGCGGTCGCGCAGCACCGA

Product: thiamine pyrophosphate protein

Products: Acetate; CO2; Ubiquinol-8 [C]

Alternate protein names: NA

Number of amino acids: Translated: 597; Mature: 596

Protein sequence:

>597_residues
MSKQEVSDYLLERLRAWGVEHVFGYPGDGINGLLAAWGRADNKPQFIQSRHEEMSAFQAVGYAKFTGRVGVCAATSGPGA
IHLLNGLYDAKLDHVPVVAIVGQTNRTAMGGNYQQEVDLLSLFKDVASDYVQMVTVPEQLPNVLDRAIRIAMTQRAPTAL
IIPADVQELPYSPPEHAFKMVPSSLGIEYPAIAPEDAAIARAAEILNAGKKVAMLVGTGARGAHHELAEVADLLGAGAAK
ALLGKDVLSDELPWVTGSIGLLGTRPSYELMRDCDTLLTVGSSFPYTQFLPEFGQCRAVQIDIDGRLIGMCYPYEVNLVA
DAKAALRALIPHLKRKEDRSWREGIEKNVARWWETMDKEAMVSADPINPLRLFSELSPLLPDNAIVTADSGSSANWYARN
LRFRGNMRGSLSGTLATMGPGVPYGIGAKFGQPDRPVIVFSGDGAMQMNGMAELITVKRYWNEWKDPRLIIAVLHNNDLN
QVTWEMRAMAGAPKFAESQNLPDVDFAAFAAGLGLNAMAIKDPDELSDAWRSALSADRPTVLDVYTDPDMPPIPPHATWE
QFKSATAAVLSGDEDRTGFVKVGLKTKMQEFLPHKKS

Sequences:

>Translated_597_residues
MSKQEVSDYLLERLRAWGVEHVFGYPGDGINGLLAAWGRADNKPQFIQSRHEEMSAFQAVGYAKFTGRVGVCAATSGPGA
IHLLNGLYDAKLDHVPVVAIVGQTNRTAMGGNYQQEVDLLSLFKDVASDYVQMVTVPEQLPNVLDRAIRIAMTQRAPTAL
IIPADVQELPYSPPEHAFKMVPSSLGIEYPAIAPEDAAIARAAEILNAGKKVAMLVGTGARGAHHELAEVADLLGAGAAK
ALLGKDVLSDELPWVTGSIGLLGTRPSYELMRDCDTLLTVGSSFPYTQFLPEFGQCRAVQIDIDGRLIGMCYPYEVNLVA
DAKAALRALIPHLKRKEDRSWREGIEKNVARWWETMDKEAMVSADPINPLRLFSELSPLLPDNAIVTADSGSSANWYARN
LRFRGNMRGSLSGTLATMGPGVPYGIGAKFGQPDRPVIVFSGDGAMQMNGMAELITVKRYWNEWKDPRLIIAVLHNNDLN
QVTWEMRAMAGAPKFAESQNLPDVDFAAFAAGLGLNAMAIKDPDELSDAWRSALSADRPTVLDVYTDPDMPPIPPHATWE
QFKSATAAVLSGDEDRTGFVKVGLKTKMQEFLPHKKS
>Mature_596_residues
SKQEVSDYLLERLRAWGVEHVFGYPGDGINGLLAAWGRADNKPQFIQSRHEEMSAFQAVGYAKFTGRVGVCAATSGPGAI
HLLNGLYDAKLDHVPVVAIVGQTNRTAMGGNYQQEVDLLSLFKDVASDYVQMVTVPEQLPNVLDRAIRIAMTQRAPTALI
IPADVQELPYSPPEHAFKMVPSSLGIEYPAIAPEDAAIARAAEILNAGKKVAMLVGTGARGAHHELAEVADLLGAGAAKA
LLGKDVLSDELPWVTGSIGLLGTRPSYELMRDCDTLLTVGSSFPYTQFLPEFGQCRAVQIDIDGRLIGMCYPYEVNLVAD
AKAALRALIPHLKRKEDRSWREGIEKNVARWWETMDKEAMVSADPINPLRLFSELSPLLPDNAIVTADSGSSANWYARNL
RFRGNMRGSLSGTLATMGPGVPYGIGAKFGQPDRPVIVFSGDGAMQMNGMAELITVKRYWNEWKDPRLIIAVLHNNDLNQ
VTWEMRAMAGAPKFAESQNLPDVDFAAFAAGLGLNAMAIKDPDELSDAWRSALSADRPTVLDVYTDPDMPPIPPHATWEQ
FKSATAAVLSGDEDRTGFVKVGLKTKMQEFLPHKKS

Specific function: Unknown

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=584, Percent_Identity=23.458904109589, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI21361361, Length=562, Percent_Identity=22.9537366548043, Blast_Score=98, Evalue=2e-20,
Organism=Escherichia coli, GI1787096, Length=584, Percent_Identity=31.8493150684932, Blast_Score=283, Evalue=3e-77,
Organism=Escherichia coli, GI1790104, Length=561, Percent_Identity=27.0944741532977, Blast_Score=186, Evalue=4e-48,
Organism=Escherichia coli, GI87081685, Length=565, Percent_Identity=29.2035398230088, Blast_Score=182, Evalue=4e-47,
Organism=Escherichia coli, GI1786717, Length=561, Percent_Identity=25.1336898395722, Blast_Score=126, Evalue=4e-30,
Organism=Escherichia coli, GI1788716, Length=559, Percent_Identity=23.613595706619, Blast_Score=101, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17531299, Length=474, Percent_Identity=25.7383966244726, Blast_Score=101, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17531301, Length=474, Percent_Identity=25.7383966244726, Blast_Score=101, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6323755, Length=570, Percent_Identity=25.9649122807018, Blast_Score=157, Evalue=6e-39,
Organism=Saccharomyces cerevisiae, GI6320816, Length=574, Percent_Identity=23.1707317073171, Blast_Score=98, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6321524, Length=485, Percent_Identity=24.1237113402062, Blast_Score=73, Evalue=1e-13,
Organism=Drosophila melanogaster, GI19922626, Length=594, Percent_Identity=24.5791245791246, Blast_Score=124, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: 1.2.2.2 [C]

Molecular weight: Translated: 65043; Mature: 64912

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKQEVSDYLLERLRAWGVEHVFGYPGDGINGLLAAWGRADNKPQFIQSRHEEMSAFQAV
CCHHHHHHHHHHHHHHCCCHHCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
GYAKFTGRVGVCAATSGPGAIHLLNGLYDAKLDHVPVVAIVGQTNRTAMGGNYQQEVDLL
CHHEECCCEEEEEECCCCCHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCHHHHHHHH
SLFKDVASDYVQMVTVPEQLPNVLDRAIRIAMTQRAPTALIIPADVQELPYSPPEHAFKM
HHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHCCCCCHHHHHHH
VPSSLGIEYPAIAPEDAAIARAAEILNAGKKVAMLVGTGARGAHHELAEVADLLGAGAAK
CHHHHCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHCCCHHH
ALLGKDVLSDELPWVTGSIGLLGTRPSYELMRDCDTLLTVGSSFPYTQFLPEFGQCRAVQ
HHHHHHHHHCCCCEEECCCEEEECCCCHHHHHHHHHHHHCCCCCCHHHHHHHHCCCEEEE
IDIDGRLIGMCYPYEVNLVADAKAALRALIPHLKRKEDRSWREGIEKNVARWWETMDKEA
EEECCEEEEEECCEEEEEEECHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCHHH
MVSADPINPLRLFSELSPLLPDNAIVTADSGSSANWYARNLRFRGNMRGSLSGTLATMGP
CCCCCCCCHHHHHHHHHCCCCCCEEEEECCCCCCCEEEEEEEEECCCCCCCCCCHHHCCC
GVPYGIGAKFGQPDRPVIVFSGDGAMQMNGMAELITVKRYWNEWKDPRLIIAVLHNNDLN
CCCCCCCCCCCCCCCCEEEECCCCCEEECCHHHHHHHHHHHHCCCCCEEEEEEEECCCCH
QVTWEMRAMAGAPKFAESQNLPDVDFAAFAAGLGLNAMAIKDPDELSDAWRSALSADRPT
HHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCCEEEECCHHHHHHHHHHHHCCCCCE
VLDVYTDPDMPPIPPHATWEQFKSATAAVLSGDEDRTGFVKVGLKTKMQEFLPHKKS
EEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEECHHHHHHHHCCCCCC
>Mature Secondary Structure 
SKQEVSDYLLERLRAWGVEHVFGYPGDGINGLLAAWGRADNKPQFIQSRHEEMSAFQAV
CHHHHHHHHHHHHHHCCCHHCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
GYAKFTGRVGVCAATSGPGAIHLLNGLYDAKLDHVPVVAIVGQTNRTAMGGNYQQEVDLL
CHHEECCCEEEEEECCCCCHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCHHHHHHHH
SLFKDVASDYVQMVTVPEQLPNVLDRAIRIAMTQRAPTALIIPADVQELPYSPPEHAFKM
HHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHCCCCCHHHHHHH
VPSSLGIEYPAIAPEDAAIARAAEILNAGKKVAMLVGTGARGAHHELAEVADLLGAGAAK
CHHHHCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHCCCHHH
ALLGKDVLSDELPWVTGSIGLLGTRPSYELMRDCDTLLTVGSSFPYTQFLPEFGQCRAVQ
HHHHHHHHHCCCCEEECCCEEEECCCCHHHHHHHHHHHHCCCCCCHHHHHHHHCCCEEEE
IDIDGRLIGMCYPYEVNLVADAKAALRALIPHLKRKEDRSWREGIEKNVARWWETMDKEA
EEECCEEEEEECCEEEEEEECHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCHHH
MVSADPINPLRLFSELSPLLPDNAIVTADSGSSANWYARNLRFRGNMRGSLSGTLATMGP
CCCCCCCCHHHHHHHHHCCCCCCEEEEECCCCCCCEEEEEEEEECCCCCCCCCCHHHCCC
GVPYGIGAKFGQPDRPVIVFSGDGAMQMNGMAELITVKRYWNEWKDPRLIIAVLHNNDLN
CCCCCCCCCCCCCCCCEEEECCCCCEEECCHHHHHHHHHHHHCCCCCEEEEEEEECCCCH
QVTWEMRAMAGAPKFAESQNLPDVDFAAFAAGLGLNAMAIKDPDELSDAWRSALSADRPT
HHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCCEEEECCHHHHHHHHHHHHCCCCCE
VLDVYTDPDMPPIPPHATWEQFKSATAAVLSGDEDRTGFVKVGLKTKMQEFLPHKKS
EEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEECHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD oxidase,; Lipids site,; Phospholipids; Thiamine diphosphate [C]

Metal ions: Mg2+ [C]

Kcat value (1/min): 87000 [C]

Specific activity: 200

Km value (mM): 0.0017 {lecithin}} 0.03 {sodium} 11 {pyruvate}} 10 {pyruvate}} 0.0022 {lysolecithin}} 2 {ferricyanide}} 0.0012 {thiamine} [C]

Substrates: H2O; Pyruvate; Ubiquinone-8 [C]

Specific reaction: H2O + Pyruvate + Ubiquinone-8 --> Acetate + CO2 + Ubiquinol-8 [C]

General reaction: Oxidative decarboxylation; Redox reaction [C]

Inhibitor: 2, 3-Butanedione; Cu2+; Methyl methanethio sulfonate; N-Ethyl maleimide diphosphate; p-Chloromercuribenzoate; Phenyl glyoxal [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]