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The map label for this gene is eno [H]

Identifier: 41409231

GI number: 41409231

Start: 3486972

End: 3488255

Strand: Reverse

Name: eno [H]

Synonym: MAP3133c

Alternate gene names: 41409231

Gene position: 3488255-3486972 (Counterclockwise)

Preceding gene: 41409232

Following gene: 41409224

Centisome position: 72.22

GC content: 69.0

Gene sequence:

>1284_bases
ATGGTGCGGATCGCTTCCGTCGTCGCCCGCCAACTGCTGGACTGCAAGGCCCGGCCGCTGGTCGAGGTCGAAATCACCAC
CGACACCGGCCATGTCGGCCGCGGCGCGGCGCCGACCGGCACCTCCGTCGGGGCGCACGAGGCGTTCGTCCTGCGGGACG
GGGACCCCACCCGATACCGCGGCCGCAGCGTGCACCGCGCCGTCGCCGCGGTCAGAGACGAAATCGCCCCCGCGCTGACC
GGCGCCGAACTGGACGACCCGCGGTCGCTGGACCGGGTCATGATCGAACTCGACGACACGCCCGACAAACACCGGCTGGG
CGGCAACGCGATCTACTCCACGTCGATCGCGCTGCTGCGCGCGGCCGCCGCGGCAGCGGGCACACCCACCTACACCTACG
TCGGCGCCCTGCTCGGGCTGACACCACCGACCACCGTGCCGATGCCCAGCTTCAACATGATCAACGGCGGCCGCTACGGG
GACGTCGAGCAGAGTTTCAGCGAGTTCCTCGTCGTGCCGTATCGGGCGGAGTCGATCCAGGCGGCGGTCGAGAAGGGGGT
GAGCCTGTTCGAGGTGCTCGGCGAGGTGCTCGCCGAACATCTGGGCCGCACACCGCTGTTGGCGTCGTCCTACGGCTATA
TCGCGCCGTCCGGTGACCCGCACGCCGTCCTGGAACTGCTGGCCGAGGCGGTCGAGCGCGCCGGCTGCGCCGACGTCATG
GCGTTCGCACTCGACTGCGCATCCAGCGAGGTGTGCGACAACGGTTCCGCCACTTACGCTTTCAACGGCGGCCGGGTGAC
CGCGGAGGCGTTGATCGACTACGCTCGCGCCCTGTCGCAGGAGTTCCCGATGCTGTTCATCGAGGATCTGCTGGACGGCG
ACGACTGGGCCGGGTTCACCAAAGCGGTGCAGACGGTCAACCGTTCGATCATCGTCGGGGACGATTTGATCGTGACCAAC
CGCGACCGGTTGCGGCGCGCCGTCGAGACGTCGGCCGTCGACGGGTTCATCCTCATGCCCAACCAGGTCGGCACCATCGC
CGAGGCGCTGGACTGCTTCGAATATGCCTCGCAGAACGGCCTTCTGGCGATTCCGTCCGGCCGGTCGGGCGGCGTCATCG
ACGACGTGGTGATGGATCTGGCGGTGGGGCTGGGTGCGCCGTTGCAGAAGAACGGCGCACCGCGCTCGGGCGAACGCATC
GAAAAGCTCAACTTCCTGCTGCGCGCGGCCGAGGGCATCCCGGACTGCGCGCTGGCGGATGTGCCGGCGCTGGCCCGGTT
CTAA

Upstream 100 bases:

>100_bases
GGAGGAGGAGACCCCCTAGGCGGGGAGTTCTCAGCGAGCTCTCAGCGGATTTGTCTTAGGCTGGCGCTCTCGATAGGCCT
CGACAGGGTCCGGGAGGTCG

Downstream 100 bases:

>100_bases
AAGCGGCGCTCTAGTGGCCGGCCAGCAACGCCAGCTCGGCCGCCACCGCGGCCGCGCACACCGCCGACGCGATCGAAAGC
GCTATCCAGTCATTGCGTTT

Product: hypothetical protein

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase [H]

Number of amino acids: Translated: 427; Mature: 427

Protein sequence:

>427_residues
MVRIASVVARQLLDCKARPLVEVEITTDTGHVGRGAAPTGTSVGAHEAFVLRDGDPTRYRGRSVHRAVAAVRDEIAPALT
GAELDDPRSLDRVMIELDDTPDKHRLGGNAIYSTSIALLRAAAAAAGTPTYTYVGALLGLTPPTTVPMPSFNMINGGRYG
DVEQSFSEFLVVPYRAESIQAAVEKGVSLFEVLGEVLAEHLGRTPLLASSYGYIAPSGDPHAVLELLAEAVERAGCADVM
AFALDCASSEVCDNGSATYAFNGGRVTAEALIDYARALSQEFPMLFIEDLLDGDDWAGFTKAVQTVNRSIIVGDDLIVTN
RDRLRRAVETSAVDGFILMPNQVGTIAEALDCFEYASQNGLLAIPSGRSGGVIDDVVMDLAVGLGAPLQKNGAPRSGERI
EKLNFLLRAAEGIPDCALADVPALARF

Sequences:

>Translated_427_residues
MVRIASVVARQLLDCKARPLVEVEITTDTGHVGRGAAPTGTSVGAHEAFVLRDGDPTRYRGRSVHRAVAAVRDEIAPALT
GAELDDPRSLDRVMIELDDTPDKHRLGGNAIYSTSIALLRAAAAAAGTPTYTYVGALLGLTPPTTVPMPSFNMINGGRYG
DVEQSFSEFLVVPYRAESIQAAVEKGVSLFEVLGEVLAEHLGRTPLLASSYGYIAPSGDPHAVLELLAEAVERAGCADVM
AFALDCASSEVCDNGSATYAFNGGRVTAEALIDYARALSQEFPMLFIEDLLDGDDWAGFTKAVQTVNRSIIVGDDLIVTN
RDRLRRAVETSAVDGFILMPNQVGTIAEALDCFEYASQNGLLAIPSGRSGGVIDDVVMDLAVGLGAPLQKNGAPRSGERI
EKLNFLLRAAEGIPDCALADVPALARF
>Mature_427_residues
MVRIASVVARQLLDCKARPLVEVEITTDTGHVGRGAAPTGTSVGAHEAFVLRDGDPTRYRGRSVHRAVAAVRDEIAPALT
GAELDDPRSLDRVMIELDDTPDKHRLGGNAIYSTSIALLRAAAAAAGTPTYTYVGALLGLTPPTTVPMPSFNMINGGRYG
DVEQSFSEFLVVPYRAESIQAAVEKGVSLFEVLGEVLAEHLGRTPLLASSYGYIAPSGDPHAVLELLAEAVERAGCADVM
AFALDCASSEVCDNGSATYAFNGGRVTAEALIDYARALSQEFPMLFIEDLLDGDDWAGFTKAVQTVNRSIIVGDDLIVTN
RDRLRRAVETSAVDGFILMPNQVGTIAEALDCFEYASQNGLLAIPSGRSGGVIDDVVMDLAVGLGAPLQKNGAPRSGERI
EKLNFLLRAAEGIPDCALADVPALARF

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis [H]

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the archaeal cel

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family [H]

Homologues:

Organism=Homo sapiens, GI301897477, Length=423, Percent_Identity=36.1702127659575, Blast_Score=259, Evalue=5e-69,
Organism=Homo sapiens, GI301897469, Length=423, Percent_Identity=36.1702127659575, Blast_Score=259, Evalue=5e-69,
Organism=Homo sapiens, GI5803011, Length=424, Percent_Identity=35.8490566037736, Blast_Score=249, Evalue=2e-66,
Organism=Homo sapiens, GI4503571, Length=418, Percent_Identity=34.2105263157895, Blast_Score=243, Evalue=2e-64,
Organism=Homo sapiens, GI301897479, Length=421, Percent_Identity=32.541567695962, Blast_Score=212, Evalue=4e-55,
Organism=Escherichia coli, GI1789141, Length=421, Percent_Identity=35.8669833729216, Blast_Score=258, Evalue=4e-70,
Organism=Caenorhabditis elegans, GI71995829, Length=417, Percent_Identity=33.8129496402878, Blast_Score=235, Evalue=3e-62,
Organism=Caenorhabditis elegans, GI17536383, Length=417, Percent_Identity=33.8129496402878, Blast_Score=235, Evalue=4e-62,
Organism=Caenorhabditis elegans, GI32563855, Length=218, Percent_Identity=31.651376146789, Blast_Score=104, Evalue=8e-23,
Organism=Saccharomyces cerevisiae, GI6321693, Length=425, Percent_Identity=35.0588235294118, Blast_Score=229, Evalue=9e-61,
Organism=Saccharomyces cerevisiae, GI6324974, Length=429, Percent_Identity=34.032634032634, Blast_Score=224, Evalue=2e-59,
Organism=Saccharomyces cerevisiae, GI6324969, Length=429, Percent_Identity=34.032634032634, Blast_Score=224, Evalue=2e-59,
Organism=Saccharomyces cerevisiae, GI6323985, Length=429, Percent_Identity=33.5664335664336, Blast_Score=221, Evalue=2e-58,
Organism=Saccharomyces cerevisiae, GI6321968, Length=427, Percent_Identity=34.4262295081967, Blast_Score=204, Evalue=3e-53,
Organism=Drosophila melanogaster, GI281360527, Length=419, Percent_Identity=35.3221957040573, Blast_Score=235, Evalue=4e-62,
Organism=Drosophila melanogaster, GI17137654, Length=419, Percent_Identity=35.3221957040573, Blast_Score=235, Evalue=4e-62,
Organism=Drosophila melanogaster, GI24580918, Length=419, Percent_Identity=35.3221957040573, Blast_Score=235, Evalue=5e-62,
Organism=Drosophila melanogaster, GI24580916, Length=419, Percent_Identity=35.3221957040573, Blast_Score=235, Evalue=5e-62,
Organism=Drosophila melanogaster, GI24580920, Length=419, Percent_Identity=35.3221957040573, Blast_Score=235, Evalue=5e-62,
Organism=Drosophila melanogaster, GI24580914, Length=419, Percent_Identity=35.3221957040573, Blast_Score=235, Evalue=5e-62,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811 [H]

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N [H]

EC number: =4.2.1.11 [H]

Molecular weight: Translated: 45191; Mature: 45191

Theoretical pI: Translated: 4.45; Mature: 4.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVRIASVVARQLLDCKARPLVEVEITTDTGHVGRGAAPTGTSVGAHEAFVLRDGDPTRYR
CCHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCC
GRSVHRAVAAVRDEIAPALTGAELDDPRSLDRVMIELDDTPDKHRLGGNAIYSTSIALLR
CHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCHHHCCCCHHHHHHHHHHH
AAAAAAGTPTYTYVGALLGLTPPTTVPMPSFNMINGGRYGDVEQSFSEFLVVPYRAESIQ
HHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHEEECCCHHHHH
AAVEKGVSLFEVLGEVLAEHLGRTPLLASSYGYIAPSGDPHAVLELLAEAVERAGCADVM
HHHHHCHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHH
AFALDCASSEVCDNGSATYAFNGGRVTAEALIDYARALSQEFPMLFIEDLLDGDDWAGFT
HHHHHHCCHHHCCCCCEEEEECCCEEEHHHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHH
KAVQTVNRSIIVGDDLIVTNRDRLRRAVETSAVDGFILMPNQVGTIAEALDCFEYASQNG
HHHHHHCCEEEECCCEEEECHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHCCCC
LLAIPSGRSGGVIDDVVMDLAVGLGAPLQKNGAPRSGERIEKLNFLLRAAEGIPDCALAD
EEEECCCCCCCHHHHHHHHHHHHCCCCHHCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHC
VPALARF
CCHHHCC
>Mature Secondary Structure
MVRIASVVARQLLDCKARPLVEVEITTDTGHVGRGAAPTGTSVGAHEAFVLRDGDPTRYR
CCHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCC
GRSVHRAVAAVRDEIAPALTGAELDDPRSLDRVMIELDDTPDKHRLGGNAIYSTSIALLR
CHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCHHHCCCCHHHHHHHHHHH
AAAAAAGTPTYTYVGALLGLTPPTTVPMPSFNMINGGRYGDVEQSFSEFLVVPYRAESIQ
HHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHEEECCCHHHHH
AAVEKGVSLFEVLGEVLAEHLGRTPLLASSYGYIAPSGDPHAVLELLAEAVERAGCADVM
HHHHHCHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHH
AFALDCASSEVCDNGSATYAFNGGRVTAEALIDYARALSQEFPMLFIEDLLDGDDWAGFT
HHHHHHCCHHHCCCCCEEEEECCCEEEHHHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHH
KAVQTVNRSIIVGDDLIVTNRDRLRRAVETSAVDGFILMPNQVGTIAEALDCFEYASQNG
HHHHHHCCEEEECCCEEEECHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHCCCC
LLAIPSGRSGGVIDDVVMDLAVGLGAPLQKNGAPRSGERIEKLNFLLRAAEGIPDCALAD
EEEECCCCCCCHHHHHHHHHHHHCCCCHHCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHC
VPALARF
CCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11930014 [H]