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The map label for this gene is gatB

Identifier: 41409140

GI number: 41409140

Start: 3383906

End: 3385417

Strand: Reverse

Name: gatB

Synonym: MAP3042c

Alternate gene names: 41409140

Gene position: 3385417-3383906 (Counterclockwise)

Preceding gene: 41409141

Following gene: 41409138

Centisome position: 70.09

GC content: 67.06

Gene sequence:

>1512_bases
ATGAGTGTTGCCGCCAACGCCGAGCTTATGGACTACGACGATGTCATCGCGCGTTTCGATCCGGTGCTCGGCCTCGAGGT
GCACGTCGAGCTGTCCACGGTGACCAAGATGTTCTGCGGCTGCACCACCGCGTTCGGCGCCGAACCCAACACCCAGGTGT
GCCCCGTGTGCCTGGGGCTGCCCGGCTCGCTGCCGGTGCTCAACCAGGCCGCGGTCGAGTCGGCCATCCGGATCGGGCTG
GCACTCAATTGCGAGATCGTGCCCTGGTGCCGGTTCGCCCGGAAGAACTACTTCTACCCGGACATGCCGAAGAACTACCA
GATTTCGCAGTACGACGAGCCCATCGCCATCAACGGCTACCTGGAGGCGCCGCTGGACGACGGCACCACCTGGCGGGTGG
ACATCGAACGCGCGCACATGGAGGAGGACACCGGCAAGCTCACCCACATCGGCAGCGAGACGGGCCGCATTCACGGCGCG
ACGACGTCGCTGATCGACTACAACCGTGCCGGTGTGCCGTTGATCGAGATCGTCACCAAACCCATCGTCGGCGCGGGAGA
GCGCGCACCGCAGATTGCCCGGGCGTATGTGACGGCTTTACGAGACCTGTTGCGCGCCTTGGGTGTATCCGACGTCCGGA
TGGATCAGGGCTCCATGCGCTGCGACGCCAACGTGTCGCTGAAACCGATCGGCACCGCCGAGTTCGGAACCCGCACCGAA
ACCAAGAACGTCAACTCGCTCAAGAGCGTCGAGGTGGCGGTGCGTTACGAAATGCAGCGCCAGGCCGCGGTTCTGGCCTC
GGGCGGCCGGATCACCCAGGAAACCCGCCACTTTCACGAAGCGGGCTACACCAGCCCCGGGCGCGTCAAGGAGACCGCCG
AGGACTACCGCTACTTCCCCGAGCCCGACCTGGAGCCCGTTGCGCCCAGCCGCGAACTGGTGGAGCGGCTGCGGCTGACC
ATCCCCGAGCTACCGTGGTTGAGCCGCAAGCGGATTCAGCAGGAGTGGGGCGTTTCCGACGAGGTGATGCGCGATCTGGT
CAACGCCGGCGCGGTGGACCTGGTCATCGAGACCGTCAAGCACGGCGCACCCAGTGAGCAGGCCCGCGCCTGGTGGGGAA
ACTTCTTGGTGCAAAAGGCCAATGAGGCCAACATCGGGCTGGACGAACTGAACATCAGCCCCGCCCAGGTGGCCGCGGTG
ATCGCCCTGGTCGACGAGGGCAAACTCTCGAACAAGCTGGCCCGCCAGGTCGTGGAAGGCGTGCTGGCCGGGGAGGGCGA
ACCCGACCAGGTGATGAACGCCCGCGGCCTCGAGCTGGTGCGCGACGACTCGGTCACCCAGGCCGCGGTCGACGAGGCGT
TGGCCGCCAATCCCGATGTGGCGGAGAAGATCCGCGGCGGCAAGATCGCGGCGGCCGGCGCGATCGTCGGGGCGGTGATG
AAGGCCACCCGCGGGCAGGCCGACGCCGCGCGGGTACGCGAACTGGTGCTGGCCGCCTGCGGCCAAGGCTAG

Upstream 100 bases:

>100_bases
GAAAGCCGGCGCAGCTACCTCGAGATGGCGGCCGCACTGGCCGATATGTTCCCGGGCAGGGGAGAGCAGCGCGGTCGCGG
TCCCCACTAGGATCGACGCC

Downstream 100 bases:

>100_bases
GCTGAGCACCCGCAGCTCGCCGAGCGTGTGGGAAAAGACGCACTAATCAACGAATTTCGTACCTAGGGCCCACGCTCGAC
GCGTCGACAGTTAGGTCTTG

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B [H]

Number of amino acids: Translated: 503; Mature: 502

Protein sequence:

>503_residues
MSVAANAELMDYDDVIARFDPVLGLEVHVELSTVTKMFCGCTTAFGAEPNTQVCPVCLGLPGSLPVLNQAAVESAIRIGL
ALNCEIVPWCRFARKNYFYPDMPKNYQISQYDEPIAINGYLEAPLDDGTTWRVDIERAHMEEDTGKLTHIGSETGRIHGA
TTSLIDYNRAGVPLIEIVTKPIVGAGERAPQIARAYVTALRDLLRALGVSDVRMDQGSMRCDANVSLKPIGTAEFGTRTE
TKNVNSLKSVEVAVRYEMQRQAAVLASGGRITQETRHFHEAGYTSPGRVKETAEDYRYFPEPDLEPVAPSRELVERLRLT
IPELPWLSRKRIQQEWGVSDEVMRDLVNAGAVDLVIETVKHGAPSEQARAWWGNFLVQKANEANIGLDELNISPAQVAAV
IALVDEGKLSNKLARQVVEGVLAGEGEPDQVMNARGLELVRDDSVTQAAVDEALAANPDVAEKIRGGKIAAAGAIVGAVM
KATRGQADAARVRELVLAACGQG

Sequences:

>Translated_503_residues
MSVAANAELMDYDDVIARFDPVLGLEVHVELSTVTKMFCGCTTAFGAEPNTQVCPVCLGLPGSLPVLNQAAVESAIRIGL
ALNCEIVPWCRFARKNYFYPDMPKNYQISQYDEPIAINGYLEAPLDDGTTWRVDIERAHMEEDTGKLTHIGSETGRIHGA
TTSLIDYNRAGVPLIEIVTKPIVGAGERAPQIARAYVTALRDLLRALGVSDVRMDQGSMRCDANVSLKPIGTAEFGTRTE
TKNVNSLKSVEVAVRYEMQRQAAVLASGGRITQETRHFHEAGYTSPGRVKETAEDYRYFPEPDLEPVAPSRELVERLRLT
IPELPWLSRKRIQQEWGVSDEVMRDLVNAGAVDLVIETVKHGAPSEQARAWWGNFLVQKANEANIGLDELNISPAQVAAV
IALVDEGKLSNKLARQVVEGVLAGEGEPDQVMNARGLELVRDDSVTQAAVDEALAANPDVAEKIRGGKIAAAGAIVGAVM
KATRGQADAARVRELVLAACGQG
>Mature_502_residues
SVAANAELMDYDDVIARFDPVLGLEVHVELSTVTKMFCGCTTAFGAEPNTQVCPVCLGLPGSLPVLNQAAVESAIRIGLA
LNCEIVPWCRFARKNYFYPDMPKNYQISQYDEPIAINGYLEAPLDDGTTWRVDIERAHMEEDTGKLTHIGSETGRIHGAT
TSLIDYNRAGVPLIEIVTKPIVGAGERAPQIARAYVTALRDLLRALGVSDVRMDQGSMRCDANVSLKPIGTAEFGTRTET
KNVNSLKSVEVAVRYEMQRQAAVLASGGRITQETRHFHEAGYTSPGRVKETAEDYRYFPEPDLEPVAPSRELVERLRLTI
PELPWLSRKRIQQEWGVSDEVMRDLVNAGAVDLVIETVKHGAPSEQARAWWGNFLVQKANEANIGLDELNISPAQVAAVI
ALVDEGKLSNKLARQVVEGVLAGEGEPDQVMNARGLELVRDDSVTQAAVDEALAANPDVAEKIRGGKIAAAGAIVGAVMK
ATRGQADAARVRELVLAACGQG

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758894, Length=501, Percent_Identity=34.9301397205589, Blast_Score=275, Evalue=9e-74,
Organism=Caenorhabditis elegans, GI115534094, Length=293, Percent_Identity=41.6382252559727, Blast_Score=210, Evalue=1e-54,
Organism=Saccharomyces cerevisiae, GI6319391, Length=312, Percent_Identity=33.6538461538462, Blast_Score=166, Evalue=6e-42,
Organism=Drosophila melanogaster, GI45550808, Length=494, Percent_Identity=30.1619433198381, Blast_Score=218, Evalue=7e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958 [H]

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey [H]

EC number: 6.3.5.-

Molecular weight: Translated: 54538; Mature: 54406

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVAANAELMDYDDVIARFDPVLGLEVHVELSTVTKMFCGCTTAFGAEPNTQVCPVCLGL
CCCCCCCCCCCHHHHHHHCCCCCCEEEEEEHHHHHHHHHCCHHHCCCCCCCCCCCEEECC
PGSLPVLNQAAVESAIRIGLALNCEIVPWCRFARKNYFYPDMPKNYQISQYDEPIAINGY
CCCCCHHHHHHHHHHHHHCEEECCEEEHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEECE
LEAPLDDGTTWRVDIERAHMEEDTGKLTHIGSETGRIHGATTSLIDYNRAGVPLIEIVTK
EECCCCCCCEEEEEEHHHHCCCCCCCEEEECCCCCEEECCCHHHEECCCCCCHHHHHHHH
PIVGAGERAPQIARAYVTALRDLLRALGVSDVRMDQGSMRCDANVSLKPIGTAEFGTRTE
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEECCCCCEECCCCEEEECCCCCCCCCCC
TKNVNSLKSVEVAVRYEMQRQAAVLASGGRITQETRHFHEAGYTSPGRVKETAEDYRYFP
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCHHHHHHHCCCCC
EPDLEPVAPSRELVERLRLTIPELPWLSRKRIQQEWGVSDEVMRDLVNAGAVDLVIETVK
CCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHH
HGAPSEQARAWWGNFLVQKANEANIGLDELNISPAQVAAVIALVDEGKLSNKLARQVVEG
CCCCCHHHHHHHHHHHHHCCCCCCCCHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHH
VLAGEGEPDQVMNARGLELVRDDSVTQAAVDEALAANPDVAEKIRGGKIAAAGAIVGAVM
HHCCCCCHHHHHCCCCCEEECCCCHHHHHHHHHHCCCCCHHHHHCCCCEEHHHHHHHHHH
KATRGQADAARVRELVLAACGQG
HHHCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SVAANAELMDYDDVIARFDPVLGLEVHVELSTVTKMFCGCTTAFGAEPNTQVCPVCLGL
CCCCCCCCCCHHHHHHHCCCCCCEEEEEEHHHHHHHHHCCHHHCCCCCCCCCCCEEECC
PGSLPVLNQAAVESAIRIGLALNCEIVPWCRFARKNYFYPDMPKNYQISQYDEPIAINGY
CCCCCHHHHHHHHHHHHHCEEECCEEEHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEECE
LEAPLDDGTTWRVDIERAHMEEDTGKLTHIGSETGRIHGATTSLIDYNRAGVPLIEIVTK
EECCCCCCCEEEEEEHHHHCCCCCCCEEEECCCCCEEECCCHHHEECCCCCCHHHHHHHH
PIVGAGERAPQIARAYVTALRDLLRALGVSDVRMDQGSMRCDANVSLKPIGTAEFGTRTE
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEECCCCCEECCCCEEEECCCCCCCCCCC
TKNVNSLKSVEVAVRYEMQRQAAVLASGGRITQETRHFHEAGYTSPGRVKETAEDYRYFP
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCHHHHHHHCCCCC
EPDLEPVAPSRELVERLRLTIPELPWLSRKRIQQEWGVSDEVMRDLVNAGAVDLVIETVK
CCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHH
HGAPSEQARAWWGNFLVQKANEANIGLDELNISPAQVAAVIALVDEGKLSNKLARQVVEG
CCCCCHHHHHHHHHHHHHCCCCCCCCHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHH
VLAGEGEPDQVMNARGLELVRDDSVTQAAVDEALAANPDVAEKIRGGKIAAAGAIVGAVM
HHCCCCCHHHHHCCCCCEEECCCCHHHHHHHHHHCCCCCHHHHHCCCCEEHHHHHHHHHH
KATRGQADAARVRELVLAACGQG
HHHCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]