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The map label for this gene is leuC

Identifier: 41409124

GI number: 41409124

Start: 3365846

End: 3367276

Strand: Reverse

Name: leuC

Synonym: MAP3026c

Alternate gene names: 41409124

Gene position: 3367276-3365846 (Counterclockwise)

Preceding gene: 41409127

Following gene: 41409123

Centisome position: 69.72

GC content: 68.62

Gene sequence:

>1431_bases
ATGGGAATCGACACGGGGACGACGGCCACGCCGCGCACGCTGGCCGAAAAAGTCTGGGACGACCACGTTGTGGTGTCCGG
CGGGGCCAACGAGCCGGACCTGATCTACATCGATCTGCACCTGGTGCACGAGGTCACCAGCCCGCAGGCGTTCGACGGCC
TGCGGCTGGCCGGCCGGCCGGTGCGGCGGCCCGATCTGACGCTGGCCACCGAGGACCACAACGTGCCCACCGTCGACATC
GACAAGCCGATCGCCGACCCGGTGTCGCGCACCCAGGTCGAGACATTGCGCCGAAATTGTGCCGAATTCGGCGTCCGGCT
GTACCCGATGGGCGACGCGGAGCAGGGCATCGTGCATGTCGTCGGGCCGCAGCTGGGCCTGACCCAACCGGGAATGACGG
TGGTGTGTGGGGATAGTCACACCTCGACGCACGGCGCATTCGGCGCACTGGCCATGGGAATTGGCACCTCGGAGGTCGAG
CACGTGCTGGCCACCCAGACGTTGCCGCTGCGGCCGTTCAAGACGATGGCGGTCAACGTCGACGGCGAGTTGCCGGCCGG
CGTGACGGCCAAGGACATCATCCTGGCGCTGATCGCCAAGATCGGCACCGGCGGCGGGCAGGGGCACGTCATCGAATACC
GCGGCAGCGCCATCGAATCGCTGTCGATGGAAGGCCGGATGACCGTGTGCAACATGAGCATCGAGGCCGGTGCCCGAGCG
GGGATGATCGCTCCCGACGACACCACCTACGAGTTCCTGCGGGACCGCCCGCACGCCCCCAAGGGCGCGCAATGGGATGC
GGCCATGCGCTATTGGCAGCAGCTGCGCACCGATCCCGGCGCGGTGTTCGACACCGAGGTCTATCTGGATGCCGCGTCGC
TGAGCCCGTTCGTGACGTGGGGCACAAACCCGGGTCAGGGCGTGCCGTTGGCTGCCGCGGTACCGGATCCCGAACTGATG
ACCGACGATGCCGAGCGTCAGGCCGCCGAGAAAGCGTTGGCATACATGGACCTTCGACCGGGCACGCCGATGCGCGACAT
CGCGGTCGACGCGGTGTTCGTCGGCTCTTGTACCAACGGTCGTATCGAGGATCTGCGGGTCGTCGCCGACGTGCTGCGCG
GCCGCAAGGTCGCGCCGGGGGTGCGGATGCTCGTGGTGCCCGGCTCGATGCGGGTGCGCGCGCAGGCCGAAGCCGAAGGG
CTGGGCGAGGTTTTCACCGCCGCGGGCGCCGAATGGCGGCAGGCGGGCTGCTCGATGTGTTTGGGAATGAATCCCGATCA
GCTGGCGCCGGGGGAGCGGTGCGCGGCGACCTCGAACCGCAACTTCGAAGGGCGCCAGGGCAAAGGCGGCCGCACGCATT
TGGTGTCTCCGGCCGTCGCCGCGGCCACGGCGGTGCGCGGCACATTGTCCGCTCCGGCCGATTTGGACTGA

Upstream 100 bases:

>100_bases
CTATGCTGTCTCACAACGAGATACTAGCGTCTCGCATTATGAGATCACCAGCCCGACGGATCGGCGAGCCGCGGACCAAG
CGAATTGAGGCGAGTTCGAG

Downstream 100 bases:

>100_bases
CGTATTCGACCGTGAAGGGATGAGCATGGAAGCATTTCACACCCACACCGGCATCGGTGTGCCGCTGCGGCGCTCCAATG
TCGACACCGACCAGATCATT

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 476; Mature: 475

Protein sequence:

>476_residues
MGIDTGTTATPRTLAEKVWDDHVVVSGGANEPDLIYIDLHLVHEVTSPQAFDGLRLAGRPVRRPDLTLATEDHNVPTVDI
DKPIADPVSRTQVETLRRNCAEFGVRLYPMGDAEQGIVHVVGPQLGLTQPGMTVVCGDSHTSTHGAFGALAMGIGTSEVE
HVLATQTLPLRPFKTMAVNVDGELPAGVTAKDIILALIAKIGTGGGQGHVIEYRGSAIESLSMEGRMTVCNMSIEAGARA
GMIAPDDTTYEFLRDRPHAPKGAQWDAAMRYWQQLRTDPGAVFDTEVYLDAASLSPFVTWGTNPGQGVPLAAAVPDPELM
TDDAERQAAEKALAYMDLRPGTPMRDIAVDAVFVGSCTNGRIEDLRVVADVLRGRKVAPGVRMLVVPGSMRVRAQAEAEG
LGEVFTAAGAEWRQAGCSMCLGMNPDQLAPGERCAATSNRNFEGRQGKGGRTHLVSPAVAAATAVRGTLSAPADLD

Sequences:

>Translated_476_residues
MGIDTGTTATPRTLAEKVWDDHVVVSGGANEPDLIYIDLHLVHEVTSPQAFDGLRLAGRPVRRPDLTLATEDHNVPTVDI
DKPIADPVSRTQVETLRRNCAEFGVRLYPMGDAEQGIVHVVGPQLGLTQPGMTVVCGDSHTSTHGAFGALAMGIGTSEVE
HVLATQTLPLRPFKTMAVNVDGELPAGVTAKDIILALIAKIGTGGGQGHVIEYRGSAIESLSMEGRMTVCNMSIEAGARA
GMIAPDDTTYEFLRDRPHAPKGAQWDAAMRYWQQLRTDPGAVFDTEVYLDAASLSPFVTWGTNPGQGVPLAAAVPDPELM
TDDAERQAAEKALAYMDLRPGTPMRDIAVDAVFVGSCTNGRIEDLRVVADVLRGRKVAPGVRMLVVPGSMRVRAQAEAEG
LGEVFTAAGAEWRQAGCSMCLGMNPDQLAPGERCAATSNRNFEGRQGKGGRTHLVSPAVAAATAVRGTLSAPADLD
>Mature_475_residues
GIDTGTTATPRTLAEKVWDDHVVVSGGANEPDLIYIDLHLVHEVTSPQAFDGLRLAGRPVRRPDLTLATEDHNVPTVDID
KPIADPVSRTQVETLRRNCAEFGVRLYPMGDAEQGIVHVVGPQLGLTQPGMTVVCGDSHTSTHGAFGALAMGIGTSEVEH
VLATQTLPLRPFKTMAVNVDGELPAGVTAKDIILALIAKIGTGGGQGHVIEYRGSAIESLSMEGRMTVCNMSIEAGARAG
MIAPDDTTYEFLRDRPHAPKGAQWDAAMRYWQQLRTDPGAVFDTEVYLDAASLSPFVTWGTNPGQGVPLAAAVPDPELMT
DDAERQAAEKALAYMDLRPGTPMRDIAVDAVFVGSCTNGRIEDLRVVADVLRGRKVAPGVRMLVVPGSMRVRAQAEAEGL
GEVFTAAGAEWRQAGCSMCLGMNPDQLAPGERCAATSNRNFEGRQGKGGRTHLVSPAVAAATAVRGTLSAPADLD

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=388, Percent_Identity=26.8041237113402, Blast_Score=115, Evalue=8e-26,
Organism=Homo sapiens, GI8659555, Length=465, Percent_Identity=24.9462365591398, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI41352693, Length=370, Percent_Identity=24.3243243243243, Blast_Score=84, Evalue=2e-16,
Organism=Escherichia coli, GI1786259, Length=459, Percent_Identity=62.5272331154684, Blast_Score=585, Evalue=1e-168,
Organism=Escherichia coli, GI1787531, Length=360, Percent_Identity=25.5555555555556, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI87081781, Length=363, Percent_Identity=25.3443526170799, Blast_Score=76, Evalue=5e-15,
Organism=Escherichia coli, GI2367097, Length=138, Percent_Identity=34.7826086956522, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI25149337, Length=378, Percent_Identity=27.5132275132275, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI32564738, Length=378, Percent_Identity=27.5132275132275, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI25149342, Length=317, Percent_Identity=27.4447949526814, Blast_Score=109, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17568399, Length=395, Percent_Identity=25.8227848101266, Blast_Score=94, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6321429, Length=454, Percent_Identity=59.4713656387665, Blast_Score=552, Evalue=1e-158,
Organism=Saccharomyces cerevisiae, GI6320440, Length=451, Percent_Identity=27.4944567627494, Blast_Score=149, Evalue=6e-37,
Organism=Saccharomyces cerevisiae, GI6322261, Length=402, Percent_Identity=27.1144278606965, Blast_Score=141, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6323335, Length=402, Percent_Identity=26.3681592039801, Blast_Score=127, Evalue=4e-30,
Organism=Drosophila melanogaster, GI161076999, Length=393, Percent_Identity=25.9541984732824, Blast_Score=122, Evalue=5e-28,
Organism=Drosophila melanogaster, GI281365315, Length=399, Percent_Identity=26.0651629072682, Blast_Score=122, Evalue=5e-28,
Organism=Drosophila melanogaster, GI17864292, Length=399, Percent_Identity=26.0651629072682, Blast_Score=122, Evalue=5e-28,
Organism=Drosophila melanogaster, GI28571643, Length=399, Percent_Identity=26.0651629072682, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI24645686, Length=370, Percent_Identity=24.5945945945946, Blast_Score=75, Evalue=7e-14,
Organism=Drosophila melanogaster, GI17137564, Length=370, Percent_Identity=24.5945945945946, Blast_Score=71, Evalue=1e-12,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 50415; Mature: 50284

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGIDTGTTATPRTLAEKVWDDHVVVSGGANEPDLIYIDLHLVHEVTSPQAFDGLRLAGRP
CCCCCCCCCCHHHHHHHHCCCCEEEECCCCCCCEEEEEEEEEECCCCCCHHCCHHHCCCC
VRRPDLTLATEDHNVPTVDIDKPIADPVSRTQVETLRRNCAEFGVRLYPMGDAEQGIVHV
CCCCCCEEEECCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHCCEEEEECCCCCCCEEEE
VGPQLGLTQPGMTVVCGDSHTSTHGAFGALAMGIGTSEVEHVLATQTLPLRPFKTMAVNV
ECCCCCCCCCCCEEEECCCCCCCCCCHHHHHHCCCHHHHHHHHHHCCCCCCCCEEEEEEE
DGELPAGVTAKDIILALIAKIGTGGGQGHVIEYRGSAIESLSMEGRMTVCNMSIEAGARA
CCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEECCCHHHHHCCCCCEEEEEEEHHCCCCC
GMIAPDDTTYEFLRDRPHAPKGAQWDAAMRYWQQLRTDPGAVFDTEVYLDAASLSPFVTW
CCCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCEEEE
GTNPGQGVPLAAAVPDPELMTDDAERQAAEKALAYMDLRPGTPMRDIAVDAVFVGSCTNG
CCCCCCCCEEEEECCCCHHCCCCHHHHHHHHHHHHEECCCCCCHHHHEEEEEEEECCCCC
RIEDLRVVADVLRGRKVAPGVRMLVVPGSMRVRAQAEAEGLGEVFTAAGAEWRQAGCSMC
CHHHHHHHHHHHCCCCCCCCCEEEEECCCCEEEECCCCCCHHHHHHHCCHHHHHHCCCEE
LGMNPDQLAPGERCAATSNRNFEGRQGKGGRTHLVSPAVAAATAVRGTLSAPADLD
ECCCCCCCCCCCHHCCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
GIDTGTTATPRTLAEKVWDDHVVVSGGANEPDLIYIDLHLVHEVTSPQAFDGLRLAGRP
CCCCCCCCCHHHHHHHHCCCCEEEECCCCCCCEEEEEEEEEECCCCCCHHCCHHHCCCC
VRRPDLTLATEDHNVPTVDIDKPIADPVSRTQVETLRRNCAEFGVRLYPMGDAEQGIVHV
CCCCCCEEEECCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHCCEEEEECCCCCCCEEEE
VGPQLGLTQPGMTVVCGDSHTSTHGAFGALAMGIGTSEVEHVLATQTLPLRPFKTMAVNV
ECCCCCCCCCCCEEEECCCCCCCCCCHHHHHHCCCHHHHHHHHHHCCCCCCCCEEEEEEE
DGELPAGVTAKDIILALIAKIGTGGGQGHVIEYRGSAIESLSMEGRMTVCNMSIEAGARA
CCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEECCCHHHHHCCCCCEEEEEEEHHCCCCC
GMIAPDDTTYEFLRDRPHAPKGAQWDAAMRYWQQLRTDPGAVFDTEVYLDAASLSPFVTW
CCCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCEEEE
GTNPGQGVPLAAAVPDPELMTDDAERQAAEKALAYMDLRPGTPMRDIAVDAVFVGSCTNG
CCCCCCCCEEEEECCCCHHCCCCHHHHHHHHHHHHEECCCCCCHHHHEEEEEEEECCCCC
RIEDLRVVADVLRGRKVAPGVRMLVVPGSMRVRAQAEAEGLGEVFTAAGAEWRQAGCSMC
CHHHHHHHHHHHCCCCCCCCCEEEEECCCCEEEECCCCCCHHHHHHHCCHHHHHHCCCEE
LGMNPDQLAPGERCAATSNRNFEGRQGKGGRTHLVSPAVAAATAVRGTLSAPADLD
ECCCCCCCCCCCHHCCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA