Click here to switch to the map view.

The map label for this gene is leuD

Identifier: 41409123

GI number: 41409123

Start: 3365224

End: 3365826

Strand: Reverse

Name: leuD

Synonym: MAP3025c

Alternate gene names: 41409123

Gene position: 3365826-3365224 (Counterclockwise)

Preceding gene: 41409124

Following gene: 41409122

Centisome position: 69.69

GC content: 60.86

Gene sequence:

>603_bases
ATGAGCATGGAAGCATTTCACACCCACACCGGCATCGGTGTGCCGCTGCGGCGCTCCAATGTCGACACCGACCAGATCAT
TCCGGCGGTGTATTTGAAGCGCGTAACCCGAACCGGTTTCGAGGACGGCTTGTTCGCCAGCTGGCGGTCGGATCCGTCAT
TCGTGCTCAACCTCAGCCCCTTTGACCGTGGCTCAGTCCTAGTCGCCGGACCGGATTTCGGGACGGGGTCGTCGCGCGAG
CACGCGGTGTGGGCACTGATGGACTACGGGTTCCGGGTGGTCATCTCGTCTCGATTCGGCGACATTTTCCGGGGCAACGC
CGGCAAGGCGGGTCTGCTGGCGGCCGAAGTTTCCCAGGACGGGGTGGAGCTGCTCTGGAAGCTCATCGAGCAGAGCCCCG
GCTTGGAAATCACTGCCAATCTTCAAGATCGAAATATCACCGCGGGAACGACGGTGCTGCCGTTCAAGATTGACGACCAC
ACCGCGTGGCGACTACTCGAAGGGCTCGACGATATAGCCCTTACGCTGCGGAAACTCGACAGAATCGAATCCTACGAAGC
GACGTATCCCGATTGGAAACCGCGCACTTCGCCCGTCGCGTGA

Upstream 100 bases:

>100_bases
CGCACGCATTTGGTGTCTCCGGCCGTCGCCGCGGCCACGGCGGTGCGCGGCACATTGTCCGCTCCGGCCGATTTGGACTG
ACGTATTCGACCGTGAAGGG

Downstream 100 bases:

>100_bases
ACCGCTGAGCCGGGCCGAATTTCTGCTCGATGGGCTAACTAAACCGGCCCGATTTGCGACGCCCTCCACCGGTCAAGGGC
GGCAACCGGATTGCGAAAAC

Product: isopropylmalate isomerase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 200; Mature: 199

Protein sequence:

>200_residues
MSMEAFHTHTGIGVPLRRSNVDTDQIIPAVYLKRVTRTGFEDGLFASWRSDPSFVLNLSPFDRGSVLVAGPDFGTGSSRE
HAVWALMDYGFRVVISSRFGDIFRGNAGKAGLLAAEVSQDGVELLWKLIEQSPGLEITANLQDRNITAGTTVLPFKIDDH
TAWRLLEGLDDIALTLRKLDRIESYEATYPDWKPRTSPVA

Sequences:

>Translated_200_residues
MSMEAFHTHTGIGVPLRRSNVDTDQIIPAVYLKRVTRTGFEDGLFASWRSDPSFVLNLSPFDRGSVLVAGPDFGTGSSRE
HAVWALMDYGFRVVISSRFGDIFRGNAGKAGLLAAEVSQDGVELLWKLIEQSPGLEITANLQDRNITAGTTVLPFKIDDH
TAWRLLEGLDDIALTLRKLDRIESYEATYPDWKPRTSPVA
>Mature_199_residues
SMEAFHTHTGIGVPLRRSNVDTDQIIPAVYLKRVTRTGFEDGLFASWRSDPSFVLNLSPFDRGSVLVAGPDFGTGSSREH
AVWALMDYGFRVVISSRFGDIFRGNAGKAGLLAAEVSQDGVELLWKLIEQSPGLEITANLQDRNITAGTTVLPFKIDDHT
AWRLLEGLDDIALTLRKLDRIESYEATYPDWKPRTSPVA

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786258, Length=195, Percent_Identity=44.6153846153846, Blast_Score=165, Evalue=2e-42,
Organism=Saccharomyces cerevisiae, GI6321429, Length=199, Percent_Identity=40.2010050251256, Blast_Score=132, Evalue=4e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004431
- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573 [H]

Pfam domain/function: PF00694 Aconitase_C [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 22152; Mature: 22021

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMEAFHTHTGIGVPLRRSNVDTDQIIPAVYLKRVTRTGFEDGLFASWRSDPSFVLNLSP
CCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCEEEECCC
FDRGSVLVAGPDFGTGSSREHAVWALMDYGFRVVISSRFGDIFRGNAGKAGLLAAEVSQD
CCCCCEEEECCCCCCCCCCCCEEEEEECCCEEEEEECCCCHHHCCCCCCCCEEEECCCCC
GVELLWKLIEQSPGLEITANLQDRNITAGTTVLPFKIDDHTAWRLLEGLDDIALTLRKLD
HHHHHHHHHHCCCCCEEEEECCCCCEECCCEEEEEEECCHHHHHHHHCHHHHHHHHHHHH
RIESYEATYPDWKPRTSPVA
HHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SMEAFHTHTGIGVPLRRSNVDTDQIIPAVYLKRVTRTGFEDGLFASWRSDPSFVLNLSP
CCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCEEEECCC
FDRGSVLVAGPDFGTGSSREHAVWALMDYGFRVVISSRFGDIFRGNAGKAGLLAAEVSQD
CCCCCEEEECCCCCCCCCCCCEEEEEECCCEEEEEECCCCHHHCCCCCCCCEEEECCCCC
GVELLWKLIEQSPGLEITANLQDRNITAGTTVLPFKIDDHTAWRLLEGLDDIALTLRKLD
HHHHHHHHHHCCCCCEEEEECCCCCEECCCEEEEEEECCHHHHHHHHCHHHHHHHHHHHH
RIESYEATYPDWKPRTSPVA
HHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA