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The map label for this gene is dacB

Identifier: 41409077

GI number: 41409077

Start: 3311102

End: 3311971

Strand: Direct

Name: dacB

Synonym: MAP2979

Alternate gene names: 41409077

Gene position: 3311102-3311971 (Clockwise)

Preceding gene: 41409071

Following gene: 41409082

Centisome position: 68.56

GC content: 72.3

Gene sequence:

>870_bases
ATGCGCAAGCTCTGGACCGCGGCCGCGGCGCTGCTGCTCGTCGGCGCCTGCGGTGCCCCCACCGCCGCGGCGGACAGCAC
CCAGCCGGCCGGCTCGATCCCGATTCCCGACGGCCCGGCGCAGACCTGGATCATCGCCGACCTGGACAGCGGTCAGGTGC
TGGCCGGCCGCGACCCCAACGTCGCGCATCCGCCGGCCAGCACCATCAAGACGCTGCTGGCCCAGGTGGTGCTGGACGAG
GTGAGCCTGGACGCCACCGTGGTCGCCGACGCCGCCGACACCCAGGTCGAATGCAACTGCGTCGGCATCAAGCCGGGCCG
CAGCTACACCGCGCGCCAGCTGCTCGACGCCCTGCTGCTGGTGTCCGGCAACGACGCCGCCAACACGCTGGCGCACATGC
TGGGCGGGCCGGAGGCGACGGTGGCCAAGATGAACGCCAAGGCGGCGGCGCTCGGCGCCACCAACACGCACGCGTCGACG
CCGTCCGGCCTGGACGGGCCCAACGGGTCGGGGTCGTCGACGGCGCACGACCTGGCCGTCATCTTCCGCGCGGCAATGGC
CAACCCGGTGTTCGCGCAGATCACCGCCGAGCCCTCGGCGATGTTCCCCGGCGACAACGGTCTGCAGCCGATCACCAACC
AGGACGAGCTGCTGCAGCGCTACCCCGGCGCGATCGGCGGCAAGACCGGGTTCACCAACGCGGCCCGCAAGACCTTCGTC
GGCGCCGCCGCGCGCGGGGGCCGGCGGCTGGTGATCGCCATGATGTACGGGCTGATCAAGGAGGGCGGGCCGTCGTACTG
GGATCAGGCGGCGACGTTGTTCGACTGGGGATTCGCCCAGAGCCCGCAGGCCAGCATCGGCTCGCTGTAA

Upstream 100 bases:

>100_bases
TGGATCTCCAGGCGGTCGGAGATCTCCTCCAGGCTCAACATGCATCGGACAGTAGACGAAAATCCCCTCCGAAATGGACC
GGAAGTTAGACTTTGCGGCC

Downstream 100 bases:

>100_bases
CACCGACGCTCGCCACCCCGCGCCACGCCGCCCCCGCCGCGAGCAGACACAAAAGCGCCCGATTCCCCGGCGTGTCGCGT
GCTTTTGCGTCTGCTCGCCA

Product: DacB

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5*; PBP-5* [H]

Number of amino acids: Translated: 289; Mature: 289

Protein sequence:

>289_residues
MRKLWTAAAALLLVGACGAPTAAADSTQPAGSIPIPDGPAQTWIIADLDSGQVLAGRDPNVAHPPASTIKTLLAQVVLDE
VSLDATVVADAADTQVECNCVGIKPGRSYTARQLLDALLLVSGNDAANTLAHMLGGPEATVAKMNAKAAALGATNTHAST
PSGLDGPNGSGSSTAHDLAVIFRAAMANPVFAQITAEPSAMFPGDNGLQPITNQDELLQRYPGAIGGKTGFTNAARKTFV
GAAARGGRRLVIAMMYGLIKEGGPSYWDQAATLFDWGFAQSPQASIGSL

Sequences:

>Translated_289_residues
MRKLWTAAAALLLVGACGAPTAAADSTQPAGSIPIPDGPAQTWIIADLDSGQVLAGRDPNVAHPPASTIKTLLAQVVLDE
VSLDATVVADAADTQVECNCVGIKPGRSYTARQLLDALLLVSGNDAANTLAHMLGGPEATVAKMNAKAAALGATNTHAST
PSGLDGPNGSGSSTAHDLAVIFRAAMANPVFAQITAEPSAMFPGDNGLQPITNQDELLQRYPGAIGGKTGFTNAARKTFV
GAAARGGRRLVIAMMYGLIKEGGPSYWDQAATLFDWGFAQSPQASIGSL
>Mature_289_residues
MRKLWTAAAALLLVGACGAPTAAADSTQPAGSIPIPDGPAQTWIIADLDSGQVLAGRDPNVAHPPASTIKTLLAQVVLDE
VSLDATVVADAADTQVECNCVGIKPGRSYTARQLLDALLLVSGNDAANTLAHMLGGPEATVAKMNAKAAALGATNTHAST
PSGLDGPNGSGSSTAHDLAVIFRAAMANPVFAQITAEPSAMFPGDNGLQPITNQDELLQRYPGAIGGKTGFTNAARKTFV
GAAARGGRRLVIAMMYGLIKEGGPSYWDQAATLFDWGFAQSPQASIGSL

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors. Required specifically for the synthesis of the spore form of peptidoglycan (cortex) [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Forespore outer membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1786851, Length=272, Percent_Identity=31.9852941176471, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI87082054, Length=239, Percent_Identity=30.9623430962343, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI87082022, Length=270, Percent_Identity=30, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1787062, Length=292, Percent_Identity=29.7945205479452, Blast_Score=78, Evalue=8e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 29431; Mature: 29431

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKLWTAAAALLLVGACGAPTAAADSTQPAGSIPIPDGPAQTWIIADLDSGQVLAGRDPN
CCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCEEECCCCC
VAHPPASTIKTLLAQVVLDEVSLDATVVADAADTQVECNCVGIKPGRSYTARQLLDALLL
CCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEEEEEEEECCCCCHHHHHHHHHHHH
VSGNDAANTLAHMLGGPEATVAKMNAKAAALGATNTHASTPSGLDGPNGSGSSTAHDLAV
CCCCCHHHHHHHHHCCCCHHHHHHCCCHHEECCCCCCCCCCCCCCCCCCCCCCHHHHHHH
IFRAAMANPVFAQITAEPSAMFPGDNGLQPITNQDELLQRYPGAIGGKTGFTNAARKTFV
HHHHHHCCCEEEEEECCCCEECCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCHHHHHHHH
GAAARGGRRLVIAMMYGLIKEGGPSYWDQAATLFDWGFAQSPQASIGSL
HHHCCCCCEEHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCHHCCCC
>Mature Secondary Structure
MRKLWTAAAALLLVGACGAPTAAADSTQPAGSIPIPDGPAQTWIIADLDSGQVLAGRDPN
CCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCEEECCCCC
VAHPPASTIKTLLAQVVLDEVSLDATVVADAADTQVECNCVGIKPGRSYTARQLLDALLL
CCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEEEEEEEECCCCCHHHHHHHHHHHH
VSGNDAANTLAHMLGGPEATVAKMNAKAAALGATNTHASTPSGLDGPNGSGSSTAHDLAV
CCCCCHHHHHHHHHCCCCHHHHHHCCCHHEECCCCCCCCCCCCCCCCCCCCCCHHHHHHH
IFRAAMANPVFAQITAEPSAMFPGDNGLQPITNQDELLQRYPGAIGGKTGFTNAARKTFV
HHHHHHCCCEEEEEECCCCEECCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCHHHHHHHH
GAAARGGRRLVIAMMYGLIKEGGPSYWDQAATLFDWGFAQSPQASIGSL
HHHCCCCCEEHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1548223; 7934829; 9384377 [H]