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The map label for this gene is trmD

Identifier: 41409072

GI number: 41409072

Start: 3308461

End: 3309180

Strand: Reverse

Name: trmD

Synonym: MAP2974c

Alternate gene names: 41409072

Gene position: 3309180-3308461 (Counterclockwise)

Preceding gene: 41409073

Following gene: 41409070

Centisome position: 68.52

GC content: 71.11

Gene sequence:

>720_bases
ATGAGAATCGACGTCGTCACCATCTTTCCGGCCTACCTGGACCCGCTGCGGCAGTCGTTGCCGGGCAAGGCGATTCAGTC
CGGCCTGGTCGATCTGCGGGTGCACGACCTGCGGCGCTGGACGCACGACGCGCACCGCTCGGTCGACGACGCCCCCTATG
GCGGCGGCCCGGGGATGGTGATGAAGGCGCCGGTGTGGGGTGAGGCGCTCGACGAAATCGCCTCAGCGGAAACACTTTTG
GTGGTTCCCACGCCGGCCGGCGTGCTGTTCGACCAGGCCACCGCCGCCCGCTGGAGCGCCGAGCGGCATCTGGTGTTCGC
GTGCGGCCGCTACGAGGGCATTGACCAGCGGGTGGTCGAGGACGCCGCCCGGCGGATGCGGGTCGAAGAGGTCTCCATCG
GCGACTACGTGTTGCCGGGCGGGGAGTCGGCGGCGGTGGTGATGATCGAGGCCGTGTTGCGGCTGCTCGACGGGGTGCTC
GGCAATCCCGCGTCGCGCCACGATGATTCGCACTCGCCGGCACTGGACCGGCGGCTGGAGGGGCCCAGCTACACCCGGCC
GCCGAGCTGGCGCGGGCTGGACGTGCCCGAGGTGTTGCTCTCCGGTGACCATGCCCGGATCGCGGCGTGGCGCCGGGAGG
TCTCGCTGCAGCGCACCCGCGAGCGCCGACCGGAGCTGCTAGCCGATCCGGTTGGGCCGCAAGACGATCCGGGCCGTTAG

Upstream 100 bases:

>100_bases
GTTGGTGCCGTTCGTCAGCGCGATCGTCACCGCGGTGTCGCTGGACGACGGGCTCATCGAGATCGATCCGCCCGACGGCC
TGCTGGACCTGTAGGCCGGC

Downstream 100 bases:

>100_bases
AGCCCGTGCGGCGGGGCCTCGATGCGGCCGTCGGGAAAGATCGTCTTGACCGCCCGGGTGATGGTCTCCCGGGCGGTCGC
GTCGTCGGCGGGCTGCAGCG

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MRIDVVTIFPAYLDPLRQSLPGKAIQSGLVDLRVHDLRRWTHDAHRSVDDAPYGGGPGMVMKAPVWGEALDEIASAETLL
VVPTPAGVLFDQATAARWSAERHLVFACGRYEGIDQRVVEDAARRMRVEEVSIGDYVLPGGESAAVVMIEAVLRLLDGVL
GNPASRHDDSHSPALDRRLEGPSYTRPPSWRGLDVPEVLLSGDHARIAAWRREVSLQRTRERRPELLADPVGPQDDPGR

Sequences:

>Translated_239_residues
MRIDVVTIFPAYLDPLRQSLPGKAIQSGLVDLRVHDLRRWTHDAHRSVDDAPYGGGPGMVMKAPVWGEALDEIASAETLL
VVPTPAGVLFDQATAARWSAERHLVFACGRYEGIDQRVVEDAARRMRVEEVSIGDYVLPGGESAAVVMIEAVLRLLDGVL
GNPASRHDDSHSPALDRRLEGPSYTRPPSWRGLDVPEVLLSGDHARIAAWRREVSLQRTRERRPELLADPVGPQDDPGR
>Mature_239_residues
MRIDVVTIFPAYLDPLRQSLPGKAIQSGLVDLRVHDLRRWTHDAHRSVDDAPYGGGPGMVMKAPVWGEALDEIASAETLL
VVPTPAGVLFDQATAARWSAERHLVFACGRYEGIDQRVVEDAARRMRVEEVSIGDYVLPGGESAAVVMIEAVLRLLDGVL
GNPASRHDDSHSPALDRRLEGPSYTRPPSWRGLDVPEVLLSGDHARIAAWRREVSLQRTRERRPELLADPVGPQDDPGR

Specific function: Specifically methylates guanosine-37 in various tRNAs

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family

Homologues:

Organism=Escherichia coli, GI1788959, Length=233, Percent_Identity=42.0600858369099, Blast_Score=166, Evalue=1e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMD_MYCPA (Q73VN9)

Other databases:

- EMBL:   AE016958
- RefSeq:   NP_961908.1
- ProteinModelPortal:   Q73VN9
- SMR:   Q73VN9
- EnsemblBacteria:   EBMYCT00000039602
- GeneID:   2719466
- GenomeReviews:   AE016958_GR
- KEGG:   mpa:MAP2974c
- NMPDR:   fig|262316.1.peg.2974
- GeneTree:   EBGT00050000016420
- HOGENOM:   HBG285805
- OMA:   VCGRFEG
- ProtClustDB:   PRK00026
- BRENDA:   2.1.1.31
- GO:   GO:0005737
- HAMAP:   MF_00605
- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148
- Gene3D:   G3DSA:1.10.1270.20
- PIRSF:   PIRSF000386
- TIGRFAMs:   TIGR00088

Pfam domain/function: PF01746 tRNA_m1G_MT

EC number: =2.1.1.31

Molecular weight: Translated: 26326; Mature: 26326

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: PS00430 TONB_DEPENDENT_REC_1

Important sites: BINDING 109-109

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIDVVTIFPAYLDPLRQSLPGKAIQSGLVDLRVHDLRRWTHDAHRSVDDAPYGGGPGMV
CEEEEEEECHHHHHHHHHHCCHHHHHHCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCE
MKAPVWGEALDEIASAETLLVVPTPAGVLFDQATAARWSAERHLVFACGRYEGIDQRVVE
EECCCCHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHH
DAARRMRVEEVSIGDYVLPGGESAAVVMIEAVLRLLDGVLGNPASRHDDSHSPALDRRLE
HHHHHHHHHEECCCCEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCC
GPSYTRPPSWRGLDVPEVLLSGDHARIAAWRREVSLQRTRERRPELLADPVGPQDDPGR
CCCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCC
>Mature Secondary Structure
MRIDVVTIFPAYLDPLRQSLPGKAIQSGLVDLRVHDLRRWTHDAHRSVDDAPYGGGPGMV
CEEEEEEECHHHHHHHHHHCCHHHHHHCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCE
MKAPVWGEALDEIASAETLLVVPTPAGVLFDQATAARWSAERHLVFACGRYEGIDQRVVE
EECCCCHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHH
DAARRMRVEEVSIGDYVLPGGESAAVVMIEAVLRLLDGVLGNPASRHDDSHSPALDRRLE
HHHHHHHHHEECCCCEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCC
GPSYTRPPSWRGLDVPEVLLSGDHARIAAWRREVSLQRTRERRPELLADPVGPQDDPGR
CCCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA