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The map label for this gene is ispG

Identifier: 41409036

GI number: 41409036

Start: 3272755

End: 3273921

Strand: Reverse

Name: ispG

Synonym: MAP2938c

Alternate gene names: 41409036

Gene position: 3273921-3272755 (Counterclockwise)

Preceding gene: 41409037

Following gene: 41409035

Centisome position: 67.79

GC content: 68.98

Gene sequence:

>1167_bases
GTGACGACAGGCCTGGGCATGCCGCAGACCCCGGCGCCCACGCTTGCGCCCCGGCGCCGCACGCGCCAACTGATGGTGCG
CGACGTCGGGGTCGGCAGCGACTATCCGATCTCGGTGCAGTCGATGTGCACCACCAAGACCCACGACGTGAACTCGACGC
TGCAGCAGATCGCCGAGCTGACCGCGGCCGGCTGCGACATCGTCCGGGTGGCCTGCCCGCGCCAGGAGGACGCCGACGCG
CTGGCCGAGATCGCCCGGCACAGCCAGATCCCGGTGATCGCCGACATCCACTTCCAGCCCAAGTACATCTTCGCGGCCAT
CGACGCCGGCTGCGCCGCGGTGCGGGTGAACCCCGGCAACATCAAGGAATTCGACGGCCGGGTGGGCGAGGTCGCCAAAG
CCGCTGCGGCAGCGGGCATTCCGATCCGCATCGGCGTCAACGCGGGCTCGCTGGACAAGCGGTTCATGGCCAAGTACGGC
AAGGCCACCCCCGAGGCGCTGGTCGAGTCCGCGCTGTGGGAGGCCTCGCTGTTCGAGGAGCACGGCTTTTCCGACATCAA
GATCAGCGTCAAGCACAACGACCCCGTGGTGATGGTCGCCGCCTACGAGCAACTCGCCGAGCAGTGCGACTACCCGCTGC
ACCTGGGCGTCACCGAGGCCGGGCCCGCGTTTCAGGGCACCATCAAGTCCGCGGTCGCGTTCGGTGCGCTGCTGTCGCGC
GGTATCGGCGACACCATCCGGGTGTCGCTGTCGGCGCCGCCGGTCGAGGAGGTCAAGGTCGGCATCCAGATCCTGGAGTC
GCTGAACCTGCGGCCGCGTTCGCTCGAGATCGTGTCCTGCCCGTCGTGCGGGCGCGCGCAGGTCGACGTCTACACGCTGG
CCAACGAGGTCAGCGCCGGCCTGGACGGCCTGGACGTGCCGCTGCGGGTCGCGGTGATGGGCTGCGTGGTCAACGGGCCG
GGGGAGGCCCGCGAGGCCGACCTGGGCGTCGCGTCGGGAAATGGCAAGGGGCAGATCTTCGTTCGCGGCGAGGTGATCAA
GACGGTGCCCGAATCCCAGATCGTGGAGACGCTGATCGAAGAGGCGATGCGCATCGCCGCGCAGATGAACAGTGAGGGCG
GGCCGGAGGCAACATCCAGCGGTTCACCGGTTGTCACCGTAAGCTGA

Upstream 100 bases:

>100_bases
TCGTGGTGGGCTACATGTTGCTGACGGTGACCGCGGACCTGGTCAACCCGATCAGGCTCTTCCAGTAATCGACTCCGGTA
ATCGACGTGAAGGAACGAAC

Downstream 100 bases:

>100_bases
TAAGGGCCAGCGTCGGTCCACGAGTTTGCAGCACAGAGAGTTCGCAGCATGTCGGCTCCACCCCTGTTCCGCCTTGTCGG
CGAGCGACGGGTGTCCGTGG

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase

Number of amino acids: Translated: 388; Mature: 387

Protein sequence:

>388_residues
MTTGLGMPQTPAPTLAPRRRTRQLMVRDVGVGSDYPISVQSMCTTKTHDVNSTLQQIAELTAAGCDIVRVACPRQEDADA
LAEIARHSQIPVIADIHFQPKYIFAAIDAGCAAVRVNPGNIKEFDGRVGEVAKAAAAAGIPIRIGVNAGSLDKRFMAKYG
KATPEALVESALWEASLFEEHGFSDIKISVKHNDPVVMVAAYEQLAEQCDYPLHLGVTEAGPAFQGTIKSAVAFGALLSR
GIGDTIRVSLSAPPVEEVKVGIQILESLNLRPRSLEIVSCPSCGRAQVDVYTLANEVSAGLDGLDVPLRVAVMGCVVNGP
GEAREADLGVASGNGKGQIFVRGEVIKTVPESQIVETLIEEAMRIAAQMNSEGGPEATSSGSPVVTVS

Sequences:

>Translated_388_residues
MTTGLGMPQTPAPTLAPRRRTRQLMVRDVGVGSDYPISVQSMCTTKTHDVNSTLQQIAELTAAGCDIVRVACPRQEDADA
LAEIARHSQIPVIADIHFQPKYIFAAIDAGCAAVRVNPGNIKEFDGRVGEVAKAAAAAGIPIRIGVNAGSLDKRFMAKYG
KATPEALVESALWEASLFEEHGFSDIKISVKHNDPVVMVAAYEQLAEQCDYPLHLGVTEAGPAFQGTIKSAVAFGALLSR
GIGDTIRVSLSAPPVEEVKVGIQILESLNLRPRSLEIVSCPSCGRAQVDVYTLANEVSAGLDGLDVPLRVAVMGCVVNGP
GEAREADLGVASGNGKGQIFVRGEVIKTVPESQIVETLIEEAMRIAAQMNSEGGPEATSSGSPVVTVS
>Mature_387_residues
TTGLGMPQTPAPTLAPRRRTRQLMVRDVGVGSDYPISVQSMCTTKTHDVNSTLQQIAELTAAGCDIVRVACPRQEDADAL
AEIARHSQIPVIADIHFQPKYIFAAIDAGCAAVRVNPGNIKEFDGRVGEVAKAAAAAGIPIRIGVNAGSLDKRFMAKYGK
ATPEALVESALWEASLFEEHGFSDIKISVKHNDPVVMVAAYEQLAEQCDYPLHLGVTEAGPAFQGTIKSAVAFGALLSRG
IGDTIRVSLSAPPVEEVKVGIQILESLNLRPRSLEIVSCPSCGRAQVDVYTLANEVSAGLDGLDVPLRVAVMGCVVNGPG
EAREADLGVASGNGKGQIFVRGEVIKTVPESQIVETLIEEAMRIAAQMNSEGGPEATSSGSPVVTVS

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family

Homologues:

Organism=Escherichia coli, GI1788863, Length=361, Percent_Identity=42.6592797783933, Blast_Score=293, Evalue=2e-80,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPG_MYCPA (Q73VS3)

Other databases:

- EMBL:   AE016958
- RefSeq:   NP_961872.1
- ProteinModelPortal:   Q73VS3
- EnsemblBacteria:   EBMYCT00000039086
- GeneID:   2719134
- GenomeReviews:   AE016958_GR
- KEGG:   mpa:MAP2938c
- NMPDR:   fig|262316.1.peg.2938
- GeneTree:   EBGT00050000017148
- HOGENOM:   HBG335271
- OMA:   APIVVQS
- ProtClustDB:   PRK00366
- BRENDA:   1.17.4.3
- HAMAP:   MF_00159
- InterPro:   IPR013785
- InterPro:   IPR011005
- InterPro:   IPR016425
- InterPro:   IPR004588
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF004640
- TIGRFAMs:   TIGR00612

Pfam domain/function: PF04551 GcpE; SSF51717 DHP_synth_like

EC number: =1.17.7.1

Molecular weight: Translated: 40808; Mature: 40677

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTGLGMPQTPAPTLAPRRRTRQLMVRDVGVGSDYPISVQSMCTTKTHDVNSTLQQIAEL
CCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCEEHHHHHCCCCCCHHHHHHHHHHH
TAAGCDIVRVACPRQEDADALAEIARHSQIPVIADIHFQPKYIFAAIDAGCAAVRVNPGN
HHCCCCEEEEECCCCCCHHHHHHHHHCCCCCEEEEEECCCCEEEEEECCCCEEEEECCCC
IKEFDGRVGEVAKAAAAAGIPIRIGVNAGSLDKRFMAKYGKATPEALVESALWEASLFEE
CHHHCCHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
HGFSDIKISVKHNDPVVMVAAYEQLAEQCDYPLHLGVTEAGPAFQGTIKSAVAFGALLSR
CCCCEEEEEEECCCCEEEEEHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHC
GIGDTIRVSLSAPPVEEVKVGIQILESLNLRPRSLEIVSCPSCGRAQVDVYTLANEVSAG
CCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCEEEEEEHHHHHHCC
LDGLDVPLRVAVMGCVVNGPGEAREADLGVASGNGKGQIFVRGEVIKTVPESQIVETLIE
CCCCCCHHHEEEEEEEECCCCCCCCCCCEEECCCCCEEEEEECHHHHHCCHHHHHHHHHH
EAMRIAAQMNSEGGPEATSSGSPVVTVS
HHHHHHHHHCCCCCCCCCCCCCCEEEEC
>Mature Secondary Structure 
TTGLGMPQTPAPTLAPRRRTRQLMVRDVGVGSDYPISVQSMCTTKTHDVNSTLQQIAEL
CCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCEEHHHHHCCCCCCHHHHHHHHHHH
TAAGCDIVRVACPRQEDADALAEIARHSQIPVIADIHFQPKYIFAAIDAGCAAVRVNPGN
HHCCCCEEEEECCCCCCHHHHHHHHHCCCCCEEEEEECCCCEEEEEECCCCEEEEECCCC
IKEFDGRVGEVAKAAAAAGIPIRIGVNAGSLDKRFMAKYGKATPEALVESALWEASLFEE
CHHHCCHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
HGFSDIKISVKHNDPVVMVAAYEQLAEQCDYPLHLGVTEAGPAFQGTIKSAVAFGALLSR
CCCCEEEEEEECCCCEEEEEHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHC
GIGDTIRVSLSAPPVEEVKVGIQILESLNLRPRSLEIVSCPSCGRAQVDVYTLANEVSAG
CCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCEEEEEEHHHHHHCC
LDGLDVPLRVAVMGCVVNGPGEAREADLGVASGNGKGQIFVRGEVIKTVPESQIVETLIE
CCCCCCHHHEEEEEEEECCCCCCCCCCCEEECCCCCEEEEEECHHHHHCCHHHHHHHHHH
EAMRIAAQMNSEGGPEATSSGSPVVTVS
HHHHHHHHHCCCCCCCCCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA