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The map label for this gene is cobB

Identifier: 41409015

GI number: 41409015

Start: 3249300

End: 3250712

Strand: Reverse

Name: cobB

Synonym: MAP2917c

Alternate gene names: 41409015

Gene position: 3250712-3249300 (Counterclockwise)

Preceding gene: 41409016

Following gene: 41409014

Centisome position: 67.31

GC content: 74.95

Gene sequence:

>1413_bases
GTGGTGAACGTGCCCGCGGTGGTCGTCGCGGCGCCCGCCTCCGGCAGCGGAAAGACCACGGTGGCAACGGGTTTGATCGG
GGCCCTGCGCGGGGCGGGCCGCCGGGTGGCGGCGTTCAAGGTGGGGCCGGACTTCATCGATCCCGGCTATCACGGCCTGA
CCGCCGGCCGGCCGGGCCGCAACCTCGACCCGGTGTTGGTGGGGGAGAACCTGATTGGCCCGCTGTACGCGCACGGCGCC
GCCGGCGCCGACATCGCCGTCGTCGAGGGCGTGATGGGGCTGTTCGACGGGCGGATCGGGCCGTCCGGCGCCAGCCCGGC
GGCGGGATCCACCGCGCACGTCGCCGCGCTGCTGGGCGCGCCCGTCGTGCTGGTCGTCGACGCGCGCGGCCAGAGCCAGA
GCATCGCGGCCCTGCTGCACGGCTTCTCGACGTTCGACGCCGCGACCCGGGTCGCCGGGGTGATCCTGAACCGGGTCGGG
TCGGCCCGTCACGAGCAGGTGCTGCGTCAGGCGTCCGAACAGGCCGGCGTGCCGGTGCTGGGCGCCATCCCGCGCAGCGC
CGAATTAGAGTTGCCAACAAGGTACTTGGGCTTGGTCACGGCGGTCGAGTATGGGCGCCGCGCCCGGCTCGCGGTCGAGG
CGATGACCGCGCTGGTGGCACGCCACGTCGACCTGGCGGCGGTGGCGGCGGTCGCCGCCGCCCGGCCCGTCACGACGCCC
TGGGATCCGGCGGCGGCGGTGGGGGATCCCGGCGGGTGCGGCGGTGAGCGGGCCACCGTCGCGGTGGCCACCGGGAAGGC
GTTCAGCTTCGGCTACGCCGAGCACGCGGAGCTGCTGGCCGCCGCCGGCGCCCGGGTGGCCGAATTCGACCCGCTCGTCG
ACGCGTTGCCCGACGGCACCGACGCGCTGTTGCTGCCCGGCGGTTTTCCCGAACAGTTCACCGCCGAGCTGTCGGCCAAC
GAGGTCGTGCGCCGCCAGATCAACGAGCTGGCCGCCGCCGGGGCGCCCGTCCACGCCGAGTGCGCCGGGCTGCTCTACTT
GGTGTCCGAACTCGACGGCCACCCCATGTGCGGGGTGCTGGCCGGGCAGGCCCGGTTCACCCCGCAGCTGACGCTGGCCT
ATCGCGATGCCGTGGCCGTCGTCGACTCCCCGCTGTACGCCGCCGGCCAGCGGGCGGTGGGCCACGAATTCCACCGCACC
ACAGTGACATTCAGCGACGACTACCCGCCGGCGTGGATGTACCGAACCCAGGAGTCCGGCCATCGCGACGGCGCGGTGCG
CGGCGGTGTGCACGCGTCGTATCTGCACACCCACCCCGCCGCCGCGCCCGCGGCGGTGGCCCGCTTCGTCGGGTGCGCGC
TGGCCGCTCGCGCCGACCCGGGCAGGACGCGGGCCCACCCCGGCAACCACTAG

Upstream 100 bases:

>100_bases
ACGCCCCGCAACGGCTCATCGATGCGGCCGACCTGGTCACCGAGATGGCCAAGGTGAAGCACCCGATGGACGCCGGGCGC
AAGGGGCAGAAGGGCATCGA

Downstream 100 bases:

>100_bases
GCTTGCCGGGTGACCGAGAGCGCCTACCTGGTGGGGCTGCGGCTGACCGGCAAGAAGGTGGTCGTGGTCGGCGGCGGCAC
CGTCGCCCAGCGCCGGCTGC

Product: cobyrinic acid a,c-diamide synthase

Products: ADP; phosphate; Hydrogenobyrinic acid a,c diamide; L-glutamate

Alternate protein names: NA

Number of amino acids: Translated: 470; Mature: 470

Protein sequence:

>470_residues
MVNVPAVVVAAPASGSGKTTVATGLIGALRGAGRRVAAFKVGPDFIDPGYHGLTAGRPGRNLDPVLVGENLIGPLYAHGA
AGADIAVVEGVMGLFDGRIGPSGASPAAGSTAHVAALLGAPVVLVVDARGQSQSIAALLHGFSTFDAATRVAGVILNRVG
SARHEQVLRQASEQAGVPVLGAIPRSAELELPTRYLGLVTAVEYGRRARLAVEAMTALVARHVDLAAVAAVAAARPVTTP
WDPAAAVGDPGGCGGERATVAVATGKAFSFGYAEHAELLAAAGARVAEFDPLVDALPDGTDALLLPGGFPEQFTAELSAN
EVVRRQINELAAAGAPVHAECAGLLYLVSELDGHPMCGVLAGQARFTPQLTLAYRDAVAVVDSPLYAAGQRAVGHEFHRT
TVTFSDDYPPAWMYRTQESGHRDGAVRGGVHASYLHTHPAAAPAAVARFVGCALAARADPGRTRAHPGNH

Sequences:

>Translated_470_residues
MVNVPAVVVAAPASGSGKTTVATGLIGALRGAGRRVAAFKVGPDFIDPGYHGLTAGRPGRNLDPVLVGENLIGPLYAHGA
AGADIAVVEGVMGLFDGRIGPSGASPAAGSTAHVAALLGAPVVLVVDARGQSQSIAALLHGFSTFDAATRVAGVILNRVG
SARHEQVLRQASEQAGVPVLGAIPRSAELELPTRYLGLVTAVEYGRRARLAVEAMTALVARHVDLAAVAAVAAARPVTTP
WDPAAAVGDPGGCGGERATVAVATGKAFSFGYAEHAELLAAAGARVAEFDPLVDALPDGTDALLLPGGFPEQFTAELSAN
EVVRRQINELAAAGAPVHAECAGLLYLVSELDGHPMCGVLAGQARFTPQLTLAYRDAVAVVDSPLYAAGQRAVGHEFHRT
TVTFSDDYPPAWMYRTQESGHRDGAVRGGVHASYLHTHPAAAPAAVARFVGCALAARADPGRTRAHPGNH
>Mature_470_residues
MVNVPAVVVAAPASGSGKTTVATGLIGALRGAGRRVAAFKVGPDFIDPGYHGLTAGRPGRNLDPVLVGENLIGPLYAHGA
AGADIAVVEGVMGLFDGRIGPSGASPAAGSTAHVAALLGAPVVLVVDARGQSQSIAALLHGFSTFDAATRVAGVILNRVG
SARHEQVLRQASEQAGVPVLGAIPRSAELELPTRYLGLVTAVEYGRRARLAVEAMTALVARHVDLAAVAAVAAARPVTTP
WDPAAAVGDPGGCGGERATVAVATGKAFSFGYAEHAELLAAAGARVAEFDPLVDALPDGTDALLLPGGFPEQFTAELSAN
EVVRRQINELAAAGAPVHAECAGLLYLVSELDGHPMCGVLAGQARFTPQLTLAYRDAVAVVDSPLYAAGQRAVGHEFHRT
TVTFSDDYPPAWMYRTQESGHRDGAVRGGVHASYLHTHPAAAPAAVARFVGCALAARADPGRTRAHPGNH

Specific function: Responsible for the amidation of carboxylic groups at position A and C of either cobyrinic acid or hydrogenobrynic acid. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation [H]

COG id: COG1797

COG function: function code H; Cobyrinic acid a,c-diamide synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GATase cobBQ-type domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002586
- InterPro:   IPR004484
- InterPro:   IPR017929
- InterPro:   IPR011698 [H]

Pfam domain/function: PF01656 CbiA; PF07685 GATase_3 [H]

EC number: 6.3.5.9

Molecular weight: Translated: 47901; Mature: 47901

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVNVPAVVVAAPASGSGKTTVATGLIGALRGAGRRVAAFKVGPDFIDPGYHGLTAGRPGR
CCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCC
NLDPVLVGENLIGPLYAHGAAGADIAVVEGVMGLFDGRIGPSGASPAAGSTAHVAALLGA
CCCCEEECCCCCCHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCC
PVVLVVDARGQSQSIAALLHGFSTFDAATRVAGVILNRVGSARHEQVLRQASEQAGVPVL
CEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEE
GAIPRSAELELPTRYLGLVTAVEYGRRARLAVEAMTALVARHVDLAAVAAVAAARPVTTP
ECCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
WDPAAAVGDPGGCGGERATVAVATGKAFSFGYAEHAELLAAAGARVAEFDPLVDALPDGT
CCCCCCCCCCCCCCCCCEEEEEEECCEEECCCHHHHHHHHHCCCCHHHHCHHHHHCCCCC
DALLLPGGFPEQFTAELSANEVVRRQINELAAAGAPVHAECAGLLYLVSELDGHPMCGVL
CEEEECCCCCHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCEEEE
AGQARFTPQLTLAYRDAVAVVDSPLYAAGQRAVGHEFHRTTVTFSDDYPPAWMYRTQESG
ECCCCCCCEEEEEEHHHHHHHCCCHHHHCCHHHCCHHHEEEEEECCCCCCCEEEECCCCC
HRDGAVRGGVHASYLHTHPAAAPAAVARFVGCALAARADPGRTRAHPGNH
CCCCCCCCCCEEHHEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MVNVPAVVVAAPASGSGKTTVATGLIGALRGAGRRVAAFKVGPDFIDPGYHGLTAGRPGR
CCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCC
NLDPVLVGENLIGPLYAHGAAGADIAVVEGVMGLFDGRIGPSGASPAAGSTAHVAALLGA
CCCCEEECCCCCCHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCC
PVVLVVDARGQSQSIAALLHGFSTFDAATRVAGVILNRVGSARHEQVLRQASEQAGVPVL
CEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEE
GAIPRSAELELPTRYLGLVTAVEYGRRARLAVEAMTALVARHVDLAAVAAVAAARPVTTP
ECCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
WDPAAAVGDPGGCGGERATVAVATGKAFSFGYAEHAELLAAAGARVAEFDPLVDALPDGT
CCCCCCCCCCCCCCCCCEEEEEEECCEEECCCHHHHHHHHHCCCCHHHHCHHHHHCCCCC
DALLLPGGFPEQFTAELSANEVVRRQINELAAAGAPVHAECAGLLYLVSELDGHPMCGVL
CEEEECCCCCHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCEEEE
AGQARFTPQLTLAYRDAVAVVDSPLYAAGQRAVGHEFHRTTVTFSDDYPPAWMYRTQESG
ECCCCCCCEEEEEEHHHHHHHCCCHHHHCCHHHCCHHHEEEEEECCCCCCCEEEECCCCC
HRDGAVRGGVHASYLHTHPAAAPAAVARFVGCALAARADPGRTRAHPGNH
CCCCCCCCCCEEHHEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; hydrogenobyrinic acid; L-glutamine; H2O

Specific reaction: 2 ATP + hydrogenobyrinic acid + 2 L-glutamine + 2 H2O = 2 ADP + 2 phosphate + hydrogenobyrinic acid a,c-diamide + 2 L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]