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The map label for this gene is proS

Identifier: 41409011

GI number: 41409011

Start: 3243196

End: 3244944

Strand: Reverse

Name: proS

Synonym: MAP2913c

Alternate gene names: 41409011

Gene position: 3244944-3243196 (Counterclockwise)

Preceding gene: 41409012

Following gene: 41409008

Centisome position: 67.19

GC content: 70.5

Gene sequence:

>1749_bases
GTGATCACCCGGATGTCCCAGCTGTTCCTGCGCACCCTGCGCGACGACCCCGCCGACGCCGAAGTGCCCAGCCACAAGCT
GCTGATCCGGGCCGGATACATCCGGCCCGTCGCGCCCGGGCTGTACAGCTGGCTCCCGCTGGGGCTGCGGGTGCTGCGCC
GGATCGAGCACATCGTCCGCGAGGAGATGAATGCGATTGGCGGCCAAGAGATCCTGTTTCCCGCCCTGCTGCCGCGCGCG
CCGTACGAGGCGACGAACCGGTGGACCGAGTACGGCGACAGCGTTTTTCGGCTGCAGGACCGCCGCGGCAACGACTACCT
GCTGGGGCCCACCCACGAGGAGCTGTTCACCCTGACCGTCAAGGGTGAATACAGCTCCTACAAAGACTTTCCGGTGCTGC
TGTATCAGATCCAGAACAAATACCGCGACGAGGCGCGGCCACGGGCAGGCATCCTGCGGGTGCGCGAGTTCGTGATGAAG
GACTCCTACTCGTTCGACATCGACGACGCCGGGCTCAAGGCCGCCTATCACGCACACCGGGAGGCCTATCAGCGCATCTT
CGCGCGGCTGCAGGTGCGCTACGTGATCGTGTCCGCGGTGTCGGGCGCCATGGGCGGCAGCGCCTCCGAGGAGTTCCTGG
CCGAGAGCCCGGTCGGCGAGGACACCTTCGTGCGCTGCCTGGAGTCCGGCTATGCGGCCAACGTCGAGGCGGTGATCACC
GCCCGCCCGGACCCGCAACCCGTCGAGGGTCTGCCCGAGGCGGTGGTCCACGACACCGGTGACACCCCGACCATCGCCAC
CCTGGTCGACTGGGCCAACCGGGCGGGCCTGGGCCGCGCCGTCACCGCGGCCGACACGTTGAAGAACGTCCTGCTCAAGG
TGCGCCAGCCCGGCGGGGACTGGGAGCTGCTGGCCATCGGCCTGCCCGGCGACCGTGAGGTCGACGACAAGCGGCTGGGC
GCGGCGCTGGAGCCGGCCGAGTACGTGCTGCTCGACGACGCCGACTTCGCCAGGTACCCGTTCCTGGTGAAGGGTTACAT
CGGTCCGAAAGCGTTGAAGGACAACGGAGTTCGTTACCTGGTCGATCCGCGGGTGGTGGACGGCACCAGCTGGATCACCG
GGGCCGACGAACCGGGCCGCCATGTCGTCGGGCTGGTCGCGGGCCGGGACTTCACCGCCGACGGCACCATCGAGGCCGCC
GAGGTGCGCGACGGCGACCCGTCGCCGGACGGCGCCGGGCCGCTGGTGTCCGCGCGCGGCATCGAGGTCGCCCACATCTT
CCAGTTGGGCCGCAAATACACCGACGCCTTCACCGCCGACGTGCTGGGCGAGGACGGCAAGCCGGTGCGGCTCACCATGG
GCTCCTACGGCCTGGGGGTGTCGCGGATGGTGGCCGTCATCGCCGAACAGCACCACGACGAGCTGGGCCTGCGCTGGCCC
GCGTCGGTGGCGCCGTTCGACGTCCACCTGGTGATCGCCAACAAGGACGCGCAGGCGCGCGCCGGAGCCACCGCGCTGGC
CGACGACCTGGACCGGCTCGGCGTCGAGGTGCTGCTCGACGACCGTCAGGCCTCGCCGGGAGTGAAGTTCAAGGACGCCG
AGCTGCTGGGCGTGCCGTGGATCGTGGTGGTGGGCCGGGGCTGGGCCGACGGTGTGGTGGAGCTGCGCGACCGCTTCGCC
GGCCAGACCCGGGAGCTGGCCACCGGGCCGTCGCTTGCCGCCGACATCGCGGCCGCCCTGCGCGGCTAG

Upstream 100 bases:

>100_bases
GTTCACCACGCAACGGGCGCCCTACTGCGGCACGCTGTTCTGACGCCGGCGCACACGCGCCGGTGAGGGCGACCCGGTCG
CCAGGCTAGGCTAGCCCGCT

Downstream 100 bases:

>100_bases
TCAGTCGTTGCCGCCCGGGAAGCTGGTGGTGATCGGCCAGGCGCCCAGCACCCGGTTCCACCGGGCCGCCATCACCGCGC
TTTGGACCAGGGCGGTGGCG

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS [H]

Number of amino acids: Translated: 582; Mature: 582

Protein sequence:

>582_residues
MITRMSQLFLRTLRDDPADAEVPSHKLLIRAGYIRPVAPGLYSWLPLGLRVLRRIEHIVREEMNAIGGQEILFPALLPRA
PYEATNRWTEYGDSVFRLQDRRGNDYLLGPTHEELFTLTVKGEYSSYKDFPVLLYQIQNKYRDEARPRAGILRVREFVMK
DSYSFDIDDAGLKAAYHAHREAYQRIFARLQVRYVIVSAVSGAMGGSASEEFLAESPVGEDTFVRCLESGYAANVEAVIT
ARPDPQPVEGLPEAVVHDTGDTPTIATLVDWANRAGLGRAVTAADTLKNVLLKVRQPGGDWELLAIGLPGDREVDDKRLG
AALEPAEYVLLDDADFARYPFLVKGYIGPKALKDNGVRYLVDPRVVDGTSWITGADEPGRHVVGLVAGRDFTADGTIEAA
EVRDGDPSPDGAGPLVSARGIEVAHIFQLGRKYTDAFTADVLGEDGKPVRLTMGSYGLGVSRMVAVIAEQHHDELGLRWP
ASVAPFDVHLVIANKDAQARAGATALADDLDRLGVEVLLDDRQASPGVKFKDAELLGVPWIVVVGRGWADGVVELRDRFA
GQTRELATGPSLAADIAAALRG

Sequences:

>Translated_582_residues
MITRMSQLFLRTLRDDPADAEVPSHKLLIRAGYIRPVAPGLYSWLPLGLRVLRRIEHIVREEMNAIGGQEILFPALLPRA
PYEATNRWTEYGDSVFRLQDRRGNDYLLGPTHEELFTLTVKGEYSSYKDFPVLLYQIQNKYRDEARPRAGILRVREFVMK
DSYSFDIDDAGLKAAYHAHREAYQRIFARLQVRYVIVSAVSGAMGGSASEEFLAESPVGEDTFVRCLESGYAANVEAVIT
ARPDPQPVEGLPEAVVHDTGDTPTIATLVDWANRAGLGRAVTAADTLKNVLLKVRQPGGDWELLAIGLPGDREVDDKRLG
AALEPAEYVLLDDADFARYPFLVKGYIGPKALKDNGVRYLVDPRVVDGTSWITGADEPGRHVVGLVAGRDFTADGTIEAA
EVRDGDPSPDGAGPLVSARGIEVAHIFQLGRKYTDAFTADVLGEDGKPVRLTMGSYGLGVSRMVAVIAEQHHDELGLRWP
ASVAPFDVHLVIANKDAQARAGATALADDLDRLGVEVLLDDRQASPGVKFKDAELLGVPWIVVVGRGWADGVVELRDRFA
GQTRELATGPSLAADIAAALRG
>Mature_582_residues
MITRMSQLFLRTLRDDPADAEVPSHKLLIRAGYIRPVAPGLYSWLPLGLRVLRRIEHIVREEMNAIGGQEILFPALLPRA
PYEATNRWTEYGDSVFRLQDRRGNDYLLGPTHEELFTLTVKGEYSSYKDFPVLLYQIQNKYRDEARPRAGILRVREFVMK
DSYSFDIDDAGLKAAYHAHREAYQRIFARLQVRYVIVSAVSGAMGGSASEEFLAESPVGEDTFVRCLESGYAANVEAVIT
ARPDPQPVEGLPEAVVHDTGDTPTIATLVDWANRAGLGRAVTAADTLKNVLLKVRQPGGDWELLAIGLPGDREVDDKRLG
AALEPAEYVLLDDADFARYPFLVKGYIGPKALKDNGVRYLVDPRVVDGTSWITGADEPGRHVVGLVAGRDFTADGTIEAA
EVRDGDPSPDGAGPLVSARGIEVAHIFQLGRKYTDAFTADVLGEDGKPVRLTMGSYGLGVSRMVAVIAEQHHDELGLRWP
ASVAPFDVHLVIANKDAQARAGATALADDLDRLGVEVLLDDRQASPGVKFKDAELLGVPWIVVVGRGWADGVVELRDRFA
GQTRELATGPSLAADIAAALRG

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI34303926, Length=240, Percent_Identity=38.75, Blast_Score=191, Evalue=2e-48,
Organism=Escherichia coli, GI1786392, Length=575, Percent_Identity=40.695652173913, Blast_Score=402, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI115532348, Length=247, Percent_Identity=34.0080971659919, Blast_Score=138, Evalue=8e-33,
Organism=Saccharomyces cerevisiae, GI6320931, Length=568, Percent_Identity=29.0492957746479, Blast_Score=223, Evalue=7e-59,
Organism=Drosophila melanogaster, GI24656200, Length=216, Percent_Identity=37.5, Blast_Score=160, Evalue=2e-39,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK [H]

EC number: =6.1.1.15 [H]

Molecular weight: Translated: 63554; Mature: 63554

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: PS50862 AA_TRNA_LIGASE_II ; PS00430 TONB_DEPENDENT_REC_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITRMSQLFLRTLRDDPADAEVPSHKLLIRAGYIRPVAPGLYSWLPLGLRVLRRIEHIVR
CCCHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHH
EEMNAIGGQEILFPALLPRAPYEATNRWTEYGDSVFRLQDRRGNDYLLGPTHEELFTLTV
HHHHHCCCCHHHHHHHCCCCCCHHHHHHHHHCCCEEEEECCCCCEEEECCCCCCEEEEEE
KGEYSSYKDFPVLLYQIQNKYRDEARPRAGILRVREFVMKDSYSFDIDDAGLKAAYHAHR
ECCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
EAYQRIFARLQVRYVIVSAVSGAMGGSASEEFLAESPVGEDTFVRCLESGYAANVEAVIT
HHHHHHHHHHHHHHHEEEHHHHCCCCCCCHHHHHCCCCCHHHHHHHHHCCCCCCEEEEEE
ARPDPQPVEGLPEAVVHDTGDTPTIATLVDWANRAGLGRAVTAADTLKNVLLKVRQPGGD
ECCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCC
WELLAIGLPGDREVDDKRLGAALEPAEYVLLDDADFARYPFLVKGYIGPKALKDNGVRYL
EEEEEEECCCCCCCCHHHCCCCCCCCCEEEEECCCCCCCCEEEEECCCCCHHCCCCCEEE
VDPRVVDGTSWITGADEPGRHVVGLVAGRDFTADGTIEAAEVRDGDPSPDGAGPLVSARG
ECCEEECCCCCCCCCCCCCCEEEEEEECCCCCCCCCEEEEECCCCCCCCCCCCCCEECCC
IEVAHIFQLGRKYTDAFTADVLGEDGKPVRLTMGSYGLGVSRMVAVIAEQHHDELGLRWP
CHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCC
ASVAPFDVHLVIANKDAQARAGATALADDLDRLGVEVLLDDRQASPGVKFKDAELLGVPW
CCCCCEEEEEEEECCCCHHHCCCHHHHHHHHHCCEEEEEECCCCCCCCEECCCEEECCCE
IVVVGRGWADGVVELRDRFAGQTRELATGPSLAADIAAALRG
EEEECCCCHHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MITRMSQLFLRTLRDDPADAEVPSHKLLIRAGYIRPVAPGLYSWLPLGLRVLRRIEHIVR
CCCHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHH
EEMNAIGGQEILFPALLPRAPYEATNRWTEYGDSVFRLQDRRGNDYLLGPTHEELFTLTV
HHHHHCCCCHHHHHHHCCCCCCHHHHHHHHHCCCEEEEECCCCCEEEECCCCCCEEEEEE
KGEYSSYKDFPVLLYQIQNKYRDEARPRAGILRVREFVMKDSYSFDIDDAGLKAAYHAHR
ECCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
EAYQRIFARLQVRYVIVSAVSGAMGGSASEEFLAESPVGEDTFVRCLESGYAANVEAVIT
HHHHHHHHHHHHHHHEEEHHHHCCCCCCCHHHHHCCCCCHHHHHHHHHCCCCCCEEEEEE
ARPDPQPVEGLPEAVVHDTGDTPTIATLVDWANRAGLGRAVTAADTLKNVLLKVRQPGGD
ECCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCC
WELLAIGLPGDREVDDKRLGAALEPAEYVLLDDADFARYPFLVKGYIGPKALKDNGVRYL
EEEEEEECCCCCCCCHHHCCCCCCCCCEEEEECCCCCCCCEEEEECCCCCHHCCCCCEEE
VDPRVVDGTSWITGADEPGRHVVGLVAGRDFTADGTIEAAEVRDGDPSPDGAGPLVSARG
ECCEEECCCCCCCCCCCCCCEEEEEEECCCCCCCCCEEEEECCCCCCCCCCCCCCEECCC
IEVAHIFQLGRKYTDAFTADVLGEDGKPVRLTMGSYGLGVSRMVAVIAEQHHDELGLRWP
CHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCC
ASVAPFDVHLVIANKDAQARAGATALADDLDRLGVEVLLDDRQASPGVKFKDAELLGVPW
CCCCCEEEEEEEECCCCHHHCCCHHHHHHHHHCCEEEEEECCCCCCCCEECCCEEECCCE
IVVVGRGWADGVVELRDRFAGQTRELATGPSLAADIAAALRG
EEEECCCCHHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA