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The map label for this gene is ispH

Identifier: 41408782

GI number: 41408782

Start: 3019437

End: 3020435

Strand: Reverse

Name: ispH

Synonym: MAP2684c

Alternate gene names: 41408782

Gene position: 3020435-3019437 (Counterclockwise)

Preceding gene: 41408787

Following gene: 41408780

Centisome position: 62.54

GC content: 70.47

Gene sequence:

>999_bases
ATGCCGCCGACCGTCGACATGGGGATTCCCGGCGCATCCAGCTCCGTAGCCGTCAACCCGACCCGCAAGCGCGTGCTCCT
GGCCGAGCCGCGCGGATACTGCGCGGGCGTGGACCGGGCCGTGGAAACGGTGGAGCGCGCACTGGAGAAGCACGGCCCGC
CGGTGTACGTGCGCCACGAGATCGTGCACAACCGGCACGTGGTCAGCACCCTGGAGAAGGCCGGCGCGGTGTTCGTCGAG
GAGACCGACGAGGTTCCCGAAGGGGCGATCGTGGTGTTCTCCGCGCACGGCGTGGCGCCGACGGTGCACGCCGCGGCCGC
CGAACGCAACCTGCACACCATCGACGCCACCTGCCCGCTGGTGACCAAGGTGCACAACGAGGCGCGCCGGTTCGCCCGCA
ACGACTACGACATCCTGCTGATCGGCCACGAGGGCCACGAGGAGGTGGTCGGCACCGCTGGGGAGGCGCCCGACCATGTG
CAGCTGGTCGACGGGGTGGCCGCGGTGGACAACGTGAGGGTCCGCGACGAGGACAAGGTGGTGTGGCTGTCGCAGACCAC
GCTGTCGGTCGACGAGACCATGGAGATCGTCGAGCGGCTCCGGCAGCGGTTCCCCAAGCTGCAGGACCCGCCGAGTGACG
ACATCTGCTACGCCACCCAGAACCGCCAGGTGGCGGTCAAGGCGATGGCGCCGGAATGCGACCTGGTGATCGTCGTCGGC
TCGCGCAACTCGTCGAACTCGGTGCGGCTGGTGGAAGTGGCGCTGGGCGGCGGGGCCGGTGCGGCCCACCTGGTGGACTG
GGCCGACGACATCGACCCCGCGTGGCTGGAAGGCGTCACCACGGTGGGCGTCACCTCGGGCGCGTCCGTCCCCGAGGTCT
TGGTGCAAGGCGTGCTCGAGCGGCTGGCCGAGTGCGGCTTCGACGTGGTGCAACCGGTGACGACGGCGCAGGAGACCCTG
GTGTTCGCGTTGCCGCGGGAGATCCGCTCAGCGCGCTGA

Upstream 100 bases:

>100_bases
GCAGTAGCCATGGGTCCAAGATTGCCTCAAGCGGCGGCTAACTCCAAGCCCGCCGGCGCGAGCTGGCAAGCCCGTGTCGG
CAGAACGTACCCTGGATGCC

Downstream 100 bases:

>100_bases
TCGCCTCAGGCTGACGCCACGCGTCAGGCGTCGTACTCCCAGGATTCCGTCGGCGGCCGACCCTGCGTCCGGCCCGGCGT
CCGCGAACGGGTGCGGCGCT

Product: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 332; Mature: 331

Protein sequence:

>332_residues
MPPTVDMGIPGASSSVAVNPTRKRVLLAEPRGYCAGVDRAVETVERALEKHGPPVYVRHEIVHNRHVVSTLEKAGAVFVE
ETDEVPEGAIVVFSAHGVAPTVHAAAAERNLHTIDATCPLVTKVHNEARRFARNDYDILLIGHEGHEEVVGTAGEAPDHV
QLVDGVAAVDNVRVRDEDKVVWLSQTTLSVDETMEIVERLRQRFPKLQDPPSDDICYATQNRQVAVKAMAPECDLVIVVG
SRNSSNSVRLVEVALGGGAGAAHLVDWADDIDPAWLEGVTTVGVTSGASVPEVLVQGVLERLAECGFDVVQPVTTAQETL
VFALPREIRSAR

Sequences:

>Translated_332_residues
MPPTVDMGIPGASSSVAVNPTRKRVLLAEPRGYCAGVDRAVETVERALEKHGPPVYVRHEIVHNRHVVSTLEKAGAVFVE
ETDEVPEGAIVVFSAHGVAPTVHAAAAERNLHTIDATCPLVTKVHNEARRFARNDYDILLIGHEGHEEVVGTAGEAPDHV
QLVDGVAAVDNVRVRDEDKVVWLSQTTLSVDETMEIVERLRQRFPKLQDPPSDDICYATQNRQVAVKAMAPECDLVIVVG
SRNSSNSVRLVEVALGGGAGAAHLVDWADDIDPAWLEGVTTVGVTSGASVPEVLVQGVLERLAECGFDVVQPVTTAQETL
VFALPREIRSAR
>Mature_331_residues
PPTVDMGIPGASSSVAVNPTRKRVLLAEPRGYCAGVDRAVETVERALEKHGPPVYVRHEIVHNRHVVSTLEKAGAVFVEE
TDEVPEGAIVVFSAHGVAPTVHAAAAERNLHTIDATCPLVTKVHNEARRFARNDYDILLIGHEGHEEVVGTAGEAPDHVQ
LVDGVAAVDNVRVRDEDKVVWLSQTTLSVDETMEIVERLRQRFPKLQDPPSDDICYATQNRQVAVKAMAPECDLVIVVGS
RNSSNSVRLVEVALGGGAGAAHLVDWADDIDPAWLEGVTTVGVTSGASVPEVLVQGVLERLAECGFDVVQPVTTAQETLV
FALPREIRSAR

Specific function: Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) [H]

COG id: COG0761

COG function: function code IM; Penicillin tolerance protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispH family [H]

Homologues:

Organism=Escherichia coli, GI1786212, Length=309, Percent_Identity=50.4854368932039, Blast_Score=308, Evalue=3e-85,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003451 [H]

Pfam domain/function: PF02401 LYTB [H]

EC number: =1.17.1.2 [H]

Molecular weight: Translated: 35718; Mature: 35587

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPTVDMGIPGASSSVAVNPTRKRVLLAEPRGYCAGVDRAVETVERALEKHGPPVYVRHE
CCCCCCCCCCCCCCCEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEEEHH
IVHNRHVVSTLEKAGAVFVEETDEVPEGAIVVFSAHGVAPTVHAAAAERNLHTIDATCPL
HHHCHHHHHHHHHHCEEEEECCCCCCCCEEEEEECCCCCCHHHHHHHHCCCEEEECCCHH
VTKVHNEARRFARNDYDILLIGHEGHEEVVGTAGEAPDHVQLVDGVAAVDNVRVRDEDKV
HHHHHHHHHHHHCCCCEEEEEECCCCHHHHCCCCCCCCHHHHHHHHHHHCCCEEECCCCE
VWLSQTTLSVDETMEIVERLRQRFPKLQDPPSDDICYATQNRQVAVKAMAPECDLVIVVG
EEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCEEEEEEECCCCCEEEEEC
SRNSSNSVRLVEVALGGGAGAAHLVDWADDIDPAWLEGVTTVGVTSGASVPEVLVQGVLE
CCCCCCCEEEEEEEECCCCCHHHHHHCCCCCCHHHHCCEEEEEECCCCCHHHHHHHHHHH
RLAECGFDVVQPVTTAQETLVFALPREIRSAR
HHHHCCCHHHCCHHHHHHHEEEECCHHHHCCC
>Mature Secondary Structure 
PPTVDMGIPGASSSVAVNPTRKRVLLAEPRGYCAGVDRAVETVERALEKHGPPVYVRHE
CCCCCCCCCCCCCCEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEEEHH
IVHNRHVVSTLEKAGAVFVEETDEVPEGAIVVFSAHGVAPTVHAAAAERNLHTIDATCPL
HHHCHHHHHHHHHHCEEEEECCCCCCCCEEEEEECCCCCCHHHHHHHHCCCEEEECCCHH
VTKVHNEARRFARNDYDILLIGHEGHEEVVGTAGEAPDHVQLVDGVAAVDNVRVRDEDKV
HHHHHHHHHHHHCCCCEEEEEECCCCHHHHCCCCCCCCHHHHHHHHHHHCCCEEECCCCE
VWLSQTTLSVDETMEIVERLRQRFPKLQDPPSDDICYATQNRQVAVKAMAPECDLVIVVG
EEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCEEEEEEECCCCCEEEEEC
SRNSSNSVRLVEVALGGGAGAAHLVDWADDIDPAWLEGVTTVGVTSGASVPEVLVQGVLE
CCCCCCCEEEEEEEECCCCCHHHHHHCCCCCCHHHHCCEEEEEECCCCCHHHHHHHHHHH
RLAECGFDVVQPVTTAQETLVFALPREIRSAR
HHHHCCCHHHCCHHHHHHHEEEECCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA